BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,335,614	T→C	E609E (GAA→GAG)	gyrA ←	DNA gyrase, subunit A, type II topoisomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,335,614	0	T	C	100.0%	705.1 / NA	223	E609E (GAA→GAG)	gyrA	DNA gyrase, subunit A, type II topoisomerase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (97/126); total (97/126)

Only 100 of 231 total aligned reads displayed.

CTCGGTCACTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTC  >  NC_000913/2335524‑2335702

 tCGGTCACTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCCGACGACCGCGCGCGCCACGAGTGGCCTcc                                                                                       >  2:348382/1‑93 (MQ=255)

  cGGTCACTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCg                                                                                      <  2:818622/93‑1 (MQ=255)

  cGGTCACTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCg                                                                                      <  2:4545351/93‑1 (MQ=255)

   ggTCACTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCgg                                                                                     >  2:3155450/1‑93 (MQ=255)

   ggTCACTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCgg                                                                                     >  2:385258/1‑93 (MQ=255)

    gTCACTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGGGCCACGAGTGGGCTCCGGc                                                                                    >  1:445053/1‑93 (MQ=255)

     tCACTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCa                                                                                   >  2:4452286/1‑93 (MQ=255)

      cACTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCaa                                                                                  >  2:179405/1‑93 (MQ=255)

       ccTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAAc                                                                                 <  2:4295831/92‑1 (MQ=255)

       aCTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAAc                                                                                 <  1:3719876/93‑1 (MQ=255)

       aCTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAAc                                                                                 <  2:2063590/93‑1 (MQ=255)

       aCTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGCGGCCTCCGGCAAc                                                                                 >  1:1749085/1‑93 (MQ=255)

        cTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACt                                                                                <  2:1564724/93‑1 (MQ=255)

         tGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTg                                                                               >  2:888222/1‑93 (MQ=255)

         tGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTg                                                                               >  2:3527501/1‑93 (MQ=255)

            cAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATa                                                                            <  1:5247591/93‑1 (MQ=255)

             aGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATaa                                                                           >  1:2492926/1‑93 (MQ=255)

             aGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATaa                                                                           >  1:5737471/1‑93 (MQ=255)

              ggATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATaaa                                                                          >  2:1829428/1‑93 (MQ=255)

              ggATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGAACGCGCGCGCCACGAGTGGCCTCCGGCAACTGATaaa                                                                          >  1:3492985/1‑93 (MQ=255)

               gATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGGGGCCTCCGGCCACTGATAAAc                                                                         >  1:3430426/1‑93 (MQ=255)

                aTCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACt                                                                        >  1:4136795/1‑93 (MQ=255)

                 tCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACtt                                                                       >  1:3620749/1‑93 (MQ=255)

                 tCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACtt                                                                       <  2:5493077/93‑1 (MQ=255)

                   gCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTc                                                                     <  1:6011724/93‑1 (MQ=255)

                     aGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCAt                                                                   >  2:59626/1‑93 (MQ=255)

                      gTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATc                                                                  >  1:410951/1‑93 (MQ=255)

                       tGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCg                                                                 <  1:1515238/93‑1 (MQ=255)

                        gATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGa                                                                >  1:3903833/1‑93 (MQ=255)

                         aTACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGaa                                                               >  2:3918092/1‑93 (MQ=255)

                         aTACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGaa                                                               >  1:2738851/1‑93 (MQ=255)

                          tgcGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAAt                                                              <  2:1143528/91‑1 (MQ=255)

                          tACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAAt                                                              <  1:764947/93‑1 (MQ=255)

                           aCGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGCCCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATa                                                             >  1:4433824/1‑93 (MQ=255)

                           aCGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATa                                                             <  1:4381557/93‑1 (MQ=255)

                           aCGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATa                                                             <  2:4918251/93‑1 (MQ=255)

                            cGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAg                                                            >  1:2647853/1‑93 (MQ=255)

                            cGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAg                                                            <  1:1935994/93‑1 (MQ=255)

                             gTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGa                                                           <  1:2971974/93‑1 (MQ=255)

                             gTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGa                                                           <  2:2848563/93‑1 (MQ=255)

                              ttCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGAc                                                          <  2:2521003/93‑1 (MQ=255)

                              ttCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGAc                                                          <  2:328796/93‑1 (MQ=255)

                               tCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGAcg                                                         <  1:2333243/93‑1 (MQ=255)

                                  tCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGa                                                      <  2:742876/93‑1 (MQ=255)

                                    cTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCACGCGCCACGAGTGGCCTACGGCAACTGATAAACTTTCATCGAATAGAAGCGccc                                                    >  1:4572781/1‑93 (MQ=255)

                                     tGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCa                                                   <  1:1204736/93‑1 (MQ=255)

                                      gCTCAAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGTCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCAc                                                  <  1:787471/93‑1 (MQ=255)

                                       cTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACg                                                 <  1:869172/93‑1 (MQ=255)

                                        tCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACgg                                                <  1:2710588/93‑1 (MQ=255)

                                         ccAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGc                                               <  1:2878003/93‑1 (MQ=255)

                                         ccAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGc                                               <  2:6280524/93‑1 (MQ=255)

                                           aGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTg                                             >  2:1894321/1‑93 (MQ=255)

                                            gCGGCAGCAGTTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTgg                                            <  2:925677/93‑1 (MQ=255)

                                            gCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAGACTTTCATCGAATAGACGCGACCACGGCTgg                                            <  2:1720780/93‑1 (MQ=255)

                                             cGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGga                                           <  1:3844171/93‑1 (MQ=255)

                                             cGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGga                                           <  1:4597805/93‑1 (MQ=255)

                                               gcagcaGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGgaga                                         >  1:1399643/1‑93 (MQ=255)

                                               gcagcaGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGgaga                                         <  2:2265956/93‑1 (MQ=255)

                                                cagcagGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGaa                                        <  1:3919985/93‑1 (MQ=255)

                                                  gcagGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGc                                      <  2:5597423/93‑1 (MQ=255)

                                                     ggTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGcaca                                   >  1:5617137/1‑93 (MQ=255)

                                                     ggTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGcaca                                   <  2:4249245/93‑1 (MQ=255)

                                                     ggTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGcaca                                   <  1:2817780/93‑1 (MQ=255)

                                                     ggTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGcaca                                   >  1:423694/1‑93 (MQ=255)

                                                     ggTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGcaca                                   >  1:2177483/1‑93 (MQ=255)

                                                     ggTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGAACACGGCTGGAGGAGcaca                                   >  2:2196462/1‑93 (MQ=255)

                                                      gTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAg                                  <  1:4527134/93‑1 (MQ=255)

                                                       ttGACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGa                                 <  1:4014807/93‑1 (MQ=255)

                                                         gACGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAat                               <  2:6183727/93‑1 (MQ=255)

                                                          aCGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAata                              <  2:3015948/93‑1 (MQ=255)

                                                           cGATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAatat                             <  1:5391436/93‑1 (MQ=255)

                                                            gATCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATg                            >  1:1550258/1‑93 (MQ=255)

                                                             aTCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATgg                           <  2:5177512/93‑1 (MQ=255)

                                                             aTCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATgg                           <  2:5233489/93‑1 (MQ=255)

                                                             aTCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATgg                           >  1:6036986/1‑93 (MQ=255)

                                                              tCGGACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGt                          <  2:5414446/93‑1 (MQ=255)

                                                                ggACGACCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCg                        >  1:3297154/1‑93 (MQ=255)

                                                                    gacCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAg                    <  2:4072834/93‑1 (MQ=255)

                                                                     acagcgcgcgcCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAgt                   >  2:537969/4‑93 (MQ=255)

                                                                     acCGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAgt                   >  2:5004870/1‑93 (MQ=255)

                                                                      ccGCGCGCGCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAgtg                  <  2:5169010/93‑1 (MQ=255)

                                                                       cgcgcgcgCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAgtgt                 <  1:102408/93‑1 (MQ=255)

                                                                       cgcgcgcgCCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAgtgt                 <  1:4808395/93‑1 (MQ=255)

                                                                        gcgcgcgcCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGtt                <  1:1265330/93‑1 (MQ=255)

                                                                            gcgcCACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGcc            <  2:3294353/93‑1 (MQ=255)

                                                                               ccACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGccacc         >  1:4930600/1‑93 (MQ=255)

                                                                               ccACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGccacc         <  1:187973/93‑1 (MQ=255)

                                                                               ccACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGccaca         >  1:2860917/1‑92 (MQ=255)

                                                                                cACGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGccacca        <  1:5665279/93‑1 (MQ=255)

                                                                                 aCGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACcag       >  1:1707499/1‑93 (MQ=255)

                                                                                  cGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACcagc      <  2:2089339/93‑1 (MQ=255)

                                                                                  cGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACcagc      <  2:5828135/93‑1 (MQ=255)

                                                                                  cGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACcagc      >  2:1046253/1‑93 (MQ=255)

                                                                                  cGAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACcagc      >  2:4807453/1‑93 (MQ=255)

                                                                                   gAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACcagca     >  1:3770165/1‑93 (MQ=255)

                                                                                   gAGTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGGGAGTGTTCGCCACcagca     >  2:1275715/1‑93 (MQ=255)

                                                                                     gTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAgt   >  1:5087530/1‑93 (MQ=255)

                                                                                     gTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAgt   <  2:2727036/93‑1 (MQ=255)

                                                                                     gTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAgt   >  2:854018/1‑93 (MQ=255)

                                                                                     gTGGCCTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAgt   >  2:4644124/1‑93 (MQ=255)

CTCGGTCACTGGCAGGATCGCAGTGATACGTTCGTCCTGCTCCAGCGGCAGCAGGTTGACGATCGGACGACCGCGCGCGCCACGAGTGGCTTCCGGCAACTGATAAACTTTCATCGAATAGACGCGACCACGGCTGGAGAAGCACAGAATATGGTCGTGAGTGTTCGCCACCAGCAGTC  >  NC_000913/2335524‑2335702

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`