BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,375,891	T→C	V463V (GTA→GTG)	menD ←	2‑oxoglutarate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,375,891	0	T	C	100.0%	753.1 / NA	230	V463V (GTA→GTG)	menD	2‑oxoglutarate decarboxylase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (112/118); total (112/118)

Only 100 of 246 total aligned reads displayed.

TTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTT  >  NC_000913/2375801‑2375980

 tGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACAcc                                                                                        >  1:256528/1‑93 (MQ=255)

 tGCGACATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACAcc                                                                                        <  1:5360890/93‑1 (MQ=255)

  gCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCa                                                                                       <  1:3657888/93‑1 (MQ=255)

      cATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAgcgg                                                                                   >  1:2961569/1‑93 (MQ=255)

       aTCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAgcggc                                                                                  >  2:1045817/1‑93 (MQ=255)

        tCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAgcggcg                                                                                 >  1:186829/1‑93 (MQ=255)

        tCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAgcggcg                                                                                 <  2:566263/93‑1 (MQ=255)

         cAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGgcgc                                                                                <  1:5206852/93‑1 (MQ=255)

           gATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTTACCACAATTAACACCAGCGGCGCAg                                                                              >  1:2646138/1‑93 (MQ=255)

            aTAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGa                                                                             >  1:6082766/1‑93 (MQ=255)

            aTAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGa                                                                             <  2:3210669/93‑1 (MQ=255)

             tAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGaa                                                                            >  1:1011086/1‑93 (MQ=255)

              aGAACCGCTCACGCTCGCTTGGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGaaa                                                                           <  2:5319053/93‑1 (MQ=255)

              aGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGaaa                                                                           >  1:772071/1‑93 (MQ=255)

                 aaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCt                                                                        >  1:2702042/1‑93 (MQ=255)

                 aaCGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCt                                                                        <  1:5134490/93‑1 (MQ=255)

                  acgctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTg                                                                       >  2:2378495/1‑93 (MQ=255)

                   cgctcacgctcGCTTTGCGGCGTTGTCAGCAGCGAGAAATTTTCCCCGCCGCGGTTGTTCACCACAATTACCACCAGCGGCGCAGAAACCTGa                                                                      <  2:1979175/93‑1 (MQ=255)

                   cgctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGa                                                                      <  2:790419/93‑1 (MQ=255)

                    cctcacgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGAc                                                                     <  1:4549016/92‑1 (MQ=255)

                      tcacgctcGCTTTGCGGCGTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGc                                                                   <  2:2288170/93‑1 (MQ=255)

                        acgctcGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCaa                                                                 >  2:4924007/1‑93 (MQ=255)

                           ctcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATaa                                                              >  2:2474489/1‑93 (MQ=255)

                            tcgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAAc                                                             >  1:3622118/1‑93 (MQ=255)

                             cgctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACg                                                            <  2:3361947/93‑1 (MQ=255)

                              gctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAACGGCGCAGAAAACTGACGCCATAAAGc                                                           >  1:4302427/1‑93 (MQ=255)

                               ctTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGcc                                                          >  1:3070515/1‑93 (MQ=255)

                                 ttGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAg                                                        <  2:3062212/93‑1 (MQ=255)

                                 ttGCGCCGTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAg                                                        <  2:1838466/93‑1 (MQ=255)

                                    cggcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgcgc                                                     >  1:3395105/1‑93 (MQ=255)

                                    cggcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAgcgc                                                     <  2:647292/93‑1 (MQ=255)

                                     ggcgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCCCAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGcccgcccg                                                    >  2:4356700/1‑93 (MQ=255)

                                       cgTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt                                                  <  1:5807051/93‑1 (MQ=255)

                                       cgTGGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGtt                                                  <  2:4556163/93‑1 (MQ=255)

                                        gTTGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTTACCACCAATAAAACCCACGGGGCCCAAAACCGACGGAAAAAAGCCAGCGCGGTg                                                 >  2:4324129/1‑93 (MQ=255)

                                         ttGGCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTga                                                >  1:547625/1‑93 (MQ=255)

                                           ggCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                                              <  1:2928968/93‑1 (MQ=255)

                                           ggCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTgaga                                              >  2:5835787/1‑93 (MQ=255)

                                            gCACCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAt                                             <  2:2831371/93‑1 (MQ=255)

                                            gCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAt                                             >  2:4434526/1‑93 (MQ=255)

                                            gCAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGAGTTGAGAt                                             <  2:6149132/93‑1 (MQ=255)

                                             cAACAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGAta                                            >  2:4659298/1‑92 (MQ=255)

                                               aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                                          >  2:6245859/1‑93 (MQ=255)

                                               aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                                          <  2:1759570/93‑1 (MQ=255)

                                               aCAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGt                                          >  1:2157600/1‑93 (MQ=255)

                                                cAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa                                         <  1:1645295/93‑1 (MQ=255)

                                                cAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa                                         <  1:4125707/93‑1 (MQ=255)

                                                cAGCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa                                         <  1:1824400/93‑1 (MQ=255)

                                                cAGAGAGAAACTTGCCCCGCCGCTTTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTa                                         <  2:2188233/93‑1 (MQ=255)

                                                aaGCGAAAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCGATAACGCCAGCGCGTTGAGATCGTa                                         <  1:4482038/92‑1 (MQ=255)

                                                  gcaagaAATTTTCCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaaa                                       <  2:37773/90‑1 (MQ=255)

                                                  gCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaaa                                       <  2:248614/93‑1 (MQ=255)

                                                  gCGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTaaa                                       <  1:4151838/93‑1 (MQ=255)

                                                   cGAGAAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAg                                      >  2:3848950/1‑93 (MQ=255)

                                                    gagaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGt                                     <  2:6104955/93‑1 (MQ=255)

                                                    gagaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGt                                     <  2:2164739/93‑1 (MQ=255)

                                                    gagaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGt                                     <  1:4611565/93‑1 (MQ=255)

                                                     agaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTg                                    >  2:5090176/1‑93 (MQ=255)

                                                      gaAAATTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGc                                   >  1:5638755/1‑93 (MQ=255)

                                                      gaAAATTTCCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGc                                   <  2:2882622/93‑1 (MQ=255)

                                                      aaaaaTTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGc                                   <  2:5799185/92‑1 (MQ=255)

                                                       aaaaTTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCg                                  <  2:3994520/93‑1 (MQ=255)

                                                       aaaaTTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCg                                  >  1:5502529/1‑93 (MQ=255)

                                                        aaaTTTGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCgg                                 <  1:5024795/93‑1 (MQ=255)

                                                           tttGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgaga                              <  2:1804732/93‑1 (MQ=255)

                                                            ttGCCCGTCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagag                             >  2:5986631/1‑93 (MQ=255)

                                                             tGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagaga                            <  2:4135855/93‑1 (MQ=255)

                                                             tGCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGgagaga                            <  1:1139591/93‑1 (MQ=255)

                                                              gCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGACACCTGACGCAATAACGCCAGCGCGTTGAGCTCGTAAAGTGCGgagagag                           >  2:1540953/1‑92 (MQ=255)

                                                              gCCCGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGAt                           <  1:5942407/93‑1 (MQ=255)

                                                               cccGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATc                          >  1:3563773/1‑93 (MQ=255)

                                                               cccGCCGCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATc                          <  1:4102725/93‑1 (MQ=255)

                                                                   ccgCTGTTGTTCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGccc                      <  2:765485/93‑1 (MQ=255)

                                                                    cgctgttgttCACCTCCGTACACACCAGCGGAGCAGCAACCTGACGCAGAAACGCCAGCGCGTTGGGATCGTACAGTGCGGAGAGATCGCCca                     <  1:6310018/90‑1 (MQ=255)

                                                                    cgctgttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCca                     >  1:3828560/4‑93 (MQ=255)

                                                                    cgctgttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCca                     >  2:190005/4‑93 (MQ=255)

                                                                     gctgttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCcac                    >  1:231787/3‑93 (MQ=255)

                                                                       tgttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACaa                  >  2:2622233/1‑93 (MQ=255)

                                                                        gtttttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAAt                 <  1:1520934/93‑1 (MQ=255)

                                                                        gttgttCACCACAATTAACACCAGCGGCGCAGAACCCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAAt                 <  2:4799608/93‑1 (MQ=255)

                                                                        gttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAAt                 >  2:4047419/1‑93 (MQ=255)

                                                                        gttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAAt                 >  2:1862187/1‑93 (MQ=255)

                                                                         ttgttCACCACAATTACCACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATc                <  2:1640644/93‑1 (MQ=255)

                                                                         ttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGCTCGCCCACCATc                >  1:2729456/1‑93 (MQ=255)

                                                                         ttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATc                <  1:396974/93‑1 (MQ=255)

                                                                         ttgttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATc                <  1:330677/93‑1 (MQ=255)

                                                                           gttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGc              <  2:269681/93‑1 (MQ=255)

                                                                           gttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGc              <  2:4483371/93‑1 (MQ=255)

                                                                            ttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGcc             >  2:2063024/1‑93 (MQ=255)

                                                                            ttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGcc             >  1:3162282/1‑93 (MQ=255)

                                                                            ttCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGAAAAGTGCGGAGAGATCGCCCACAATCGcc             <  1:2594971/93‑1 (MQ=255)

                                                                             tCACCACAATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCa            >  1:3731649/1‑93 (MQ=255)

                                                                                 cacaATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGt        >  2:4403824/1‑93 (MQ=255)

                                                                                  acaATTAACGCCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCTCCAGCGTc       <  2:549125/93‑1 (MQ=255)

                                                                                  acaATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTc       >  2:2524475/1‑93 (MQ=255)

                                                                                   caATTAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCg      <  2:3606007/93‑1 (MQ=255)

                                                                                      ttAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGtt   <  2:5932546/93‑1 (MQ=255)

                                                                                      ttAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGtt   <  2:1238597/93‑1 (MQ=255)

                                                                                      atAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGtt   <  1:136632/92‑1 (MQ=255)

                                                                                       tAACACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGttt  >  1:4768808/1‑93 (MQ=255)

TTGCGGCATCAGATAGAAACGCTCACGCTCGCTTTGCGGCGTTGGCAACAGCGAGAAAATTTGCCCGCCGTTGTTGTTCACCACAATTAATACCAGCGGCGCAGAAACCTGACGCAATAACGCCAGCGCGTTGAGATCGTAAAGTGCGGAGAGATCGCCCACAATCGCCAGCGTCGGTTT  >  NC_000913/2375801‑2375980

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`