BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,419,074	A→G	N145S (AAC→AGC)	yfcG →	putative S‑transferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,419,074	0	A	G	99.6%	805.4 / ‑4.6	249	N145S (AAC→AGC)	yfcG	putative S‑transferase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (122/126); minor base C (1/0); total (123/126)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.78e-01

Only 100 of 252 total aligned reads displayed.

ATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTC  >  NC_000913/2418984‑2419163

catcattttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCa                                                                                          >  2:4612638‑M2/22‑93 (MQ=255)

  tcattttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAg                                                                                        >  1:4769216‑M2/20‑93 (MQ=255)

  tcattttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAg                                                                                        >  1:6234563‑M2/20‑93 (MQ=255)

   cattttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGc                                                                                       >  1:1230030‑M2/19‑93 (MQ=255)

   cattttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGc                                                                                       <  1:1678016‑M2/75‑1 (MQ=255)

      tttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGAGGc                                                                                    >  2:1171212‑M2/16‑93 (MQ=255)

       ttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCt                                                                                   >  1:3223942‑M2/15‑93 (MQ=255)

       ttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCt                                                                                   <  1:3018484‑M2/79‑1 (MQ=255)

       ttaatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCt                                                                                   <  2:3751956‑M2/79‑1 (MQ=255)

         aatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTgg                                                                                 <  2:297225‑M2/81‑1 (MQ=255)

         aatcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTgg                                                                                 <  1:388148‑M2/81‑1 (MQ=255)

          atcacgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGa                                                                                >  1:259677‑M2/12‑93 (MQ=255)

              cgcagccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAAc                                                                           <  2:4865643‑M2/86‑1 (MQ=255)

                   ccCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCGTGTACTGAGCAAGCGGCTGGA‑AAACTCGcc                                                                      <  2:2141783‑M2/91‑1 (MQ=255)

                    cCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTTCCATGTACTGAGCAAGCGGCTGGA‑AAACCCGccc                                                                     <  2:1433095‑M2/92‑1 (MQ=255)

                    cCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGccc                                                                     <  2:4096669‑M2/92‑1 (MQ=255)

                    cCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGccc                                                                     <  1:1851261‑M2/92‑1 (MQ=255)

                     ccccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTTAGCAAGCGGCTGGA‑AAACTCGCCct                                                                    <  1:5570414/93‑1 (MQ=255)

                     ccccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGTA‑AAACTCGCCct                                                                    >  2:2911638/1‑93 (MQ=255)

                     ccccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCct                                                                    <  1:4959763/93‑1 (MQ=255)

                     ccccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCCCCct                                                                    >  1:2845585/1‑93 (MQ=255)

                      cccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCctg                                                                   >  2:44998/1‑93 (MQ=255)

                       ccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCctgg                                                                  >  2:25662/1‑93 (MQ=255)

                       ccccAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCctgg                                                                  >  2:2743496/1‑93 (MQ=255)

                          cAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCctggctg                                                               >  2:5604835/1‑93 (MQ=255)

                           aaaCCATTCCTTACGCTATTGAGCGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCctggctgg                                                              <  2:5769885/93‑1 (MQ=255)

                           aaaCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCctggctgg                                                              >  1:3732487/1‑93 (MQ=255)

                           aaaCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCctggctgg                                                              >  1:4329349/1‑93 (MQ=255)

                             aCCATTCCTTACGCTATTGAACGTTATTAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGa                                                            >  1:4189453/1‑93 (MQ=255)

                             aCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGa                                                            <  1:5487465/93‑1 (MQ=255)

                             aCCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGa                                                            <  2:6019839/93‑1 (MQ=255)

                              ccATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAg                                                           <  2:757233/93‑1 (MQ=255)

                               cATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGAGAAACT‑GCACCGGCTGGGAgg                                                          >  2:4376486/1‑93 (MQ=255)

                                aTTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGc                                                         <  2:3580253/93‑1 (MQ=255)

                                 ttCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCg                                                        >  1:4172703/1‑93 (MQ=255)

                                  tCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCga                                                       <  2:1051686/93‑1 (MQ=255)

                                    cTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCgaga                                                     <  1:3611568/93‑1 (MQ=255)

                                    cTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCgaga                                                     <  2:2473460/93‑1 (MQ=255)

                                     ttACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGGGCCgagac                                                    >  2:1736536/1‑92 (MQ=255)

                                     ttACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGaa                                                    >  1:4402708/1‑93 (MQ=255)

                                      tACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAAc                                                   <  2:4179076/93‑1 (MQ=255)

                                       aCGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACt                                                  >  1:3071743/1‑93 (MQ=255)

                                          cTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTCGCTGGGACGCGGGAACTACa                                               >  1:5904378/1‑93 (MQ=255)

                                           tATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAg                                              >  1:5558108/1‑93 (MQ=255)

                                           tATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAg                                              <  2:6120350/93‑1 (MQ=255)

                                            aTTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGc                                             <  1:878178/93‑1 (MQ=255)

                                             ttGAACGTTATCAGGTTGAACCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCa                                            <  1:1006301/93‑1 (MQ=255)

                                               gatggTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGACTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCAtt                                          <  2:3200072/89‑1 (MQ=255)

                                               gAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCAtt                                          >  1:5328489/1‑93 (MQ=255)

                                               gAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCAtt                                          >  2:3961165/1‑93 (MQ=255)

                                               gAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCAtt                                          >  1:3542484/1‑93 (MQ=255)

                                               gAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAAATACAGAAtt                                          >  1:1095267/1‑93 (MQ=255)

                                                 aCGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGc                                        >  2:3934408/1‑93 (MQ=255)

                                                  cGTTATCAGGTTGAAACTCAGCGTCCTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCg                                       >  1:2156243/1‑93 (MQ=255)

                                                   gTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCgg                                      >  1:3782771/1‑93 (MQ=255)

                                                    ttATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGa                                     <  1:6104765/93‑1 (MQ=255)

                                                      aTCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGata                                   <  1:40450/93‑1 (MQ=255)

                                                      aTCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGata                                   <  2:5882973/93‑1 (MQ=255)

                                                      aTCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGata                                   >  1:2406921/1‑93 (MQ=255)

                                                       tCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGatat                                  >  2:5152684/1‑93 (MQ=255)

                                                        cAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATAtt                                 <  1:5153455/93‑1 (MQ=255)

                                                        cAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATAtt                                 <  2:497479/93‑1 (MQ=255)

                                                        cAGGTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATAtt                                 <  1:3403172/93‑1 (MQ=255)

                                                           gTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTTCGGATATTGcc                              >  2:2819525/1‑93 (MQ=255)

                                                           gTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGcc                              >  2:5470404/1‑93 (MQ=255)

                                                           gTTGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGcc                              >  1:5241951/1‑93 (MQ=255)

                                                            ttGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCct                             <  1:4580294/93‑1 (MQ=255)

                                                            ttGAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCct                             <  1:4477694/93‑1 (MQ=255)

                                                              gAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgc                           <  1:5611502/93‑1 (MQ=255)

                                                              gAAACTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgc                           <  1:4738052/93‑1 (MQ=255)

                                                                aaCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgctg                         <  2:202919/93‑1 (MQ=255)

                                                                aaCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgctg                         <  2:5379189/93‑1 (MQ=255)

                                                                aaCTCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCctgctg                         <  1:666704/93‑1 (MQ=255)

                                                                   tCAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGcc                      <  2:3159554/93‑1 (MQ=255)

                                                                    cAGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCg                     >  2:5620309/1‑93 (MQ=255)

                                                                     aGCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGt                    <  2:875805/93‑1 (MQ=255)

                                                                      gCGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTg                   >  1:2274816/1‑93 (MQ=255)

                                                                       cGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTgg                  <  2:2305973/93‑1 (MQ=255)

                                                                       cGTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTgg                  <  1:2444802/93‑1 (MQ=255)

                                                                        gTCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTggg                 >  1:3997682/1‑93 (MQ=255)

                                                                         tCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTTGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGt                >  1:6007060/1‑93 (MQ=255)

                                                                         tCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGt                >  2:427517/1‑93 (MQ=255)

                                                                         tCTTTACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGt                >  2:2079459/1‑93 (MQ=255)

                                                                           tttACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTa              <  1:1375920/93‑1 (MQ=255)

                                                                           tttACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTa              >  1:678619/1‑93 (MQ=255)

                                                                           tttACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTa              >  1:1911129/1‑93 (MQ=255)

                                                                            ttACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTaa             <  2:1836187/93‑1 (MQ=255)

                                                                            ttACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGGTaa             >  2:2364273/1‑93 (MQ=255)

                                                                             tACCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAAt            <  2:80200/93‑1 (MQ=255)

                                                                              aCCATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATg           >  2:1236446/1‑93 (MQ=255)

                                                                                cATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGcc         >  2:5557435/1‑93 (MQ=255)

                                                                                cATGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGcc         <  2:1870481/93‑1 (MQ=255)

                                                                                 aTGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCt        >  1:5830155/1‑93 (MQ=255)

                                                                                 aTGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCt        >  1:3351785/1‑93 (MQ=255)

                                                                                  tGTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTg       >  1:1552276/1‑93 (MQ=255)

                                                                                   gTACTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTgg      >  2:608655/1‑93 (MQ=255)

                                                                                     aCTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGAc    <  2:240023/93‑1 (MQ=255)

                                                                                     aCTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGAc    >  2:4805869/1‑93 (MQ=255)

                                                                                      cTGAGCAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACt   >  1:2481262/1‑93 (MQ=255)

                                                                                       tGAGCAAGCGGATGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTc  >  2:3678659/1‑93 (MQ=255)

ATCATCATTTTAATCACGCAGCCCCCCAAACCATTCCTTACGCTATTGAACGTTATCAGGTTGAAACTCAGCGTCTTTACCATGTACTGAACAAGCGGCTGGA‑AAACTCGCCCTGGCTGGGAGGCGAGAACTACAGCATTGCGGATATTGCCTGCTGGCCGTGGGTTAATGCCTGGACTC  >  NC_000913/2418984‑2419163

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`