BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,423,067	T→C	T63A (ACC→GCC)	hisM ←	HisM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,423,067	0	T	C	99.6%	820.2 / NA	251	T63A (ACC→GCC)	hisM	HisM
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (124/126); minor base A (1/0); total (125/126)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 255 total aligned reads displayed.

ATTCGGTTCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGTAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACC  >  NC_000913/2422977‑2423155

aTTCGGTTCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCaa                                                                                        <  2:4474313/93‑1 (MQ=255)

 ttCGGTTCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCaaa                                                                                       >  2:3788143/1‑93 (MQ=255)

 ttCGGTTCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCaaa                                                                                       >  1:1082232/1‑93 (MQ=255)

  tCGGTTCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAAt                                                                                      <  1:5254706/93‑1 (MQ=255)

   cGGTTCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATa                                                                                     <  2:3962348/93‑1 (MQ=255)

   cGGTTCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATa                                                                                     <  1:5846632/93‑1 (MQ=255)

     gTTCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAAc                                                                                   >  1:3108661/1‑93 (MQ=255)

     gTTCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAAc                                                                                   <  2:314600/93‑1 (MQ=255)

      ttCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAAcc                                                                                  >  1:3899615/1‑93 (MQ=255)

      ttCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAAcc                                                                                  <  2:5504488/93‑1 (MQ=255)

       tCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGCAAAATATAGGCAAATAACCa                                                                                 >  2:1941678/1‑93 (MQ=255)

       tCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCa                                                                                 >  1:6175110/1‑93 (MQ=255)

       tCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCa                                                                                 >  2:2271441/1‑93 (MQ=255)

       tCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATAGAGGCAAATAACCa                                                                                 >  1:3601487/1‑93 (MQ=255)

        cccTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAg                                                                                <  2:2420416/93‑1 (MQ=255)

         ccTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGa                                                                               >  2:3058818/1‑93 (MQ=255)

           ttAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGAtt                                                                             <  2:4024969/93‑1 (MQ=255)

           ttAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGAtt                                                                             >  1:4574428/1‑93 (MQ=255)

           ttAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGAtt                                                                             >  2:4215477/1‑93 (MQ=255)

              aCAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGa                                                                          >  2:3455588/1‑93 (MQ=255)

               cAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGaa                                                                         <  2:5720228/93‑1 (MQ=255)

               cAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGaa                                                                         <  2:1661735/93‑1 (MQ=255)

                aaTCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGaaa                                                                        >  2:2367092/1‑93 (MQ=255)

                 aTCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAAc                                                                       >  2:2161197/1‑93 (MQ=255)

                 aTCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAAc                                                                       <  2:5289600/93‑1 (MQ=255)

                  tctcAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACt                                                                      <  2:5990314/93‑1 (MQ=255)

                    tcAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTgg                                                                    >  1:2912089/1‑93 (MQ=255)

                    tcAAGCGTGTACATGCCGGAATAGAACACCAACAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTgg                                                                    >  2:1190014/1‑93 (MQ=255)

                     cAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGa                                                                   >  1:6209614/1‑93 (MQ=255)

                      aaGCGTGTACATGCCGTAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGAt                                                                  <  2:5451441/93‑1 (MQ=255)

                         cGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGta                                                               <  2:3584214/93‑1 (MQ=255)

                         cGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGta                                                               <  1:5990878/93‑1 (MQ=255)

                           tgtACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGtatt                                                             <  1:4400274/93‑1 (MQ=255)

                            gtACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGtattt                                                            <  1:1488459/93‑1 (MQ=255)

                             tACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGGAGAATATAGGCGAATAACGCGCTTGGAAACTGGATGtattta                                                           >  2:156317/1‑93 (MQ=255)

                             tACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGtattta                                                           <  2:1237855/93‑1 (MQ=255)

                               cATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGtatttatt                                                         <  1:4215477/93‑1 (MQ=255)

                                aTGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATttatta                                                        <  1:4851819/93‑1 (MQ=255)

                                   ccGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATTTAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTg                                                     >  1:930254/1‑93 (MQ=255)

                                     ggAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGga                                                   <  1:499896/93‑1 (MQ=255)

                                     ggAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGga                                                   >  1:5548174/1‑93 (MQ=255)

                                      gAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGgag                                                  >  1:1808730/1‑93 (MQ=255)

                                       aaTAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGgaga                                                 <  2:2972350/93‑1 (MQ=255)

                                       aaTAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGgaga                                                 >  2:4262793/1‑93 (MQ=255)

                                        aTAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGac                                                >  2:695826/1‑93 (MQ=255)

                                        aTAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGac                                                <  1:1984489/93‑1 (MQ=255)

                                        aTAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGac                                                <  2:5818494/93‑1 (MQ=255)

                                         tAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGaca                                               <  2:2209780/93‑1 (MQ=255)

                                         tAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGaca                                               >  1:2569298/1‑93 (MQ=255)

                                         aaGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGaca                                               <  2:2056162/92‑1 (MQ=255)

                                          aGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGacac                                              >  1:3295261/1‑93 (MQ=255)

                                          aGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGacac                                              <  1:4949313/93‑1 (MQ=255)

                                          aGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGacac                                              <  2:4624544/93‑1 (MQ=255)

                                            aaCACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGCAACTGGATGTATTTATTACTGGAGACACGa                                            >  1:2362187/1‑93 (MQ=255)

                                             acacCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGAc                                           >  1:4081392/1‑93 (MQ=255)

                                               acCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCa                                         <  1:2659328/93‑1 (MQ=255)

                                                ccAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCaa                                        >  1:2119040/1‑93 (MQ=255)

                                                 cAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGAAAATAACCAGATTGGAAACTGGATGTATATATTACTGGAGACACGAACAAt                                       >  2:1278761/1‑93 (MQ=255)

                                                  aGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATc                                      >  2:810/1‑93 (MQ=255)

                                                      aCTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCa                                  >  1:4752441/1‑93 (MQ=255)

                                                      aCTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCa                                  <  2:616726/93‑1 (MQ=255)

                                                       cTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAg                                 <  2:4535027/93‑1 (MQ=255)

                                                       cTGAACATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAg                                 >  1:691304/1‑93 (MQ=255)

                                                          aaCATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGACTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGaaa                              <  1:1112736/93‑1 (MQ=255)

                                                            cATACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACa                            <  2:5909042/93‑1 (MQ=255)

                                                             aTACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAg                           >  2:6243951/1‑93 (MQ=255)

                                                             aTACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAg                           <  1:5228932/93‑1 (MQ=255)

                                                              tACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAgc                          >  1:6258393/1‑93 (MQ=255)

                                                              tACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAgc                          <  1:2285441/93‑1 (MQ=255)

                                                              tACAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAgc                          <  1:5118425/93‑1 (MQ=255)

                                                               aCAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAgcg                         >  1:760145/1‑93 (MQ=255)

                                                                cAGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAgcgc                        <  1:1346971/93‑1 (MQ=255)

                                                                 aGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGcc                       <  2:4920514/93‑1 (MQ=255)

                                                                 aGCGGCGTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGcc                       >  2:3915566/1‑93 (MQ=255)

                                                                  gcggcgTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCa                      >  2:6291259/1‑93 (MQ=255)

                                                                   cggcgTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAg                     <  1:3742336/93‑1 (MQ=255)

                                                                    ggcgTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAgg                    <  2:1128326/93‑1 (MQ=255)

                                                                     gcgTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGa                   >  2:2611367/1‑93 (MQ=255)

                                                                     gcgTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGa                   >  1:3504570/1‑93 (MQ=255)

                                                                      cgTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGAc                  <  1:6031491/93‑1 (MQ=255)

                                                                       gTACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACt                 <  1:1706495/93‑1 (MQ=255)

                                                                        tACCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTc                <  1:5913502/93‑1 (MQ=255)

                                                                         aCCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTcc               >  1:2220678/1‑93 (MQ=255)

                                                                         aCCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTcc               >  1:3207568/1‑93 (MQ=255)

                                                                         aCCGCGAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTcc               <  1:6100513/93‑1 (MQ=255)

                                                                             cgAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCt           >  2:3351393/1‑93 (MQ=255)

                                                                              gAAAAATATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTa          >  1:5701213/1‑93 (MQ=255)

                                                                                aaaaTATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATc        >  2:4711590/1‑93 (MQ=255)

                                                                                aaaaTATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATc        <  2:1930990/93‑1 (MQ=255)

                                                                                aaaaTATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATc        <  1:1776282/93‑1 (MQ=255)

                                                                                  aaTATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCAc      >  2:5128493/1‑93 (MQ=255)

                                                                                  aaTATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCAc      >  2:5389959/1‑93 (MQ=255)

                                                                                  aaTATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCAc      >  1:3897319/1‑93 (MQ=255)

                                                                                  aaTATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCAc      >  2:3389627/1‑93 (MQ=255)

                                                                                  aaTATAGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCAc      <  2:942986/93‑1 (MQ=255)

                                                                                    tataGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTa    >  2:1443988/1‑93 (MQ=255)

                                                                                     ataGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTAc   >  2:4990210/1‑93 (MQ=255)

                                                                                     ataGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTAc   <  1:2811064/93‑1 (MQ=255)

                                                                                     ataGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTAc   <  1:1253477/93‑1 (MQ=255)

                                                                                      taGGCAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTAcc  >  1:371789/1‑93 (MQ=255)

ATTCGGTTCCCTTAACAATCTCAAGCGTGTACATGCCGGAATAGAACACCAGCAACTGAACATACAGCGGCGTACCGCGAAAAATATAGGTAAATAACCAGATTGGAAACTGGATGTATTTATTACTGGAGACACGACCAATCGCCAGAAACAGCGCCAGGACTCCGCCTATCACTACC  >  NC_000913/2422977‑2423155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`