BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,463,325	A→G	D2G (GAC→GGC)	yfdC →	putative transport

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,463,325	0	A	G	99.6%	847.3 / NA	266	D2G (GAC→GGC)	yfdC	putative transport
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (122/143); minor base T (0/1); total (122/144)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 268 total aligned reads displayed.

TCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACC  >  NC_000913/2463235‑2463414

tCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCa                                                                                         >  2:5268756/1‑93 (MQ=255)

 cTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCaa                                                                                        <  1:3837013/93‑1 (MQ=255)

  tACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAAc                                                                                       <  1:5075018/93‑1 (MQ=255)

  tACATTGTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAAc                                                                                       <  1:2574958/93‑1 (MQ=255)

       tttCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACaa                                                                                  >  2:1994223/1‑93 (MQ=255)

           agagTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAAtt                                                                              <  2:826956/93‑1 (MQ=255)

           agagTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAAtt                                                                              <  2:463136/93‑1 (MQ=255)

           agagTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAAtt                                                                              >  2:4695508/1‑93 (MQ=255)

            gagTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTg                                                                             >  1:3651734/1‑93 (MQ=255)

            gagTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTg                                                                             >  2:5146163/1‑93 (MQ=255)

             agTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGa                                                                            <  2:3992437/93‑1 (MQ=255)

              gTACCTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGAt                                                                           <  2:5559537/93‑1 (MQ=255)

              gTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGAt                                                                           <  1:1843636/93‑1 (MQ=255)

               tAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATc                                                                          <  2:2343374/93‑1 (MQ=255)

               tAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATc                                                                          <  2:1325884/93‑1 (MQ=255)

                aaCTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCa                                                                         <  1:2255638/93‑1 (MQ=255)

                aaCTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCa                                                                         <  1:4729620/93‑1 (MQ=255)

                   tCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAaca                                                                      >  2:1415490/1‑93 (MQ=255)

                   tCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAaca                                                                      >  2:218243/1‑93 (MQ=255)

                   tCCCGGCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAaca                                                                      <  1:991732/93‑1 (MQ=255)

                    cccGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAacac                                                                     <  1:1908011/93‑1 (MQ=255)

                     ccGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAAcaca                                                                    <  1:4849325/93‑1 (MQ=255)

                      cGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAg                                                                   <  1:2797978/93‑1 (MQ=255)

                        tCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGcg                                                                 >  2:2075715/1‑93 (MQ=255)

                         cAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGcga                                                                <  1:1294930/93‑1 (MQ=255)

                            ttGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGcgacga                                                             >  1:3394288/1‑93 (MQ=255)

                               cGAGCAAATAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAAt                                                          >  2:6164964/1‑93 (MQ=255)

                               cGAGCAAAAAAGCCGTTACGCTTTAGCTATGCGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAAt                                                          <  2:5819232/93‑1 (MQ=255)

                                gAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAAtt                                                         <  1:303339/93‑1 (MQ=255)

                                 aGCAAAAAAGCCGATACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGCAATTg                                                        >  1:838007/1‑93 (MQ=255)

                                   cAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGaa                                                      <  1:229637/93‑1 (MQ=255)

                                   cAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGaa                                                      <  1:1670333/93‑1 (MQ=255)

                                    aaaaaaGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAg                                                     <  2:268145/93‑1 (MQ=255)

                                     aaaaaGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGt                                                    >  1:1048091/1‑93 (MQ=255)

                                     aaaaaGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGt                                                    >  2:3337147/1‑93 (MQ=255)

                                      aaaaGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGtt                                                   <  1:2656950/93‑1 (MQ=255)

                                       aaaGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTg                                                  <  2:5488965/93‑1 (MQ=255)

                                       aaaGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTg                                                  >  1:2083451/1‑93 (MQ=255)

                                        aaGCCGCTACGTTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTga                                                 <  2:4575950/93‑1 (MQ=255)

                                        aaGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTga                                                 >  2:2593482/1‑93 (MQ=255)

                                        aaGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTga                                                 >  2:3095862/1‑93 (MQ=255)

                                         aGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTgag                                                >  1:1932151/1‑93 (MQ=255)

                                          gCCGCTACGCTTTAGCTATACGTGCTAATTTAAGAGGAGAGACCATGGGCAACGACAAAATTGATCACCACAGCGACGAAATTGACGTTgaga                                               >  2:2854798/1‑93 (MQ=255)

                                          gCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTgaga                                               <  2:4128725/93‑1 (MQ=255)

                                             gCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCg                                            >  2:924941/1‑93 (MQ=255)

                                             gCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCg                                            >  1:2433146/1‑93 (MQ=255)

                                              cTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCga                                           >  2:1228076/1‑93 (MQ=255)

                                               tACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCgaa                                          >  2:4871184/1‑93 (MQ=255)

                                                 cGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCgaaga                                        <  1:468031/93‑1 (MQ=255)

                                                    tttAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGaaaa                                     >  1:3254214/1‑93 (MQ=255)

                                                    tttAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGaaaa                                     >  2:1124797/1‑93 (MQ=255)

                                                    tttAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGaaaa                                     <  2:5315884/93‑1 (MQ=255)

                                                    tttAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGaaaa                                     <  1:5362853/93‑1 (MQ=255)

                                                     ttAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGaaaaa                                    <  1:928731/93‑1 (MQ=255)

                                                     ttAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGaaaaa                                    <  1:4006213/93‑1 (MQ=255)

                                                      tAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAag                                   <  1:4934651/93‑1 (MQ=255)

                                                       aGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAaga                                  <  2:153456/93‑1 (MQ=255)

                                                       aGCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAaga                                  >  2:6024665/1‑93 (MQ=255)

                                                        gCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAagag                                 >  1:2688925/1‑93 (MQ=255)

                                                        gCTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAagag                                 <  1:1624964/93‑1 (MQ=255)

                                                         cTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAgc                                <  1:540408/93‑1 (MQ=255)

                                                         cTATACGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAgc                                <  1:352332/93‑1 (MQ=255)

                                                          tataCGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAgcg                               >  1:1214600/1‑93 (MQ=255)

                                                          tataCGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAgcg                               >  2:561150/1‑93 (MQ=255)

                                                          tataCGTGCTAATTAAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAgcg                               >  2:3851334/1‑93 (MQ=255)

                                                          tagaCGTGCTAAGTCAAGAGAAGAGACTATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAgcg                               <  2:677081/90‑1 (MQ=255)

                                                           ataCGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAgcgc                              >  1:5563356/1‑93 (MQ=255)

                                                           ataCGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAgcgc                              <  1:6067197/93‑1 (MQ=255)

                                                           ataCGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAAAgcgc                              <  2:4104969/93‑1 (MQ=255)

                                                             aCGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCgg                            <  1:2864150/93‑1 (MQ=255)

                                                              cGTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGc                           <  1:2948847/93‑1 (MQ=255)

                                                               gTGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCa                          >  2:3904025/1‑93 (MQ=255)

                                                                tGCTAATTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCaa                         >  1:1300720/1‑93 (MQ=255)

                                                                    aaTTCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCaaaaaa                     >  2:563262/1‑93 (MQ=255)

                                                                      ttCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaat                   >  1:1151031/1‑93 (MQ=255)

                                                                      ttCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaat                   >  1:4468722/1‑93 (MQ=255)

                                                                       tCAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaata                  <  1:453244/93‑1 (MQ=255)

                                                                        cAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaatag                 >  2:3188089/1‑93 (MQ=255)

                                                                        cAAGAGAAGAGACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaatag                 >  1:5909261/1‑93 (MQ=255)

                                                                         aagagaagagACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaataga                >  2:3543067/1‑93 (MQ=255)

                                                                         aagagaagagACCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaataga                <  2:3691721/93‑1 (MQ=255)

                                                                          agagaagagaCCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaatagaa               <  1:3815110/93‑1 (MQ=255)

                                                                           gagaagagaCCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaatagaaa              <  1:157848/93‑1 (MQ=255)

                                                                            agaagagaCCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaatagaaat             <  1:3021704/93‑1 (MQ=255)

                                                                             gaagagaCCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaatagaaata            >  1:545640/1‑93 (MQ=255)

                                                                             gaagagaCCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaatagaaata            <  2:1695608/93‑1 (MQ=255)

                                                                             gaagagaCCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaatagaaata            >  2:2764326/1‑93 (MQ=255)

                                                                              aagagaCCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAaaatagaaatag           >  2:4366243/1‑93 (MQ=255)

                                                                               agagaCCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAaatagaaataga          <  1:6258459/93‑1 (MQ=255)

                                                                               agagaCCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAaatagaaataga          <  2:3098517/93‑1 (MQ=255)

                                                                                  gaCCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGa       <  1:4335948/93‑1 (MQ=255)

                                                                                  gaCCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGa       >  1:7698/1‑93 (MQ=255)

                                                                                   aCCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGaa      <  2:2836834/93‑1 (MQ=255)

                                                                                   aCCATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGaa      <  2:2977639/93‑1 (MQ=255)

                                                                                    ccATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAg     <  2:5437592/93‑1 (MQ=255)

                                                                                    ccATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAg     <  1:5766273/93‑1 (MQ=255)

                                                                                     cATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGa    <  2:1739053/93‑1 (MQ=255)

                                                                                     cATGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGa    >  1:2835227/1‑93 (MQ=255)

                                                                                      aTGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGAc   >  2:4935293/1‑93 (MQ=255)

                                                                                       tGGGCAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGAcc  >  2:467417/1‑93 (MQ=255)

TCTACATTTTCAGAGTAACTCCCGTCAGTTGCGAGCAAAAAAGCCGCTACGCTTTAGCTATACGTGCTAATTCAAGAGAAGAGACCATGGACAACGACAAAATTGATCAACACAGCGACGAAATTGAAGTTGAGAGCGAAGAAAAAGAGCGCGGCAAAAAAATAGAAATAGATGAAGACC  >  NC_000913/2463235‑2463414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`