BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	294,990	T→C	H444R (CAC→CGC)	intF ←	putative phage integrase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	294,990	0	T	C	100.0%	768.6 / NA	237	H444R (CAC→CGC)	intF	putative phage integrase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (125/112); total (125/112)

Only 100 of 246 total aligned reads displayed.

TATGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGTGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGCAACAATA  >  NC_000913/294900‑295079

tatGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCgcgc                                                                                         >  2:577347/1‑93 (MQ=255)

tatGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCgcgc                                                                                         >  2:1951554/1‑93 (MQ=255)

tatGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCgcgc                                                                                         >  2:4838676/1‑93 (MQ=255)

 atGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGcc                                                                                        >  1:5790687/1‑93 (MQ=255)

  tGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCa                                                                                       <  2:2101358/93‑1 (MQ=255)

   gTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCAt                                                                                      >  1:6064320/1‑93 (MQ=255)

    ttGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCAtt                                                                                     <  1:4652358/93‑1 (MQ=255)

    ttGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCAtt                                                                                     >  1:5868126/1‑93 (MQ=255)

      gATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCAtttt                                                                                   >  2:1159608/1‑93 (MQ=255)

         ttttAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGt                                                                                >  2:1206321/1‑93 (MQ=255)

         ttttAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAAGTTTCTCGCGCCATTTTCGt                                                                                <  2:6136026/93‑1 (MQ=255)

          tttAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTa                                                                               >  2:4457781/1‑93 (MQ=255)

          tttAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTa                                                                               >  2:5411982/1‑93 (MQ=255)

           ttAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTaa                                                                              <  2:3887288/93‑1 (MQ=255)

           ttAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTaa                                                                              >  1:1351999/1‑93 (MQ=255)

            tAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAAc                                                                             >  2:3430430/1‑93 (MQ=255)

              aTGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAg                                                                           >  1:2676320/1‑93 (MQ=255)

              aTGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAg                                                                           >  1:776202/1‑93 (MQ=255)

                ggAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGAt                                                                         <  2:3106905/93‑1 (MQ=255)

                 gAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATc                                                                        <  1:2779275/93‑1 (MQ=255)

                   aTCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTa                                                                      >  1:4768004/1‑93 (MQ=255)

                   aTCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTa                                                                      <  2:1700618/93‑1 (MQ=255)

                   aTCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTa                                                                      >  1:3551016/1‑93 (MQ=255)

                   aTCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTa                                                                      >  1:3617804/1‑93 (MQ=255)

                     cAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAAc                                                                    <  2:5840382/93‑1 (MQ=255)

                      aaCGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACg                                                                   >  2:353509/1‑93 (MQ=255)

                      aaCGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACg                                                                   >  2:5248794/1‑93 (MQ=255)

                       aCGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACgg                                                                  >  1:4227771/1‑93 (MQ=255)

                        ctcATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCAAACGga                                                                 <  2:557093/91‑1 (MQ=255)

                        cGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGga                                                                 <  2:1432757/93‑1 (MQ=255)

                        cGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGga                                                                 <  2:6010197/93‑1 (MQ=255)

                        cGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGga                                                                 <  1:111451/93‑1 (MQ=255)

                            tatCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGgacgac                                                             >  2:1566249/1‑93 (MQ=255)

                            tatCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGgacgac                                                             <  1:5436435/93‑1 (MQ=255)

                             atCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGacgacg                                                            >  1:2197677/1‑93 (MQ=255)

                             atCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGacgacg                                                            >  2:2303515/1‑93 (MQ=255)

                             atCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGacgacg                                                            >  2:3228788/1‑93 (MQ=255)

                              tCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACgg                                                           <  2:2953906/93‑1 (MQ=255)

                              tCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACgg                                                           <  1:2962037/93‑1 (MQ=255)

                               cAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGc                                                          <  2:3916561/93‑1 (MQ=255)

                               cAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGc                                                          >  2:4105052/1‑93 (MQ=255)

                               cAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGc                                                          <  1:3438073/93‑1 (MQ=255)

                                aaCGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCg                                                         >  1:4804557/1‑93 (MQ=255)

                                 aCGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGa                                                        >  2:4499542/1‑93 (MQ=255)

                                 aCGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGa                                                        <  1:620549/93‑1 (MQ=255)

                                 aCGTTGTTTTTTATGGGAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGa                                                        >  2:4386322/1‑93 (MQ=255)

                                  cGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGAt                                                       <  2:3363389/93‑1 (MQ=255)

                                   gttgttTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATa                                                      >  2:914562/1‑93 (MQ=255)

                                   gttgttTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATa                                                      >  2:5466965/1‑93 (MQ=255)

                                    ttgttTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAg                                                     <  1:4373707/93‑1 (MQ=255)

                                    ttgttTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAg                                                     >  1:2323225/1‑93 (MQ=255)

                                     tgttTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAgt                                                    <  2:6269863/93‑1 (MQ=255)

                                      gttTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATGTTCGTAACAGATCTAACGGACGACGGCGATAgtg                                                   >  1:3114478/1‑93 (MQ=255)

                                         ttttATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGttt                                                <  1:863116/93‑1 (MQ=255)

                                          tttATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGCTAGTGtttt                                               >  1:2873686/1‑93 (MQ=255)

                                            tATGGTGATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTc                                             <  2:4279215/93‑1 (MQ=255)

                                             aTGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCg                                            >  2:488208/1‑93 (MQ=255)

                                             aTGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCg                                            <  1:1229274/93‑1 (MQ=255)

                                               ggTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGc                                          <  1:5141444/93‑1 (MQ=255)

                                                 tAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCaa                                        >  1:5169832/1‑93 (MQ=255)

                                                  aaTACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAag                                       <  1:598597/93‑1 (MQ=255)

                                                   aTACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAaga                                      <  2:2010761/93‑1 (MQ=255)

                                                   aTACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAaga                                      <  2:2250314/93‑1 (MQ=255)

                                                   aTACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAaga                                      <  1:1773814/93‑1 (MQ=255)

                                                   aTACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAaga                                      <  2:2987338/93‑1 (MQ=255)

                                                    tACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAagag                                     <  2:2435135/93‑1 (MQ=255)

                                                     aCCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAgc                                    >  1:3057716/1‑93 (MQ=255)

                                                     aCCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAgc                                    >  1:3875772/1‑93 (MQ=255)

                                                       cTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAgcgc                                  <  1:2052577/93‑1 (MQ=255)

                                                        tGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCt                                 >  1:4676029/1‑93 (MQ=255)

                                                        tGCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCt                                 >  2:1840084/1‑93 (MQ=255)

                                                         gCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTg                                <  1:2308713/93‑1 (MQ=255)

                                                         gCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTg                                >  2:1529278/1‑93 (MQ=255)

                                                         gCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTg                                <  1:5031301/93‑1 (MQ=255)

                                                         gCTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTg                                <  2:4263051/93‑1 (MQ=255)

                                                          cTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTgg                               >  2:1774385/1‑93 (MQ=255)

                                                          cTTCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTgg                               <  1:1733741/93‑1 (MQ=255)

                                                            tCATTTAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGt                             <  2:3669122/93‑1 (MQ=255)

                                                               tttAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTtg                          <  1:1005179/93‑1 (MQ=255)

                                                               tttAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTtg                          >  1:1849958/1‑93 (MQ=255)

                                                                ttAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTtgt                         >  2:4725229/1‑93 (MQ=255)

                                                                 tAAGATCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTtgtg                        >  1:1568890/1‑93 (MQ=255)

                                                                      tCCATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCa                   <  2:2598341/93‑1 (MQ=255)

                                                                       ccATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCAt                  <  1:1936228/93‑1 (MQ=255)

                                                                        cATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTTTGTCCCATa                 >  2:5640580/1‑93 (MQ=255)

                                                                        cATGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATa                 >  2:2806483/1‑93 (MQ=255)

                                                                         aTGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATaa                >  1:3825709/1‑93 (MQ=255)

                                                                         aTGTCTCAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATaa                <  2:5724958/93‑1 (MQ=255)

                                                                             ctcAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTg            <  1:4938776/93‑1 (MQ=255)

                                                                              tcAATTTTCTCGCGCCATTTTCGTAACAGATCTCACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTgg           >  2:144225/1‑92 (MQ=255)

                                                                              tcAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGa           >  2:831873/1‑93 (MQ=255)

                                                                              tcAATTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGa           <  1:3212812/93‑1 (MQ=255)

                                                                                aaTTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGc         <  1:4486835/93‑1 (MQ=255)

                                                                                 aTTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGca        >  1:748570/1‑93 (MQ=255)

                                                                                 aTTTTCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGca        <  2:1342516/93‑1 (MQ=255)

                                                                                   tttCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGcaac      <  1:977467/93‑1 (MQ=255)

                                                                                    ttCTCGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGcaaca     <  1:2350220/93‑1 (MQ=255)

                                                                                      ctcGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGCAACAAt   <  1:5246102/93‑1 (MQ=255)

                                                                                       tcGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGCAACAATa  >  1:4202604/1‑93 (MQ=255)

                                                                                       tcGCGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGGTTGTGTCCCATAATTTGAGCAACAATa  >  1:2483273/1‑93 (MQ=255)

TATGTTGATTTTTAATGGAATCAACGCATATCAACGTTGTTTTTTATGGTAATACCTGCTTCATTTAAGATCCATGTCTCAATTTTCTCGTGCCATTTTCGTAACAGATCTAACGGACGACGGCGATAGTGTTTTTCGGCAAGAGCGCTGGGTTTGTGTCCCATAATTTGAGCAACAATA  >  NC_000913/294900‑295079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`