BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,561,679	A→G	Y28C (TAC→TGC)	b2448 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,561,679	0	A	G	99.6%	817.8 / ‑4.3	250	Y28C (TAC→TGC)	b2448	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (140/109); minor base T (0/1); total (140/110)
Fisher's exact test for biased strand distribution p-value = 4.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-01

Only 100 of 254 total aligned reads displayed.

ATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTACAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGC  >  NC_000913/2561589‑2561768

aTTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCa                                                                                         <  1:4628814/93‑1 (MQ=255)

aTTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCa                                                                                         <  2:5228391/93‑1 (MQ=255)

  tGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAgg                                                                                       <  1:3031087/93‑1 (MQ=255)

   gAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGc                                                                                      <  2:1982790/93‑1 (MQ=255)

    aaCTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCt                                                                                     >  1:3282660/1‑93 (MQ=255)

      cTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGa                                                                                   >  2:3987570/1‑93 (MQ=255)

       tgtgAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTTTCGATGTTTGTGATTTGAGGCTGaa                                                                                  >  2:4294376/1‑93 (MQ=255)

        gtgAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAAc                                                                                 <  1:1576112/93‑1 (MQ=255)

        gtgAAGGAGGACGCCATGAAACTTTTAAGCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAAc                                                                                 <  2:3472151/93‑1 (MQ=255)

         tgAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACg                                                                                >  2:2100573/1‑93 (MQ=255)

          gAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACgg                                                                               <  1:136461/93‑1 (MQ=255)

             ggaggaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACggggg                                                                            >  1:5197987/1‑92 (MQ=255)

               aggaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGggtgg                                                                          >  1:4588047/1‑93 (MQ=255)

               aggaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGggagg                                                                          >  1:1922642/1‑93 (MQ=255)

                ggaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGggagga                                                                         <  1:1014187/93‑1 (MQ=255)

                ggaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGggagga                                                                         <  1:2710931/93‑1 (MQ=255)

                 gaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGAt                                                                        >  2:2880313/1‑93 (MQ=255)

                 gaCGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGAt                                                                        >  1:3456910/1‑93 (MQ=255)

                       aTGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCa                                                                  >  1:883064/1‑93 (MQ=255)

                       aTGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCa                                                                  <  2:5573660/93‑1 (MQ=255)

                       aTGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACCGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCa                                                                  >  1:3658207/1‑93 (MQ=255)

                        tGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCaa                                                                 >  1:5030688/1‑93 (MQ=255)

                         gAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAg                                                                >  2:2420265/1‑93 (MQ=255)

                          aaaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGa                                                               <  2:2782535/93‑1 (MQ=255)

                          aaaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGa                                                               <  1:4897965/93‑1 (MQ=255)

                          aaaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGa                                                               <  1:554230/93‑1 (MQ=255)

                           aaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGaa                                                              <  1:4763361/93‑1 (MQ=255)

                           aaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGaa                                                              <  1:6037224/93‑1 (MQ=255)

                           aaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGaa                                                              >  1:1562384/1‑93 (MQ=255)

                           aaCTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGaa                                                              <  1:2343271/93‑1 (MQ=255)

                             cTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAAtt                                                            >  2:5531376/1‑93 (MQ=255)

                              ttttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTg                                                           >  2:3078871/1‑93 (MQ=255)

                              ttttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTg                                                           >  1:1053605/1‑93 (MQ=255)

                              ttttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTg                                                           >  1:409795/1‑93 (MQ=255)

                              ttttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTg                                                           >  1:1172548/1‑93 (MQ=255)

                               tttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGa                                                          >  2:424351/1‑93 (MQ=255)

                               tttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGa                                                          <  2:2532242/93‑1 (MQ=255)

                               tttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGa                                                          >  2:2442360/1‑93 (MQ=255)

                                ttAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGaa                                                         <  1:4166931/93‑1 (MQ=255)

                                  aaTCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATc                                                       <  2:2018878/93‑1 (MQ=255)

                                  aaTCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATc                                                       <  1:653119/93‑1 (MQ=255)

                                     cGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGt                                                    <  2:5833065/93‑1 (MQ=255)

                                     cGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGt                                                    >  2:4535372/1‑93 (MQ=255)

                                      gCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTc                                                   <  1:1993284/93‑1 (MQ=255)

                                       cAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCg                                                  >  2:6152128/1‑93 (MQ=255)

                                       cAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCg                                                  >  2:4630085/1‑93 (MQ=255)

                                        aaTCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGt                                                 >  2:779135/1‑93 (MQ=255)

                                        aaTCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGt                                                 <  1:3572022/93‑1 (MQ=255)

                                         atcatcCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGtt                                                >  1:3149361/1‑93 (MQ=255)

                                         atcatcCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGtt                                                >  1:1249955/1‑93 (MQ=255)

                                          tcatcCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTa                                               >  2:3001/1‑93 (MQ=255)

                                           catcCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTAc                                              <  2:2287376/93‑1 (MQ=255)

                                           catcCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTAc                                              >  1:2826389/1‑93 (MQ=255)

                                              ccTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGa                                           >  2:1020489/1‑93 (MQ=255)

                                               cTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGAt                                          >  1:3529608/1‑93 (MQ=255)

                                                tGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATa                                         >  1:1347673/1‑93 (MQ=255)

                                                tGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATa                                         >  2:6047958/1‑93 (MQ=255)

                                                tGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATa                                         >  1:2117419/1‑93 (MQ=255)

                                                 gATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATaa                                        >  2:4887885/1‑93 (MQ=255)

                                                  aTGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAAc                                       >  2:1100871/1‑93 (MQ=255)

                                                   tGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCACGAATTGAATCAGTCGTTACTGAtcaat                                      >  2:4333232/1‑89 (MQ=255)

                                                    ggTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACtg                                     >  1:3844189/1‑93 (MQ=255)

                                                     gTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACtgt                                    <  1:4827185/93‑1 (MQ=255)

                                                     gTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACtgt                                    <  2:2642367/93‑1 (MQ=255)

                                                     gTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACtgt                                    >  2:2388104/1‑93 (MQ=255)

                                                         tGACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGaaa                                >  1:5459739/1‑93 (MQ=255)

                                                          gACTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACCGTGaaaa                               >  1:5627777/1‑93 (MQ=255)

                                                           aCTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGaaaaa                              >  2:2184342/1‑93 (MQ=255)

                                                            cTGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGaaaaaa                             >  2:3647729/1‑93 (MQ=255)

                                                             tGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAAc                            <  1:2415522/93‑1 (MQ=255)

                                                             tGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAAc                            <  2:1443542/93‑1 (MQ=255)

                                                             tGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAAc                            >  1:160827/1‑93 (MQ=255)

                                                             tGGTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAAc                            <  1:3728173/93‑1 (MQ=255)

                                                              ggTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACa                           >  1:2074296/1‑93 (MQ=255)

                                                              ggTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACa                           >  1:4071605/1‑93 (MQ=255)

                                                              ggTGTTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACa                           >  2:3294842/1‑93 (MQ=255)

                                                                tgtTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGc                         >  1:1197178/1‑93 (MQ=255)

                                                                tgtTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGc                         >  1:6176952/1‑93 (MQ=255)

                                                                 gtTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGcc                        >  2:4573201/1‑93 (MQ=255)

                                                                 gtTTGCTTTGCAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGcc                        <  2:3907650/93‑1 (MQ=255)

                                                                  tttgctttgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGCTACTGATAACTGTGAAAAAACAGCCg                       >  1:3357121/1‑93 (MQ=255)

                                                                   ttgctttgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGt                      <  1:5688566/93‑1 (MQ=255)

                                                                    tgctttgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTa                     <  1:5607650/93‑1 (MQ=255)

                                                                     gctttgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTAt                    >  2:6015544/1‑93 (MQ=255)

                                                                      ctttgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTAtt                   <  1:4425475/93‑1 (MQ=255)

                                                                        ttgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTgg                 <  2:2869198/93‑1 (MQ=255)

                                                                         tgcAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGt                <  1:7108/93‑1 (MQ=255)

                                                                           cAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTCTTGGTAg              >  2:3683127/1‑93 (MQ=255)

                                                                           cAGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAg              >  2:534782/1‑93 (MQ=255)

                                                                            aGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAgg             <  1:1399118/93‑1 (MQ=255)

                                                                            aGATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAgg             >  2:339469/1‑93 (MQ=255)

                                                                             gATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAggg            >  2:12425/1‑92 (MQ=255)

                                                                             gATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGc            <  1:904842/93‑1 (MQ=255)

                                                                             gATGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGc            <  1:2219445/93‑1 (MQ=255)

                                                                               tGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCgg          >  2:5622292/1‑93 (MQ=255)

                                                                               tGTTGGTGATTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCgg          >  2:6259576/1‑93 (MQ=255)

                                                                                gTTGGTGATTGCAGGCTGAACTGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGt         >  1:5960481/1‑93 (MQ=255)

                                                                                       aTTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGc  <  1:2235076/93‑1 (MQ=255)

                                                                                       aTTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGc  >  1:3941549/1‑93 (MQ=255)

                                                                                       aTTGCAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGc  <  1:450758/93‑1 (MQ=255)

ATTGAACTGTGAAGGAGGACGCCATGAAACTTTTAATCGCAATCATCCTGATGGTGCTGACTGGTGTTTGCTTTGCAGATGTTGGTGATTACAGGCTGAACGGGGAGGATAACGCAAGAATTGAATCAGTCGTTACTGATAACTGTGAAAAAACAGCCGTATTGGTAGGCGGTGACAGGC  >  NC_000913/2561589‑2561768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`