BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,720,435	A→G	L821L (TTA→TTG)	yfiQ →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,720,435	0	A	G	99.6%	801.5 / NA	251	L821L (TTA→TTG)	yfiQ	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (131/119); minor base C (1/0); total (132/119)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 256 total aligned reads displayed.

AGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAA  >  NC_000913/2720345‑2720524

agagATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGATAGGATATAAAAGGGTTggg                                                                                         >  2:1229637/1‑93 (MQ=255)

 gagaTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGc                                                                                        <  1:670793/93‑1 (MQ=255)

 gagaTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGc                                                                                        >  1:4351838/1‑93 (MQ=255)

   gaTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCtt                                                                                      >  1:400347/1‑93 (MQ=255)

   gaTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCtt                                                                                      <  2:444809/93‑1 (MQ=255)

    aTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTa                                                                                     <  1:4630662/93‑1 (MQ=255)

    aTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTa                                                                                     >  2:2404168/1‑93 (MQ=255)

    aTCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTa                                                                                     >  2:2226117/1‑93 (MQ=255)

     tCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAg                                                                                    >  2:5086744/1‑93 (MQ=255)

      ccgcGGCGTCACGCTTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGCACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAgg                                                                                   <  2:3463478/90‑1 (MQ=255)

      ccTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAgg                                                                                   <  2:2174247/93‑1 (MQ=255)

       cTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGt                                                                                  <  2:1209874/93‑1 (MQ=255)

       cTCGGCGTCACGCGTCCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGt                                                                                  <  2:1455031/93‑1 (MQ=255)

        tCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTc                                                                                 >  2:4257970/1‑93 (MQ=255)

        tCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTc                                                                                 <  2:5857312/93‑1 (MQ=255)

         cGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCg                                                                                >  1:5308835/1‑93 (MQ=255)

          ggCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGa                                                                               >  2:2631058/1‑93 (MQ=255)

           gCGTCACGCGTGCGATTTCCGATCCTGATCACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGAc                                                                              <  2:6190804/93‑1 (MQ=255)

           gCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGAc                                                                              >  2:2755637/1‑93 (MQ=255)

           gCGTCACGCGTGCGATTTCAGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGAc                                                                              >  2:6138756/1‑93 (MQ=255)

             gTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGc                                                                            <  1:6092338/93‑1 (MQ=255)

                aCGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTa                                                                         >  1:2227146/1‑93 (MQ=255)

                aCGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTa                                                                         <  1:1473826/93‑1 (MQ=255)

                 cgcgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTaa                                                                        >  1:414142/1‑93 (MQ=255)

                 cgcgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTaa                                                                        <  2:1043314/93‑1 (MQ=255)

                 cgcgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTaa                                                                        <  2:3898530/93‑1 (MQ=255)

                 cgcgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTaa                                                                        <  2:3823557/93‑1 (MQ=255)

                 cgcgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTaa                                                                        >  2:194452/1‑93 (MQ=255)

                  gcgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAAt                                                                       <  2:5985736/93‑1 (MQ=255)

                   cgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATg                                                                      <  2:1138760/93‑1 (MQ=255)

                   cgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATg                                                                      <  1:892055/93‑1 (MQ=255)

                   cgTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATg                                                                      <  1:2206093/93‑1 (MQ=255)

                    gTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATgg                                                                     <  2:3325654/93‑1 (MQ=255)

                     tGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGa                                                                    >  1:1969257/1‑93 (MQ=255)

                     tGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGa                                                                    >  1:556694/1‑93 (MQ=255)

                      gCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGaa                                                                   <  2:3595052/93‑1 (MQ=255)

                         aTTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAg                                                                >  1:993448/1‑93 (MQ=255)

                         aTTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAg                                                                >  1:1120898/1‑93 (MQ=255)

                         aTTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAg                                                                <  2:6255776/93‑1 (MQ=255)

                             ccGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGa                                                            <  1:6194251/93‑1 (MQ=255)

                             ccGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGATCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGa                                                            >  1:4564163/1‑93 (MQ=255)

                               gATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGAtt                                                          >  1:3254774/1‑93 (MQ=255)

                               gATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGAtt                                                          <  2:36767/93‑1 (MQ=255)

                                aTCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTa                                                         >  2:4727126/1‑93 (MQ=255)

                                aTCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTa                                                         <  1:4482635/93‑1 (MQ=255)

                                 tCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTAc                                                        >  1:4295354/1‑93 (MQ=255)

                                 tCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTAc                                                        >  2:4549200/1‑93 (MQ=255)

                                 tCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCACTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTAc                                                        >  1:2548300/1‑93 (MQ=255)

                                    tGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCta                                                     >  1:4387189/1‑93 (MQ=255)

                                    tGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCta                                                     >  2:4389182/1‑93 (MQ=255)

                                     gATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCtat                                                    >  2:4123542/1‑93 (MQ=255)

                                      aTAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCtata                                                   <  1:51142/93‑1 (MQ=255)

                                         aCATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATAcgc                                                <  1:4708060/93‑1 (MQ=255)

                                           aTCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCga                                              >  1:3102479/1‑93 (MQ=255)

                                           aTCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCga                                              <  2:5801055/93‑1 (MQ=255)

                                            tCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCgag                                             >  2:5018204/1‑93 (MQ=255)

                                            tCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCgag                                             >  1:4599891/1‑93 (MQ=255)

                                            tCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCgag                                             >  1:2848181/1‑93 (MQ=255)

                                             cGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCgaga                                            <  2:4693738/93‑1 (MQ=255)

                                              gATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGAt                                           >  2:5711357/1‑93 (MQ=255)

                                              gATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGAt                                           >  2:2843795/1‑93 (MQ=255)

                                              gATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGAt                                           <  1:3094143/93‑1 (MQ=255)

                                              gATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGAt                                           <  2:2791133/93‑1 (MQ=255)

                                              gATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATCGAAAAGTTGATTACCTATACGCGAGAt                                           <  2:1886701/93‑1 (MQ=255)

                                               aTGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATc                                          >  1:5987577/1‑93 (MQ=255)

                                               aTGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATc                                          >  1:1009512/1‑93 (MQ=255)

                                                 gCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCAc                                        <  1:1562226/93‑1 (MQ=255)

                                                 gCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCAc                                        <  1:185639/93‑1 (MQ=255)

                                                   cGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACgg                                      <  1:3299892/93‑1 (MQ=255)

                                                     aaTTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGAc                                    >  1:1653391/1‑93 (MQ=255)

                                                        ttGCTGTACTGGTTCGCTCGGATCTCCAAGCGGTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATGCAGGACTAc                                 >  2:5351426/1‑93 (MQ=255)

                                                         tGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACa                                <  1:5637484/93‑1 (MQ=255)

                                                           cTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAAc                              >  2:3052765/1‑93 (MQ=255)

                                                           cTGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAAc                              <  1:2011203/93‑1 (MQ=255)

                                                            tGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACg                             >  1:1260077/1‑93 (MQ=255)

                                                            tGTACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACg                             >  2:2287685/1‑93 (MQ=255)

                                                              tACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTc                           >  1:1413578/1‑93 (MQ=255)

                                                              tACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTc                           >  2:5253292/1‑93 (MQ=255)

                                                              tACTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTc                           <  1:349978/93‑1 (MQ=255)

                                                               aCTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCt                          <  1:967588/93‑1 (MQ=255)

                                                                cTGGTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTg                         >  2:2548877/1‑93 (MQ=255)

                                                                cTGGTTCGCTCGGATCTCAAAGGGGTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTg                         >  1:2164936/1‑93 (MQ=255)

                                                                  tgTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGaa                       <  1:4256382/92‑1 (MQ=255)

                                                                  ggTTCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGaa                       >  2:3179685/1‑93 (MQ=255)

                                                                   gTTCGCTCGTATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAAt                      <  1:244522/93‑1 (MQ=255)

                                                                    ttCGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATg                     >  1:3729706/1‑93 (MQ=255)

                                                                      cGCTCGGATCTCAAGGGGGTCGGCTTAGGTCGACGCTTAATGGAAAAGTTGAGTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGt                   >  2:5773994/1‑93 (MQ=255)

                                                                      cGCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGt                   <  2:1095487/93‑1 (MQ=255)

                                                                       gCTCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTa                  <  1:1182545/93‑1 (MQ=255)

                                                                         tCGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTAtt                >  2:2969854/1‑93 (MQ=255)

                                                                          cGGATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTa               >  1:5853997/1‑93 (MQ=255)

                                                                            gATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACg             >  1:2604807/1‑93 (MQ=255)

                                                                            gATCTCAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACg             <  2:565275/93‑1 (MQ=255)

                                                                              tctcAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGAt           >  1:4604824/1‑93 (MQ=255)

                                                                              tctcAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCGGAATGGTATTACGAt           >  1:4218780/1‑93 (MQ=255)

                                                                                tcAAAGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGc         >  1:2081626/1‑93 (MQ=255)

                                                                                   aaGGGTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGCATTACGATGCCaa      >  1:4895061/1‑93 (MQ=255)

                                                                                     gggTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAAc    >  1:3746497/1‑93 (MQ=255)

                                                                                     gggTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAAc    <  2:90959/93‑1 (MQ=255)

                                                                                       gTTGGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACaa  <  1:1623062/93‑1 (MQ=255)

AGAGATCCTCGGCGTCACGCGTGCGATTTCCGATCCTGATAACATCGATGCCGAATTTGCTGTACTGGTTCGCTCGGATCTCAAAGGGTTAGGCTTAGGTCGACGCTTAATGGAAAAGTTGATTACCTATACGCGAGATCACGGACTACAACGTCTGAATGGTATTACGATGCCAAACAA  >  NC_000913/2720345‑2720524

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`