BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,751,091	G→C	A426P (GCT→CCT)	recN →	RecN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,751,091	0	G	C	99.5%	664.5 / NA	203	A426P (GCT→CCT)	recN	RecN
Reads supporting (aligned to +/- strand): ref base G (0/0); major base C (95/107); minor base A (1/0); total (96/107)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 207 total aligned reads displayed.

GATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGGCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCGGC  >  NC_000913/2751001‑2751179

 atGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTa                                                                                       <  1:2933112/93‑1 (MQ=255)

  tGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTAt                                                                                      <  2:2901629/93‑1 (MQ=255)

  tGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTAt                                                                                      <  1:4810503/93‑1 (MQ=255)

   gTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTAtt                                                                                     <  2:4042316/93‑1 (MQ=255)

    ttAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTg                                                                                    >  1:5518855/1‑93 (MQ=255)

     tAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGc                                                                                   <  2:1983285/93‑1 (MQ=255)

       aaTTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCa                                                                                 <  2:1177107/93‑1 (MQ=255)

       aaTTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCa                                                                                 <  2:1038034/93‑1 (MQ=255)

       aaTTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTACTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCa                                                                                 >  1:3388886/1‑93 (MQ=255)

        aTTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCaa                                                                                <  2:2064983/93‑1 (MQ=255)

         tttGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCaaa                                                                               <  2:6113939/93‑1 (MQ=255)

         tttGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCaaa                                                                               <  2:1779958/93‑1 (MQ=255)

           tGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGt                                                                             <  1:779250/93‑1 (MQ=255)

            gACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTc                                                                            <  2:5734404/93‑1 (MQ=255)

            gACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTc                                                                            <  1:3591442/93‑1 (MQ=255)

             aCGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCg                                                                           >  2:817449/1‑93 (MQ=255)

              cGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGc                                                                          <  2:4401732/93‑1 (MQ=255)

               gAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCa                                                                         <  2:5684905/93‑1 (MQ=255)

                aGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCAt                                                                        <  2:5451377/93‑1 (MQ=255)

                 gCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATc                                                                       >  2:3166547/1‑93 (MQ=255)

                 gCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATc                                                                       <  2:594799/93‑1 (MQ=255)

                    tCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCgg                                                                    >  2:2075748/1‑93 (MQ=255)

                    tCACCTGGGCGCTGACGGTGCCGATCGGATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCgg                                                                    >  2:1675165/1‑93 (MQ=255)

                     cACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCGAAAGTCGCATCCggt                                                                   <  2:3953381/93‑1 (MQ=255)

                     cACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGCCGCATCCggt                                                                   <  2:3854651/93‑1 (MQ=255)

                       ccTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCggtgg                                                                 <  2:2329952/93‑1 (MQ=255)

                        cTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCggtggt                                                                >  1:2106085/1‑93 (MQ=255)

                        cTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCggtggt                                                                <  2:45028/93‑1 (MQ=255)

                          gggCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGa                                                              <  2:286306/93‑1 (MQ=255)

                                  aCGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTccc                                                      <  2:5307466/93‑1 (MQ=255)

                                      tGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCAt                                                  <  1:4698002/93‑1 (MQ=255)

                                       gCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCGTCCGGTGGTGAATTGTCCCGCATc                                                 >  1:2545317/1‑93 (MQ=255)

                                       gCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATc                                                 <  1:4228219/93‑1 (MQ=255)

                                        ccGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGACTTGTCCCGCATCg                                                <  2:6190685/93‑1 (MQ=255)

                                        ccGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCg                                                >  1:258161/1‑93 (MQ=255)

                                        ccGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCg                                                >  1:969244/1‑93 (MQ=255)

                                         cGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGc                                               <  2:5816864/93‑1 (MQ=255)

                                         cGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGc                                               <  1:369123/93‑1 (MQ=255)

                                          gATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCa                                              >  1:2727210/1‑93 (MQ=255)

                                          gATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCa                                              >  1:3417603/1‑93 (MQ=255)

                                           aTCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCAc                                             >  1:531887/1‑93 (MQ=255)

                                            tCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACt                                            >  1:2854892/1‑93 (MQ=255)

                                            tCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACt                                            <  2:4199214/93‑1 (MQ=255)

                                            tCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACt                                            <  1:6204370/93‑1 (MQ=255)

                                            tCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACt                                            >  1:68957/1‑93 (MQ=255)

                                             cGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTg                                           >  2:4655214/1‑93 (MQ=255)

                                             cGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTg                                           <  1:147784/93‑1 (MQ=255)

                                              gTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTgg                                          >  1:1056126/1‑93 (MQ=255)

                                              gTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTgg                                          >  2:5192244/1‑93 (MQ=255)

                                              gTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCACACTgg                                          <  2:3420115/93‑1 (MQ=255)

                                               tATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGc                                         >  2:2665119/1‑93 (MQ=255)

                                               tATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGc                                         <  2:6237706/93‑1 (MQ=255)

                                                aTTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCGGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCa                                        >  2:3461607/1‑93 (MQ=255)

                                                aTTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCa                                        <  1:564812/93‑1 (MQ=255)

                                                aTTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCa                                        <  2:3793768/93‑1 (MQ=255)

                                                 ttGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTgccga                                       >  1:1720410/1‑89 (MQ=255)

                                                 ttGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCaa                                       <  1:4454798/93‑1 (MQ=255)

                                                  tGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAAt                                      <  2:1615120/93‑1 (MQ=255)

                                                  tGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAAt                                      >  1:4414498/1‑93 (MQ=255)

                                                  tGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAAt                                      <  2:2359127/93‑1 (MQ=255)

                                                  tGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAAt                                      <  1:3843752/93‑1 (MQ=255)

                                                   gAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATc                                     <  1:1001326/93‑1 (MQ=255)

                                                   gAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATc                                     >  1:5862086/1‑93 (MQ=255)

                                                     gTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCa                                   <  2:4587321/93‑1 (MQ=255)

                                                      tttCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAg                                  >  1:3528235/1‑93 (MQ=255)

                                                      tttCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAg                                  >  1:2296830/1‑93 (MQ=255)

                                                        tCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGt                                <  1:2976152/93‑1 (MQ=255)

                                                        tCGGGTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGt                                >  1:1816439/1‑93 (MQ=255)

                                                          gggTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGtca                              >  2:4717161/1‑93 (MQ=255)

                                                           ggTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGtcat                             >  2:2814695/1‑93 (MQ=255)

                                                           ggTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGtcat                             >  1:5063731/1‑93 (MQ=255)

                                                            gTAACCACCAACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGtcatc                            <  1:1512244/93‑1 (MQ=255)

                                                                ccaccaACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACgg                        <  1:931482/93‑1 (MQ=255)

                                                                ccaccaACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACgg                        <  1:5990767/93‑1 (MQ=255)

                                                                 caccaACCAAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGgc                       >  2:5002727/1‑93 (MQ=255)

                                                                  accaACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGgcg                      <  1:3775615/93‑1 (MQ=255)

                                                                  accaACCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGgcg                      >  1:1423686/1‑93 (MQ=255)

                                                                      aCCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTa                  <  2:1829223/93‑1 (MQ=255)

                                                                      aCCCAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTa                  <  1:3471293/93‑1 (MQ=255)

                                                                       cccAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTaa                 >  2:3289971/1‑93 (MQ=255)

                                                                         cAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTaaaa               <  2:5635531/93‑1 (MQ=255)

                                                                         cAGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTaaaa               <  1:1282270/93‑1 (MQ=255)

                                                                          aGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTaaaag              >  1:371295/1‑92 (MQ=255)

                                                                          aGGTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAAt              <  1:4471241/93‑1 (MQ=255)

                                                                            gTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATgg            >  2:4235860/1‑93 (MQ=255)

                                                                            gTCAGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGT‑CCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGa           >  1:4987431/1‑93 (MQ=255)

                                                                               aGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGaaa         <  2:1289112/93‑1 (MQ=255)

                                                                               aGCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGaaa         >  2:3656969/1‑93 (MQ=255)

                                                                                gCCAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAc        >  2:2729949/1‑93 (MQ=255)

                                                                                  cAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAccc      >  1:5747173/1‑93 (MQ=255)

                                                                                  cAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAccc      >  1:564494/1‑93 (MQ=255)

                                                                                  cAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAccc      >  2:2956671/1‑93 (MQ=255)

                                                                                  cAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAccc      >  1:3185821/1‑93 (MQ=255)

                                                                                  cAATGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAccc      >  2:4912235/1‑93 (MQ=255)

                                                                                   caTGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAcccc     <  1:1603048/92‑1 (MQ=255)

                                                                                   aaTGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAcccc     <  2:2279534/93‑1 (MQ=255)

                                                                                   aaTGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAcccc     >  2:2824894/1‑93 (MQ=255)

                                                                                   aaTGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAcccc     >  1:5380978/1‑93 (MQ=255)

                                                                                   aaTGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAAcccc     <  1:1862328/93‑1 (MQ=255)

                                                                                     tGCAGCCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCgg   <  1:2025457/93‑1 (MQ=255)

GATGTTAAATTTGACGAGCATCACCTGGGCGCTGACGGTGCCGATCGTATTGAGTTTCGGGTAACCACCAACCCAGGTCAGCCAATGCAGGCTATTGCCAAAGTCGCATCCGGTGGTGAATTGTCCCGCATCGCACTGGCAATCCAGGTCATCACGGCGCGTAAAATGGAAACCCCGGC  >  NC_000913/2751001‑2751179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`