BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,781,027	C→G	intergenic (‑151/+58)	ypjA ← / ← b2648	putative ATP‑binding component of a transport system/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,781,027	0	C	G	100.0%	721.1 / NA	235	intergenic (‑151/+58)	ypjA/b2648	putative ATP‑binding component of a transport system/hypothetical protein
Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (102/133); total (102/133)

Only 100 of 235 total aligned reads displayed.

AACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTT  >  NC_000913/2780938‑2781116

aaCGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCa                                                                                         >  2:1262594/1‑93 (MQ=255)

 aCGCAGTGCGCCGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaa                                                                                        >  1:5017604/1‑93 (MQ=25)

   gCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                                                      <  2:1603737/93‑1 (MQ=25)

       tGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaa                                                                                  <  1:1723093/93‑1 (MQ=255)

         cgcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaat                                                                                <  2:6209086/93‑1 (MQ=25)

         cgcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaat                                                                                <  2:2041026/93‑1 (MQ=25)

         cgcTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaat                                                                                <  2:2914097/93‑1 (MQ=25)

            tGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAt                                                                             <  2:5830319/93‑1 (MQ=255)

            tGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAt                                                                             <  1:2310506/93‑1 (MQ=25)

            tGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAt                                                                             >  2:3787251/1‑93 (MQ=255)

             gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAtt                                                                            <  2:1241942/93‑1 (MQ=255)

             gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAtt                                                                            <  2:3502016/93‑1 (MQ=255)

              aaGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAttt                                                                           <  2:727093/93‑1 (MQ=37)

                gtgtGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTAc                                                                         <  2:5660656/93‑1 (MQ=255)

                   tgGTTGGATAAAAAAGTCACTCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATa                                                                      >  2:6123588/1‑93 (MQ=37)

                   tgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTAGATa                                                                      >  2:4854503/1‑93 (MQ=25)

                     gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                                    <  2:4650747/93‑1 (MQ=37)

                     gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                                    >  2:5022338/1‑93 (MQ=255)

                     gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                                    >  2:1048411/1‑93 (MQ=255)

                     gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                                    <  1:5264290/93‑1 (MQ=37)

                     gTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAt                                                                    <  1:3516295/93‑1 (MQ=255)

                      ttGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                                                   >  1:677372/1‑93 (MQ=255)

                      ttGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                                                   <  1:5242689/93‑1 (MQ=255)

                      ttGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                                                   <  2:865973/93‑1 (MQ=37)

                      ttGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                                                   <  1:3304050/93‑1 (MQ=255)

                       tGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCg                                                                  >  2:1907865/1‑93 (MQ=255)

                         gATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGtt                                                                <  2:109349/93‑1 (MQ=38)

                          aTAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGttt                                                               >  1:3682043/1‑93 (MQ=255)

                           tAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTc                                                              >  2:4177687/1‑93 (MQ=255)

                           tAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTc                                                              >  1:1521816/1‑93 (MQ=255)

                            aaaaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCt                                                             <  1:2050681/93‑1 (MQ=255)

                            aaaaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCt                                                             <  1:843248/93‑1 (MQ=38)

                              aaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtga                                                           <  1:265333/93‑1 (MQ=255)

                               aaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtgat                                                          >  1:1721182/1‑93 (MQ=255)

                                aaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtgatg                                                         >  1:4554560/1‑93 (MQ=255)

                                aaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtgatg                                                         >  1:6128251/1‑93 (MQ=255)

                                  gTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGaa                                                       >  2:3367547/1‑93 (MQ=255)

                                    cAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGTCAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAata                                                     <  1:4255958/93‑1 (MQ=255)

                                    cAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAata                                                     >  2:447951/1‑93 (MQ=255)

                                    cAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAata                                                     >  1:3802559/1‑93 (MQ=255)

                                     aaTCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAatat                                                    <  1:5458928/93‑1 (MQ=255)

                                         cATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttc                                                >  2:4698940/1‑93 (MQ=255)

                                         cATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttc                                                >  1:991704/1‑93 (MQ=255)

                                         cATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttc                                                <  1:5291905/93‑1 (MQ=255)

                                          aTTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttct                                               <  2:252454/93‑1 (MQ=255)

                                           ttCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTg                                              >  1:4844300/1‑93 (MQ=255)

                                           ttCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTg                                              <  2:4926082/93‑1 (MQ=255)

                                            tCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGc                                             <  2:3862026/93‑1 (MQ=255)

                                               ggAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTca                                          >  2:2174623/1‑93 (MQ=255)

                                               ggAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTca                                          <  2:3005685/93‑1 (MQ=255)

                                               ggAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTca                                          >  1:4337889/1‑93 (MQ=255)

                                                  aaTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACAt                                       >  2:4085697/1‑93 (MQ=18)

                                                   aTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATa                                      >  1:1261934/1‑93 (MQ=18)

                                                   aTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATa                                      >  2:1577653/1‑93 (MQ=18)

                                                   aTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATa                                      >  1:2021628/1‑93 (MQ=255)

                                                    tACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaa                                     >  2:647704/1‑93 (MQ=18)

                                                    tACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaa                                     >  1:4698905/1‑93 (MQ=18)

                                                      cGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaaaa                                   <  2:3939674/93‑1 (MQ=255)

                                                      cGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaaaa                                   >  2:2288163/1‑93 (MQ=18)

                                                         gCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTCCATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATc                                <  1:3374605/93‑1 (MQ=18)

                                                          cGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATca                               <  2:1216966/93‑1 (MQ=17)

                                                           gTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcac                              <  1:4731123/93‑1 (MQ=255)

                                                            tATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcaca                             <  1:3249928/93‑1 (MQ=255)

                                                             aTTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacac                            >  1:398614/1‑93 (MQ=18)

                                                                cttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAAt                         <  1:2864023/93‑1 (MQ=255)

                                                                 ttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATa                        >  1:3095385/1‑93 (MQ=17)

                                                                  tttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATaa                       <  2:3754001/93‑1 (MQ=255)

                                                                  tttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATaa                       <  1:3941913/93‑1 (MQ=255)

                                                                   ttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAAc                      >  1:2236613/1‑93 (MQ=17)

                                                                   ttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAAc                      >  1:418287/1‑93 (MQ=17)

                                                                   ttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAAc                      <  2:5366673/93‑1 (MQ=255)

                                                                    tctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACt                     >  2:4295214/1‑93 (MQ=255)

                                                                     ctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACtt                    <  2:2383404/93‑1 (MQ=255)

                                                                      tttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACttt                   <  2:2110603/93‑1 (MQ=17)

                                                                      tttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACttt                   <  1:1588418/93‑1 (MQ=17)

                                                                      tttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACttt                   <  2:2034592/93‑1 (MQ=255)

                                                                       ttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTg                  <  1:5916706/93‑1 (MQ=255)

                                                                        tcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTga                 <  2:1935172/93‑1 (MQ=255)

                                                                          gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAANAATCACACAATAACTTTgaga               >  1:2388101/1‑93 (MQ=17)

                                                                          gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgaga               <  1:4926032/93‑1 (MQ=255)

                                                                          gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgaga               <  2:4593793/93‑1 (MQ=255)

                                                                           aCAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGAt              >  2:3220219/1‑93 (MQ=18)

                                                                           aCAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGAt              <  2:5507851/93‑1 (MQ=255)

                                                                             aaTGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCg            >  1:1071791/1‑93 (MQ=18)

                                                                             aaTGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCg            >  1:5804617/1‑93 (MQ=18)

                                                                             aaTGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCg            <  1:3535328/93‑1 (MQ=255)

                                                                               tgaggCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCa          <  1:2673071/89‑1 (MQ=255)

                                                                               tgaggCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCa          <  2:5273560/89‑1 (MQ=255)

                                                                                 aggCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGa        >  2:5999058/3‑93 (MQ=255)

                                                                                 aggCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGa        <  1:4883953/91‑1 (MQ=255)

                                                                                  ggCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGAt       <  2:2739574/92‑1 (MQ=255)

                                                                                     cGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGt    >  2:4302992/1‑93 (MQ=255)

                                                                                     cGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGt    >  2:2684133/1‑93 (MQ=255)

                                                                                      gTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGtt   <  2:1367353/93‑1 (MQ=255)

                                                                                      gTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGtt   >  1:1944394/1‑93 (MQ=255)

                                                                                      gTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGtt   <  1:2951052/93‑1 (MQ=255)

                                                                                       ttGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGttt  <  1:5712053/93‑1 (MQ=255)

                                                                                       ttGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGttt  >  1:1376258/1‑93 (MQ=255)

                                                                                       ttGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGttt  >  1:1171904/1‑93 (MQ=255)

                                                                                       ttGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGttt  >  1:2157442/1‑93 (MQ=255)

AACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCGCAGATTGTTT  >  NC_000913/2780938‑2781116

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`