BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,818,916	C→T	R373H (CGC→CAC)	alaS ←	alanyl‑tRNA synthetase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,818,916	0	C	T	99.6%	999.3 / ‑5.6	273	R373H (CGC→CAC)	alaS	alanyl‑tRNA synthetase
Reads supporting (aligned to +/- strand): ref base C (0/1); new base T (142/130); total (142/131)
Fisher's exact test for biased strand distribution p-value = 4.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.09e-01

Only 100 of 277 total aligned reads displayed.

CCATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGCGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAGAG  >  NC_000913/2818826‑2819005

ccATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCgtgc                                                                                         >  2:2277924/1‑93 (MQ=255)

    aGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCa                                                                                     <  1:5877727/93‑1 (MQ=255)

      gtgtCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCaga                                                                                   >  1:3945039/1‑93 (MQ=255)

      gtgtCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCaga                                                                                   >  1:4304018/1‑93 (MQ=255)

      gtgtCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCaga                                                                                   <  1:2030258/93‑1 (MQ=255)

       ggtCGTACAGACGGAAAGCAGTTGCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCagag                                                                                  <  2:3435550/92‑1 (MQ=255)

        gtCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGt                                                                                 <  2:1606470/93‑1 (MQ=255)

        gtCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGt                                                                                 >  2:2309393/1‑93 (MQ=255)

         tCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCATCAACGCCAGACCGTGCTCCAGAGTa                                                                                <  2:3831553/93‑1 (MQ=255)

         tCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTa                                                                                <  2:3140162/93‑1 (MQ=255)

          cGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTAc                                                                               <  1:5042852/93‑1 (MQ=255)

           gTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACg                                                                              >  2:1956404/1‑93 (MQ=255)

           gTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGAGCTCCAGAGTACg                                                                              >  1:3043142/1‑93 (MQ=255)

            tACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGa                                                                             >  1:2207247/1‑93 (MQ=255)

            tACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGa                                                                             >  2:439139/1‑93 (MQ=255)

            tACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGa                                                                             <  2:1789055/93‑1 (MQ=255)

            tACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGa                                                                             <  2:4539538/93‑1 (MQ=255)

            tACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGa                                                                             >  1:6075055/1‑93 (MQ=255)

             aCAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAg                                                                            <  2:4611979/93‑1 (MQ=255)

             aCAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAg                                                                            >  1:516966/1‑93 (MQ=255)

             aCAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAg                                                                            <  1:2404394/93‑1 (MQ=255)

               aGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCa                                                                          >  1:2791955/1‑93 (MQ=255)

               aGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCa                                                                          >  1:1295303/1‑93 (MQ=255)

                  cGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAAc                                                                       <  1:779596/93‑1 (MQ=255)

                  cGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAAc                                                                       <  1:4147522/93‑1 (MQ=255)

                   ggAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACt                                                                      >  2:2868609/1‑93 (MQ=255)

                   ggAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACt                                                                      >  1:922684/1‑93 (MQ=255)

                   ggAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACt                                                                      <  1:3866490/93‑1 (MQ=255)

                     aaaGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGc                                                                    >  1:3195369/1‑93 (MQ=255)

                      aaGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGct                                                                   >  2:504939/1‑93 (MQ=255)

                       aGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGctc                                                                  <  1:1610470/93‑1 (MQ=255)

                        gCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGctct                                                                 >  2:4841456/1‑93 (MQ=255)

                        gCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGctct                                                                 <  2:5676370/93‑1 (MQ=255)

                             ttCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCttcttc                                                            <  2:812662/93‑1 (MQ=255)

                              tCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttca                                                           >  2:4643178/1‑93 (MQ=255)

                              tCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttca                                                           >  1:3317609/1‑93 (MQ=255)

                                aCCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagt                                                         >  2:2368616/1‑93 (MQ=255)

                                aCCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagt                                                         >  2:6161659/1‑93 (MQ=255)

                                 ccATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtc                                                        <  1:5437764/93‑1 (MQ=255)

                                 ccATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtc                                                        >  1:1048474/1‑93 (MQ=255)

                                  cATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtct                                                       <  2:3994578/93‑1 (MQ=255)

                                  cATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtct                                                       <  1:5856127/93‑1 (MQ=255)

                                  cATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtct                                                       >  2:3736543/1‑93 (MQ=255)

                                   aTCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtctt                                                      >  1:4696000/1‑93 (MQ=255)

                                    tCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtcttc                                                     <  1:6007292/93‑1 (MQ=255)

                                     ccAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTtcttcagtcttca                                                    <  1:1220006/93‑1 (MQ=255)

                                       aGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGc                                                  >  1:2269569/1‑93 (MQ=255)

                                        gCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCa                                                 <  2:5355610/93‑1 (MQ=255)

                                         cGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCAc                                                >  2:5761244/1‑93 (MQ=255)

                                          gTATCGCCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGTTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCAcc                                               <  1:3373925/93‑1 (MQ=255)

                                          gTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCAcc                                               <  1:3551839/93‑1 (MQ=255)

                                           tATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCt                                              >  1:5268292/1‑93 (MQ=255)

                                           tATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCt                                              >  1:4079779/1‑93 (MQ=255)

                                             tCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGc                                            >  1:6189962/1‑93 (MQ=255)

                                               aCCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTc                                          >  1:150427/1‑93 (MQ=255)

                                                ccAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCa                                         <  2:2178953/93‑1 (MQ=255)

                                                  aGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAc                                       >  2:3323287/1‑93 (MQ=255)

                                                   gAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAcc                                      <  2:1586742/93‑1 (MQ=255)

                                                   gAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAcc                                      <  1:1440440/93‑1 (MQ=255)

                                                   gAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAcc                                      >  2:1758882/1‑93 (MQ=255)

                                                   gAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAcc                                      <  1:3667740/93‑1 (MQ=255)

                                                   gAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAAcc                                      >  1:3724259/1‑93 (MQ=255)

                                                     aaGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTg                                    <  2:2472591/93‑1 (MQ=255)

                                                      aGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTgc                                   <  2:1417712/93‑1 (MQ=255)

                                                       gTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTgcg                                  >  1:452347/1‑93 (MQ=255)

                                                         tttGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGcc                                <  2:1054075/93‑1 (MQ=255)

                                                          ttGCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCct                               <  1:3110957/93‑1 (MQ=255)

                                                            gCCAGCTCTTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCctgc                             >  2:185375/1‑93 (MQ=255)

                                                             ccAGCTCTTCATCCAGCAACACCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCctgct                            >  2:6065193/1‑93 (MQ=255)

                                                                  tctTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGt                       >  2:926708/1‑93 (MQ=255)

                                                                   ctTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGtt                      >  1:5567900/1‑93 (MQ=255)

                                                                   ctTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGtt                      >  1:5222455/1‑93 (MQ=255)

                                                                   ctTCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGtt                      <  1:3607919/93‑1 (MQ=255)

                                                                     tCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTc                    <  1:2090100/93‑1 (MQ=255)

                                                                     tCATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTc                    <  1:2744105/93‑1 (MQ=255)

                                                                      cATCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCa                   <  1:5316026/93‑1 (MQ=255)

                                                                       aTCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAg                  <  2:5971247/93‑1 (MQ=255)

                                                                       aTCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAg                  <  2:5161047/93‑1 (MQ=255)

                                                                        tCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAACCTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAgg                 <  2:6309000/93‑1 (MQ=255)

                                                                        tCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAgg                 >  2:6238461/1‑93 (MQ=255)

                                                                        tCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAgg                 <  2:1307828/93‑1 (MQ=255)

                                                                        tCCAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAgg                 <  1:2528812/93‑1 (MQ=255)

                                                                         ccAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGt                >  2:5208660/1‑93 (MQ=255)

                                                                          cAGCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTc               >  2:2838413/1‑93 (MQ=255)

                                                                            gCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCtt             >  2:3002700/1‑93 (MQ=255)

                                                                            gCAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCtt             >  2:3335631/1‑93 (MQ=255)

                                                                             cAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTc            <  2:5270740/93‑1 (MQ=255)

                                                                             cAACGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTc            >  1:1100466/1‑93 (MQ=255)

                                                                              aaCGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCGGGTCTTCa           <  1:1736252/93‑1 (MQ=255)

                                                                              aaCGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCa           >  1:4924727/1‑93 (MQ=255)

                                                                              aaCGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCa           >  1:1895987/1‑93 (MQ=255)

                                                                               aCGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCAc          >  1:4052589/1‑93 (MQ=255)

                                                                               aCGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCAc          >  2:1093551/1‑93 (MQ=255)

                                                                               aCGCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCAc          >  2:3648333/1‑93 (MQ=255)

                                                                                 gCCAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCAccc        <  1:4791253/93‑1 (MQ=255)

                                                                                  ccAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCg       <  1:2512577/93‑1 (MQ=255)

                                                                                   cAGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGc      >  1:2666546/1‑93 (MQ=255)

                                                                                    aGACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCa     <  2:1835811/93‑1 (MQ=255)

                                                                                     gACCGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCag    >  2:277595/1‑93 (MQ=255)

                                                                                       ccGTGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCagag  >  2:697811/1‑93 (MQ=255)

CCATAGGTGTCGTACAGACGGAAAGCAGTTTCACCATCCAGCGTATCACCAGAAAGTTTTGCCAGCTCTTCATCCAGCAACGCCAGACCGCGCTCCAGAGTACGAGCAAACTGCTCTTCTTCAGTCTTCAGCACCTGCTCAACCTGCGCCTGCTGGCGTTTCAGGTCTTCACCCGCAGAG  >  NC_000913/2818826‑2819005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`