BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,824,797	T→C	F128S (TTT→TCT)	srlE →	PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,824,797	0	T	C	100.0%	753.2 / NA	233	F128S (TTT→TCT)	srlE	PTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (111/122); total (111/122)

Only 100 of 236 total aligned reads displayed.

GCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA  >  NC_000913/2824708‑2824886

gCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTc                                                                                        <  2:5490215/93‑1 (MQ=255)

  tGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCg                                                                                      >  1:5009846/1‑93 (MQ=255)

   ggCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGt                                                                                     <  1:4659134/93‑1 (MQ=255)

   ggCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGt                                                                                     >  2:2717329/1‑93 (MQ=255)

   ggCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGt                                                                                     <  2:3710721/93‑1 (MQ=255)

    gcgcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTg                                                                                    <  1:1726845/93‑1 (MQ=255)

      gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  >  1:2863294/1‑93 (MQ=255)

      gcAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTggg                                                                                  >  1:5817199/1‑93 (MQ=255)

       cAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGc                                                                                 >  1:3263579/1‑93 (MQ=255)

        aGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGcc                                                                                >  2:2571177/1‑93 (MQ=255)

          tACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                                              >  2:1872269/1‑93 (MQ=255)

          tACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGt                                                                              <  1:3180120/93‑1 (MQ=255)

           aCATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTg                                                                             >  1:2028884/1‑93 (MQ=255)

             aTTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGAc                                                                           >  2:817950/1‑93 (MQ=255)

              ttGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACt                                                                          <  1:472306/93‑1 (MQ=255)

               tgtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCTCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                                         <  1:3612345/93‑1 (MQ=255)

               tgtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTa                                                                         <  1:33841/93‑1 (MQ=255)

                gtgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTAt                                                                        >  2:1040756/1‑93 (MQ=255)

                 tgGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATg                                                                       >  2:1091364/1‑93 (MQ=255)

                  ggAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGa                                                                      >  2:5666844/1‑93 (MQ=255)

                  ggAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGa                                                                      >  1:5325303/1‑93 (MQ=255)

                  ggAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGa                                                                      >  2:6078668/1‑93 (MQ=255)

                  ggAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGa                                                                      >  1:4278932/1‑93 (MQ=255)

                   gAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCGCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGac                                                                     <  1:318153/93‑1 (MQ=255)

                    aaGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGaca                                                                    <  2:3870462/93‑1 (MQ=255)

                    aaGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGaca                                                                    <  1:4483016/93‑1 (MQ=255)

                      gATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAcc                                                                  <  1:6145905/93‑1 (MQ=255)

                      gATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACAcc                                                                  <  2:1469621/93‑1 (MQ=255)

                       atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                                                 <  2:5468638/93‑1 (MQ=255)

                       atatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCa                                                                 <  2:3808052/93‑1 (MQ=255)

                        tatTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAg                                                                <  1:3555863/93‑1 (MQ=255)

                         atTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGt                                                               <  1:2959455/93‑1 (MQ=255)

                          tttATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTa                                                              >  2:2980248/1‑93 (MQ=255)

                            tATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                                            >  1:537749/1‑93 (MQ=255)

                            tATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAg                                                            <  2:4340098/93‑1 (MQ=255)

                              tggctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGaa                                                          >  2:2841189/4‑93 (MQ=255)

                                 ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       <  1:4932377/93‑1 (MQ=255)

                                 ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       <  1:5072684/93‑1 (MQ=255)

                                 ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       >  1:4513870/1‑93 (MQ=255)

                                 ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       <  1:3791131/93‑1 (MQ=255)

                                 ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       >  1:5802308/1‑93 (MQ=255)

                                 ctctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAAACCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAAt                                                       <  1:4775013/93‑1 (MQ=255)

                                  tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      >  1:1351347/1‑93 (MQ=255)

                                  tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      >  1:602327/1‑93 (MQ=255)

                                  tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      >  1:5599363/1‑93 (MQ=255)

                                  tctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATc                                                      >  1:3767202/1‑93 (MQ=255)

                                   ctGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCa                                                     <  2:4622201/93‑1 (MQ=255)

                                     ggCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCAcc                                                   >  2:4846235/1‑93 (MQ=255)

                                      gCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCg                                                  <  2:1636048/93‑1 (MQ=255)

                                       tgTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGa                                                 <  2:2117772/92‑1 (MQ=255)

                                        gTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGaa                                                <  2:1422377/93‑1 (MQ=255)

                                         tAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAAc                                               >  1:4000178/1‑93 (MQ=255)

                                            aagaagAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACaaa                                            <  2:6020832/93‑1 (MQ=255)

                                             agaataAAACATCACTGTAGTAGGTGATGCGACACCACATCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                                           <  1:1590626/93‑1 (MQ=255)

                                             agaagaAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAg                                           <  2:4682182/93‑1 (MQ=255)

                                              gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                          >  1:2511160/1‑93 (MQ=255)

                                              gaagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGc                                          >  1:4631430/1‑93 (MQ=255)

                                               aagaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCg                                         <  2:398905/93‑1 (MQ=255)

                                                 gaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                       >  1:3202320/1‑93 (MQ=255)

                                                 gaaAACATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGAt                                       <  1:2428571/93‑1 (MQ=255)

                                                  aaaaCATCACTGTAGTAGGTGATGCGACACCACAGCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATg                                      <  2:741607/93‑1 (MQ=255)

                                                  aaaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATg                                      <  1:1139937/93‑1 (MQ=255)

                                                   aaaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTGCCGTGGGCCGTGACTATGACGCCAGTAAGAAAACCACCGAACAAAGCGATgg                                     <  2:4302609/93‑1 (MQ=255)

                                                    aaCATCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGt                                    >  2:4958891/1‑93 (MQ=255)

                                                       aTCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTa                                 <  1:5937160/93‑1 (MQ=255)

                                                        tCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGTTGGTTTAc                                <  2:6155988/93‑1 (MQ=255)

                                                        tCACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTAc                                <  2:2472339/93‑1 (MQ=255)

                                                         cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACt                               <  1:743202/93‑1 (MQ=255)

                                                         cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACt                               >  1:5552713/1‑93 (MQ=255)

                                                         cACTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACt                               <  1:6078668/93‑1 (MQ=255)

                                                          aCTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTg                              <  1:2058536/93‑1 (MQ=255)

                                                           cTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg                             <  1:1188399/93‑1 (MQ=255)

                                                           cTGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTgg                             <  1:4220125/93‑1 (MQ=255)

                                                            tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGc                            <  2:3522822/93‑1 (MQ=255)

                                                            tGTAGTAGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGc                            <  1:4796333/93‑1 (MQ=255)

                                                             gtagtaGGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCg                           >  2:5534762/1‑93 (MQ=255)

                                                               agtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGaa                         >  1:1483432/1‑93 (MQ=255)

                                                                gtagGTGTTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAg                        <  1:265742/93‑1 (MQ=255)

                                                                gtagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAg                        >  2:337972/1‑93 (MQ=255)

                                                                 tagGTGATGCGACACCACACCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAgg                       <  1:2222486/93‑1 (MQ=255)

                                                                 tagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAgg                       <  2:3108778/93‑1 (MQ=255)

                                                                 tagGTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAgg                       >  1:6191585/1‑93 (MQ=255)

                                                                   ggTGATGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTg                     >  1:2770993/1‑93 (MQ=255)

                                                                       aTGCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGaa                 <  1:3468966/93‑1 (MQ=255)

                                                                         gCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATg               <  2:1930216/93‑1 (MQ=255)

                                                                         gCGACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATg               <  2:2659047/93‑1 (MQ=255)

                                                                           gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg             >  2:2325637/1‑93 (MQ=255)

                                                                           gACACCACAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATggg             <  1:1658518/93‑1 (MQ=255)

                                                                             caccacACCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa           <  2:455397/93‑1 (MQ=255)

                                                                             caccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCa           >  1:5204903/1‑93 (MQ=255)

                                                                              accacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCAt          <  2:3286667/93‑1 (MQ=255)

                                                                               ccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATg         <  2:5116654/93‑1 (MQ=255)

                                                                               ccacAACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATg         <  1:847388/93‑1 (MQ=255)

                                                                                 acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg       >  2:5364935/1‑93 (MQ=255)

                                                                                 acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg       <  1:335414/93‑1 (MQ=255)

                                                                                 acaACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATggg       >  2:5889385/1‑93 (MQ=255)

                                                                                  caACCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATgggg      <  2:367212/93‑1 (MQ=255)

                                                                                   aaCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGt     >  1:2357932/1‑93 (MQ=255)

                                                                                    aCCCTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTc    >  1:3710720/1‑93 (MQ=255)

                                                                                     cccTCTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTcc   <  1:5530689/93‑1 (MQ=255)

GCTGGCGCAGTACATTGTGGAAGATATTTATGTCTCTGGCGTAAAAGAAGAAAACATCACTGTAGTAGGTGATGCGACACCACAACCCTTTTCCGTGGGCCGTGACTATGACACCAGTAAGAAAATCACCGAACAAAGCGATGGTTTACTGGCGAAGGTGGGAATGGGCATGGGGTCCA  >  NC_000913/2824708‑2824886

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`