BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,825,077	T→C	C221C (TGT→TGC)	srlE →	PTS system glucitol/sorbitol‑specific enzyme IIBC component

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,825,077	0	T	C	100.0%	690.3 / NA	215	C221C (TGT→TGC)	srlE	PTS system glucitol/sorbitol‑specific enzyme IIBC component
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (80/135); total (80/135)

Only 100 of 230 total aligned reads displayed.

TATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCG  >  NC_000913/2824987‑2825165

tATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGctc                                                                                        >  1:4463470/1‑93 (MQ=255)

 aTGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGctcc                                                                                       <  1:4653176/93‑1 (MQ=255)

  tGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGctcct                                                                                      >  1:4428700/1‑93 (MQ=255)

   ggCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGctcctt                                                                                     >  2:1023180/1‑93 (MQ=255)

    gCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGctccttc                                                                                    >  1:3280718/1‑93 (MQ=255)

     cTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGctccttcc                                                                                   >  2:1698093/1‑93 (MQ=255)

      ttCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTccc                                                                                  >  1:5955637/1‑93 (MQ=255)

       tCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCa                                                                                 >  2:1137241/1‑93 (MQ=255)

       tCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCa                                                                                 <  1:3742591/93‑1 (MQ=255)

        cTGACCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTcccgc                                                                                <  2:3126143/93‑3 (MQ=255)

          ggCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTg                                                                              >  1:2469816/1‑93 (MQ=255)

          ggCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTg                                                                              <  1:4078774/93‑1 (MQ=255)

           gCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGc                                                                             >  1:5823779/1‑93 (MQ=255)

           gCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGc                                                                             <  2:5445762/93‑1 (MQ=255)

           gCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGc                                                                             >  2:2332516/1‑93 (MQ=255)

           gCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGc                                                                             <  2:2556975/93‑1 (MQ=255)

             cTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCtt                                                                           >  1:4271531/1‑93 (MQ=255)

              ttGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCttt                                                                          >  1:2106570/1‑93 (MQ=255)

              ttGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCttt                                                                          <  1:4363249/93‑1 (MQ=255)

                ggTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCa                                                                        >  2:4463931/1‑93 (MQ=255)

                 gTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCAc                                                                       >  1:1370443/1‑93 (MQ=255)

                  tGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCAcc                                                                      <  2:4667223/93‑1 (MQ=255)

                  ggACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCAcc                                                                      <  1:1511838/92‑1 (MQ=255)

                   gACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCt                                                                     <  1:3237728/93‑1 (MQ=255)

                   gACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCt                                                                     >  2:2207561/1‑93 (MQ=255)

                   gACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCt                                                                     <  2:2407900/93‑1 (MQ=255)

                     cTGGATTGCCCACGCTCTTTCTCCGCTGTCGAGCCATCCACTCGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCttt                                                                   <  1:2841189/93‑1 (MQ=255)

                      tGGATTGCCCACAGTCTTGCTCCCCTGGCGGGCCATCCACTGGGTCTGGTAATACTCGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTttc                                                                  <  2:1754478/93‑1 (MQ=255)

                       ggATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTttcc                                                                 >  2:1868668/1‑92 (MQ=255)

                       ggATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTttcc                                                                 <  1:337972/93‑2 (MQ=255)

                       ggATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTttcc                                                                 <  1:1263114/93‑2 (MQ=255)

                       cgATTCCCTACGGTTTCCCTCTGGTGGGGGGCCATCGAGTGGGTCTGGTCATGCTGGGGCGTATCTGCTCCTTCCCACTGCTCTCACCTttcc                                                                 <  2:602327/92‑2 (MQ=255)

                         aTTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTttcctc                                                               >  2:4373132/1‑93 (MQ=255)

                         aTTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTttcctc                                                               <  1:1677883/93‑1 (MQ=255)

                          ttGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCg                                                              <  2:3053552/93‑1 (MQ=255)

                           tGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCgg                                                             <  2:5645864/93‑1 (MQ=255)

                            gCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGc                                                            >  2:2350670/1‑93 (MQ=255)

                             cccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGcc                                                           <  1:5862495/93‑1 (MQ=255)

                              ccACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGccc                                                          <  2:3815614/93‑1 (MQ=255)

                                aCGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAg                                                        <  1:5534762/93‑1 (MQ=255)

                                 cGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAgg                                                       <  1:1057142/93‑1 (MQ=255)

                                 cGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAgg                                                       >  1:5429262/1‑93 (MQ=255)

                                  ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                      <  2:5612583/93‑1 (MQ=255)

                                  ggTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgc                                                      <  1:3981441/93‑1 (MQ=255)

                                   gTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTAATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcg                                                     >  2:5025573/1‑93 (MQ=255)

                                    tCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGgcgc                                                    <  1:2316004/93‑1 (MQ=255)

                                     cTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCa                                                   <  2:6229081/93‑1 (MQ=255)

                                       tGCTCCGCTGTCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGt                                                 <  1:5624896/93‑1 (MQ=255)

                                        gCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGtt                                                >  1:2351337/1‑93 (MQ=255)

                                        gCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGtt                                                >  2:68517/1‑93 (MQ=255)

                                         cTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTa                                               <  1:3672210/93‑1 (MQ=255)

                                          gccGCTGGCGAGACATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTAt                                              <  1:3882247/92‑1 (MQ=255)

                                          cccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTAt                                              <  2:3668744/92‑1 (MQ=255)

                                           ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                             <  1:130793/93‑1 (MQ=255)

                                           ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                             <  2:5839470/93‑1 (MQ=255)

                                           ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                             >  1:480734/1‑93 (MQ=255)

                                           ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                             <  1:5764973/93‑1 (MQ=255)

                                           ccGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATc                                             <  1:5042645/93‑1 (MQ=255)

                                               tGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcac                                         <  2:1190015/93‑1 (MQ=255)

                                               gggCGAGCTATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACGGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcac                                         <  2:713889/92‑1 (MQ=255)

                                                ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                        >  1:261856/1‑93 (MQ=255)

                                                ggCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGcaca                                        <  2:607874/93‑1 (MQ=255)

                                                 gCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAg                                       <  1:1091364/93‑1 (MQ=255)

                                                 gCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAg                                       <  1:3737082/93‑1 (MQ=255)

                                                   gAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGt                                     <  2:1294641/93‑1 (MQ=255)

                                                     gCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTa                                   <  1:609451/93‑1 (MQ=255)

                                                       cATCCACTGGGTCTGGTCATGCTGGCTCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                 <  2:5820651/93‑1 (MQ=255)

                                                       cATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATc                                 >  2:3551739/1‑93 (MQ=255)

                                                         tCCACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCgg                               <  1:2732937/93‑1 (MQ=255)

                                                           cACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCATAGGTTATCGGCg                             >  1:3150206/1‑93 (MQ=255)

                                                           cACTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCg                             >  1:899416/1‑93 (MQ=255)

                                                             cTGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTa                           <  1:2171893/93‑1 (MQ=255)

                                                              tGGGTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTat                          >  2:252914/1‑93 (MQ=255)

                                                               gggTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTatt                         <  2:3367575/93‑1 (MQ=255)

                                                               gggTCTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTatt                         <  2:4880024/93‑1 (MQ=255)

                                                                   cTGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgatt                     <  1:4907450/93‑1 (MQ=255)

                                                                    tGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                    >  2:2133424/1‑93 (MQ=255)

                                                                    tGGTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTattgattg                    <  1:53808/93‑1 (MQ=255)

                                                                     ggTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTgg                   >  2:2967549/1‑93 (MQ=255)

                                                                      gTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGc                  >  1:5195070/1‑93 (MQ=255)

                                                                      gTCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGc                  <  1:6268736/93‑1 (MQ=255)

                                                                       tCATGCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCg                 <  1:2448410/93‑1 (MQ=255)

                                                                           gCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCa             <  2:4965191/93‑1 (MQ=255)

                                                                           gCTGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCa             <  2:6120972/93‑1 (MQ=255)

                                                                             tGGCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGa           <  2:4109070/93‑1 (MQ=255)

                                                                              ggCGCTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAt          >  1:1655582/1‑93 (MQ=255)

                                                                               gcgcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAtt         <  2:4513870/93‑1 (MQ=255)

                                                                               gcgcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAtt         <  2:5477932/93‑1 (MQ=255)

                                                                               gcgcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAtt         <  2:564542/93‑1 (MQ=255)

                                                                               gcgcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGAtt         <  2:5964456/93‑1 (MQ=255)

                                                                                cgcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACCGGTTATCGGCGTATTGATTGGCGTGCAGATTg        >  1:2907022/1‑93 (MQ=255)

                                                                                 gcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTgg       >  1:3440852/1‑93 (MQ=255)

                                                                                 gcTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTgg       <  2:550745/93‑1 (MQ=255)

                                                                                  cTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGTCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGAGTGGCGTGCAGATTggc      >  2:3443839/1‑92 (MQ=255)

                                                                                  cTCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGt      <  2:839251/93‑1 (MQ=255)

                                                                                   tCATCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGATATCGGCGTATTGATTGGCGTGCAGATTGGtc     >  1:1183824/1‑93 (MQ=255)

                                                                                     aTCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtctc   >  1:1914571/1‑93 (MQ=255)

                                                                                     aTCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGtctc   >  1:1167807/1‑93 (MQ=255)

                                                                                      tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTTGCGTGCAGATTGGTCTCg  >  2:482932/1‑93 (MQ=255)

                                                                                      tCTGCTCCTTCCCACTGCTTTCACCTTTCCTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCg  <  1:2649381/93‑1 (MQ=255)

TATGGCTTCTGGCCTTGGTGACTGGATTGCCCACGGTCTTGCTCCGCTGGCGAGCCATCCACTGGGTCTGGTCATGCTGGCGCTCATCTGTTCCTTCCCACTGCTTTCACCTTTCTTCGGCCCAGGCGCAGTTATCGCACAGGTTATCGGCGTATTGATTGGCGTGCAGATTGGTCTCG  >  NC_000913/2824987‑2825165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`