BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,844,596	A→G	R280R (CGT→CGC)	hycD ←	membrane‑spanning protein of hydrogenase 3

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,844,596	0	A	G	100.0%	604.3 / NA	189	R280R (CGT→CGC)	hycD	membrane‑spanning protein of hydrogenase 3
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (108/81); total (108/81)

Only 100 of 194 total aligned reads displayed.

CTTTAATCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACC  >  NC_000913/2844506‑2844685

 tttAATCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCgg                                                                                        <  1:5114775/93‑1 (MQ=255)

    aaTCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGcc                                                                                     >  2:4186779/1‑93 (MQ=255)

     aTCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCa                                                                                    >  2:4812348/1‑93 (MQ=255)

     aTCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCa                                                                                    <  2:3310963/93‑1 (MQ=255)

        aCGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGc                                                                                 <  2:2569500/93‑1 (MQ=255)

        aCGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGc                                                                                 >  2:2479497/1‑93 (MQ=255)

        aCGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGCAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATgt                                                                                 >  2:4987066/1‑92 (MQ=255)

        aCGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGCAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATga                                                                                 >  1:1675668/1‑92 (MQ=255)

         cgccgcCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCt                                                                                <  1:5767580/93‑1 (MQ=255)

           ccgccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGt                                                                              <  1:3632976/93‑1 (MQ=255)

            cgccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGtt                                                                             >  2:3507858/1‑93 (MQ=255)

              ccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGtttt                                                                           >  1:3105064/1‑93 (MQ=255)

              ccAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGtttt                                                                           <  1:2846446/93‑1 (MQ=255)

                aGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCg                                                                         >  1:5491794/1‑93 (MQ=255)

                aGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGACATGCTGTTTTCg                                                                         >  1:3251332/1‑93 (MQ=255)

                 gCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGa                                                                        >  1:1877209/1‑93 (MQ=255)

                 gCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGa                                                                        <  2:1896168/93‑1 (MQ=255)

                  cAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGaa                                                                       <  2:1668903/93‑1 (MQ=255)

                   aaGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGaaa                                                                      >  2:1462267/1‑92 (MQ=255)

                   aaGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAAc                                                                      <  2:6086611/93‑1 (MQ=255)

                    aGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACa                                                                     <  2:2245649/93‑1 (MQ=255)

                    aGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACa                                                                     <  2:1883998/93‑1 (MQ=255)

                     ggAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAg                                                                    <  1:3225857/93‑1 (MQ=255)

                     ggAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAg                                                                    >  1:5542759/1‑93 (MQ=255)

                        gaCGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAgcgc                                                                 <  2:1993810/93‑1 (MQ=255)

                        gaCGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAgcgc                                                                 <  1:474790/93‑1 (MQ=255)

                        gaCGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAgcgc                                                                 >  1:350170/1‑93 (MQ=255)

                        aaCGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAgcgc                                                                 <  1:4517356/92‑1 (MQ=255)

                         aCGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGcgcg                                                                >  1:770378/1‑93 (MQ=255)

                           gAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGAt                                                              >  1:913482/1‑93 (MQ=255)

                           gAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGAAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGGGAt                                                              >  2:5745294/1‑93 (MQ=255)

                            aaCGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATa                                                             >  2:3355629/1‑93 (MQ=255)

                             aCGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATaa                                                            >  2:5776199/1‑93 (MQ=255)

                             aCGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATaa                                                            >  1:5976347/1‑93 (MQ=255)

                             aCGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATaa                                                            <  1:4954743/93‑1 (MQ=255)

                             aCGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATaa                                                            <  1:1846880/93‑1 (MQ=255)

                                cTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCa                                                         >  2:3876494/1‑93 (MQ=255)

                                 tAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAg                                                        >  1:2011875/1‑93 (MQ=255)

                                  aaaaaTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAgg                                                       <  2:3062021/93‑1 (MQ=255)

                                   aaaaTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGa                                                      <  2:3624741/93‑1 (MQ=255)

                                   aaaaTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGGAATATCAAGACGCAGGCGGGCCATGCTGCGTTCGAACAGTGCGATAACCAGGa                                                      >  2:6309544/1‑93 (MQ=255)

                                    aaaTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGAc                                                     <  1:5813019/93‑1 (MQ=255)

                                      aTGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGc                                                   >  1:4077247/1‑93 (MQ=255)

                                       tGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGcc                                                  <  1:4103717/93‑1 (MQ=255)

                                       tGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGcc                                                  >  1:5070807/1‑93 (MQ=255)

                                        gCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCg                                                 >  2:4389879/1‑93 (MQ=255)

                                         cAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGa                                                >  1:6241939/1‑93 (MQ=255)

                                          aaaGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGac                                               >  2:3135066/1‑93 (MQ=255)

                                          aaaGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGac                                               >  1:3770679/1‑93 (MQ=255)

                                             gCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGaccac                                            >  1:2402643/1‑93 (MQ=255)

                                             gCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGaccac                                            >  1:5442216/1‑93 (MQ=255)

                                                 aaCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGt                                        >  2:4847623/1‑93 (MQ=255)

                                                  aCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGtt                                       >  1:5013217/1‑93 (MQ=255)

                                                  aCCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGtc                                       <  2:6051092/93‑2 (MQ=255)

                                                   cccagcccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGCTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGttt                                      <  2:4217572/93‑1 (MQ=255)

                                                      agcccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTAc                                   >  1:3067329/1‑93 (MQ=255)

                                                       gcccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACTACCAGTTTTACg                                  <  1:3020893/93‑1 (MQ=255)

                                                       gcccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACg                                  >  1:4299091/1‑93 (MQ=255)

                                                        cccagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGa                                 >  1:2152298/1‑93 (MQ=255)

                                                          cagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGCTg                               >  2:2870992/1‑93 (MQ=255)

                                                          cagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATg                               >  1:1005228/1‑93 (MQ=255)

                                                          cagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATg                               >  2:3759222/1‑93 (MQ=255)

                                                          cagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATg                               >  2:1965202/1‑93 (MQ=255)

                                                          cagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATg                               >  2:733237/1‑93 (MQ=255)

                                                          cagGTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGAACACCAGTTTTACGATg                               >  2:2870993/1‑93 (MQ=255)

                                                            ggTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCCTGCTGGTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGc                             >  2:5293597/1‑93 (MQ=255)

                                                            ggTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGc                             >  1:3796235/1‑93 (MQ=255)

                                                            ggTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGc                             >  1:2760997/1‑93 (MQ=255)

                                                            ggTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGc                             <  1:2622406/93‑1 (MQ=255)

                                                             gTAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCa                            >  2:4902536/1‑93 (MQ=255)

                                                              tAATGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCaa                           >  2:1061386/1‑93 (MQ=255)

                                                                aTGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATc                         <  1:1197051/93‑1 (MQ=255)

                                                                aTGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATc                         <  1:1545636/93‑1 (MQ=255)

                                                                 tGCGCGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCa                        <  2:3713137/93‑1 (MQ=255)

                                                                  gcgcGGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCAc                       >  2:425243/1‑93 (MQ=255)

                                                                   cgcgGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCAcc                      <  1:1471864/93‑1 (MQ=255)

                                                                   cgcgGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCAcc                      <  1:3328888/93‑1 (MQ=255)

                                                                     cgGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAg                    <  2:4593439/93‑1 (MQ=255)

                                                                     cgGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAg                    <  1:4377013/93‑1 (MQ=255)

                                                                     cgGAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAg                    <  2:1407348/93‑1 (MQ=255)

                                                                      ggAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAgc                   >  1:697749/1‑93 (MQ=255)

                                                                       gAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAgcg                  >  1:4005828/1‑93 (MQ=255)

                                                                       gAGTAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAgcg                  >  1:4044947/1‑93 (MQ=255)

                                                                          tAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCca               <  2:741637/93‑1 (MQ=255)

                                                                          tAATATCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCca               >  1:2077533/1‑93 (MQ=255)

                                                                             tatCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagca            >  1:801182/1‑93 (MQ=255)

                                                                              atCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagcag           >  1:3064208/1‑93 (MQ=255)

                                                                               tCAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagcagc          <  2:2668636/93‑1 (MQ=255)

                                                                                cAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagcagca         >  2:4472704/1‑93 (MQ=255)

                                                                                cAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagcagca         >  2:3730687/1‑93 (MQ=255)

                                                                                cAAGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagcagca         >  1:938329/1‑93 (MQ=255)

                                                                                 aaGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCcagcagcag        >  1:5246798/1‑93 (MQ=255)

                                                                                  aGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGt       >  2:411798/1‑93 (MQ=255)

                                                                                  aGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGt       <  2:5095424/93‑1 (MQ=255)

                                                                                  aGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGt       >  2:5652461/1‑93 (MQ=255)

                                                                                  aGACGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGt       <  1:5383311/93‑1 (MQ=255)

                                                                                    aCGCAGGCGGTCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTcc     <  2:46287/93‑1 (MQ=255)

                                                                                     cGCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCa    <  1:3000867/93‑1 (MQ=255)

                                                                                      gCAGGCGGGCCATGCTTTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCAc   <  1:1159906/93‑1 (MQ=255)

                                                                                      gCAGGCGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACTATGGCAATCACCAGCGCCAGCAGCAGTCGAc   >  1:234450/1‑93 (MQ=255)

CTTTAATCACGCCGCCAGCAAGGAGACGAACGCTAAAAATGCAAAGCCAAACCCAGCCCAGGTAATGCGCGGAGTAATATCAAGACGCAGACGGGCCATGCTGTTTTCGAACAGCGCGATAACCAGGACGCCGACCACCAGTTTTACGATGGCAATCACCAGCGCCAGCAGCAGTCCACC  >  NC_000913/2844506‑2844685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`