BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,941,864	T→C	M169T (ATG→ACG)	b2810 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,941,864	0	T	C	99.6%	780.6 / ‑4.3	240	M169T (ATG→ACG)	b2810	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (122/117); minor base A (0/1); total (122/118)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.67e-01

Only 100 of 248 total aligned reads displayed.

AATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGC  >  NC_000913/2941774‑2941951

aaTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGt                                                                                       <  1:6670/93‑1 (MQ=255)

aaTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGt                                                                                       <  1:1947145/93‑1 (MQ=255)

aaTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGt                                                                                       >  2:6240664/1‑93 (MQ=255)

 aTTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTc                                                                                      >  1:3787427/1‑93 (MQ=255)

  ttGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCg                                                                                     >  1:926860/1‑93 (MQ=255)

     ccGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAAc                                                                                  >  2:2667862/1‑93 (MQ=255)

     ccGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAAc                                                                                  >  1:3564904/1‑93 (MQ=255)

     ccGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGCTGGGTCAGACGTCGAAc                                                                                  >  2:3425136/1‑93 (MQ=255)

       gCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGt                                                                                >  2:4002774/1‑93 (MQ=255)

       gCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGt                                                                                <  2:4107109/93‑1 (MQ=255)

         ttAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTa                                                                              >  1:1356162/1‑93 (MQ=255)

         ttAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTa                                                                              >  1:2490753/1‑93 (MQ=255)

            aTGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTg                                                                           <  1:892468/93‑1 (MQ=255)

              gcgcAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGc                                                                         <  2:1325250/93‑1 (MQ=255)

                gcAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCgg                                                                       >  2:2124303/1‑93 (MQ=255)

                gcAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCgg                                                                       >  1:5464771/1‑93 (MQ=255)

                 cAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGt                                                                      >  2:4660016/1‑93 (MQ=255)

                  aGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCgggt                                                                     >  2:4368674/1‑91 (MQ=255)

                  aGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGtt                                                                     <  1:801286/93‑1 (MQ=255)

                   gCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTg                                                                    >  1:2823396/1‑93 (MQ=255)

                   gCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTg                                                                    >  2:3553528/1‑93 (MQ=255)

                   gCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTg                                                                    <  2:2167881/93‑1 (MQ=255)

                   gCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTg                                                                    >  2:598709/1‑93 (MQ=255)

                     gACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTATGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGGTggc                                                                  >  1:107988/1‑91 (MQ=255)

                        tGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCgg                                                               <  1:856219/93‑1 (MQ=255)

                         gCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGa                                                              >  2:43804/1‑93 (MQ=255)

                          ccGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGac                                                             >  1:2143459/1‑92 (MQ=255)

                          ccGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGAt                                                             <  1:2580490/93‑1 (MQ=255)

                          ccGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGAt                                                             <  2:4996744/93‑1 (MQ=255)

                          ccGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGAt                                                             <  2:6153742/93‑1 (MQ=255)

                           cGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATc                                                            <  1:2209646/93‑1 (MQ=255)

                           cGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATc                                                            >  2:419596/1‑93 (MQ=255)

                           cGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATc                                                            >  2:4493732/1‑93 (MQ=255)

                           cGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATc                                                            <  1:2587351/93‑1 (MQ=255)

                            ggATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCt                                                           >  1:440952/1‑93 (MQ=255)

                            ggATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCt                                                           >  2:372032/1‑93 (MQ=255)

                             gATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCCGCTCTg                                                          >  2:718757/1‑93 (MQ=255)

                               tGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGgc                                                        <  1:5141717/93‑1 (MQ=255)

                                 tCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGgcgc                                                      <  1:536598/93‑1 (MQ=255)

                                  cGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGcgcg                                                     >  2:2519875/1‑93 (MQ=255)

                                  cGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGcgcg                                                     <  1:5693208/93‑1 (MQ=255)

                                  cGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGGTGCCCGGATCTGGcgcg                                                     >  2:1848393/1‑93 (MQ=255)

                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGcgcgg                                                    >  2:3234938/1‑92 (MQ=255)

                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGcgcgg                                                    >  1:412816/1‑92 (MQ=255)

                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcga                                                    <  1:2051097/93‑1 (MQ=255)

                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcga                                                    <  1:6301777/93‑1 (MQ=255)

                                   gATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcga                                                    >  1:4521301/1‑93 (MQ=255)

                                    aTTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgag                                                   >  2:6280499/1‑93 (MQ=255)

                                     tttGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagc                                                  >  1:4664589/1‑93 (MQ=255)

                                      ttgttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagcg                                                 <  1:4764005/93‑1 (MQ=255)

                                       tgttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCgcgagcga                                                >  2:1737689/1‑93 (MQ=255)

                                        gttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAt                                               >  2:1498446/1‑93 (MQ=255)

                                         ttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAtt                                              <  2:3937666/93‑1 (MQ=255)

                                         ttgCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGAtt                                              >  1:747292/1‑93 (MQ=255)

                                             cAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCt                                          <  2:3673654/93‑1 (MQ=255)

                                             cAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCt                                          >  1:2894077/1‑93 (MQ=255)

                                             cAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCt                                          <  2:5572956/93‑1 (MQ=255)

                                             cAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCt                                          <  2:5391025/93‑1 (MQ=255)

                                              aGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCtt                                         <  1:3849824/93‑1 (MQ=255)

                                              aGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCtt                                         <  1:6013133/93‑1 (MQ=255)

                                              aGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCtt                                         >  1:1865858/1‑93 (MQ=255)

                                                     aTTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCGCGAGCGATTACCTTTGCTCAt                                  >  1:711229/1‑93 (MQ=255)

                                                     aTTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCAt                                  <  1:5854495/93‑1 (MQ=255)

                                                        aCTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCa                               <  2:2023974/93‑1 (MQ=255)

                                                        aCTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCa                               <  1:625423/93‑1 (MQ=255)

                                                          tCCCCGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGc                             >  1:2373178/1‑93 (MQ=255)

                                                           ccccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGcc                            <  2:5298380/93‑1 (MQ=255)

                                                           ccccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGcc                            <  1:2988792/93‑1 (MQ=255)

                                                            cccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCg                           >  2:2403336/1‑93 (MQ=255)

                                                             ccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCgg                          >  1:578442/1‑93 (MQ=255)

                                                             ccGTAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCgg                          <  2:3078144/93‑1 (MQ=255)

                                                                tAGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAt                       <  1:4958620/93‑1 (MQ=255)

                                                                 aGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGGTCTGGCGCGAGCGGTTACCTTTGCTCATTCAGCCGGGGtg                      >  2:2282457/1‑93 (MQ=255)

                                                                 aGTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtg                      <  1:3257220/93‑1 (MQ=255)

                                                                  gTCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGCtgg                     >  1:3519221/1‑93 (MQ=255)

                                                                   tCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggt                    <  2:514587/93‑1 (MQ=255)

                                                                   tCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggt                    <  2:620984/93‑1 (MQ=255)

                                                                   tCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggg                    >  1:4831927/1‑92 (MQ=255)

                                                                   tCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggg                    >  1:1670741/1‑92 (MQ=255)

                                                                   tCGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggg                    >  2:1599009/1‑92 (MQ=255)

                                                                    cGGATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtg                   <  2:425446/93‑1 (MQ=255)

                                                                     ggATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtggtgg                  >  2:1469538/1‑93 (MQ=255)

                                                                      gATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGAtgggggg                 >  2:2974883/1‑92 (MQ=255)

                                                                      gATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATggtggt                 <  2:924068/93‑1 (MQ=255)

                                                                      gATTCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATggtggt                 >  1:5637183/1‑93 (MQ=255)

                                                                        ttCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtga               >  1:3614599/1‑93 (MQ=255)

                                                                        ttCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGGTGTCCGGATCCGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGGtggtggggg               >  1:4305645/1‑90 (MQ=255)

                                                                        ttCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGGTGGCCGGCTCTGGCGCGAGCGATTACCTCTGCTCCTTCAGCCGGGATGGTGGtga               >  2:3017362/1‑93 (MQ=255)

                                                                         tCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgat              <  1:130191/93‑1 (MQ=255)

                                                                         tCTGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgat              <  2:772233/93‑1 (MQ=255)

                                                                           tGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatgg            <  1:2729361/93‑1 (MQ=255)

                                                                           tGGCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatgg            >  1:1057274/1‑93 (MQ=255)

                                                                            ggCGTTGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggt           <  2:5795192/93‑1 (MQ=255)

                                                                                ttGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgat       <  1:338725/93‑1 (MQ=255)

                                                                                ttGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgat       >  2:872457/1‑93 (MQ=255)

                                                                                ttGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgat       <  1:1001880/93‑1 (MQ=255)

                                                                                gtGGGTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgat       <  1:584222/92‑1 (MQ=255)

                                                                                  gggTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGtgatggttgatgg     <  2:6030239/93‑1 (MQ=255)

                                                                                    gTCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTg   >  2:1265580/1‑93 (MQ=255)

                                                                                     tCAGACGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGGGGTGATGGTTGATGGTGc  >  1:3247013/1‑93 (MQ=255)

AATTGCCGCTTAATGCGCAGCGACTGCCGGATGTCGATTTGTTGCCAGAACTGATTACTCCCCGTAGTCGGATTCTGGCGTTGGGTCAGATGTCGAACGTTACTGGCGGTTGCCCGGATCTGGCGCGAGCGATTACCTTTGCTCATTCAGCCGGGATGGTGGTGATGGTTGATGGTGC  >  NC_000913/2941774‑2941951

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`