BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,219	T→C	H59H (CAT→CAC)	lysR →	LysR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,219	0	T	C	99.6%	719.9 / ‑4.8	223	H59H (CAT→CAC)	lysR	LysR
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (92/130); total (93/130)
Fisher's exact test for biased strand distribution p-value = 4.17e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.58e-01

Only 100 of 228 total aligned reads displayed.

ACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGC  >  NC_000913/2977130‑2977309

  cTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAc                                                                                       <  2:3397941/93‑1 (MQ=255)

  cTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAc                                                                                       >  1:2009618/1‑93 (MQ=255)

  cTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAc                                                                                       <  2:3155720/93‑1 (MQ=255)

   tCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAcc                                                                                      >  1:4655846/1‑93 (MQ=255)

   tCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTAcc                                                                                      <  2:2768901/93‑1 (MQ=255)

          cAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAg                                                                               <  2:2061411/93‑1 (MQ=255)

          cAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAg                                                                               <  2:113557/93‑1 (MQ=255)

          cAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAg                                                                               <  2:5541244/93‑1 (MQ=255)

           aaCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAgg                                                                              >  2:5809451/1‑93 (MQ=255)

           aaCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAgg                                                                              >  1:481199/1‑93 (MQ=255)

           aaCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAgg                                                                              >  1:3242213/1‑93 (MQ=255)

            aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGa                                                                             >  2:3732444/1‑93 (MQ=255)

            aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGa                                                                             <  1:5183038/93‑1 (MQ=255)

            aCCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGa                                                                             >  2:1975579/1‑93 (MQ=255)

              cGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACt                                                                           <  2:3479838/93‑1 (MQ=255)

               gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTg                                                                          <  2:5334000/93‑1 (MQ=255)

               gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTg                                                                          <  1:3218521/93‑1 (MQ=255)

               gTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTg                                                                          <  2:4842492/93‑1 (MQ=255)

                tCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGc                                                                         <  1:4042439/93‑1 (MQ=255)

                  aGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGt                                                                       >  2:2901590/1‑93 (MQ=255)

                   gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTc                                                                      <  2:4769696/93‑1 (MQ=255)

                   gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTc                                                                      >  2:3789394/1‑93 (MQ=255)

                   gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTc                                                                      <  2:2555004/93‑1 (MQ=255)

                   gCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCATGCAAGGACTGCGTc                                                                      <  1:412193/93‑1 (MQ=255)

                     cgcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTg                                                                    >  1:4218971/1‑93 (MQ=255)

                      gcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGt                                                                   <  2:1481384/93‑1 (MQ=255)

                      gcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGt                                                                   <  2:5068122/93‑1 (MQ=255)

                      gcgAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGt                                                                   >  2:831525/1‑93 (MQ=255)

                        gAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTTTTTGAGCGCGTACGTGGGCGATTACACCCTACCGGGCAAGGACTGCGTCTGttt                                                                 <  2:5387869/93‑1 (MQ=255)

                        gAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGttt                                                                 >  1:3697097/1‑93 (MQ=255)

                           cTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaa                                                              >  1:5409535/1‑93 (MQ=255)

                           cTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaa                                                              >  1:5952118/1‑93 (MQ=255)

                            ttGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaag                                                             <  2:4686728/93‑1 (MQ=255)

                             tGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaaga                                                            >  1:4539597/1‑93 (MQ=255)

                             tGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTgaaga                                                            <  1:985447/93‑1 (MQ=255)

                               cgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGaagaag                                                          <  1:5786123/93‑1 (MQ=255)

                               cgcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGCACTGCGTCTGTTTGaagaag                                                          <  2:6064910/93‑1 (MQ=255)

                                gcgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGt                                                         >  2:5262254/1‑93 (MQ=255)

                                 cgcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTg                                                        <  2:4384826/93‑1 (MQ=255)

                                  gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGc                                                       <  2:1863876/93‑1 (MQ=255)

                                  gcTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGc                                                       >  1:6012831/1‑93 (MQ=255)

                                   cTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCa                                                      <  1:2307380/93‑1 (MQ=255)

                                    tttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCaa                                                     <  2:4332610/93‑1 (MQ=255)

                                     ttGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAAc                                                    <  2:3358331/93‑1 (MQ=255)

                                       gagaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGa                                                  >  1:33494/1‑93 (MQ=255)

                                       gagaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGa                                                  <  2:2489633/93‑1 (MQ=255)

                                         gaAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATc                                                >  2:2440870/1‑93 (MQ=255)

                                          aaGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATcc                                               <  1:5004418/93‑1 (MQ=255)

                                           aGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCt                                              <  2:107291/93‑1 (MQ=255)

                                              tGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGt                                           <  2:6093136/93‑1 (MQ=255)

                                               gATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGGAACGATCCTGGTa                                          >  2:1617741/1‑93 (MQ=255)

                                               gATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTa                                          <  1:2359631/93‑1 (MQ=255)

                                                aTCGGGCTGAAATTGTTTGAGCTCGTACGTGGGCGATTACGCCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAATTGCAACGATCCTGGTAc                                         <  2:4878776/93‑1 (MQ=255)

                                                aTCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTAc                                         >  2:1689898/1‑93 (MQ=255)

                                                aTCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTAc                                         >  1:2831835/1‑93 (MQ=255)

                                                 tCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACg                                        <  2:1248638/93‑1 (MQ=255)

                                                    ggCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGAc                                     <  2:4470804/93‑1 (MQ=255)

                                                     gCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACt                                    <  1:1024915/93‑1 (MQ=255)

                                                        gAAATTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGa                                 >  1:5663343/1‑93 (MQ=255)

                                                         aaaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGAt                                >  1:5859667/1‑93 (MQ=255)

                                                         aaaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACAATCCTGGTACTGACTGGAt                                <  2:6107891/93‑1 (MQ=255)

                                                          aaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATc                               <  2:5514529/93‑1 (MQ=255)

                                                          aaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATc                               <  2:3619192/93‑1 (MQ=255)

                                                          aaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATc                               <  1:4619917/93‑1 (MQ=255)

                                                          aaTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATc                               >  1:5223826/1‑93 (MQ=255)

                                                           aTTGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCg                              >  2:4629341/1‑93 (MQ=255)

                                                             tGTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCa                            <  2:4055920/93‑1 (MQ=255)

                                                              gTTTGAGCGCGTACGTGGGCGATTACCCCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                           <  1:4309468/93‑1 (MQ=255)

                                                              gTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                           <  1:4688504/93‑1 (MQ=255)

                                                              gTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                           >  1:513709/1‑93 (MQ=255)

                                                              gTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                           <  2:6264491/93‑1 (MQ=255)

                                                              gTTTGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAt                           <  2:4726184/93‑1 (MQ=255)

                                                               tttGAGCGCGTACGTGGGCGATTACGCCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAtt                          <  2:950550/93‑1 (MQ=255)

                                                               tttGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCAtt                          <  2:804385/93‑1 (MQ=255)

                                                                 tGAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGt                        <  1:1460638/93‑1 (MQ=255)

                                                                  gAGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTc                       <  2:1794303/93‑1 (MQ=255)

                                                                   aGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCa                      <  2:5702544/93‑1 (MQ=255)

                                                                   aGCGCGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTAGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCa                      >  2:6213215/1‑93 (MQ=255)

                                                                    gcgcGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAg                     >  1:2135378/1‑93 (MQ=255)

                                                                    gcgcGTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAg                     <  1:2404981/93‑1 (MQ=255)

                                                                     cgcgTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAgc                    <  1:1597887/93‑1 (MQ=255)

                                                                        ttACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgcc                 <  1:297659/92‑1 (MQ=255)

                                                                        gTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgcc                 >  1:6136017/1‑93 (MQ=255)

                                                                        gTACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgcc                 >  1:6217569/1‑93 (MQ=255)

                                                                        gTACGTGGGAGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgcc                 >  1:4078908/1‑93 (MQ=255)

                                                                         tACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgccg                >  2:5960868/1‑93 (MQ=255)

                                                                         tACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgccg                >  2:1759713/1‑93 (MQ=255)

                                                                         tACGTGGGCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGcgccg                >  1:1357434/1‑93 (MQ=255)

                                                                           cGTGGGCGATTTCACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTACAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCa              <  2:1479652/93‑1 (MQ=255)

                                                                              gggCGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGaa           <  1:448156/93‑1 (MQ=255)

                                                                                 cGATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGt        <  2:5007797/93‑1 (MQ=255)

                                                                                  gATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTc       <  1:3607186/93‑1 (MQ=255)

                                                                                  gATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTc       <  2:6240018/93‑1 (MQ=255)

                                                                                  gATTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTc       <  2:2074229/93‑1 (MQ=255)

                                                                                   aTTACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCt      >  1:1700628/1‑93 (MQ=255)

                                                                                    ttACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTg     >  1:1889655/1‑93 (MQ=255)

                                                                                    ttACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTg     <  1:6165707/93‑1 (MQ=255)

                                                                                     tACACCCTCCCGTGCAAGTACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGTACTGGATTGCATTGTCACCGCCGCAGAAAGTCTgc    <  2:3042772/93‑1 (MQ=255)

                                                                                     tACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTgc    <  1:547144/93‑1 (MQ=255)

                                                                                     tACACCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTgc    >  2:199938/1‑93 (MQ=255)

ACCTCACAGCCAACCGTCAGCCGCGAACTTGCGCGCTTTGAGAAGGTGATCGGGCTGAAATTGTTTGAGCGCGTACGTGGGCGATTACATCCTACCGTGCAAGGACTGCGTCTGTTTGAAGAAGTGCAACGATCCTGGTACGGACTGGATCGCATTGTCAGCGCCGCAGAAAGTCTGCGC  >  NC_000913/2977130‑2977309

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`