BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,977,707	T→C	V222A (GTA→GCA)	lysR →	LysR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,977,707	0	T	C	99.5%	661.0 / ‑4.5	206	V222A (GTA→GCA)	lysR	LysR
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (114/91); total (115/91)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.89e-01

Only 100 of 209 total aligned reads displayed.

GAACTACATCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGTAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGCACTAGATTATG  >  NC_000913/2977618‑2977796

gaACTACATCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGa                                                                                        >  1:1002249/1‑93 (MQ=255)

 aaCTACATCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGaa                                                                                       >  2:1139272/1‑93 (MQ=255)

 aaCTACATCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGaa                                                                                       <  2:3360436/93‑1 (MQ=255)

  aCTACATCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGaaa                                                                                      <  2:6153530/93‑1 (MQ=255)

   cTACATCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAAc                                                                                     <  2:3226287/93‑1 (MQ=255)

   cTACATCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGGTAAACGACGCATGATCGCAGAAAc                                                                                     >  2:2522850/1‑93 (MQ=255)

    tACATCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAAcc                                                                                    >  1:4917520/1‑93 (MQ=255)

    tACATCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTAATTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAAcc                                                                                    <  1:829104/93‑1 (MQ=255)

      cATCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCca                                                                                  <  1:1670887/93‑1 (MQ=255)

       aTCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCcac                                                                                 <  2:426487/93‑1 (MQ=255)

       aTCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCcac                                                                                 <  1:4095120/93‑1 (MQ=255)

        tCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCcaca                                                                                >  1:79413/1‑93 (MQ=255)

         cAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAg                                                                               <  1:4507243/93‑1 (MQ=255)

         cAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAg                                                                               <  2:4620316/93‑1 (MQ=255)

          aGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAgc                                                                              >  2:3622752/1‑93 (MQ=255)

            ccTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAgcgc                                                                            <  1:2352630/93‑1 (MQ=255)

             cTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGcgcc                                                                           >  2:6209551/1‑93 (MQ=255)

              tttCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGcgccg                                                                          >  2:353940/1‑93 (MQ=255)

                 cccGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGt                                                                       >  2:3020617/1‑93 (MQ=255)

                 cccGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATGTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGt                                                                       <  2:3197528/93‑1 (MQ=255)

                   cGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTTAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGGCa                                                                     >  2:2129819/1‑93 (MQ=255)

                   cGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCa                                                                     <  1:2526417/93‑1 (MQ=255)

                     tACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGt                                                                   <  1:1292485/93‑1 (MQ=255)

                     tACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGt                                                                   >  1:1504104/1‑93 (MQ=255)

                      aCTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTc                                                                  <  2:3784313/93‑1 (MQ=255)

                       cttaCAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCt                                                                 <  1:1934911/90‑1 (MQ=255)

                       cTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCt                                                                 >  1:2654807/1‑93 (MQ=255)

                       cTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCt                                                                 >  1:4943168/1‑93 (MQ=255)

                           cAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTgcgc                                                             >  1:3481423/1‑93 (MQ=255)

                           cAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTgcgc                                                             >  2:6139504/1‑93 (MQ=255)

                             gCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCaa                                                           <  2:2876812/93‑1 (MQ=255)

                             gCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCaa                                                           <  1:1387575/93‑1 (MQ=255)

                               tATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATg                                                         >  2:3833342/1‑93 (MQ=255)

                                aTCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATgg                                                        >  2:277020/1‑93 (MQ=255)

                                  cGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTa                                                      <  2:4439295/93‑1 (MQ=255)

                                   gCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAc                                                     <  1:3042884/93‑1 (MQ=255)

                                    ccAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcg                                                    >  2:2683565/1‑93 (MQ=255)

                                    ccAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcg                                                    <  1:1549101/93‑1 (MQ=255)

                                    ccAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcg                                                    >  1:5088010/1‑93 (MQ=255)

                                    ccAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcg                                                    >  1:3753500/1‑93 (MQ=255)

                                     cAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcgg                                                   <  1:5863048/93‑1 (MQ=255)

                                      aGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcggg                                                  <  1:6104609/93‑1 (MQ=255)

                                      aGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcggg                                                  <  2:4859786/93‑1 (MQ=255)

                                      aGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcggg                                                  <  1:3982531/93‑1 (MQ=255)

                                       gTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcgggg                                                 >  2:3985492/1‑92 (MQ=255)

                                       gTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcgggc                                                 >  1:5879249/1‑93 (MQ=255)

                                       gTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcgggc                                                 >  1:2134352/1‑93 (MQ=255)

                                       gTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcgggc                                                 <  1:219648/93‑1 (MQ=255)

                                        ttGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcgggcg                                                <  2:5940549/93‑1 (MQ=255)

                                        ttGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcgggcg                                                <  1:2400005/93‑1 (MQ=255)

                                          gCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTAcgggcggg                                              <  2:3292531/93‑1 (MQ=255)

                                            tGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAACCCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCggggg                                            <  1:4834724/93‑1 (MQ=255)

                                              gATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGggta                                          <  1:5320224/93‑1 (MQ=255)

                                                tCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGggtagg                                        >  1:991535/1‑93 (MQ=255)

                                                tCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGggtagc                                        >  1:4144225/1‑92 (MQ=255)

                                                tCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGgggagg                                        >  2:1932966/1‑93 (MQ=255)

                                                  aGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGggtaggta                                      >  2:4618043/1‑93 (MQ=255)

                                                   gCTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTAt                                     >  2:1307824/1‑93 (MQ=255)

                                                    cTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTAtt                                    >  2:432967/1‑93 (MQ=255)

                                                    cTATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGGAGGTAtt                                    >  1:6070447/1‑93 (MQ=255)

                                                     tATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTCGGTAttt                                   >  2:5416587/1‑93 (MQ=255)

                                                     tATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTAttt                                   >  2:4451839/1‑93 (MQ=255)

                                                     tATTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTAttt                                   <  1:5183960/93‑1 (MQ=255)

                                                      aTTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTc                                  <  2:3659927/93‑1 (MQ=255)

                                                      aTTTACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGCATTTc                                  >  1:3015512/1‑93 (MQ=255)

                                                       tttACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCg                                 >  2:5803342/1‑93 (MQ=255)

                                                        ttACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCgg                                <  2:5640479/93‑1 (MQ=255)

                                                        ttACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCgg                                >  2:3715197/1‑93 (MQ=255)

                                                         tACTGAACATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCggt                               >  2:5820674/1‑93 (MQ=255)

                                                              aaCATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTa                          >  2:3095709/1‑93 (MQ=255)

                                                              aaCATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTa                          <  1:3165083/93‑1 (MQ=255)

                                                               aCATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTaa                         >  2:1094532/1‑93 (MQ=255)

                                                                cATCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAAc                        <  1:2836414/93‑1 (MQ=255)

                                                                 aTCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAAcc                       >  2:3903625/1‑93 (MQ=255)

                                                                  tCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAAccc                      >  1:554137/1‑93 (MQ=255)

                                                                  tCAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAAccc                      <  2:3963156/93‑1 (MQ=255)

                                                                   cAGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCg                     >  2:1170977/1‑93 (MQ=255)

                                                                    aGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGc                    >  2:480515/1‑93 (MQ=255)

                                                                    aGGTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGc                    <  2:736826/93‑1 (MQ=255)

                                                                      gTTAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGCATTTCGGTGGTTAACCCGCTc                  >  1:6167394/1‑93 (MQ=255)

                                                                        tAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCAc                >  2:2564884/1‑93 (MQ=255)

                                                                        tAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCAc                <  1:4010801/93‑1 (MQ=255)

                                                                        tAAACGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCAc                >  2:1228574/1‑93 (MQ=255)

                                                                         aaaCGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCAcc               <  2:4489352/93‑1 (MQ=255)

                                                                         aaaCGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCAcc               >  2:5172719/1‑93 (MQ=255)

                                                                         aaaCGACGCATGATCGCAGAAACCCACAGCGCCGAGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCAcc               <  1:4762360/93‑1 (MQ=255)

                                                                          aaCGACGCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCg              >  1:2936687/1‑93 (MQ=255)

                                                                           acgacgCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGc             >  1:1810088/1‑93 (MQ=255)

                                                                              acgCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGCACt          >  2:5055557/1‑93 (MQ=255)

                                                                              acgCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGCACt          >  2:1622721/1‑93 (MQ=255)

                                                                               cgCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGCACTa         <  2:5786354/93‑1 (MQ=255)

                                                                                gCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGCACTAg        >  1:3279333/1‑93 (MQ=255)

                                                                                gCATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATAGTACGGGCGGGGGAAGGTATTTCGGTGGTTAACCCGCTCACCGCACTAg        >  1:5705990/1‑93 (MQ=255)

                                                                                 cATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGCACTAGa       >  2:4536128/1‑93 (MQ=255)

                                                                                 cATGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGCACTAGa       >  2:6097860/1‑93 (MQ=255)

                                                                                   tGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGCACTAGAtt     >  2:5247976/1‑93 (MQ=255)

                                                                                   tGATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGCACTAGAtt     >  2:1432670/1‑93 (MQ=255)

                                                                                    gATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGCACTAGATTa    <  1:855996/93‑1 (MQ=255)

                                                                                    gATCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGCACTAGATTa    <  2:1334757/93‑1 (MQ=255)

                                                                                      tCGCAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGCACTAGATTATg  >  1:3820085/1‑93 (MQ=255)

GAACTACATCAGCCTTTCCCGTACTGACAGCTATCGCCAGTTGCTGGATCAGCTATTTACTGAACATCAGGTTAAACGACGCATGATCGTAGAAACCCACAGCGCCGCGTCAGTCTGCGCAATGGTACGGGCGGGGGTAGGTATTTCGGTGGTTAACCCGCTCACCGCACTAGATTATG  >  NC_000913/2977618‑2977796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`