BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,026,063	A→G	D129G (GAC→GGC)	ygfR →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,026,063	0	A	G	99.6%	763.5 / NA	233	D129G (GAC→GGC)	ygfR	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (98/134); minor base C (1/0); total (99/134)
Fisher's exact test for biased strand distribution p-value = 4.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 236 total aligned reads displayed.

CTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCGGGGGTA  >  NC_000913/3025973‑3026152

 tACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTc                                                                                        >  1:5611028/1‑93 (MQ=255)

 tACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTc                                                                                        >  2:5171480/1‑93 (MQ=255)

  aCTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCa                                                                                       <  1:4521493/93‑1 (MQ=255)

  aCTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCa                                                                                       <  1:2106344/93‑1 (MQ=255)

   cTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAg                                                                                      >  1:4300002/1‑93 (MQ=255)

      gCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTaa                                                                                   >  2:5333294/1‑93 (MQ=255)

      gCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTaa                                                                                   >  1:1958546/1‑93 (MQ=255)

       cACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAg                                                                                  <  2:4605871/93‑1 (MQ=255)

        aCCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGt                                                                                 >  1:3216370/1‑93 (MQ=255)

        aCCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGt                                                                                 <  2:334934/93‑1 (MQ=255)

          cATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTc                                                                               >  1:5754260/1‑93 (MQ=255)

          cATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTc                                                                               <  1:4075339/93‑1 (MQ=255)

            tCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCaa                                                                             <  1:4851660/93‑1 (MQ=255)

              cGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAata                                                                           <  2:1591505/93‑1 (MQ=255)

              cGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAata                                                                           <  2:2330857/93‑1 (MQ=255)

               gTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAatat                                                                          >  2:4816000/1‑93 (MQ=255)

               gTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAatat                                                                          <  1:5366107/93‑1 (MQ=255)

               gTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAatat                                                                          <  1:1278131/93‑1 (MQ=255)

               gTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAatat                                                                          >  2:305797/1‑93 (MQ=255)

               gTGCCGTCGCCGGTCAGGCAAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAatat                                                                          >  2:4789620/1‑93 (MQ=255)

                tGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGGTCAATAtt                                                                         >  1:5410670/1‑93 (MQ=255)

                 gCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATtc                                                                        >  1:3745627/1‑93 (MQ=255)

                 gCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATtc                                                                        <  1:171554/93‑1 (MQ=255)

                    gttgCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTcc                                                                     >  2:1689400/4‑93 (MQ=255)

                     tCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCg                                                                    <  1:5238454/93‑1 (MQ=255)

                        ccGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGcc                                                                 >  2:3261889/1‑93 (MQ=255)

                         cGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCt                                                                <  1:5714506/93‑1 (MQ=255)

                          ggTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCtg                                                               >  1:887637/1‑93 (MQ=255)

                          ggTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCtg                                                               >  1:1139686/1‑93 (MQ=255)

                           gTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGATCAATATTCTCCGGCCtgg                                                              >  1:3209582/1‑93 (MQ=255)

                            tCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCtggt                                                             <  2:242178/93‑1 (MQ=255)

                            tCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCtggt                                                             >  2:3677426/1‑93 (MQ=255)

                             taGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCtggtg                                                            <  2:3896485/92‑1 (MQ=255)

                             cAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCtggtg                                                            <  2:3510931/93‑1 (MQ=255)

                              aGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCtggtgg                                                           >  2:2801578/1‑93 (MQ=255)

                                gCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGgc                                                         >  2:2177780/1‑93 (MQ=255)

                                gCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGgc                                                         <  2:3170235/93‑1 (MQ=255)

                                  gAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGgcgc                                                       >  2:1867974/1‑93 (MQ=255)

                                   aaTTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCa                                                      >  1:694822/1‑93 (MQ=255)

                                   aaTTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCa                                                      <  1:3291766/93‑1 (MQ=255)

                                   aaTTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCa                                                      >  2:4234612/1‑93 (MQ=255)

                                    aTTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAg                                                     >  1:599636/1‑93 (MQ=255)

                                    aTTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAg                                                     >  2:3221616/1‑93 (MQ=255)

                                    aTTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAg                                                     <  2:756733/93‑1 (MQ=255)

                                     tttGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAgc                                                    <  2:844832/93‑1 (MQ=255)

                                       tgttgGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAgcgc                                                  >  1:2376960/1‑93 (MQ=255)

                                        gttgGATAAAGACAACCAGATCATCAACAGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGcc                                                 <  1:1006044/93‑1 (MQ=255)

                                         ttgGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGccc                                                <  1:5905604/93‑1 (MQ=255)

                                          tgGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCg                                               <  1:4684897/93‑1 (MQ=255)

                                           ggATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGCCTCAGTAAGGTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGc                                              >  1:4266507/1‑93 (MQ=255)

                                             aTAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAg                                            >  1:3795474/1‑93 (MQ=255)

                                              aaaaGAAAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGc                                           <  1:3073940/92‑1 (MQ=255)

                                                   aCAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGttt                                      <  1:2141509/93‑1 (MQ=255)

                                                   aCAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGttt                                      <  2:5486064/93‑1 (MQ=255)

                                                      aCCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTtata                                   <  2:5070952/93‑1 (MQ=255)

                                                      aCCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTtata                                   <  1:41269/93‑1 (MQ=255)

                                                        cAGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATc                                 <  1:2905732/93‑1 (MQ=255)

                                                         aGATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCg                                >  2:466585/1‑93 (MQ=255)

                                                          gATCATCAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGa                               >  2:2684168/1‑93 (MQ=255)

                                                          gATCATAAACGGCGGCAAAGACATGACCAGTGGCTCTGTAAGGTCAATATTCTCCGGCCTGGTGGGAGCAGCGCCCGCACCGGTTTTTCTCGa                               >  2:1119504/1‑93 (MQ=255)

                                                           atcatcAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGaa                              <  2:2251235/93‑1 (MQ=255)

                                                           atcatcAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGaa                              <  2:3625352/93‑1 (MQ=255)

                                                           atcatcAACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGaa                              <  1:1821981/93‑1 (MQ=255)

                                                            tcatcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAAt                             <  1:1095280/93‑1 (MQ=255)

                                                            tcatcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAAt                             >  1:1796168/1‑93 (MQ=255)

                                                            tcatcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAAt                             >  1:5094212/1‑93 (MQ=255)

                                                            tcatcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAAt                             <  2:1426660/93‑1 (MQ=255)

                                                             catcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATc                            >  1:2748237/1‑93 (MQ=255)

                                                              atcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCa                           <  2:3856248/93‑1 (MQ=255)

                                                              atcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCa                           <  2:4710534/93‑1 (MQ=255)

                                                              atcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCa                           >  2:6227722/1‑93 (MQ=255)

                                                               tcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAg                          <  1:663570/93‑1 (MQ=255)

                                                               tcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAg                          >  2:2902235/1‑93 (MQ=255)

                                                               tcaACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAg                          >  1:2336484/1‑93 (MQ=255)

                                                                caACGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGc                         <  2:2780339/93‑1 (MQ=255)

                                                                 aaCGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCg                        <  2:162872/93‑1 (MQ=255)

                                                                  aCGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCgg                       >  1:746005/1‑93 (MQ=255)

                                                                  aCGGCGGCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCgg                       <  2:1432475/93‑1 (MQ=255)

                                                                   cggcggCAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGc                      <  2:5309639/93‑1 (MQ=255)

                                                                    ggcggcAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCa                     <  1:3727015/93‑1 (MQ=255)

                                                                    ggcggcAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCa                     <  1:1443178/93‑1 (MQ=255)

                                                                       ggcAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGa                  <  2:2474283/93‑1 (MQ=255)

                                                                        gcAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGaa                 >  1:3031049/1‑93 (MQ=255)

                                                                        gcAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGaa                 <  1:6127023/93‑1 (MQ=255)

                                                                        gcAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGaa                 <  1:4946848/93‑1 (MQ=255)

                                                                         cAAAGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAc                <  2:6203879/93‑1 (MQ=255)

                                                                          aaaGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAcc               >  1:4378580/1‑93 (MQ=255)

                                                                           aaGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAccg              >  2:5973551/1‑93 (MQ=255)

                                                                            aGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAccgc             <  2:1372110/93‑1 (MQ=255)

                                                                            aGCCCTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAccgc             <  2:3401313/93‑1 (MQ=255)

                                                                                cTGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAccgccccc         >  2:5674284/1‑93 (MQ=255)

                                                                                cTGACCAGTGGCTCAGTAAGTTCAATATGCTCCGGCCGGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAccgccgcc         <  1:2654214/93‑1 (MQ=255)

                                                                                 tGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAccgccgccg        <  1:149022/93‑1 (MQ=255)

                                                                                 tGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAccgccgccg        <  1:3575527/93‑1 (MQ=255)

                                                                                 tGACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAAccgccgccg        <  2:1187103/93‑1 (MQ=255)

                                                                                  gACCAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAAACAGCGGCAGGAACCGCCGCCgg       <  1:362015/93‑1 (MQ=255)

                                                                                    ccAGTGGCTCAGTAAGTTCAATATTCTCCGGCGTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCgggg     <  2:5775331/93‑1 (MQ=255)

                                                                                    ccAGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCgggg     <  2:3142948/93‑1 (MQ=255)

                                                                                      aGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCgggggg   >  2:6123982/1‑92 (MQ=255)

                                                                                      aGTGGCTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCGGGGGt   <  1:2684168/93‑1 (MQ=255)

CTACTGGCACCATCCGTGCCGTCGCCGGTCAGGCGAATTTGTTGGATAAAGACAACCAGATCATCAACGGCGGCAAAGCCCTGACCAGTGACTCAGTAAGTTCAATATTCTCCGGCCTGGTGGGCGCAGCGCCCGCAGCGGTTTATATCGAATCAGCGGCAGGAACCGCCGCCGGGGGTA  >  NC_000913/3025973‑3026152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`