BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,036,938	T→C	T276A (ACG→GCG)	xerD ←	XerD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,036,938	0	T	C	99.5%	687.4 / ‑4.8	219	T276A (ACG→GCG)	xerD	XerD
Reads supporting (aligned to +/- strand): ref base T (0/1); new base C (105/113); total (105/114)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.53e-01

Only 100 of 245 total aligned reads displayed.

TTCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACG  >  NC_000913/3036848‑3037025

ttCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGCTGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCgcgg                                                                                       <  2:6215943/93‑1 (MQ=255)

ttCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCgcgg                                                                                       >  1:1166137/1‑93 (MQ=255)

ttCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCgcgg                                                                                       >  2:5908244/1‑93 (MQ=255)

ttCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCgcgg                                                                                       <  2:4613250/93‑1 (MQ=255)

 tCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCgcggg                                                                                      >  1:4469498/1‑92 (MQ=255)

   ccGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGcggtgg                                                                                    >  1:506451/1‑93 (MQ=255)

   ccGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGcggtgg                                                                                    >  2:2510641/1‑93 (MQ=255)

   ccGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGcggtgg                                                                                    <  2:2730456/93‑1 (MQ=255)

   ccGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGcggtgg                                                                                    >  2:5563023/1‑93 (MQ=255)

    cGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGga                                                                                   >  1:3485728/1‑93 (MQ=255)

    cGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGga                                                                                   >  2:3149860/1‑93 (MQ=255)

     gTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGgag                                                                                  >  1:683520/1‑93 (MQ=255)

     gTTCTTTTCAGACAGCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGgag                                                                                  >  2:3851791/1‑93 (MQ=255)

        cTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGgagaga                                                                               >  2:168470/1‑93 (MQ=255)

         ctttCAGACCTCACGGCCGCGGGTGGTTCTTTTGATGAAGTTTCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGAt                                                                              <  2:1030804/92‑1 (MQ=255)

          tttCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATc                                                                             <  1:1921455/93‑1 (MQ=255)

          tttCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATc                                                                             >  1:1611195/1‑93 (MQ=255)

           ttCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCg                                                                            <  2:2815165/93‑1 (MQ=255)

            tCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGc                                                                           >  2:2352790/1‑93 (MQ=255)

             cAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCt                                                                          <  2:3078187/93‑1 (MQ=255)

             cAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCt                                                                          <  1:3636607/93‑1 (MQ=255)

              aGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCtg                                                                         >  1:2136197/1‑93 (MQ=255)

              aGACATCACGCCCGCGGGGGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGCCATGCGTATAAATTTGCGCGGTGGAGAGATCGCtg                                                                         >  2:2272963/1‑93 (MQ=255)

               gACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCtgt                                                                        >  2:3465451/1‑93 (MQ=255)

               gACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCtgt                                                                        <  1:2689392/93‑1 (MQ=255)

                aCATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCtgtg                                                                       >  1:5456870/1‑93 (MQ=255)

                   tCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGcc                                                                    >  1:3874336/1‑93 (MQ=255)

                   tCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGcc                                                                    <  1:2413478/93‑1 (MQ=255)

                     aCGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCa                                                                  <  2:5355187/93‑1 (MQ=255)

                      cGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAg                                                                 >  1:2009813/1‑93 (MQ=255)

                          cgcgGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGc                                                             <  2:1230580/93‑1 (MQ=255)

                          cgcgGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGc                                                             <  2:5073072/93‑1 (MQ=255)

                            cgGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCAt                                                           >  2:830242/1‑93 (MQ=255)

                            cgGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCAt                                                           >  1:3722867/1‑93 (MQ=255)

                             gggTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATc                                                          >  2:37628/1‑93 (MQ=255)

                             gggTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATc                                                          <  2:2030103/93‑1 (MQ=255)

                             gggTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATc                                                          <  1:6225606/93‑1 (MQ=255)

                             gggTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATc                                                          <  2:6093604/93‑1 (MQ=255)

                              ggTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCt                                                         >  2:1935170/1‑93 (MQ=255)

                               gTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTg                                                        >  1:516442/1‑93 (MQ=255)

                                   tGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGcacc                                                    >  1:4562777/1‑93 (MQ=255)

                                   tGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGcacc                                                    >  2:3132582/1‑93 (MQ=255)

                                   tGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGcacc                                                    >  2:4319623/1‑93 (MQ=255)

                                   tGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGcacc                                                    >  1:3022435/1‑93 (MQ=255)

                                     cTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGcaccac                                                  >  1:4366464/1‑93 (MQ=255)

                                     cTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGcaccac                                                  <  1:1704257/93‑1 (MQ=255)

                                      tGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCAcg                                                 >  1:5975406/1‑93 (MQ=255)

                                      tGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCAcg                                                 <  2:1343402/93‑1 (MQ=255)

                                      tGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCAcg                                                 <  1:1736339/93‑1 (MQ=255)

                                       gTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCAcgc                                                <  2:4511976/93‑1 (MQ=255)

                                            tgaAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTaaa                                           <  1:1379048/93‑1 (MQ=255)

                                            tgaAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTaaa                                           <  2:3307272/93‑1 (MQ=255)

                                             gaAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAAt                                          <  1:5997139/93‑1 (MQ=255)

                                             gaAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAAt                                          >  2:4379240/1‑93 (MQ=255)

                                              aaGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATc                                         <  1:4617290/93‑1 (MQ=255)

                                               aGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCGTCTGCACCACGCGTAAATcc                                        <  2:3589300/93‑1 (MQ=255)

                                                gTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCg                                       <  2:5107704/93‑1 (MQ=255)

                                                 ttGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGc                                      <  2:3738165/93‑1 (MQ=255)

                                                 ttGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGc                                      >  2:2197342/1‑93 (MQ=255)

                                                      gCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCAt                                 <  1:2199755/93‑1 (MQ=255)

                                                      gCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCAt                                 <  1:5268941/93‑1 (MQ=255)

                                                      gCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCAt                                 >  1:4763852/1‑93 (MQ=255)

                                                      gCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCAt                                 <  2:4186909/93‑1 (MQ=255)

                                                      gCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCAt                                 >  1:878541/1‑93 (MQ=255)

                                                       cAGCCGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGCGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATg                                >  1:28739/1‑93 (MQ=255)

                                                       cAGACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATg                                <  2:4968077/93‑1 (MQ=255)

                                                         gACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGAt                              <  2:2560042/93‑1 (MQ=255)

                                                         gACGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGAt                              <  1:3914092/93‑1 (MQ=255)

                                                          aCGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGAtt                             >  1:1066557/1‑93 (MQ=255)

                                                          aCGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGAtt                             <  1:3300218/93‑1 (MQ=255)

                                                           cGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGAttt                            >  1:2901854/1‑93 (MQ=255)

                                                           cGCTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGAttt                            <  1:5731025/93‑1 (MQ=255)

                                                             cTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCTCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaa                          <  1:3378427/93‑1 (MQ=255)

                                                             cTCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaa                          <  1:5432911/93‑1 (MQ=255)

                                                              tCGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaat                         <  1:936409/93‑1 (MQ=255)

                                                               cGGTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTtaata                        <  2:6064726/93‑1 (MQ=255)

                                                                 gTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATaaa                      <  1:5809632/93‑1 (MQ=255)

                                                                 gTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATaaa                      >  1:5715391/1‑93 (MQ=255)

                                                                 gTAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATaaa                      <  1:6009335/93‑1 (MQ=255)

                                                                  tAGCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAAt                     <  2:3948614/93‑1 (MQ=255)

                                                                    gCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGa                   >  2:6244488/1‑93 (MQ=255)

                                                                    gCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGa                   >  1:3160661/1‑93 (MQ=255)

                                                                    gCGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGa                   <  2:1849416/93‑1 (MQ=255)

                                                                     cGACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAg                  <  1:1023764/93‑1 (MQ=255)

                                                                      gACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGt                 >  2:5714640/1‑93 (MQ=255)

                                                                      gACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGt                 <  1:4084679/93‑1 (MQ=255)

                                                                      gACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGt                 >  2:597591/1‑93 (MQ=255)

                                                                      gACATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGt                 >  2:5317631/1‑93 (MQ=255)

                                                                        cATGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTgg               >  2:4537405/1‑93 (MQ=255)

                                                                         aTGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGc              >  2:5272643/1‑93 (MQ=255)

                                                                          tGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCa             >  2:2413481/1‑93 (MQ=255)

                                                                          tGCGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCa             >  2:821838/1‑93 (MQ=255)

                                                                            cGTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCaaa           <  1:5144334/93‑1 (MQ=255)

                                                                             gTATAAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGGGGCaaaa          >  2:2736396/1‑93 (MQ=255)

                                                                               ataAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGc        >  2:489371/1‑93 (MQ=255)

                                                                               ataAATTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATAATTTAATAAATGAGTGGCAAAAGc        >  2:3965210/1‑93 (MQ=255)

                                                                                 aaaTTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGt      <  2:1240907/93‑1 (MQ=255)

                                                                                   aTTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa    <  1:3839918/93‑1 (MQ=255)

                                                                                   aTTTGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGa    <  1:5921831/93‑1 (MQ=255)

                                                                                    tttGCGCGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGAc   <  1:1277464/93‑1 (MQ=255)

TTCCCGTTCTTTTCAGACATCACGCCCGCGGGTGATGCTGTTGATGAAGTTGCCGCAGACGCTCGGTAGCGACATGCGTATAAATTTGCGTGGTGGAGAGATCGCTGTGGCCCAGTAGCATCTGCACCACGCGTAAATCCGCACCATGATTTAATAAATGAGTGGCAAAAGCGTGACG  >  NC_000913/3036848‑3037025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`