BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,046,045	T→C	V339V (GTA→GTG)	gcvP ←	glycine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,046,045	0	T	C	99.5%	580.9 / NA	182	V339V (GTA→GTG)	gcvP	glycine decarboxylase
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (98/83); total (99/83)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

ATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGC  >  NC_000913/3045955‑3046133

atCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCAc                                                                                        <  2:3299655/93‑1 (MQ=255)

atCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCAc                                                                                        >  2:5905236/1‑93 (MQ=255)

 tCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCAcc                                                                                       <  1:5685236/93‑1 (MQ=255)

   ggTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTg                                                                                     <  1:4526707/93‑1 (MQ=255)

   ggTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTg                                                                                     <  1:4858450/93‑1 (MQ=255)

    gTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTgg                                                                                    >  2:2827962/1‑93 (MQ=255)

    gTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTgg                                                                                    >  1:4214699/1‑93 (MQ=255)

    gTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTgg                                                                                    >  1:3449818/1‑93 (MQ=255)

    gTCAGACGGGGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTgg                                                                                    >  1:6272220/1‑93 (MQ=255)

     tCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTggg                                                                                   <  1:5961964/93‑1 (MQ=255)

      cAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGa                                                                                  <  1:1257641/93‑1 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                 <  1:643553/93‑1 (MQ=255)

       aGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGaa                                                                                 <  2:3089729/93‑1 (MQ=255)

        gACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAg                                                                                >  1:5112229/1‑93 (MQ=255)

        gACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAg                                                                                >  1:1352182/1‑93 (MQ=255)

         aCGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGt                                                                               <  1:2956105/93‑1 (MQ=255)

          cGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTa                                                                              >  1:750666/1‑93 (MQ=255)

           ggTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTAc                                                                             >  2:35119/1‑93 (MQ=255)

           ggTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCGGGGAAGTAc                                                                             >  1:3042740/1‑93 (MQ=255)

              gAATGCGGTTAGCGATACGTTTCAGGCCAGCCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACaaa                                                                          <  1:840404/93‑1 (MQ=255)

              gAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACaaa                                                                          <  2:1843968/93‑1 (MQ=255)

                 tGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGt                                                                       >  1:3255056/1‑93 (MQ=255)

                    ggTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTgg                                                                    >  2:191300/1‑93 (MQ=255)

                      ttAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAg                                                                  <  2:4550667/93‑1 (MQ=255)

                      ttAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAg                                                                  <  2:2385209/93‑1 (MQ=255)

                       tAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGt                                                                 >  2:4436894/1‑93 (MQ=255)

                        aGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGtt                                                                <  2:4763394/93‑1 (MQ=255)

                         gCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTc                                                               <  2:135851/93‑1 (MQ=255)

                          cGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCg                                                              <  2:427184/93‑1 (MQ=255)

                           gATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGc                                                             >  1:3708549/1‑93 (MQ=255)

                           gATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGc                                                             >  2:3565032/1‑93 (MQ=255)

                           gATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGc                                                             >  1:4807237/1‑93 (MQ=255)

                           gATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGc                                                             >  2:1756866/1‑93 (MQ=255)

                            aTACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCt                                                            >  2:2026177/1‑93 (MQ=255)

                             tACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCtt                                                           <  2:3646097/93‑1 (MQ=255)

                             tACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCtt                                                           <  1:1534440/93‑1 (MQ=255)

                              aCGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCttt                                                          <  1:2619140/93‑1 (MQ=255)

                              aCGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCttt                                                          <  1:2208358/93‑1 (MQ=255)

                              aCGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCttt                                                          <  1:5601564/93‑1 (MQ=255)

                               cGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTtc                                                         <  1:2826928/93‑1 (MQ=255)

                                 tttCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTtctc                                                       <  1:4423588/93‑1 (MQ=255)

                                   tCAGGCCAACCGGGCCGTGATAAACGGCATACAGGGTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAc                                                     <  1:6063755/93‑1 (MQ=255)

                                   tCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAc                                                     <  1:2191711/93‑1 (MQ=255)

                                   tCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAc                                                     >  2:4371334/1‑93 (MQ=255)

                                   tCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAc                                                     <  1:5828270/93‑1 (MQ=255)

                                   tCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAc                                                     <  2:5123166/93‑1 (MQ=255)

                                    cAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcg                                                    <  2:2642389/93‑1 (MQ=255)

                                       gCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcggcg                                                 >  2:4365464/1‑93 (MQ=255)

                                       gCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcggcg                                                 >  1:4491151/1‑93 (MQ=255)

                                       gCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcggcg                                                 >  2:786619/1‑93 (MQ=255)

                                        ccAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCAcggcgg                                                <  2:1664103/93‑1 (MQ=255)

                                         cAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACggagga                                               >  1:411022/1‑93 (MQ=255)

                                           aCCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGata                                             <  2:2632332/93‑1 (MQ=255)

                                            ccGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGatat                                            >  2:983712/1‑93 (MQ=255)

                                             cGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATg                                           >  2:6132811/1‑93 (MQ=255)

                                             cGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATg                                           >  2:5021696/1‑93 (MQ=255)

                                              gggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGGCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGt                                          >  2:6054947/1‑93 (MQ=255)

                                              gggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGt                                          <  1:3206275/93‑1 (MQ=255)

                                              gggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGt                                          >  1:1098171/1‑93 (MQ=255)

                                               gggcGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGtt                                         <  1:1818035/90‑1 (MQ=255)

                                               ggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTTCAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGtt                                         <  1:51668/93‑1 (MQ=255)

                                               ggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGtt                                         >  1:6256061/1‑93 (MQ=255)

                                               ggCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGtt                                         <  2:1694463/93‑1 (MQ=255)

                                                gCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTg                                        >  2:4008725/1‑93 (MQ=255)

                                                gCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTg                                        >  2:1223805/1‑93 (MQ=255)

                                                 ccGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGc                                       <  2:3682382/93‑1 (MQ=255)

                                                   gTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTc                                     <  1:2430827/93‑1 (MQ=255)

                                                    tGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTcg                                    >  2:4122890/1‑93 (MQ=255)

                                                    tGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTcg                                    <  2:6214514/93‑1 (MQ=255)

                                                     gATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTcgc                                   >  1:1889264/1‑93 (MQ=255)

                                                     gATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTcgc                                   >  2:3483222/1‑93 (MQ=255)

                                                       tAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGa                                 <  1:3862147/93‑1 (MQ=255)

                                                         aaCGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGt                               >  1:201594/1‑93 (MQ=255)

                                                          aCGACATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTc                              <  1:3919454/93‑1 (MQ=255)

                                                           cGGCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCt                             <  1:3294296/93‑1 (MQ=255)

                                                            ggCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTg                            >  2:536372/1‑93 (MQ=255)

                                                            ggCATACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTg                            <  2:5185183/93‑1 (MQ=255)

                                                                tACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATc                        >  1:3233791/1‑93 (MQ=255)

                                                                tACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATc                        >  1:3549479/1‑93 (MQ=255)

                                                                tACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATc                        <  1:5513204/93‑1 (MQ=255)

                                                                tACAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATc                        <  1:5718882/93‑1 (MQ=255)

                                                                 aCAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCg                       >  1:870737/1‑93 (MQ=255)

                                                                  cAGGCTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGc                      <  2:4341498/93‑1 (MQ=255)

                                                                      cTGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATg                  <  2:3018793/93‑1 (MQ=255)

                                                                       tGGCTATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGAAGTTCGCTTTCTCACGGCTAATATGTTGCTCGCGAGTCTGCATCTCCATgc                 <  2:3871990/93‑1 (MQ=255)

                                                                       tGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGTGAGTCTGCATCGCCATgc                 >  2:5477147/1‑93 (MQ=255)

                                                                       tGGCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATgc                 <  2:3710289/93‑1 (MQ=255)

                                                                        ggCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATgcg                >  1:4381664/1‑93 (MQ=255)

                                                                        ggCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATgcg                >  1:1108613/1‑93 (MQ=255)

                                                                         gCGATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATgcgc               >  1:5337178/1‑93 (MQ=255)

                                                                           gATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAg             <  2:3812113/93‑1 (MQ=255)

                                                                           gATGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAg             >  1:1986220/1‑93 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc            <  2:2256260/93‑1 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc            >  2:206285/1‑93 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgc            >  2:1514258/1‑93 (MQ=255)

                                                                            aTGTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGACATGCTGCTCGCGGGGCTGCATCGCCATGCGCAgg            >  2:716767/1‑92 (MQ=255)

                                                                              gTTTGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAgcgc          <  1:4319392/93‑1 (MQ=255)

                                                                                 tGCCAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGt       <  2:2452351/93‑1 (MQ=255)

                                                                                   ccAGCAGCACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTAt     >  2:63445/1‑93 (MQ=255)

                                                                                      gcagcaCCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGc  <  1:4091210/93‑1 (MQ=255)

ATCGGTCAGACGGTGAATGCGGTTAGCGATACGTTTCAGGCCAACCGGGCCGTGATAAACGGCATACAGGCTGGCGATGTTTGCCAGCAGTACCTGGGAAGTACAAATGTTGGAGTTCGCTTTCTCACGGCGGATATGTTGCTCGCGAGTCTGCATCGCCATGCGCAGCGCGGTATTGC  >  NC_000913/3045955‑3046133

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`