BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,069,904	T→C	T247A (ACC→GCC)	pgk ←	phosphoglycerate kinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,069,904	0	T	C	100.0%	828.7 / NA	255	T247A (ACC→GCC)	pgk	phosphoglycerate kinase
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (115/140); total (115/140)

Only 100 of 272 total aligned reads displayed.

CATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGA  >  NC_000913/3069814‑3069993

caTCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCca                                                                                         >  2:2194558/1‑93 (MQ=255)

caTCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCca                                                                                         <  1:5906728/93‑1 (MQ=255)

caTCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCca                                                                                         >  1:2636032/1‑93 (MQ=255)

 aTCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCcag                                                                                        >  1:3046923/1‑93 (MQ=255)

  tCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCcagc                                                                                       <  1:1474869/93‑1 (MQ=255)

   cGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCcagca                                                                                      <  2:1409362/93‑1 (MQ=255)

    gTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCcagcag                                                                                     >  2:4529215/1‑93 (MQ=255)

     ttAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGa                                                                                    <  2:2909950/93‑1 (MQ=255)

     ttAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGa                                                                                    >  1:2171768/1‑93 (MQ=255)

      tAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGAc                                                                                   <  2:5738940/93‑1 (MQ=255)

       aaCAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACg                                                                                  <  2:683199/93‑1 (MQ=255)

       aaCAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACg                                                                                  >  1:2730701/1‑93 (MQ=255)

        aCAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGt                                                                                 <  1:5422289/93‑1 (MQ=255)

         cAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGtt                                                                                <  2:853222/93‑1 (MQ=255)

         cAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGtt                                                                                <  1:5755136/93‑1 (MQ=255)

          aGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGttt                                                                               <  1:3716272/93‑1 (MQ=255)

           gATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTa                                                                              <  2:4608342/93‑1 (MQ=255)

           gATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTa                                                                              <  2:1897024/93‑1 (MQ=255)

           gATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTa                                                                              >  1:5988846/1‑93 (MQ=255)

            aTTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAg                                                                             >  1:188873/1‑93 (MQ=255)

            aTTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAg                                                                             >  2:3316706/1‑93 (MQ=255)

              ttCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCt                                                                           <  1:3463187/93‑1 (MQ=255)

                     tAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCac                                                                    >  1:5095698/1‑92 (MQ=255)

                       gCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAAcc                                                                  <  1:3189870/93‑1 (MQ=255)

                        ccGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCa                                                                 <  2:5119803/93‑1 (MQ=255)

                        ccGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCa                                                                 <  2:4763845/93‑1 (MQ=255)

                           gTGCAGTTTCAGAGAACTCGGTTGCTACGCGTACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGt                                                              <  1:1679467/93‑1 (MQ=255)

                           gTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGt                                                              >  2:6104419/1‑93 (MQ=255)

                           gTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGt                                                              <  1:1003790/93‑1 (MQ=255)

                           gTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGt                                                              <  1:2060368/93‑1 (MQ=255)

                            tGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTc                                                             >  1:2000140/1‑93 (MQ=255)

                              cAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAg                                                           >  2:4718438/1‑93 (MQ=255)

                               aGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGc                                                          >  1:476602/1‑93 (MQ=255)

                               aGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGc                                                          >  2:4429124/1‑93 (MQ=255)

                                gTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACTGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCt                                                         <  1:399310/93‑1 (MQ=255)

                                 tttCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCtt                                                        <  2:5348105/93‑1 (MQ=255)

                                 tttCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCtt                                                        <  1:5014715/93‑1 (MQ=255)

                                 tttCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCtt                                                        >  2:4339224/1‑93 (MQ=255)

                                   tCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCg                                                      <  2:5541626/93‑1 (MQ=255)

                                    cAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGt                                                     >  2:2480700/1‑93 (MQ=255)

                                     agagAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTa                                                    >  1:4695544/1‑93 (MQ=255)

                                     agagAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTa                                                    <  2:4072902/93‑1 (MQ=255)

                                      gagaACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTAc                                                   >  2:4224823/1‑93 (MQ=255)

                                      gagaACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTAc                                                   >  1:1559852/1‑93 (MQ=255)

                                       agaACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACa                                                  <  2:1685557/93‑1 (MQ=255)

                                        gaACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAg                                                 <  2:5599139/93‑1 (MQ=255)

                                          aCTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAggg                                               >  1:5239417/1‑93 (MQ=255)

                                           cTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGa                                              <  1:1589095/93‑1 (MQ=255)

                                           cTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGa                                              <  1:4551103/93‑1 (MQ=255)

                                           cTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGa                                              >  1:2966118/1‑93 (MQ=255)

                                            tCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGAt                                             >  2:485589/1‑93 (MQ=255)

                                             cGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGAtt                                            <  2:3456639/93‑1 (MQ=255)

                                              ggTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCGGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGAttt                                           >  2:5898032/1‑93 (MQ=255)

                                               gTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTa                                          >  1:4821435/1‑93 (MQ=255)

                                                ttGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTAc                                         <  2:5205777/93‑1 (MQ=255)

                                                 tGCTACGCGAACATCAGACGGAACCGGTATGTAGCAGGTGGCCAGCAGACGTTTAGCTTCGTCATCCAGGTCAGCTTCGTACAGGGATTTccc                                        <  1:4604842/93‑1 (MQ=255)

                                                  gCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTAccc                                       >  2:1468465/1‑93 (MQ=255)

                                                    tACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCcac                                     >  2:3814128/1‑93 (MQ=255)

                                                    tACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCcac                                     >  2:4922968/1‑93 (MQ=255)

                                                    tACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCcac                                     >  2:5817631/1‑93 (MQ=255)

                                                    tACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCcac                                     <  1:762894/93‑1 (MQ=255)

                                                     aCGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCcaca                                    >  1:4277589/1‑93 (MQ=255)

                                                       gcgAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATc                                  <  1:126316/93‑1 (MQ=255)

                                                       gcgAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATc                                  >  1:627680/1‑93 (MQ=255)

                                                         gAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGt                                >  1:3960197/1‑93 (MQ=255)

                                                         gAACATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGt                                >  1:2705103/1‑93 (MQ=255)

                                                           aCATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTgg                              >  2:2035612/1‑93 (MQ=255)

                                                           aCATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTgg                              >  2:5407492/1‑93 (MQ=255)

                                                            cATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGc                             >  1:6167312/1‑93 (MQ=255)

                                                            cATCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGc                             <  2:2762804/93‑1 (MQ=255)

                                                              tCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCt                           <  1:672424/93‑1 (MQ=255)

                                                              tCAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCt                           <  2:5184253/93‑1 (MQ=255)

                                                               cAGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCtt                          >  1:2343818/1‑93 (MQ=255)

                                                                aGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTtg                         <  2:130297/93‑1 (MQ=255)

                                                                aGACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTtg                         <  2:5302024/93‑1 (MQ=255)

                                                                 gACGGAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTtgt                        <  2:3110023/93‑1 (MQ=255)

                                                                    ggAACCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGcc                     <  1:4192483/93‑1 (MQ=255)

                                                                      aaCCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCcgc                   <  2:1285447/93‑1 (MQ=255)

                                                                      aaCCGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCcgc                   <  1:5689871/93‑1 (MQ=255)

                                                                         cGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGAt                >  1:2803616/1‑93 (MQ=255)

                                                                         cGGGATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGAt                <  2:429963/93‑1 (MQ=255)

                                                                          gggATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATa               >  2:711774/1‑93 (MQ=255)

                                                                          gggATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATa               <  1:5364286/93‑1 (MQ=255)

                                                                          gggATGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATa               >  2:2111354/1‑93 (MQ=255)

                                                                             aTGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAg            <  2:3151887/93‑1 (MQ=255)

                                                                              tGTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAgg           <  1:4950380/93‑1 (MQ=255)

                                                                               gTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCATCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGgt          <  1:3614715/93‑1 (MQ=255)

                                                                               gTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGgt          <  1:5119306/93‑1 (MQ=255)

                                                                               gTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGgt          <  2:2940601/93‑1 (MQ=255)

                                                                               gTTGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGgt          <  2:1777905/93‑1 (MQ=255)

                                                                                ttGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGgtg         <  1:3308801/93‑1 (MQ=255)

                                                                                ttGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGgtg         <  2:3678169/93‑1 (MQ=255)

                                                                                ttGCAGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGgtg         >  2:1579975/1‑93 (MQ=255)

                                                                                    aGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAg     <  2:905830/93‑1 (MQ=255)

                                                                                    aGGTGGCCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAg     >  1:990542/1‑93 (MQ=255)

                                                                                     ggtggcCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATACAGGTGTTAGc    <  1:376386/93‑1 (MQ=255)

                                                                                     ggtggcCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGc    <  2:3204911/93‑1 (MQ=255)

                                                                                     ggtggcCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGc    <  1:2508716/93‑1 (MQ=255)

                                                                                     ggtggcCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGc    <  1:1063960/93‑1 (MQ=255)

                                                                                       tggcCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGa  <  2:2552434/93‑1 (MQ=255)

CATCGTTAACAGATTTCAGGGTAGCCGGTGCAGTTTCAGAGAACTCGGTTGCTACGCGAACATCAGACGGAACCGGGATGTTGCAGGTGGTCAGCAGACGTTTAGCTTCGTCAACCAGGTCAGCTTCGTACAGGGATTTACCCACATCGTGGCCTTGTGCCGCGATAAAGGTGTTAGCGA  >  NC_000913/3069814‑3069993

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`