BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,082,961	A→G	S324P (TCC→CCC)	speA ←	biosynthetic arginine decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,082,961	0	A	G	100.0%	797.2 / NA	250	S324P (TCC→CCC)	speA	biosynthetic arginine decarboxylase
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (150/100); total (150/100)

Only 100 of 264 total aligned reads displayed.

GCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACAT  >  NC_000913/3082871‑3083050

gCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCt                                                                                         <  1:2734457/93‑1 (MQ=255)

  ggCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGc                                                                                       <  1:4207832/93‑1 (MQ=255)

  ggCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGc                                                                                       <  1:2092352/93‑1 (MQ=255)

   gCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCg                                                                                      <  1:5106133/93‑1 (MQ=255)

     aGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGaa                                                                                    >  2:4464854/1‑93 (MQ=255)

     aGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGaa                                                                                    >  1:5486257/1‑93 (MQ=255)

       aCCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACg                                                                                  <  2:3952216/93‑1 (MQ=255)

        ccGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGa                                                                                 >  1:4105644/1‑93 (MQ=255)

        ccGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGa                                                                                 <  2:5474845/93‑1 (MQ=255)

         cGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTGATTGAGGCCGTAGGTCACCGAACAGTCGGGCTGCGAACGAg                                                                                >  1:137815/1‑93 (MQ=255)

         cGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAg                                                                                <  2:2142540/93‑1 (MQ=255)

         cGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAg                                                                                <  1:1629267/93‑1 (MQ=255)

          gTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGt                                                                               <  1:4937504/93‑1 (MQ=255)

           ttttCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTa                                                                              >  1:4286156/1‑93 (MQ=255)

                ttcACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTc                                                                         <  1:4565020/93‑1 (MQ=255)

                 tcACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCa                                                                        <  2:1207940/93‑1 (MQ=255)

                   acacGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCGCCGAACAGTCGGGCTGCGAACGAGTACCTTCATa                                                                      <  2:1550402/93‑1 (MQ=255)

                   acacGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATa                                                                      >  2:5899508/1‑93 (MQ=255)

                    cacGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATaa                                                                     <  2:3802936/93‑1 (MQ=255)

                     acGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAAt                                                                    >  1:1915027/1‑93 (MQ=255)

                     acGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAAt                                                                    >  2:827228/1‑93 (MQ=255)

                       gCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATcc                                                                  <  2:2551963/93‑1 (MQ=255)

                        cATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCa                                                                 >  2:2023916/1‑93 (MQ=255)

                        cATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCa                                                                 >  2:1772384/1‑93 (MQ=255)

                        cATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCa                                                                 <  1:5034202/93‑1 (MQ=255)

                        cATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCa                                                                 <  2:362103/93‑1 (MQ=255)

                         aTCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCAc                                                                >  1:4029258/1‑93 (MQ=255)

                          tCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACg                                                               >  1:1971751/1‑93 (MQ=255)

                          tCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACg                                                               >  2:4045976/1‑93 (MQ=255)

                          tCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACg                                                               <  1:4819587/93‑1 (MQ=255)

                          tCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACg                                                               <  2:3786040/93‑1 (MQ=255)

                          tCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGCACCTTCATAATCCACg                                                               >  2:4097987/1‑93 (MQ=255)

                           cGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGc                                                              <  2:2128748/93‑1 (MQ=255)

                            gCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGcc                                                             >  1:6266678/1‑93 (MQ=255)

                            gCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGcc                                                             >  1:1586183/1‑93 (MQ=255)

                            gCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGcc                                                             >  1:2208653/1‑93 (MQ=255)

                             ccAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGccc                                                            >  2:3999991/1‑93 (MQ=255)

                              cAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCa                                                           >  1:4316979/1‑93 (MQ=255)

                              cAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCa                                                           <  2:342791/93‑1 (MQ=255)

                               aaTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAg                                                          >  2:1122090/1‑93 (MQ=255)

                               aaTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAg                                                          >  2:5182350/1‑93 (MQ=255)

                               aaTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAg                                                          <  1:3704548/93‑1 (MQ=255)

                               aaTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAg                                                          >  2:451286/1‑93 (MQ=255)

                                aTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGa                                                         >  1:447453/1‑93 (MQ=255)

                                aTCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGa                                                         <  2:2364810/93‑1 (MQ=255)

                                     ccAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgcc                                                    >  2:5582616/1‑93 (MQ=255)

                                     ccAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgcc                                                    >  2:1421361/1‑93 (MQ=255)

                                      cAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccg                                                   >  1:324499/1‑93 (MQ=255)

                                       aGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgc                                                  <  2:5734915/93‑1 (MQ=255)

                                       aGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgc                                                  >  2:266768/1‑93 (MQ=255)

                                       aGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgc                                                  <  1:1176610/93‑1 (MQ=255)

                                        gATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgcc                                                 >  2:5793831/1‑93 (MQ=255)

                                         aTAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgccg                                                >  2:1821112/1‑93 (MQ=255)

                                         aTAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgccg                                                <  2:353730/93‑1 (MQ=255)

                                          tAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGa                                               >  1:334366/1‑93 (MQ=255)

                                          tAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGa                                               <  2:3227728/93‑1 (MQ=255)

                                          tAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGa                                               <  1:1877000/93‑1 (MQ=255)

                                          tAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTAATAATCCACGCCCAGAccgccgccgc                                               >  1:3058256/1‑92 (MQ=255)

                                           caTGTTGTTGGGGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGAccgccgccggc                                              >  2:796062/2‑91 (MQ=255)

                                           aaTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGAc                                              <  1:5315501/93‑1 (MQ=255)

                                           aaTGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGAc                                              >  1:1064305/1‑93 (MQ=255)

                                              gttgttGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTc                                           <  1:5983433/93‑1 (MQ=255)

                                                tgttgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGa                                         <  2:4842601/93‑1 (MQ=255)

                                                tgttgGCGTATTCATGGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGa                                         <  1:3842140/93‑1 (MQ=255)

                                                  ttgGCGTATTCATTGAGGCCGTAGTTCCCCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAg                                       <  2:269540/93‑1 (MQ=255)

                                                   tgGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGc                                      >  2:958428/1‑93 (MQ=255)

                                                       gTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTg                                  >  1:1010382/1‑93 (MQ=255)

                                                        tATTCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGa                                 <  1:2420603/93‑1 (MQ=255)

                                                          ttCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAat                               >  1:2830760/1‑93 (MQ=255)

                                                           tCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAata                              <  2:2401050/93‑1 (MQ=255)

                                                           tCATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAata                              >  1:2055634/1‑93 (MQ=255)

                                                            cATTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAatat                             >  1:3871498/1‑93 (MQ=255)

                                                             aTTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATAtt                            >  2:3392899/1‑93 (MQ=255)

                                                             aTTGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATAtt                            <  1:2265044/93‑1 (MQ=255)

                                                             aTTGAGGCAGTAGCTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATAtt                            <  2:4382514/93‑1 (MQ=255)

                                                              ttGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTg                           >  1:3552963/1‑93 (MQ=255)

                                                               tGAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGa                          <  1:5511695/93‑1 (MQ=255)

                                                                gAGGCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGAc                         <  1:183429/93‑1 (MQ=255)

                                                                  ggCCGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGc                       >  1:746499/1‑93 (MQ=255)

                                                                     cGTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCa                    >  1:5743371/1‑93 (MQ=255)

                                                                      gTAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACAGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAg                   >  2:3049415/1‑93 (MQ=255)

                                                                       tAGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGc                  >  2:1667954/1‑93 (MQ=255)

                                                                        aGTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCt                 >  1:5893611/1‑93 (MQ=255)

                                                                         gTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCGCAGCtt                >  1:6046288/1‑93 (MQ=255)

                                                                         gTTCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCtt                <  1:3607586/93‑1 (MQ=255)

                                                                          ttCACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCtat               >  2:338121/1‑91 (MQ=255)

                                                                            cACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTtgtg             <  2:1315945/93‑1 (MQ=255)

                                                                            cACCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTtgtg             >  1:2729435/1‑93 (MQ=255)

                                                                             aCCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGc            >  2:4242615/1‑93 (MQ=255)

                                                                             aCCGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGc            <  2:3333810/93‑1 (MQ=255)

                                                                              ccGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCa           >  1:615127/1‑93 (MQ=255)

                                                                               cGAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAg          <  2:6263235/93‑1 (MQ=255)

                                                                                gAACAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGt         <  2:5123640/93‑1 (MQ=255)

                                                                                  aCAGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTc       <  1:4673610/93‑1 (MQ=255)

                                                                                    aGTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCca     <  2:3878389/93‑1 (MQ=255)

                                                                                     gTCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCgac    >  2:4196876/1‑93 (MQ=255)

                                                                                      tCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCcaca   <  1:4532337/93‑1 (MQ=255)

                                                                                      tCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCcaca   <  1:5622265/93‑1 (MQ=255)

                                                                                      tCGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCcaca   >  1:957833/1‑93 (MQ=255)

                                                                                       cGGGCTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACAt  >  1:1335841/1‑93 (MQ=255)

GCGGCAGACCGTTTTCTTCACACGCATCGCCAATCGCCCAGATAATGTTGTTGGCGTATTCATTGAGGCCGTAGTTCACCGAACAGTCGGACTGCGAACGAGTACCTTCATAATCCACGCCCAGACCGCCGCCGACGTCGAAGCACTGAATATTGACGCCCAGCTTGTGCAGTTCCACAT  >  NC_000913/3082871‑3083050

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`