BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,091,676	A→G	Q53R (CAG→CGG)	yqgF →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,091,676	0	A	G	100.0%	616.6 / NA	192	Q53R (CAG→CGG)	yqgF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (85/107); total (85/107)

Only 100 of 206 total aligned reads displayed.

TTACCGGCACCGCTCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCAGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCAGAGCGCGTAAATTTGCCAACCGTA  >  NC_000913/3091586‑3091765

ttACCGGCACCGCTCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTggcggc                                                                                          <  1:2948427/93‑1 (MQ=255)

 tACCGGCACCGCTCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGcc                                                                                         <  1:1749169/93‑1 (MQ=255)

    cGGCACCGCTCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGa                                                                                      <  1:3251095/93‑1 (MQ=255)

     ggCACCGCTCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGGGGCGGCCgggc                                                                                     >  2:2488802/1‑91 (MQ=255)

      gCACCGCTCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGGCGGACg                                                                                    >  2:2702951/1‑93 (MQ=255)

       cACCGCTCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGa                                                                                   <  1:2437198/93‑1 (MQ=255)

         ccGCTCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGACCATTATCGAGCGTTTACTGAAAGAGTGGCGGCAGGACGaaa                                                                                 <  2:6114628/93‑1 (MQ=255)

          cGCTCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGAAat                                                                                >  1:408849/1‑93 (MQ=255)

          cGCTCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAat                                                                                <  2:4483376/93‑1 (MQ=255)

           gCTCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGAGaaaaa                                                                               >  2:216579/1‑91 (MQ=255)

            cTCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGCAaaca                                                                              >  2:184013/1‑93 (MQ=255)

             tCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAatcat                                                                             <  2:1573618/93‑1 (MQ=255)

             tCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAaacat                                                                             >  2:5600633/1‑93 (MQ=255)

              tgCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAatcatc                                                                            <  1:4640440/92‑1 (MQ=255)

              cGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAatcatc                                                                            <  2:4554115/93‑1 (MQ=255)

               gCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGAAAACAtcg                                                                           >  1:2851774/1‑93 (MQ=255)

               gCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCAtcg                                                                           <  2:4121842/93‑1 (MQ=255)

                cccTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGGCGGAGGGAAAGAtcgt                                                                          >  2:1452229/1‑93 (MQ=255)

                cccTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGAAATCAtcgt                                                                          >  2:4702116/1‑93 (MQ=255)

                cccTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCAtcgt                                                                          <  1:2657009/93‑1 (MQ=255)

                cccTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCCGGCGAaataatgct                                                                          >  1:927664/1‑90 (MQ=255)

                 ccTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGCGTGGCGGCCGGCCGAACACCtcgtc                                                                         >  1:3001634/1‑93 (MQ=255)

                 ccTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCAtcgtc                                                                         <  2:3649364/93‑1 (MQ=255)

                  cTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGAAAACAtcttcg                                                                        >  1:38695/1‑93 (MQ=255)

                  cTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAataatcaccg                                                                        >  1:769136/1‑89 (MQ=255)

                  cTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCAacgtcg                                                                        >  2:720912/1‑93 (MQ=255)

                   tttGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGAAATCATCGTCgg                                                                       >  2:1364041/1‑93 (MQ=255)

                   tttGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCCGGGGAAAAAATCCTCgg                                                                       >  1:50258/1‑93 (MQ=255)

                     tGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGtt                                                                     <  2:3944949/93‑1 (MQ=255)

                       ccTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGAAAACATCGTCGGGTTg                                                                   >  1:221998/1‑93 (MQ=255)

                        cTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGc                                                                  <  2:2889377/93‑1 (MQ=255)

                         tGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCCGACGGAATAATCCTCGGGTTTcc                                                                 >  2:5026925/1‑93 (MQ=255)

                          gCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAAACATCGTCGGGTTGCCg                                                                >  1:5154193/1‑93 (MQ=255)

                          gCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCCGGGGCAATCAGCGCCGGGTTGcgg                                                                >  1:127228/1‑91 (MQ=255)

                            aaTTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGAAATCATCGTCGGGTTGCCggt                                                              >  2:2412943/1‑91 (MQ=255)

                            aaTTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCt                                                              <  2:3450451/93‑1 (MQ=255)

                             aTTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGAAATCATAGTCGGTTTGGCGCTg                                                             >  2:971177/1‑93 (MQ=255)

                              ttAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGAAATAATCGTCGGGTTGTCCCTGa                                                            >  2:2264630/1‑93 (MQ=255)

                               tAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGaa                                                           <  2:440057/93‑1 (MQ=255)

                                 aaGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAGAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAata                                                         <  2:2259790/93‑1 (MQ=255)

                                 aaGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGAAATCATCGTAGGGTTGCCGCTGAata                                                         >  2:5535531/1‑93 (MQ=255)

                                 aaGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAata                                                         <  1:4776772/93‑1 (MQ=255)

                                  aGCACAGTACGGTACGCCGGACTGCAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAatat                                                        <  1:2401091/93‑1 (MQ=255)

                                  aGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAatat                                                        <  1:2882910/93‑1 (MQ=255)

                                     ccaGGACGGTACGCCGGACTTGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGa                                                     <  2:3733562/92‑1 (MQ=255)

                                     acaGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGa                                                     <  2:1540038/93‑1 (MQ=255)

                                     acaGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGa                                                     <  2:6067092/93‑1 (MQ=255)

                                     acaGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGa                                                     <  2:5229838/93‑1 (MQ=255)

                                      caGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGAc                                                    <  1:3111319/93‑1 (MQ=255)

                                      caGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGAc                                                    <  2:1736627/93‑1 (MQ=255)

                                      caGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGAc                                                    >  1:4243845/1‑93 (MQ=255)

                                       aGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGGAATCATCGTCGGTTTGCCGCTGAATATGGGCg                                                   >  2:5284922/1‑93 (MQ=255)

                                       aGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACg                                                   <  1:5346623/93‑1 (MQ=255)

                                        ggACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTTGGGTTGCCGCTGGATATTGGCgg                                                  >  2:5740083/1‑93 (MQ=255)

                                         gACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGc                                                 <  2:277313/93‑1 (MQ=255)

                                         gACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGc                                                 >  2:4978882/1‑93 (MQ=255)

                                           cGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGGTTGCCGCTGAATATGGGCGGCAc                                               >  2:1566553/1‑93 (MQ=255)

                                            ggTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCAcc                                              <  2:782696/93‑1 (MQ=255)

                                             gTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGGCGGACGAAAACATAGGCGGTTTGGCGCTGGATATGGGGGGGACCg                                             >  1:1212837/1‑93 (MQ=255)

                                              tACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCAcccg                                            >  1:3138511/1‑91 (MQ=255)

                                              tACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGa                                            <  2:1632443/93‑1 (MQ=255)

                                              tACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGa                                            <  1:1507928/93‑1 (MQ=255)

                                               aCGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAg                                           <  2:4163062/93‑1 (MQ=255)

                                                cGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGc                                          <  1:1879358/93‑1 (MQ=255)

                                                  ccGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGAAATCATCGTCGGGTTGCCCCTGAATATGGACGGCACCCGGCaa                                        >  2:3833664/1‑93 (MQ=255)

                                                     gACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCa                                     >  1:802943/1‑93 (MQ=255)

                                                        tGGAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTg                                  <  2:4767994/93‑1 (MQ=255)

                                                         ggAACATTATCGAGCGTTTACTGAAAGAGTGGCGGGCGGACGAAATCATCGTCGGGTTGCCGCTGGATATGGGCGGGACCGAGGAACCATTGa                                 >  1:1228745/1‑93 (MQ=255)

                                                         ggAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGGAAACATCGTCGGGTTGCCGCTGGAATTTGAGGGCACCGCTGAACCATTGa                                 >  1:3786526/1‑93 (MQ=255)

                                                         ggAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGTCGCTGGATATGGACGGCCACCAGGCACCATTGa                                 >  1:5705583/1‑93 (MQ=255)

                                                         ggAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGa                                 <  2:3595906/93‑1 (MQ=255)

                                                          gAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGGATATTGAGGGCACCGAGCAACCATTGA‑c                               >  1:66076/1‑93 (MQ=255)

                                                          gAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑c                               <  1:1109238/93‑1 (MQ=255)

                                                          gAACATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAAACATCGTCGGGTTGCCGCTGCATATTGACGGCACCGAGCAAACATTGA‑c                               >  1:5846520/1‑93 (MQ=255)

                                                           aaCATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGGTTGCCGCTGAATATGGACGGCACCGAGCCACCATTGA‑Ct                              >  1:2324420/1‑93 (MQ=255)

                                                            aCATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTg                             <  2:3534884/93‑1 (MQ=255)

                                                            aCATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGGTTGCCGCTGAATATGGACGGGACCCAGCAACCCTTGA‑Ctc                             >  2:139411/1‑92 (MQ=255)

                                                             cATTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGGTTGCCGCTGAATATGGACGGCACCGAGGAAACCTTTA‑Cttc                            >  2:424442/1‑91 (MQ=255)

                                                             cATTATCGAGCGTTTACTGAAAGAGTGGCGGCCCGGCGAAATCATCGTCGGTTTGCCCCTGAATATGGACGGGACCGAGCAACCATTGA‑cgcc                            >  2:5860076/1‑90 (MQ=255)

                                                              aTTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGGCGAAATCATCGTCGGTTTTCCGCTGAATATTGACGGCACCCAGCAAACATTGA‑CTGcc                           >  2:1816162/1‑93 (MQ=255)

                                                              aTTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGGATATGGACGGCACCGAGCAACCATTTA‑CTTcc                           >  1:1012623/1‑93 (MQ=255)

                                                              aTTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGcc                           <  2:1210642/93‑1 (MQ=255)

                                                              aTTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGcc                           <  2:4717918/93‑1 (MQ=255)

                                                              aTTATCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAAACATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAAACACTGA‑CTGcc                           >  2:3297916/1‑93 (MQ=255)

                                                                 aTCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCaga                        <  1:2078228/93‑1 (MQ=255)

                                                                 aTCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCaga                        <  2:264111/93‑1 (MQ=255)

                                                                  tCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCagag                       <  2:832849/93‑1 (MQ=255)

                                                                  tCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCagag                       <  2:2443736/93‑1 (MQ=255)

                                                                  tCGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCagag                       <  1:5997724/93‑1 (MQ=255)

                                                                   cGAGCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCAGAgc                      <  1:4812669/93‑1 (MQ=255)

                                                                      gCGTTTACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCAGAGcgcg                   >  1:859153/1‑93 (MQ=255)

                                                                          ttACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCAGAGCGCGTaaa               <  1:3351275/93‑1 (MQ=255)

                                                                          ttACTGAAAGAGTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCAGAGCGCGTaaa               <  2:3169443/93‑1 (MQ=255)

                                                                             cTGAAAGAGTGGCGGCCGGACGAAATCATCGTCCGTTTGTCCCTGAATATGGACGGCGCCGGGCCACCAATGACCTGCCA‑AGCGCGGAAAtat            >  2:5110154/1‑91 (MQ=255)

                                                                               gAAAGAGTGGCGGCCGGACGAAATCAACGTCGGGTTGCCGCTGAATATGGGCGGGACCGAGCAACCCTTTA‑CTGCCAGAGGGCGTAAATTTGc          >  2:4521892/1‑93 (MQ=255)

                                                                                  agagTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCAGAGCGCGTAAATTTGCCaa       <  1:6257669/93‑1 (MQ=255)

                                                                                     gTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCAGAGCGCGTAAATTTGCCAACCg    >  1:6197385/1‑93 (MQ=255)

                                                                                     gTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCAGAGCGCGTAAATTTGCCAACCg    <  2:1127384/93‑1 (MQ=255)

                                                                                     gTGGCGGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCAGAGCGCGTAAATTTGCCAACCg    <  2:198969/93‑1 (MQ=255)

                                                                                       ggcggcCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCAGAGCGCGTAAATTTGCCAACCGTa  <  2:5391706/93‑1 (MQ=255)

TTACCGGCACCGCTCGCCCTTTGCCTGCAATTAAAGCACAGGACGGTACGCCGGACTGGAACATTATCGAGCGTTTACTGAAAGAGTGGCAGCCGGACGAAATCATCGTCGGTTTGCCGCTGAATATGGACGGCACCGAGCAACCATTGA‑CTGCCAGAGCGCGTAAATTTGCCAACCGTA  >  NC_000913/3091586‑3091765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`