BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,125,359	T→C	K226K (AAA→AAG)	glcD ←	glycolate oxidase subunit D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,125,359	0	T	C	100.0%	731.8 / NA	230	K226K (AAA→AAG)	glcD	glycolate oxidase subunit D
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (122/108); total (122/108)

Only 100 of 245 total aligned reads displayed.

GGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTC  >  NC_000913/3125269‑3125448

ggCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGctt                                                                                         <  1:3642138/93‑1 (MQ=255)

 gCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGcttt                                                                                        >  1:5067959/1‑93 (MQ=255)

 gCGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGcttt                                                                                        >  2:5548144/1‑93 (MQ=255)

  cGATGATGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTa                                                                                       <  2:4200899/93‑1 (MQ=255)

   gatgatGTCACCAACCGCAAGCCCGGCTTTTTTTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAc                                                                                      >  1:1452820/1‑93 (MQ=255)

   gatgatGTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAc                                                                                      <  1:530985/93‑1 (MQ=255)

    atgatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTAcc                                                                                     >  1:1207589/1‑93 (MQ=255)

      gatgTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGAGCCACGGGCGGCTTCGGCAGCAGCTTTACCGt                                                                                   >  2:4522893/1‑93 (MQ=255)

         gTCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCAc                                                                                <  2:6276056/93‑1 (MQ=255)

          tCACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACt                                                                               >  2:2858949/1‑93 (MQ=255)

           cACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACtt                                                                              >  1:4505313/1‑93 (MQ=255)

           cACCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACtt                                                                              <  1:3035768/93‑1 (MQ=255)

            accaccCGCAAGCCCGGCTGTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc                                                                             <  1:2973636/93‑1 (MQ=255)

            aCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc                                                                             <  2:5714471/93‑1 (MQ=255)

            aCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc                                                                             <  1:6203825/93‑1 (MQ=255)

            aCCAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTc                                                                             >  2:1869779/1‑93 (MQ=255)

             ccAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCg                                                                            >  1:2693342/1‑93 (MQ=255)

             ccAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCg                                                                            <  1:5876830/93‑1 (MQ=255)

              cAACCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGTGCGGCTTCGGCAGCAGCTTTACCGTCACTTCgg                                                                           >  2:4345257/1‑93 (MQ=255)

               aaCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggt                                                                          <  2:4426700/93‑1 (MQ=255)

               aaCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggt                                                                          >  2:1441342/1‑93 (MQ=255)

                aCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtg                                                                         <  2:1729974/93‑1 (MQ=255)

                aCCGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtg                                                                         <  2:5128991/93‑1 (MQ=255)

                 ccGCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCggtgg                                                                        <  1:2901058/93‑1 (MQ=255)

                   gCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCCCTTCGGTGGTc                                                                      >  2:246293/1‑93 (MQ=255)

                   gCAAGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTc                                                                      >  2:4658967/1‑93 (MQ=255)

                     aaGCCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCAc                                                                    >  2:4727644/1‑93 (MQ=255)

                      agcccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACg                                                                   >  2:1330969/4‑93 (MQ=255)

                      agcccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACg                                                                   >  2:1286938/4‑93 (MQ=255)

                       gcccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTGACGc                                                                  >  1:1394055/3‑93 (MQ=255)

                        cccGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGcc                                                                 >  1:4667730/2‑93 (MQ=255)

                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCgc                                                               >  2:3220621/1‑92 (MQ=255)

                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGa                                                               >  2:2237756/1‑93 (MQ=255)

                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGa                                                               >  1:5418523/1‑93 (MQ=255)

                          cGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACAGTCACTTCGGTGGGCACGCCGa                                                               >  1:315610/1‑93 (MQ=255)

                            gCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTGGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGc                                                             <  2:2107985/93‑1 (MQ=255)

                            gCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGc                                                             >  1:730426/1‑93 (MQ=255)

                            gCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGc                                                             >  1:4764891/1‑93 (MQ=255)

                                tttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATAc                                                         <  2:895620/93‑1 (MQ=255)

                                 ttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATAcc                                                        <  2:1583679/93‑1 (MQ=255)

                                 ttCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATAcc                                                        >  1:215983/1‑93 (MQ=255)

                                  tCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCt                                                       <  1:3265212/93‑1 (MQ=255)

                                   cTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCtt                                                      <  2:2123481/93‑1 (MQ=255)

                                   cTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCtt                                                      >  1:3776796/1‑93 (MQ=255)

                                    tACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTc                                                     <  2:5588176/93‑1 (MQ=255)

                                     aCCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTcc                                                    <  2:5073113/93‑1 (MQ=255)

                                       cGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGa                                                  <  2:6016217/93‑1 (MQ=255)

                                           tCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCg                                              >  1:2267936/1‑93 (MQ=255)

                                             aaaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGt                                            >  2:4761468/1‑93 (MQ=255)

                                             aaaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGt                                            >  2:1172266/1‑93 (MQ=255)

                                              aaGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTg                                           <  2:351291/93‑1 (MQ=255)

                                                gCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATCCCTTCCGATCCGGTGaa                                         >  1:776613/1‑93 (MQ=255)

                                                gCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGaa                                         <  2:1974727/93‑1 (MQ=255)

                                                 cTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAAc                                        <  1:156141/93‑1 (MQ=255)

                                                   ggCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAg                                      >  2:5782385/1‑93 (MQ=255)

                                                    gCTAACAGAGCCCGCGCCACGGGCGGCTTCCGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAgc                                     <  1:492360/93‑1 (MQ=255)

                                                     cTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAgcg                                    >  1:6025458/1‑93 (MQ=255)

                                                      tAACAGACCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAgcgc                                   <  1:557804/93‑1 (MQ=255)

                                                      tAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAgcgc                                   >  2:5513098/1‑93 (MQ=255)

                                                       aaCAGACCCCGCGACACGGGCGGCTTCGGCAGCAGCTTTACGGTCACTACGGTGTTCACGCGGAGCATACCTCCCGATCCGGTGAACAGCGcc                                  <  1:2420507/93‑1 (MQ=255)

                                                       aaCAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGcc                                  >  2:3715320/1‑93 (MQ=255)

                                                       aaCAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGcc                                  >  1:4798222/1‑93 (MQ=255)

                                                       aaCAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGcc                                  >  1:6129515/1‑93 (MQ=255)

                                                        aCAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCca                                 >  1:64228/1‑93 (MQ=255)

                                                         cAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcag                                >  1:1617308/1‑93 (MQ=255)

                                                          aGAACCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcagc                               >  1:2469874/1‑93 (MQ=255)

                                                           gatcccGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCCCGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcagca                              <  2:5420865/90‑1 (MQ=255)

                                                            aaCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcagcag                             >  2:1822036/1‑93 (MQ=255)

                                                            aaCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCcagcag                             <  1:889445/93‑1 (MQ=255)

                                                             aCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAgg                            <  2:853510/93‑1 (MQ=255)

                                                             aCCCGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGACGAGCATACCTTCCGATCCGGTGAACGGCGCCAGAAgg                            >  1:1267641/1‑93 (MQ=255)

                                                              cccGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGt                           >  2:5884064/1‑93 (MQ=255)

                                                              cccGCGCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGt                           <  2:2634083/93‑1 (MQ=255)

                                                                cgcgCCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCa                         >  1:52316/1‑93 (MQ=255)

                                                                 gcgcCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCaa                        >  1:3734009/1‑93 (MQ=255)

                                                                   gcCACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCaaaa                      >  2:5322900/1‑93 (MQ=255)

                                                                    ccACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAAc                     >  1:1102612/1‑93 (MQ=255)

                                                                    ccACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAAc                     <  2:3644848/93‑1 (MQ=255)

                                                                    ccACGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAAc                     <  1:3511061/93‑1 (MQ=255)

                                                                      aCGGGCGGCTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCa                   <  1:968499/93‑1 (MQ=255)

                                                                         ggcggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCGAAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAggg                >  1:5142240/1‑92 (MQ=255)

                                                                         ggcggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGt                <  1:2444958/93‑1 (MQ=255)

                                                                         ggcggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGt                <  2:2265445/93‑1 (MQ=255)

                                                                         ggcggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGt                >  1:187037/1‑93 (MQ=255)

                                                                          gcggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCCCGCCGAGCATCCCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTg               <  1:4080994/93‑1 (MQ=255)

                                                                           cggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGa              <  2:2741527/93‑1 (MQ=255)

                                                                            ggcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGaa             >  2:3133709/1‑93 (MQ=255)

                                                                             gcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGATCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAt            >  1:3375186/1‑93 (MQ=255)

                                                                             gcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAt            >  2:2793650/1‑93 (MQ=255)

                                                                             gcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAt            >  1:2220552/1‑93 (MQ=255)

                                                                             gcTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAAt            >  1:4315164/1‑93 (MQ=255)

                                                                              cTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATc           >  2:3091392/1‑93 (MQ=255)

                                                                              cTTCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATc           <  2:3499910/93‑1 (MQ=255)

                                                                               ttCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATcc          >  1:5700092/1‑93 (MQ=255)

                                                                                tCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCa         <  2:1012647/93‑1 (MQ=255)

                                                                                tCGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCa         <  1:3220621/93‑1 (MQ=255)

                                                                                 cGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCTATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAg        >  2:72398/1‑93 (MQ=255)

                                                                                 cGGCAGCAGCTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAg        >  1:3351085/1‑93 (MQ=255)

                                                                                     agcagcTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGcgcg    >  1:3550228/1‑93 (MQ=255)

                                                                                     agcagcTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGcgcg    >  2:238019/1‑93 (MQ=255)

GGCGATGATGTCACCAACCGCAAGTCCGGCTTTTTCTACCGAGTCAAAGCTGGCTAACAGAACCCGCGCCACGGGCGGCTTCGGCAGCAGTTTTACCGTCACTTCGGTGGTCACGCCGAGCATACCTTCCGATCCGGTGAACAGCGCCAGCAGGTCAAAACCAGGTGAATCCAGCGCGTC  >  NC_000913/3125269‑3125448

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`