BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,127,469	G→A	G254G (GGC→GGT)	b2981 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,127,469	0	G	A	98.7%	831.0 / NA	233	G254G (GGC→GGT)	b2981	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (121/109); minor base C (0/3); total (121/112)
Fisher's exact test for biased strand distribution p-value = 1.10e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 235 total aligned reads displayed.

GTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCAT  >  NC_000913/3127379‑3127558

gTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCAcc                                                                                         <  2:4043060/93‑1 (MQ=255)

  tCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCa                                                                                       >  2:1496498/1‑93 (MQ=255)

  tCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCa                                                                                       <  1:1378387/93‑1 (MQ=255)

   cGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCCCCCAt                                                                                      <  2:4840368/93‑1 (MQ=255)

   cGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCAt                                                                                      <  2:2549091/93‑1 (MQ=255)

    ggCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATg                                                                                     <  1:3562990/93‑1 (MQ=255)

    ggCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATg                                                                                     <  2:184503/93‑1 (MQ=255)

     gCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATgg                                                                                    <  1:4174149/93‑1 (MQ=255)

      cAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGt                                                                                   >  2:3473088/1‑93 (MQ=255)

        aGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCATATATTTAAGTTGATCACCCATGGTcg                                                                                 >  2:5093446/1‑93 (MQ=255)

         accGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTcgc                                                                                <  2:1996560/92‑1 (MQ=255)

            gACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGaa                                                                             >  1:5224326/1‑93 (MQ=255)

            gACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGaa                                                                             >  1:5653272/1‑93 (MQ=255)

            gACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGaa                                                                             >  2:531159/1‑93 (MQ=255)

            gACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGaa                                                                             >  1:3023983/1‑93 (MQ=255)

             aCAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAAt                                                                            >  1:4547847/1‑93 (MQ=255)

             aCAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAAt                                                                            <  1:326609/93‑1 (MQ=255)

             aCAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAAt                                                                            <  2:1221372/93‑1 (MQ=255)

              cAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAAtt                                                                           >  2:396324/1‑93 (MQ=255)

                aTAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCa                                                                         <  2:687175/93‑1 (MQ=255)

                aTAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCa                                                                         <  1:2218321/93‑1 (MQ=255)

                  aaaCGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATg                                                                       <  1:1106006/93‑1 (MQ=255)

                   aaCGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGt                                                                      >  2:2827514/1‑93 (MQ=255)

                    aCGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGtt                                                                     <  1:1331918/93‑1 (MQ=255)

                     cgcgCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTc                                                                    <  1:59659/93‑1 (MQ=255)

                      gcgcAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCg                                                                   <  1:853049/93‑1 (MQ=255)

                       cgcAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCCCCCATGGTCGCGAATTCATGTTCgg                                                                  <  2:1434306/93‑1 (MQ=255)

                       cgcAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCgg                                                                  >  2:4926231/1‑93 (MQ=255)

                       cgcAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCgg                                                                  <  1:6263767/93‑1 (MQ=255)

                       cgcAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCgg                                                                  >  1:5417018/1‑93 (MQ=255)

                        gcAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGt                                                                 >  1:2824017/1‑93 (MQ=255)

                        gcAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGt                                                                 >  2:2738819/1‑93 (MQ=255)

                        gcAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGt                                                                 <  1:2340178/93‑1 (MQ=255)

                        gcAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGt                                                                 <  2:5503992/93‑1 (MQ=255)

                        gcAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGt                                                                 >  1:3848740/1‑93 (MQ=255)

                          aGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTaa                                                               >  1:1636358/1‑93 (MQ=255)

                            ggTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAAc                                                             <  1:2587245/93‑1 (MQ=255)

                             gTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACg                                                            <  1:2918011/93‑1 (MQ=255)

                               gCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGc                                                          <  2:5837961/93‑1 (MQ=255)

                               gCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGc                                                          >  2:5577774/1‑93 (MQ=255)

                               gCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGc                                                          <  2:6177473/93‑1 (MQ=255)

                                 aGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGcac                                                        >  2:1766134/1‑93 (MQ=255)

                                 aGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGcac                                                        <  2:501054/93‑1 (MQ=255)

                                 aGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGcac                                                        >  2:4254392/1‑93 (MQ=255)

                                  gAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGcaca                                                       >  2:2776158/1‑93 (MQ=255)

                                  gAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGcaca                                                       >  2:3338550/1‑93 (MQ=255)

                                  gAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGcaca                                                       >  1:5011223/1‑93 (MQ=255)

                                   aatcaatcTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACaa                                                      >  1:4835720/1‑93 (MQ=255)

                                    atcaatcTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACaaa                                                     <  1:639620/93‑1 (MQ=255)

                                    atcaatcTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACaaa                                                     >  2:1396052/1‑93 (MQ=255)

                                     tcaatcTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAAt                                                    >  1:2646580/1‑93 (MQ=255)

                                       aatcTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATcc                                                  >  2:4220067/1‑93 (MQ=255)

                                        atcTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATccc                                                 >  2:4723881/1‑93 (MQ=255)

                                         tctcAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATcccc                                                >  2:2454750/1‑93 (MQ=255)

                                           tcAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAg                                              >  1:4461832/1‑93 (MQ=255)

                                            cAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGt                                             <  1:3953459/93‑1 (MQ=255)

                                                   ataGATCATATCGTGCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGtgctg                                      >  1:4399478/1‑93 (MQ=255)

                                                   ataGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGtgctg                                      >  1:1007238/1‑93 (MQ=255)

                                                   ataGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATACCCAGTTGtgctg                                      >  2:3810326/1‑93 (MQ=255)

                                                    taGATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGtgctgc                                     >  1:253193/1‑93 (MQ=255)

                                                      gATCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgcca                                   >  1:3114559/1‑93 (MQ=255)

                                                        tCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgccagc                                 >  1:1731881/1‑93 (MQ=255)

                                                        tCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgccagc                                 >  2:5130674/1‑93 (MQ=255)

                                                        tCATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgccagc                                 >  2:2573299/1‑93 (MQ=255)

                                                         cATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgccagcc                                >  1:918084/1‑93 (MQ=255)

                                                         cATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgccagcc                                <  1:3657906/93‑1 (MQ=255)

                                                         cATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgccagcc                                >  1:4716200/1‑93 (MQ=255)

                                                         cATATCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTgccagcc                                >  2:2154348/1‑93 (MQ=255)

                                                           tatCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGccagccag                              >  2:3549747/1‑93 (MQ=255)

                                                            atCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCaga                             >  2:4712451/1‑93 (MQ=255)

                                                            atCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCaga                             <  1:74283/93‑1 (MQ=255)

                                                            atCGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCaga                             >  2:3023054/1‑93 (MQ=255)

                                                              cGTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGt                           <  1:1147777/93‑1 (MQ=255)

                                                               gTCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGtt                          >  2:2257070/1‑93 (MQ=255)

                                                                tCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTg                         <  2:896669/93‑1 (MQ=255)

                                                                tCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTg                         >  2:1154625/1‑93 (MQ=255)

                                                                tCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTg                         <  1:5149159/93‑1 (MQ=255)

                                                                tCCGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTg                         <  2:1672510/93‑1 (MQ=255)

                                                                  cGGCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGaa                       <  2:2402322/93‑1 (MQ=255)

                                                                    gCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGaaaa                     >  2:5984147/1‑93 (MQ=255)

                                                                    gCACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGaaaa                     <  2:5951683/93‑1 (MQ=255)

                                                                     cACCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAAt                    <  2:2725105/93‑1 (MQ=255)

                                                                      aCCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAAtc                   >  2:3740842/1‑93 (MQ=255)

                                                                      aCCAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAAtc                   <  1:3827248/93‑1 (MQ=255)

                                                                       ccAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAAtct                  >  2:761522/1‑93 (MQ=255)

                                                                       ccAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAACCAGAGTTGAAAAtct                  >  2:4633261/1‑93 (MQ=255)

                                                                        cAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAAtctc                 >  2:4389370/1‑93 (MQ=255)

                                                                        cAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAAtctc                 <  2:2654663/93‑1 (MQ=255)

                                                                        cAGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAAtctc                 >  1:1539717/1‑93 (MQ=255)

                                                                         aGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCa                <  1:3519574/93‑1 (MQ=255)

                                                                         aGATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCa                <  1:4580463/93‑1 (MQ=255)

                                                                          gATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCAc               <  2:399850/93‑1 (MQ=255)

                                                                          gATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCAc               <  1:5269263/93‑1 (MQ=255)

                                                                          gATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCAc               <  1:1308613/93‑1 (MQ=255)

                                                                          gATATTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCAc               >  2:4446754/1‑93 (MQ=255)

                                                                             atTTAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCa            <  1:2011865/93‑1 (MQ=255)

                                                                               ttAAGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAgg          >  1:5830450/1‑93 (MQ=255)

                                                                                 aaGTTGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGAt        <  2:5637404/93‑1 (MQ=255)

                                                                                     tGATCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGc    <  2:796247/93‑1 (MQ=255)

                                                                                       aTCACCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCAt  >  1:4171078/1‑93 (MQ=255)

GTTCGGCAAGCCGACAATAAACGCGCAGGGTGCAGAATCAATCTCAGCGATATAGATCATATCGTCCGGCACCAGATATTTAAGTTGATCGCCCATGGTCGCGAATTCATGTTCGGTAAACGGCACAAATCCCCAGTTGTGCTGCCAGCCAGAGTTGAAAATCTCACGCAGGATCTGCAT  >  NC_000913/3127379‑3127558

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`