BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	361,946	A→G	L153P (CTG→CCG)	lacY ←	galactoside permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	361,946	0	A	G	100.0%	640.6 / NA	202	L153P (CTG→CCG)	lacY	galactoside permease
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (110/92); total (110/92)

Only 100 of 210 total aligned reads displayed.

AAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCT  >  NC_000913/361856‑362035

aaaaCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGgc                                                                                         <  2:2539872/93‑1 (MQ=255)

  aaCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGgcgc                                                                                       >  2:3762163/1‑93 (MQ=255)

   aCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGcc                                                                                      >  1:3994455/1‑93 (MQ=255)

   aCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGcc                                                                                      <  1:369022/93‑1 (MQ=255)

   aCGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGcc                                                                                      >  2:4537407/1‑93 (MQ=255)

     ggagAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCa                                                                                    <  1:5235852/90‑1 (MQ=255)

     ggCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCa                                                                                    >  2:2152980/1‑93 (MQ=255)

     ggCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCa                                                                                    <  1:232566/93‑1 (MQ=255)

      gCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAg                                                                                   >  1:1065195/1‑93 (MQ=255)

      gCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAg                                                                                   >  2:6029421/1‑93 (MQ=255)

      gCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAg                                                                                   >  2:4028605/1‑93 (MQ=255)

        gAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGcc                                                                                 >  1:3071034/1‑93 (MQ=255)

        gAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGcc                                                                                 >  2:1656749/1‑93 (MQ=255)

        gAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGcc                                                                                 >  1:1263/1‑93 (MQ=255)

          ggATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCAGACGGCGCCCAGCCaa                                                                               >  2:2947815/1‑93 (MQ=255)

          ggATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCaa                                                                               >  2:2852582/1‑93 (MQ=255)

           gATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAac                                                                              <  1:2712697/93‑1 (MQ=255)

            aTGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAaca                                                                             <  2:2587640/93‑1 (MQ=255)

             tGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAacac                                                                            >  2:3142207/1‑93 (MQ=255)

               aGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAg                                                                          <  2:4154629/93‑1 (MQ=255)

                gTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGc                                                                         <  1:347392/93‑1 (MQ=255)

                    acaGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCaaa                                                                     <  2:3270136/93‑1 (MQ=255)

                    acaGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCaaa                                                                     <  1:3560156/93‑1 (MQ=255)

                      aGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACa                                                                   <  2:5220861/93‑1 (MQ=255)

                       gCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACAt                                                                  >  1:6121649/1‑93 (MQ=255)

                        ccAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATc                                                                 <  1:2759028/93‑1 (MQ=255)

                        ccAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATc                                                                 <  1:677335/93‑1 (MQ=255)

                        ccAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATc                                                                 <  1:904325/93‑1 (MQ=255)

                        ccAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATc                                                                 <  2:3236924/93‑1 (MQ=255)

                          agagCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcg                                                               >  1:4852559/1‑93 (MQ=255)

                           gagCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgc                                                              <  1:6037086/93‑1 (MQ=255)

                            agCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcg                                                             >  1:5395750/1‑93 (MQ=255)

                            agCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcg                                                             >  1:483519/1‑93 (MQ=255)

                             gCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgc                                                            >  1:5834206/1‑93 (MQ=255)

                               ccagccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCcgcgcgc                                                          >  2:3314188/1‑93 (MQ=255)

                                 agccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGa                                                        >  1:2627894/1‑93 (MQ=255)

                                  gccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGAc                                                       >  1:3891117/1‑93 (MQ=255)

                                  gccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGAc                                                       >  1:3505383/1‑93 (MQ=255)

                                  gccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGAc                                                       <  2:1180225/93‑1 (MQ=255)

                                  gccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGAc                                                       <  2:5067773/93‑1 (MQ=255)

                                   ccagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGAcc                                                      <  2:5431300/93‑1 (MQ=255)

                                    cagAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCa                                                     >  1:4873698/1‑93 (MQ=255)

                                     agAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCaa                                                    >  1:3425763/1‑93 (MQ=255)

                                     agAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCaa                                                    >  2:6248430/1‑93 (MQ=255)

                                      gAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCaaa                                                   >  2:743102/1‑93 (MQ=255)

                                      gAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCaaa                                                   <  1:418960/93‑1 (MQ=255)

                                       aaaaCAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAAt                                                  <  1:5892452/93‑1 (MQ=255)

                                        aaacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAAtt                                                 >  1:5683180/1‑93 (MQ=255)

                                        aaacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAAtt                                                 >  1:2040275/1‑93 (MQ=255)

                                        aaacaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAAtt                                                 >  1:4927404/1‑93 (MQ=255)

                                          acaaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCg                                               >  1:3927390/1‑93 (MQ=255)

                                            aaacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGaa                                             >  2:3495108/1‑93 (MQ=255)

                                             accTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGaaa                                            <  1:2218597/91‑1 (MQ=255)

                                             aacTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGaaa                                            <  2:3483149/93‑1 (MQ=255)

                                               cTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAAtt                                          <  1:3255199/93‑1 (MQ=255)

                                                tGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTa                                         >  1:2883580/1‑93 (MQ=255)

                                                tGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTa                                         >  1:3236479/1‑93 (MQ=255)

                                                tGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTa                                         <  2:5949926/93‑1 (MQ=255)

                                                 gATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTAc                                        >  1:177138/1‑93 (MQ=255)

                                                 gATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTAc                                        >  2:3373513/1‑93 (MQ=255)

                                                   ttattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTg                                      <  2:1877810/93‑1 (MQ=255)

                                                   ttattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTg                                      >  2:5506693/1‑93 (MQ=255)

                                                    tattGATTGTGAACATGATGCCGACAATCGAGGCACACGGCGACCACCCAACACAGCCAAACATCCGCGCGCGACCAAATTCCAAATTAGTGc                                     <  2:1367150/93‑1 (MQ=255)

                                                    tattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGc                                     <  2:4292829/93‑1 (MQ=255)

                                                    tattGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGc                                     <  2:1025165/93‑1 (MQ=255)

                                                      ttGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCTACCAAATTCGAAAATACTGCGa                                   <  2:3109633/93‑1 (MQ=255)

                                                       tGATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGAc                                  <  1:1469046/93‑1 (MQ=255)

                                                        gATGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACg                                 <  1:4820951/93‑1 (MQ=255)

                                                         aTGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACgg                                >  2:4406675/1‑93 (MQ=255)

                                                          tGGTGAACATGATGCCGACAATCGAGGCTCACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGc                               <  2:5657725/93‑1 (MQ=255)

                                                          tGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGc                               <  2:4559319/93‑1 (MQ=255)

                                                          tGGTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGc                               <  2:3241254/93‑1 (MQ=255)

                                                            gTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTg                             >  2:4944592/1‑93 (MQ=255)

                                                            gTGAACATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTg                             >  1:519539/1‑93 (MQ=255)

                                                               aaCATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACt                          >  2:1139679/1‑93 (MQ=255)

                                                                aCATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCTGTCAAATTCAAACTTACCGCTACGGACGACtt                         >  1:4545247/1‑93 (MQ=255)

                                                                aCATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACtt                         <  1:2375459/93‑1 (MQ=255)

                                                                 cATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACttt                        >  1:2870164/1‑93 (MQ=255)

                                                                 cATGATGCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACttt                        >  2:3086368/1‑93 (MQ=255)

                                                                  atgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtc                       <  1:5943070/93‑1 (MQ=255)

                                                                   tgatgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTtct                      >  1:1923050/1‑93 (MQ=255)

                                                                     atgCCGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCa                    <  2:694174/93‑1 (MQ=255)

                                                                       gCCGACAATCGGGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAAt                  <  1:4626155/93‑1 (MQ=255)

                                                                        ccGACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATa                 <  1:71055/93‑1 (MQ=255)

                                                                          gACAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATaaa               <  1:787788/93‑1 (MQ=255)

                                                                           aCAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAAt              >  2:3644467/1‑93 (MQ=255)

                                                                           aCAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAAt              <  1:572432/93‑1 (MQ=255)

                                                                           aCAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAAt              >  2:2412508/1‑93 (MQ=255)

                                                                           aCAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAAt              <  2:2263909/93‑1 (MQ=255)

                                                                           aCAATCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAAt              >  1:5456254/1‑93 (MQ=255)

                                                                              aTCGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGcc           <  2:2127865/93‑1 (MQ=255)

                                                                                cGAGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCctc         <  2:6180893/93‑1 (MQ=255)

                                                                                  aGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTa       >  1:1641080/1‑93 (MQ=255)

                                                                                  aGGCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTa       >  1:1851434/1‑93 (MQ=255)

                                                                                   ggCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAc      >  1:5146820/1‑93 (MQ=255)

                                                                                    gCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAct     <  1:3739151/93‑1 (MQ=255)

                                                                                    gCACACGGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTAct     <  2:1808735/93‑1 (MQ=255)

                                                                                       cacgGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActgct  <  2:1996139/93‑1 (MQ=255)

                                                                                       cacgGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActgct  <  1:5408592/93‑1 (MQ=255)

                                                                                       cacgGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTActgct  <  1:1223502/93‑1 (MQ=255)

AAAACGGCGAGGATGAGTGCACAGCCAGAGCCCAGCCAGAAAACAAACTGATTATTGATGGTGAACATGATGCCGACAATCGAGGCACACAGCGCCCAGCCAACACAGCCAAACATCCGCGCGCGACCAAATTCGAAATTACTGCGACGGCTGACTTTCTCAATAAATGCCTCTACTGCT  >  NC_000913/361856‑362035

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`