Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,221,280 T→C L336P (CTG→CCG)  b3076 → evolved beta‑D‑galactosidase alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,221,2800TC99.6% 772.9 / ‑4.9 238L336P (CTG→CCG) b3076evolved beta‑D‑galactosidase alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (132/105);  minor base A (1/0);  total (133/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.64e-01

Only 100 of 247 total aligned reads displayed.

ACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGC  >  NC_000913/3221190‑3221369
                                                                                          |                                                                                         
aCGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGAtgccgc                                                                                         >  1:6109123/1‑93 (MQ=255)
  gTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCag                                                                                       >  1:1449840/1‑92 (MQ=255)
  gTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCAc                                                                                       >  2:85199/1‑93 (MQ=255)
     cTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGc                                                                                    >  1:6159828/1‑93 (MQ=255)
      tGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCg                                                                                   <  2:506630/93‑1 (MQ=255)
       ggAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGt                                                                                  <  1:5597023/93‑1 (MQ=255)
        gAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTc                                                                                 <  1:4150868/93‑1 (MQ=255)
          aGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCac                                                                               >  1:3045104/1‑92 (MQ=255)
          aGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCaa                                                                               <  2:128150/93‑1 (MQ=255)
             ggtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCg                                                                            >  2:4516114/1‑93 (MQ=255)
              gtgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGCCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGt                                                                           <  2:4878200/93‑1 (MQ=255)
               tgCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTc                                                                          <  2:5263141/93‑1 (MQ=255)
                gCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCa                                                                         <  1:1853599/93‑1 (MQ=255)
                gCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCa                                                                         <  2:1208831/93‑1 (MQ=255)
                 ccACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCAc                                                                        <  2:1108431/93‑1 (MQ=255)
                   acaACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGa                                                                      >  1:778382/1‑93 (MQ=255)
                     aaCGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACa                                                                    <  2:1011500/93‑1 (MQ=255)
                     aaCGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACa                                                                    >  2:1817980/1‑93 (MQ=255)
                     aaCGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACa                                                                    >  2:463132/1‑93 (MQ=255)
                          gTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGat                                                               >  2:2650653/1‑93 (MQ=255)
                           ttGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatc                                                              >  2:5502646/1‑93 (MQ=255)
                            tGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatca                                                             <  2:4271196/93‑1 (MQ=255)
                            tGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatca                                                             >  1:155363/1‑93 (MQ=255)
                            tGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatca                                                             >  1:4106590/1‑93 (MQ=255)
                            tGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatca                                                             <  1:6212272/93‑1 (MQ=255)
                              gCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatcatc                                                           <  2:4192311/93‑1 (MQ=255)
                              gCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatcatc                                                           >  2:5864566/1‑93 (MQ=255)
                              gCTTCCGTGATATCAAAGTGCGCGACGGTCTGCTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGatcatc                                                           >  1:5048423/1‑93 (MQ=255)
                                 tCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCa                                                        >  1:665906/1‑93 (MQ=255)
                                   cGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCaaa                                                      <  1:3182558/93‑1 (MQ=255)
                                   cGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCaaa                                                      <  1:4643262/93‑1 (MQ=255)
                                   cGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCaaa                                                      <  2:3778108/93‑1 (MQ=255)
                                   cGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCaaa                                                      <  1:5409418/93‑1 (MQ=255)
                                      gATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGc                                                   <  1:373785/93‑1 (MQ=255)
                                      gATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGc                                                   >  2:2484716/1‑93 (MQ=255)
                                         atCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCcgc                                                >  2:2061654/1‑93 (MQ=255)
                                           cAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCgcgc                                              <  1:4317126/93‑1 (MQ=255)
                                             aaGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCg                                            >  1:4755714/1‑93 (MQ=255)
                                               gTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGtt                                          <  2:2796215/93‑1 (MQ=255)
                                                   gcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                      <  2:5994065/93‑1 (MQ=255)
                                                   gcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                      <  2:2752825/93‑1 (MQ=255)
                                                   gcgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGaa                                      <  1:5746128/93‑1 (MQ=255)
                                                    cgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAAt                                     <  2:2947772/93‑1 (MQ=255)
                                                    cgACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAAt                                     >  2:3968972/1‑93 (MQ=255)
                                                     gACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATg                                    >  1:4239068/1‑93 (MQ=255)
                                                      aCGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATgg                                   <  1:3917398/93‑1 (MQ=255)
                                                      aCGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATgg                                   <  2:3935010/93‑1 (MQ=255)
                                                      aCGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATgg                                   <  2:2361885/93‑1 (MQ=255)
                                                      aCGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATgg                                   >  2:5763128/1‑93 (MQ=255)
                                                       cGGTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGa                                  <  2:6115427/93‑1 (MQ=255)
                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAt                                 <  1:206044/93‑1 (MQ=255)
                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAt                                 >  2:896717/1‑93 (MQ=255)
                                                        ggTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAt                                 <  1:193682/93‑1 (MQ=255)
                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGCTCATCGCAAAGGCCGCGCCGTTGGAATGGATc                                >  1:2808683/1‑93 (MQ=255)
                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCGAAGGCCGCGCCGTTGGAATGGATc                                >  1:856523/1‑93 (MQ=255)
                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGAtg                                >  1:3148685/1‑92 (MQ=255)
                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATc                                <  2:3748007/93‑1 (MQ=255)
                                                         gTCTGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATc                                <  1:1011398/93‑1 (MQ=255)
                                                            tGTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATcgcg                             >  1:2755312/1‑93 (MQ=255)
                                                             gTTCTGGCTCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATTGCAAAGGGCGCGCCGTTGGAATGGATcgcgc                            >  2:3556530/1‑92 (MQ=255)
                                                             gTTCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGt                            >  2:2670909/1‑93 (MQ=255)
                                                              ttCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGtg                           >  1:2447919/1‑92 (MQ=255)
                                                              ttCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTc                           <  2:3157499/93‑1 (MQ=255)
                                                               tCTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCgcggcg                          >  1:3380342/1‑93 (MQ=255)
                                                                cTGGATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCga                         <  2:4416667/93‑1 (MQ=255)
                                                                   gATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGaa                      <  2:1626987/93‑1 (MQ=255)
                                                                   gATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGaa                      >  1:1687824/1‑93 (MQ=255)
                                                                   gATCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGaa                      <  1:5275932/93‑1 (MQ=255)
                                                                    aTCAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGaaa                     >  2:3270683/1‑93 (MQ=255)
                                                                     tCAATAACCGTTTTGCGATGCCGCACGGCGTCAACCGTCACGACCACGATCATCGCAACGGCCGCGCCGTTGGAATGGATCGCGTCGAGaaaa                    >  2:5478248/1‑92 (MQ=255)
                                                                      cAATAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGa                   <  2:3631771/93‑1 (MQ=255)
                                                                       aaTAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAt                  >  1:4830800/1‑93 (MQ=255)
                                                                       aaTAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAt                  >  1:3607272/1‑93 (MQ=255)
                                                                        aTAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtc                 >  2:909959/1‑93 (MQ=255)
                                                                         tAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAGAGAttc                >  1:1290232/1‑91 (MQ=255)
                                                                         tAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtct                >  1:5455866/1‑93 (MQ=255)
                                                                         tAACCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtct                >  1:5293851/1‑93 (MQ=255)
                                                                          aaCCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtctc               <  2:4593136/93‑1 (MQ=255)
                                                                          aaCCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGAtctc               >  1:2533709/1‑93 (MQ=255)
                                                                           aCCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTcc              <  2:3919077/93‑1 (MQ=255)
                                                                           aCCGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTcc              <  2:846184/93‑1 (MQ=255)
                                                                            ccGTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCa             >  2:5659562/1‑93 (MQ=255)
                                                                             cGTTAGGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAg            <  2:71746/93‑1 (MQ=255)
                                                                              gTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCGGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGt           <  1:4553276/93‑1 (MQ=255)
                                                                              gTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGt           >  1:309593/1‑93 (MQ=255)
                                                                              gTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGt           <  1:1723678/93‑1 (MQ=255)
                                                                              gTTATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGt           >  1:4972544/1‑93 (MQ=255)
                                                                                tATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCCTCGCAAAGGCCGCGCCGTTGGAATGGATCGCCTCGGGCAAAACCTCCAGTtg         >  2:4710974/1‑93 (MQ=255)
                                                                                tATGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtg         >  2:6229358/1‑93 (MQ=255)
                                                                                 aTGTGATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtga        >  2:402833/1‑93 (MQ=255)
                                                                                  tgtgATGCCGCACGGCGTCAACCGTCACGACATCGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgat       <  1:1194564/93‑1 (MQ=255)
                                                                                  tgtgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgat       >  1:2315464/1‑93 (MQ=255)
                                                                                  tgtgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgat       >  2:3595964/1‑93 (MQ=255)
                                                                                  tgtgATGCAGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgat       >  2:2114841/1‑93 (MQ=255)
                                                                                    tgATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTtgatga     <  1:310918/93‑1 (MQ=255)
                                                                                     gATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGaa    >  2:638262/1‑93 (MQ=255)
                                                                                     gATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGaa    >  2:3092758/1‑93 (MQ=255)
                                                                                     gATGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGaa    <  2:4118485/93‑1 (MQ=255)
                                                                                      aTGCCGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAag   <  1:4792405/93‑1 (MQ=255)
                                                                                       tgccgcACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAagc  <  2:1189581/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACGTTCTGGAAGTGGTGCCACAACGCGTTGGCTTCCGTGATATCAAAGTGCGCGACGGTCTGTTCTGGATCAATAACCGTTATGTGATGCTGCACGGCGTCAACCGTCACGACAACGATCATCGCAAAGGCCGCGCCGTTGGAATGGATCGCGTCGAGAAAGATCTCCAGTTGATGAAGC  >  NC_000913/3221190‑3221369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑