BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,223,631	A→G	E90G (GAA→GGA)	ebgC →	evolved beta‑D‑galactosidase beta subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,223,631	0	A	G	100.0%	930.3 / NA	286	E90G (GAA→GGA)	ebgC	evolved beta‑D‑galactosidase beta subunit
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (128/158); total (128/158)

Only 100 of 288 total aligned reads displayed.

CCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATA  >  NC_000913/3223541‑3223720

ccGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGat                                                                                         <  2:1123225/93‑1 (MQ=255)

ccGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGat                                                                                         <  2:475455/93‑1 (MQ=255)

 cGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGata                                                                                        >  2:1957430/1‑93 (MQ=255)

  gggCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGatat                                                                                       >  1:2384021/1‑93 (MQ=255)

   ggCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGAtatt                                                                                      >  2:2399530/1‑93 (MQ=255)

     cATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGAtattat                                                                                    <  2:4420578/93‑1 (MQ=255)

     cATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGAtattat                                                                                    <  1:2843173/93‑1 (MQ=255)

       tCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCg                                                                                  <  1:4270895/93‑1 (MQ=255)

       tCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCg                                                                                  >  1:3165623/1‑93 (MQ=255)

        cGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGAGATTATCGt                                                                                 <  2:6107366/93‑1 (MQ=255)

         gACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTAACGtg                                                                                >  1:775008/1‑93 (MQ=255)

          aCGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtga                                                                               <  2:1342684/93‑1 (MQ=255)

           cGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgat                                                                              <  1:44398/93‑1 (MQ=255)

           cGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgat                                                                              >  1:4119030/1‑93 (MQ=255)

           cGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgat                                                                              >  1:4607757/1‑93 (MQ=255)

           cGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgat                                                                              <  1:213959/93‑1 (MQ=255)

           cGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgat                                                                              >  2:1029523/1‑93 (MQ=255)

            gCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgatg                                                                             <  1:4616440/93‑1 (MQ=255)

            gCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgatg                                                                             <  2:118972/93‑1 (MQ=255)

             cTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGtgatga                                                                            <  1:2422434/93‑1 (MQ=255)

              tATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGaa                                                                           <  1:5996090/93‑1 (MQ=255)

               aTTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTCATGaaa                                                                          <  2:5238620/93‑1 (MQ=255)

                ttttGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAAc                                                                         <  2:4628048/93‑1 (MQ=255)

                 tttGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACt                                                                        >  2:1925681/1‑93 (MQ=255)

                   tGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGAAGTGGGAACTTATCGTGATGACACTGa                                                                      >  2:1471839/1‑93 (MQ=255)

                    gAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGAc                                                                     <  1:1364570/93‑1 (MQ=255)

                    gAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGAc                                                                     >  2:1622548/1‑93 (MQ=255)

                     aaGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGAcc                                                                    >  1:3474171/1‑93 (MQ=255)

                        tGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTg                                                                 >  2:381648/1‑93 (MQ=255)

                          cATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGaa                                                               >  1:5140815/1‑93 (MQ=255)

                          cATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGaa                                                               >  2:4212210/1‑93 (MQ=255)

                          cATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGaa                                                               >  1:532068/1‑93 (MQ=255)

                           aTTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAat                                                              <  1:3716749/93‑1 (MQ=255)

                           aTTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAat                                                              <  1:1931289/93‑1 (MQ=255)

                           aTTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAat                                                              >  1:2790517/1‑93 (MQ=255)

                           aTTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGGAGTGGGATATTATCGTGATGAAACTGACCGTGAat                                                              >  2:1764358/1‑93 (MQ=255)

                            ttACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAata                                                             >  1:5790947/1‑93 (MQ=255)

                            ttACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAata                                                             <  2:4114084/93‑1 (MQ=255)

                            ttACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAata                                                             >  2:4275038/1‑93 (MQ=255)

                                 acCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaa                                                        >  2:2436868/1‑93 (MQ=255)

                                  ccTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaa                                                       >  1:2715696/1‑93 (MQ=255)

                                  ccTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaa                                                       <  1:5607423/93‑1 (MQ=255)

                                   cTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTaaaa                                                      >  1:2471572/1‑93 (MQ=255)

                                    tGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAg                                                     >  1:3437526/1‑93 (MQ=255)

                                    tGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAg                                                     >  2:2914624/1‑93 (MQ=255)

                                      cAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGc                                                   <  1:372980/93‑1 (MQ=255)

                                      cAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGc                                                   <  2:3157909/93‑1 (MQ=255)

                                      cAAGGGCAGCAAAAAATTGAATATGCACCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGc                                                   <  1:2003067/93‑1 (MQ=255)

                                       aaGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCt                                                  <  2:3549714/93‑1 (MQ=255)

                                        aGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTg                                                 <  2:4240958/93‑1 (MQ=255)

                                        aGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTg                                                 <  1:5168736/93‑1 (MQ=255)

                                        aGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTg                                                 >  1:3528219/1‑93 (MQ=255)

                                         gggCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGc                                                <  2:5883331/93‑1 (MQ=255)

                                            cagcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGga                                             <  2:5553220/93‑1 (MQ=255)

                                            cagcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGga                                             <  1:1598733/93‑1 (MQ=255)

                                            cagcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGga                                             <  2:571198/93‑1 (MQ=255)

                                            cagcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGga                                             <  2:2088749/93‑1 (MQ=255)

                                            cagcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGga                                             >  2:3631366/1‑93 (MQ=255)

                                             agcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGgag                                            >  2:513290/1‑93 (MQ=255)

                                             agcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGgag                                            >  1:5653719/1‑93 (MQ=255)

                                              gcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGgaga                                           >  2:895763/1‑93 (MQ=255)

                                              gcaAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGgaga                                           >  1:1297203/1‑93 (MQ=255)

                                               caAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGaa                                          <  1:3810328/93‑1 (MQ=255)

                                                aaaaaaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGaaa                                         >  1:1874697/1‑93 (MQ=255)

                                                 aaaaaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAAc                                        <  2:3342436/93‑1 (MQ=255)

                                                 aaaaaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAAc                                        >  1:2540695/1‑93 (MQ=255)

                                                   aaaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCg                                      <  1:2631235/93‑1 (MQ=255)

                                                    aaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGt                                     >  1:3958598/1‑93 (MQ=255)

                                                    aaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGt                                     >  2:4683196/1‑93 (MQ=255)

                                                    aaTTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGt                                     <  2:2766728/93‑1 (MQ=255)

                                                       tGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGa                                  <  1:3050308/93‑1 (MQ=255)

                                                       tGAATATGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGa                                  <  2:4041413/93‑1 (MQ=255)

                                                          atatGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGt                               >  1:1646861/1‑93 (MQ=255)

                                                           tatGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTc                              <  1:4775711/93‑1 (MQ=255)

                                                            atGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTcc                             >  1:2903501/1‑93 (MQ=255)

                                                             tGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCa                            <  1:5525187/93‑1 (MQ=255)

                                                             tGCGCCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCa                            <  1:2330496/93‑1 (MQ=255)

                                                              gcgcCGAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCAc                           <  1:5248674/93‑1 (MQ=255)

                                                                 cagAAAGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGaa                        <  2:5073815/91‑1 (MQ=255)

                                                                    aaaGAGACATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAggg                     <  2:6286993/93‑1 (MQ=255)

                                                                        agaCATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCaaa                 <  2:4328160/93‑1 (MQ=255)

                                                                         gaCATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAAt                <  2:485146/93‑1 (MQ=255)

                                                                          aCATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATc               <  2:2854473/93‑1 (MQ=255)

                                                                          aCATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATc               >  2:2546430/1‑93 (MQ=255)

                                                                          aCATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATc               <  1:2201142/93‑1 (MQ=255)

                                                                           cATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCg              >  1:1106783/1‑93 (MQ=255)

                                                                           cATTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCg              <  2:1520892/93‑1 (MQ=255)

                                                                            aTTACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGt             <  1:4524702/93‑1 (MQ=255)

                                                                             ttACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGtt            <  2:1677024/93‑1 (MQ=255)

                                                                              tACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTa           >  1:5981236/1‑93 (MQ=255)

                                                                              tACAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTa           <  2:825269/93‑1 (MQ=255)

                                                                               aCAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTAt          <  1:6112757/93‑1 (MQ=255)

                                                                                cAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTAtt         >  2:366366/1‑93 (MQ=255)

                                                                                cAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTAtt         >  1:5354139/1‑93 (MQ=255)

                                                                                cAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTAtt         <  2:2631296/93‑1 (MQ=255)

                                                                                cAGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTAtt         <  1:912936/93‑1 (MQ=255)

                                                                                 atgTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTAttt        <  1:4845774/91‑1 (MQ=255)

                                                                                 aGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTAttt        <  1:752609/93‑1 (MQ=255)

                                                                                 aGGTAGTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTAttt        <  2:2234921/93‑1 (MQ=255)

                                                                                      gTGGGATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGat   >  2:3611788/1‑93 (MQ=255)

CCGGGCATCGACGCTATTTTGAAGTGCATTACTACCTGCAAGGGCAGCAAAAAATTGAATATGCGCCGAAAGAGACATTACAGGTAGTGGAATATTATCGTGATGAAACTGACCGTGAATATTTAAAAGGCTGCGGAGAAACCGTTGAGGTCCACGAAGGGCAAATCGTTATTTGCGATA  >  NC_000913/3223541‑3223720

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`