BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,251,140	G→C	L239V (CTT→GTT)	yhaJ ←	putative transcriptional regulator LYSR‑type

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,251,140	0	G	C	100.0%	545.8 / NA	171	L239V (CTT→GTT)	yhaJ	putative transcriptional regulator LYSR‑type
Reads supporting (aligned to +/- strand): ref base G (0/0); new base C (130/41); total (130/41)

Only 100 of 180 total aligned reads displayed.

TCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGC  >  NC_000913/3251050‑3251229

tctcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAccc                                                                                         >  2:2052865/1‑93 (MQ=255)

tctcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAccc                                                                                         >  1:752737/1‑93 (MQ=255)

tctcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAccc                                                                                         >  1:1917291/1‑93 (MQ=255)

 ctcGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAcccc                                                                                        >  1:2318436/1‑93 (MQ=255)

    gCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGc                                                                                     >  1:947159/1‑93 (MQ=255)

     cTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGcc                                                                                    >  1:3409674/1‑93 (MQ=255)

     cTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGcc                                                                                    >  2:5609104/1‑93 (MQ=255)

      tGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCa                                                                                   >  1:1641488/1‑93 (MQ=255)

      tGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCa                                                                                   >  2:5699432/1‑93 (MQ=255)

        gTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGt                                                                                 >  1:3786117/1‑93 (MQ=255)

        gTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGt                                                                                 >  2:38886/1‑93 (MQ=255)

          cGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTaa                                                                               <  2:3163814/93‑1 (MQ=255)

          cGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTaa                                                                               <  2:1056969/93‑1 (MQ=255)

           gATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAAt                                                                              >  1:726514/1‑93 (MQ=255)

            aTTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAAtc                                                                             >  1:3872040/1‑92 (MQ=255)

            aTTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATg                                                                             >  1:4848497/1‑93 (MQ=255)

            aTTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATg                                                                             >  1:4106166/1‑93 (MQ=255)

              tCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGcc                                                                           >  1:3969006/1‑93 (MQ=255)

                 gggCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCTCCACGCCAACCCCCGCCCGTAATGCCTGa                                                                        >  1:4408691/1‑93 (MQ=255)

                 gggCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGa                                                                        >  1:1486035/1‑93 (MQ=255)

                    cTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGt                                                                     <  1:1915434/93‑1 (MQ=255)

                     tGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGtt                                                                    <  1:3152020/93‑1 (MQ=255)

                      gacgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGttt                                                                   >  2:4615504/1‑93 (MQ=255)

                        cgacACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTAt                                                                 >  1:2361232/1‑93 (MQ=255)

                           cacGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCtt                                                              >  2:3791306/1‑93 (MQ=255)

                            acGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTc                                                             >  2:5954327/1‑93 (MQ=255)

                              gCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCaa                                                           >  2:2676772/1‑93 (MQ=255)

                              gCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCaa                                                           >  1:2195119/1‑93 (MQ=255)

                               cAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAAt                                                          <  2:2487262/93‑1 (MQ=255)

                                aaCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATc                                                         <  1:5744232/93‑1 (MQ=255)

                                 aCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCg                                                        <  2:353967/93‑1 (MQ=255)

                                 aCCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCg                                                        >  1:3954567/1‑93 (MQ=255)

                                  ccGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGt                                                       <  1:2282707/93‑1 (MQ=255)

                                   cGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTg                                                      >  1:5200421/1‑93 (MQ=255)

                                   cGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTg                                                      >  1:1463729/1‑93 (MQ=255)

                                    gCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGc                                                     >  1:1004352/1‑93 (MQ=255)

                                    gCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGGAATGCCTGACGTTTATCTTCAATCGTGc                                                     >  1:324916/1‑93 (MQ=255)

                                    ccccTTCCGCAGTATCTGTTTCGACAGTAGGAGACGGCATCGGCGCGACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGc                                                     <  1:2843540/92‑1 (MQ=255)

                                     cccTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCt                                                    >  2:427138/1‑93 (MQ=255)

                                      ccTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTc                                                   >  1:3492463/1‑93 (MQ=255)

                                       cTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCa                                                  >  1:6218105/1‑93 (MQ=255)

                                        ttCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCAc                                                 >  1:529408/1‑93 (MQ=255)

                                         tCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCAcc                                                >  1:4106362/1‑93 (MQ=255)

                                            gCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGtt                                             >  2:4140269/1‑93 (MQ=255)

                                             cAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCATGCCAACCCCCGCCAGTAATGCCT‑ACGTTTATCTTCAATCGTGCTCACCGTTaa                                           >  2:6264871/1‑93 (MQ=255)

                                              aaTATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTaa                                           <  1:3141495/93‑1 (MQ=255)

                                              aaTATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTaa                                           >  1:1135083/1‑93 (MQ=255)

                                              aaTATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTaa                                           >  1:244645/1‑93 (MQ=255)

                                              aaTATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTaa                                           >  2:4749977/1‑93 (MQ=255)

                                               atatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAg                                          <  1:3190405/93‑1 (MQ=255)

                                               atatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAg                                          >  2:4169535/1‑93 (MQ=255)

                                               atatCTTTTACGACCATAGGATCCGGCCTCGCCGCCACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAg                                          <  2:4380138/93‑1 (MQ=255)

                                                tatCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAgc                                         >  1:1665044/1‑93 (MQ=255)

                                                   cTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGcgcg                                      >  1:1101822/1‑93 (MQ=255)

                                                    tttttCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCgg                                     >  1:1789428/1‑93 (MQ=255)

                                                    tttttCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCgg                                     >  2:1443089/1‑93 (MQ=255)

                                                     ttttCGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGc                                    <  1:910870/93‑1 (MQ=255)

                                                        tCGCCCATAGGATACGGCATCGTCCCCACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGt                                 <  2:3362357/93‑1 (MQ=255)

                                                         cGACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGtt                                >  2:135196/1‑93 (MQ=255)

                                                          gACCATAGGATACGGCATCGTCGCCACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGttt                               <  1:1190976/93‑1 (MQ=255)

                                                          gACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGGGCGGCTGttt                               >  2:1776309/1‑93 (MQ=255)

                                                          gACCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGttt                               <  2:4030291/93‑1 (MQ=255)

                                                           aCCATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTg                              >  1:5019481/1‑93 (MQ=255)

                                                            ccATAGGATACGGCATCGTCTCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGt                             <  2:666807/93‑1 (MQ=255)

                                                            ccATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGt                             >  2:5734957/1‑93 (MQ=255)

                                                            ccATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGt                             >  1:5097930/1‑93 (MQ=255)

                                                             cATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTc                            >  2:2380167/1‑93 (MQ=255)

                                                             cATAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTc                            >  2:5313890/1‑93 (MQ=255)

                                                              aTAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTcc                           >  2:3228315/1‑93 (MQ=255)

                                                              aTAGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGAGCGGCTGTTTGTcc                           >  2:5055786/1‑93 (MQ=255)

                                                               tAGGATACGGCATCGTCGCCACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCca                          <  2:5565156/93‑1 (MQ=255)

                                                                aGGATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcag                         >  1:3922390/1‑93 (MQ=255)

                                                                 ggATACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCtagc                        >  1:5527112/1‑93 (MQ=255)

                                                                  gATACGGCATCGTCGCCACGCCACCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCcagca                       <  1:3381090/93‑1 (MQ=255)

                                                                    tACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCagcagc                     >  1:3585133/1‑93 (MQ=255)

                                                                    tACGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCagcagc                     >  1:171912/1‑93 (MQ=255)

                                                                      cGGCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTg                   >  1:2925833/1‑93 (MQ=255)

                                                                       ggCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGt                  >  1:5514734/1‑93 (MQ=255)

                                                                        gCATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTa                 >  2:4892032/1‑93 (MQ=255)

                                                                         cATCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTAc                <  2:5948490/93‑1 (MQ=255)

                                                                          aTCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACg               >  2:891791/1‑93 (MQ=255)

                                                                          aTCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACg               <  1:4272481/93‑1 (MQ=255)

                                                                          aTCGTCGCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACg               >  2:2930820/1‑93 (MQ=255)

                                                                           tcgtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACgg              >  1:328041/1‑93 (MQ=255)

                                                                           tcgtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACgg              >  2:4705380/1‑93 (MQ=255)

                                                                           tcgtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACgg              >  1:20722/1‑93 (MQ=255)

                                                                           tcgtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACgg              >  2:1214679/1‑93 (MQ=255)

                                                                             gtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTc            >  2:4673937/1‑93 (MQ=255)

                                                                             gtcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTc            >  2:3078635/1‑93 (MQ=255)

                                                                              tcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCa           <  1:5158424/93‑1 (MQ=255)

                                                                              tcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCa           <  1:5772062/93‑1 (MQ=255)

                                                                              tcgCCACGCCAACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCa           <  1:5929081/93‑1 (MQ=255)

                                                                                gccacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAac         <  1:96621/93‑1 (MQ=255)

                                                                                gccacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAac         >  2:298651/1‑93 (MQ=255)

                                                                                gccacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAac         >  2:2357474/1‑93 (MQ=255)

                                                                                 ccacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAaca        >  1:4651199/1‑93 (MQ=255)

                                                                                  cacgccaACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAacac       >  1:625739/1‑93 (MQ=255)

                                                                                   ccgccaCCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACAcc      <  1:671428/92‑1 (MQ=255)

                                                                                    cgccaCCCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCg     <  2:3460118/93‑1 (MQ=255)

                                                                                       caACCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGgc  >  1:905723/1‑93 (MQ=255)

TCTCGCTGGTCGATTCCGGGCTGACGACACGCAACCGCCCTTCCGCAATATCTTTTTCGACCATAGGATACGGCATCGTCGCCACGCCAAGCCCCGCCAGTAATGCCTGACGTTTATCTTCAATCGTGCTCACCGTTAAGCGCGGCTGTTTGTCCAGCAGCTGTACGGTCAACACCGGGC  >  NC_000913/3251050‑3251229

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`