BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,421,120	Δ1 bp	noncoding (60/121 nt)	rrfF ←	5S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,421,119	0	G	.	91.8%	596.1 / 70.9	245	noncoding (61/121 nt)	rrfF	5S ribosomal RNA
Reads supporting (aligned to +/- strand): ref base G (13/7); new base . (110/114); total (123/122)
Fisher's exact test for biased strand distribution p-value = 2.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.93e-01

Only 100 of 277 total aligned reads displayed.

GCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCT  >  NC_000913/3421024‑3421210

gCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCTTGGGGAGACCCCACACTACCATCGGCGCTAcggcg                                                                                             >  2:567394/1‑96 (MQ=255)

 cccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgt                                                                                            <  2:9463023/96‑2 (MQ=39)

 cccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgt                                                                                            >  1:8316576/1‑95 (MQ=255)

 cccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgt                                                                                            <  2:8979985/96‑2 (MQ=39)

 cccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgt                                                                                            <  1:11913104/96‑2 (MQ=39)

 cccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCCCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgt                                                                                            <  1:3879777/96‑2 (MQ=255)

  ccAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTAcggcgtt                                                                                           >  1:14046289/1‑94 (MQ=255)

   cAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTAGTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                          >  2:13405661/1‑96 (MQ=255)

   cAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                          >  2:11708782/1‑96 (MQ=255)

   cAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                          <  1:11763439/96‑1 (MQ=37)

   cAGTCTTCCGACTGAGCCTTTTGCTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCTTGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                          <  2:10169829/96‑1 (MQ=255)

   cAGTCTTCCGACTGAGCCTTTTGCTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGttt                                                                                          <  1:10065470/96‑1 (MQ=255)

    aGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACATTACCATCGGCGCTACGGCGtttc                                                                                         >  1:10120902/1‑95 (MQ=255)

    aGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGtttc                                                                                         >  1:2654424/1‑95 (MQ=255)

    aGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGtttc                                                                                         <  2:5007352/96‑2 (MQ=25)

     gTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCa                                                                                       <  2:3474704/96‑1 (MQ=19)

     gTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCa                                                                                       <  2:2211521/96‑1 (MQ=19)

     gTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCa                                                                                       <  1:13477363/96‑1 (MQ=19)

      tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGCCCCCACACTACCATCGGCGCTACGGC‑GTTTCAc                                                                                      <  1:2124252/96‑1 (MQ=255)

      tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCAc                                                                                      >  1:11180416/1‑96 (MQ=255)

      tCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCACACACTACCATCGGCGCTACGGC‑GTTTCAc                                                                                      >  2:14103792/1‑96 (MQ=255)

       cTTCCGTCTGTGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTCCTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACt                                                                                     <  1:1844963/96‑1 (MQ=11)

       cTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACt                                                                                     <  2:11779543/96‑1 (MQ=19)

        ttCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACtt                                                                                    >  2:9123321/1‑96 (MQ=19)

          ccGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCt                                                                                  <  2:1287952/96‑1 (MQ=12)

          ccGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCt                                                                                  <  2:5399526/96‑1 (MQ=12)

           cGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTCCCATCGGCGCTACGGC‑GTTTCACTTCTg                                                                                 <  1:6898069/96‑1 (MQ=9)

           cGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTg                                                                                 >  1:9731642/1‑96 (MQ=12)

            gACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGa                                                                                >  1:1245060/1‑96 (MQ=9)

             aCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAg                                                                               >  1:935469/1‑96 (MQ=9)

             aCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAg                                                                               <  1:7724530/96‑1 (MQ=9)

             aCTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAg                                                                               >  1:5962376/1‑96 (MQ=12)

              cTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGt                                                                              <  2:10958788/96‑1 (MQ=9)

              cTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGt                                                                              <  2:10261497/96‑1 (MQ=9)

               tGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGtt                                                                             <  2:12432368/96‑1 (MQ=9)

               tGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGtt                                                                             >  2:8678414/1‑96 (MQ=12)

                gAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTc                                                                            >  2:3135239/1‑96 (MQ=12)

                 aGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCg                                                                           <  1:2419312/96‑1 (MQ=9)

                 aGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCg                                                                           <  1:4835900/96‑1 (MQ=9)

                 aGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCg                                                                           <  2:8464050/96‑1 (MQ=9)

                 aGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCg                                                                           <  2:8132018/96‑1 (MQ=9)

                 aGCCGTTTGTTTTATATGATGTCTGGCAGTTCCCTACTCTCACATGCGGAGCCCCCACACTACCCTCGGCGCTCCGGC‑GTTTCCCTTCTGAGTTCg                                                                           <  2:6552965/96‑1 (MQ=11)

                  gCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCgt                                                                          >  1:5443628/1‑95 (MQ=12)

                   ccTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGG‑TTCACTTCTGAGTTCGGc                                                                         >  2:12996687/1‑96 (MQ=19)

                   ccTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGc                                                                         <  2:4003362/96‑1 (MQ=9)

                    cTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCa                                                                        >  2:6966925/1‑96 (MQ=9)

                     ttttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCAt                                                                       >  2:11496507/1‑96 (MQ=9)

                     tcttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCAt                                                                       >  1:11852410/3‑96 (MQ=0)

                      tttGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATg                                                                      <  2:13979779/96‑1 (MQ=9)

                      tttGTGTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATg                                                                      <  1:2696545/96‑1 (MQ=9)

                                                                   gaCCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCttt                         >  1:5720732/1‑96 (MQ=0)

                                                                   gaCCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCttt                         >  2:1220652/1‑96 (MQ=0)

                                                                    aCCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCtttt                        <  2:5587894/96‑1 (MQ=9)

                                                                    aCCCCACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCtttt                        <  1:3187430/96‑1 (MQ=9)

                                                                     ccccACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTc                       <  2:7839103/96‑1 (MQ=12)

                                                                     ccccACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTc                       >  2:5613903/1‑96 (MQ=12)

                                                                      cccACACTACCATCGGCGCTAGGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                      <  2:2626373/96‑1 (MQ=255)

                                                                      cccACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                      <  1:52553/96‑1 (MQ=16)

                                                                      cccACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                      >  1:7342503/1‑96 (MQ=16)

                                                                      cccACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCt                      >  2:412098/1‑96 (MQ=16)

                                                                       ccACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTcca                     <  1:10700170/96‑3 (MQ=12)

                                                                       ccACACTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTa                     <  2:4074122/96‑1 (MQ=19)

                                                                        cccacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTaa                    <  2:5882911/94‑1 (MQ=255)

                                                                        cacaCTACCATCGGCGCTACGGC‑GTTTCACTTCTGTGTTCGGCATGGGGTCAGGAGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTaa                    >  1:3636279/1‑96 (MQ=11)

                                                                        cacaCTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTaa                    <  1:4148208/96‑1 (MQ=255)

                                                                        cacaCTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTaa                    <  1:1987750/96‑1 (MQ=255)

                                                                         acacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAAt                   <  2:9804899/96‑1 (MQ=255)

                                                                         acacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGGTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAAt                   >  2:5146225/1‑96 (MQ=255)

                                                                          cacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATc                  <  2:530778/96‑1 (MQ=255)

                                                                          cacTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATc                  <  1:5699672/96‑1 (MQ=255)

                                                                          cacTACCATCGGCGCTACGGC‑GTTTCAATTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATc                  <  1:14274516/96‑1 (MQ=255)

                                                                           acTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTCCTGCCGCCAGACAAATTCTTTTCTAATCt                 <  2:11960450/96‑1 (MQ=255)

                                                                           acTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCt                 >  1:7310708/1‑96 (MQ=255)

                                                                            cTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTg                <  1:8953723/96‑1 (MQ=255)

                                                                            cTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTg                <  1:2994922/96‑1 (MQ=255)

                                                                            cTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTg                >  1:13643852/1‑96 (MQ=255)

                                                                            cTACCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTg                >  1:1265778/1‑96 (MQ=255)

                                                                              aCCATCGGCGCTACGGC‑GTTTCGCTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGcc              <  1:11620429/96‑1 (MQ=255)

                                                                              aCCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGcc              >  1:14073936/1‑96 (MQ=255)

                                                                              aCCATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGcc              <  2:7681739/96‑1 (MQ=255)

                                                                               ccATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCCCCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCg             <  1:3677517/96‑1 (MQ=255)

                                                                               ccATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCg             <  1:9993891/96‑1 (MQ=255)

                                                                               ccATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCg             >  2:637559/1‑96 (MQ=255)

                                                                                cATCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGa            <  2:3813480/96‑1 (MQ=255)

                                                                                 aTCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGTCAAATTCTTTTCTAATCTGCCGaa           >  2:1538845/1‑96 (MQ=255)

                                                                                 aTCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGaa           >  1:6240478/1‑96 (MQ=255)

                                                                                 aTCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGaa           >  1:143380/1‑96 (MQ=255)

                                                                                  tCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAAc          <  2:206275/96‑1 (MQ=255)

                                                                                  tCGGCGCTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAAc          <  2:4540057/96‑1 (MQ=255)

                                                                                     gcgcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt       <  2:7265938/96‑1 (MQ=255)

                                                                                     gcgcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt       <  2:7398498/96‑1 (MQ=255)

                                                                                     gcgcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt       <  2:3087910/96‑1 (MQ=255)

                                                                                     gcgcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACttt       <  2:1369628/96‑1 (MQ=255)

                                                                                      cgcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTa      >  2:10663424/1‑96 (MQ=255)

                                                                                       gcTACGGC‑GTTTCACTTCTGAGTTCGGCCTGGGGTCAGGTGGGACCGCCGCGCTACTGCCGCCAGCCAAATTCTTTTCTAATCTGCCGAACTTTaa     <  1:6335067/96‑1 (MQ=17)

                                                                                       gcTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTaa     <  2:4322040/96‑1 (MQ=255)

                                                                                        cTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAAc    >  2:11181569/1‑96 (MQ=255)

                                                                                        cTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAAc    <  2:2001345/96‑1 (MQ=255)

                                                                                        cTACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAAc    >  1:4353455/1‑96 (MQ=255)

                                                                                         tACGGC‑GTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCTCGCTACTGACGCCAGACAAATTCTTTTCTCATCTGCCGAACTTGAAcc   <  1:12615421/96‑1 (MQ=17)

GCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCCTACTCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTACTGCCGCCAGACAAATTCTTTTCTAATCTGCCGAACTTTAACCT  >  NC_000913/3421024‑3421210

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`