BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,957,551	+T	intergenic (‑120/‑741)	ppiC ← / → rep	peptidyl‑prolyl cis‑trans isomerase C/rep helicase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,957,550	1	.	T	99.4%	1274.5 / ‑1.4	332	intergenic (‑119/‑742)	ppiC/rep	peptidyl‑prolyl cis‑trans isomerase C/rep helicase
Reads supporting (aligned to +/- strand): ref base . (2/0); new base T (110/218); total (112/220)
Fisher's exact test for biased strand distribution p-value = 1.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 364 total aligned reads displayed.

TTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CC‑‑T‑G‑‑C‑TCGCA‑G‑G‑T‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT  >  NC_000913/3957467‑3957632

ttttAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTTCATATATGGCAAAA‑GACCGGGAAAT‑CCG‑G‑G‑‑CGTCGCA‑G‑G‑T‑cc                                                                          >  2:4450395/1‑96 (MQ=255)

ttttAGCAGGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑T‑cc                                                                          <  2:6380841/96‑1 (MQ=255)

 tttAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTTGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑T‑ccc                                                                         <  2:12040783/96‑2 (MQ=255)

 tttAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAAGGCCCGGAAATC‑CC‑‑G‑G‑‑CTTCGCA‑G‑G‑T‑ccc                                                                         >  1:9508448/1‑95 (MQ=255)

 tttAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAA‑GCCCGGGAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑T‑ccc                                                                         >  2:6135437/1‑95 (MQ=255)

  ttAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑T‑ccct                                                                        >  2:7806618/1‑94 (MQ=255)

   tAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑T‑ccctc                                                                       <  2:906366/96‑4 (MQ=255)

   tAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑T‑ccctc                                                                       <  1:13511849/96‑4 (MQ=255)

   tAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAA‑GACCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑T‑ccctc                                                                       >  1:1007390/1‑93 (MQ=255)

    aGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑T‑ccctca                                                                      <  2:6929436/96‑5 (MQ=255)

      cATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAg                                                                     <  1:11921447/96‑1 (MQ=255)

      cATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAg                                                                     <  2:11681308/96‑1 (MQ=255)

       aTGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAgg                                                                    >  1:878115/1‑96 (MQ=255)

       aTGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CC‑‑GGG‑‑CTTCGCA‑G‑G‑TCCCTCAAgg                                                                    <  1:13772995/96‑1 (MQ=255)

        tGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAggg                                                                   >  2:7524271/1‑96 (MQ=255)

         gATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAGT‑CCG‑G‑G‑‑CTTCGCA‑G‑GAC‑CCTCAAgggg                                                                  <  2:12721233/96‑1 (MQ=255)

          aTCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAA‑ACCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCAGG‑G‑C‑CCTCAAGGGga                                                                 >  1:5436625/1‑96 (MQ=255)

           tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGgag                                                                <  2:5650829/96‑1 (MQ=255)

           tCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑ACCCGGAAAAT‑CCG‑G‑G‑‑CGTTGCA‑G‑G‑TCCCTCAAGGGgag                                                                >  1:2792066/1‑96 (MQ=255)

              gCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGaaa                                                             <  2:13032093/96‑1 (MQ=255)

              gCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGGAAAA‑GCCCGGGAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGgaa                                                             >  1:5365329/1‑96 (MQ=255)

              gCAGATGCTAACGAAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAATCCC‑‑G‑G‑‑CTTCGCA‑G‑G‑TCCCCCCAGGGGAGaaa                                                             >  2:8411741/1‑96 (MQ=255)

               cAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TGCCTCAAGGGGAGaaaa                                                            >  2:13495099/1‑96 (MQ=255)

                aGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAAc                                                           <  2:4055451/96‑1 (MQ=255)

                 taTGCTAACGTAAATGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCC‑G‑G‑TCCCTCAAGGGGGGAAAACt                                                          >  1:13887132/2‑96 (MQ=255)

                  aTGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACtg                                                         <  1:11290610/96‑2 (MQ=255)

                    gCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAACA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAg                                                       <  2:2039638/96‑1 (MQ=255)

                    gCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAg                                                       <  1:8552099/96‑1 (MQ=255)

                     cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAgg                                                      >  1:12488161/1‑96 (MQ=255)

                     cTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCACGGAAAAT‑CC‑‑G‑GGTT‑TCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAgg                                                      <  2:5289619/96‑1 (MQ=255)

                      tAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGGAAAA‑GCCCGGAAAAA‑CC‑‑TGG‑‑CGTCGCA‑GAC‑C‑CCCCAAGGGGAGAAAACTTCggg                                                     >  1:9712166/1‑96 (MQ=255)

                      tAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACGTAggg                                                     >  1:6613612/1‑96 (MQ=255)

                      tAACGTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAggg                                                     >  2:5991274/1‑96 (MQ=255)

                         catAATTGTCAGTCAGGGAGTGGCTGGTACATATCTGGGCAAA‑GCCCGGAAAAT‑CCGGG‑G‑‑C‑TCGCAGG‑G‑C‑CCTCAAGGGGAGAAAACCTAGGGCct                                                  >  1:3408379/3‑96 (MQ=255)

                         cGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCct                                                  >  1:12940359/1‑96 (MQ=255)

                          gTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGGAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCTAGGGGAGAAAAATTAAGGCctc                                                 >  2:4636861/1‑96 (MQ=255)

                          gTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCctc                                                 >  1:1093391/1‑96 (MQ=255)

                          gTAATTGTCAGTCAGGGAGTGGCTGGGACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCctc                                                 >  1:1814160/1‑96 (MQ=255)

                           tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCctct                                                >  2:11482654/1‑96 (MQ=255)

                           tAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCctct                                                <  2:14383100/96‑1 (MQ=255)

                            agtggtcagtcgGGGGGGGGCTGGTACATATATGCAAAAA‑GCCCGAAAAGG‑CCG‑G‑G‑‑CATCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTa                                               <  1:7223885/92‑1 (MQ=255)

                            aaTTGTCAGTCAGGGAGTGGCTGGTACCTATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCCAGGGGAGAAAAATTAGGGCCTCTa                                               >  1:11186625/1‑96 (MQ=255)

                            aaTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTa                                               <  1:9251436/96‑1 (MQ=255)

                            aaTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTa                                               <  2:10765216/96‑1 (MQ=255)

                             aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTAt                                              >  2:11573092/1‑96 (MQ=255)

                             aTTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTAt                                              >  2:2166011/1‑96 (MQ=255)

                              ttGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATg                                             <  1:5800985/96‑1 (MQ=255)

                              ttGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATg                                             <  2:10357353/96‑1 (MQ=255)

                               tGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATgg                                            <  1:13598148/96‑1 (MQ=255)

                                gtcagtcaGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATggg                                           >  2:7166103/1‑96 (MQ=255)

                                 tcagtcagGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                          >  2:9291577/1‑96 (MQ=255)

                                 tcagtcagGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGc                                          <  1:13224112/96‑1 (MQ=255)

                                  cagtcagGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCAGG‑G‑C‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCt                                         >  1:3811850/1‑96 (MQ=255)

                                    gtcagGGAGTGGCTGGTACATATATGGCAAAA‑ACCCGGAAAATCCC‑‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTAc                                       >  2:11830517/1‑96 (MQ=255)

                                     tcagGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                      <  1:7565511/96‑1 (MQ=255)

                                     tcagGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACa                                      <  2:6688139/96‑1 (MQ=255)

                                       agGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                                    <  2:1098528/96‑1 (MQ=255)

                                       agGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CC‑‑GGG‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACaaa                                    >  2:12429792/1‑96 (MQ=255)

                                          gAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTc                                 <  1:12232681/96‑1 (MQ=255)

                                           aGTGGCTGGTACATATATGTCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                                <  1:4496507/96‑1 (MQ=255)

                                           aGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCa                                <  1:14362567/96‑1 (MQ=255)

                                               gCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTAt                            <  1:8965929/96‑1 (MQ=255)

                                                cTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTagc                           <  1:11830517/96‑3 (MQ=255)

                                                cTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATc                           <  1:11552940/96‑1 (MQ=255)

                                                 tGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCg                          >  2:5893996/1‑96 (MQ=255)

                                                 tGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCg                          <  2:3343510/96‑1 (MQ=255)

                                                   gTACATATATGGCAAAA‑GCCCGGAAGAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                        <  1:6949602/96‑1 (MQ=255)

                                                   gTACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCggg                        <  1:7850976/96‑1 (MQ=255)

                                                    tACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCT‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTAAGGGCTACAAAGTCATTATCgggg                       >  2:12618459/1‑96 (MQ=255)

                                                    tACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                       >  2:6611685/1‑96 (MQ=255)

                                                    tACATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCgggg                       <  1:3325854/96‑1 (MQ=255)

                                                     aCATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGa                      >  2:5409527/1‑96 (MQ=255)

                                                      cATATATGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGAt                     <  1:4321708/96‑1 (MQ=255)

                                                       atatatGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAATCTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATg                    <  2:10817354/96‑1 (MQ=255)

                                                       atatatGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATg                    <  2:1404761/96‑1 (MQ=255)

                                                       atatatGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATg                    <  2:7470129/96‑1 (MQ=255)

                                                         atatGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt                  <  1:1671771/96‑1 (MQ=255)

                                                         atatGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGt                  >  2:9641887/1‑96 (MQ=255)

                                                           atGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtta                <  2:695480/96‑1 (MQ=255)

                                                           atGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtta                <  2:8213420/96‑1 (MQ=255)

                                                           atGGCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtta                >  1:2234212/1‑96 (MQ=255)

                                                           atGGCAAAA‑GCCCGGAAAAT‑CC‑‑GGG‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGtta                >  2:11265868/1‑96 (MQ=255)

                                                             ggCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt              <  2:3596404/96‑1 (MQ=255)

                                                             ggCAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatt              <  2:1944487/96‑1 (MQ=255)

                                                               cAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttt            <  2:6035300/96‑1 (MQ=255)

                                                               cAAAA‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttt            <  1:6141914/96‑1 (MQ=255)

                                                                aaaa‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGATAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGgtatttaa           >  1:10557638/1‑94 (MQ=255)

                                                                aaaa‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta           >  2:13610565/1‑96 (MQ=255)

                                                                aaaa‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta           <  2:2832256/96‑1 (MQ=255)

                                                                aaaa‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta           <  1:4978887/96‑1 (MQ=255)

                                                                aaaa‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta           <  1:3667821/96‑1 (MQ=255)

                                                                aaaa‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttatttta           >  2:4202633/1‑96 (MQ=255)

                                                                 aaa‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAATTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttat          <  2:1598951/96‑1 (MQ=255)

                                                                  aa‑GCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt         <  2:14152438/96‑1 (MQ=255)

                                                                  aa‑GCCCGGAAAAT‑CC‑‑GGG‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGttattttatt         >  1:3136911/1‑96 (MQ=255)

                                                                     gCCCGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGt       <  1:8273553/96‑1 (MQ=255)

                                                                      cccGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTc      <  2:11322051/96‑1 (MQ=255)

                                                                        cGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCaa    <  2:878115/96‑1 (MQ=255)

                                                                        cGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCa     >  2:2483130/1‑95 (MQ=255)

                                                                        cGGAAAAT‑CCG‑G‑G‑‑CTTCGCA‑G‑G‑TCCCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCa     <  1:2483130/95‑1 (MQ=255)

TTTTAGCATGATCCGCAGATGCTAACGTAATTGTCAGTCAGGGAGTGGCTGGTACATATATGGCAAAA‑GCCCGGAAAAT‑CC‑‑T‑G‑‑C‑TCGCA‑G‑G‑T‑CCTCAAGGGGAGAAAACTTAGGGCCTCTATGGGCTACAAAGTCATTATCGGGGATGGTTATTTTATTGTCAATT  >  NC_000913/3957467‑3957632

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`