BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,363	+C	coding (169/1314 nt)	gntT →	gluconate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,363	1	.	C	99.4%	1322.9 / ‑1.5	357	coding (169/1314 nt)	gntT	gluconate permease
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (175/179); total (177/180)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 375 total aligned reads displayed.

TCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑GC‑G‑GAC‑G‑‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGAT  >  NC_000913/3544280‑3544457

tCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGcc                                                                                       <  2:11008892/96‑1 (MQ=255)

tCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGcc                                                                                       >  1:6414264/1‑96 (MQ=255)

 ccTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCt                                                                                      <  1:3252996/96‑1 (MQ=255)

 ccTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCt                                                                                      <  2:4662697/96‑1 (MQ=255)

  cTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCtt                                                                                     <  1:5661220/96‑1 (MQ=255)

  cTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCtt                                                                                     >  1:710230/1‑96 (MQ=255)

  cTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCtt                                                                                     >  1:12461378/1‑96 (MQ=255)

    cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGc                                                                                   <  2:7985317/96‑1 (MQ=255)

    cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGc                                                                                   >  1:449597/1‑96 (MQ=255)

     gaggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGcc                                                                                  >  1:8829938/3‑96 (MQ=255)

     gTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGcc                                                                                  >  1:2738919/1‑96 (MQ=255)

      tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCTGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGccc                                                                                 <  1:12187471/96‑1 (MQ=255)

      tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGccc                                                                                 >  2:3558267/1‑96 (MQ=255)

       ggCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCt                                                                                >  1:5268949/1‑96 (MQ=255)

       ggCGCTTGCTCTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCt                                                                                <  2:10729356/96‑1 (MQ=255)

        gcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTg                                                                               <  2:3776203/96‑1 (MQ=255)

        gcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTg                                                                               >  2:1942500/1‑96 (MQ=255)

        gcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGGGGCGGCGG‑GAC‑GC‑TCGGTAGCCTTGccccg                                                                               >  2:9505353/1‑94 (MQ=255)

        gagcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTg                                                                               >  1:10573567/3‑96 (MQ=255)

         cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GGC‑GC‑TCGTTAGCCTTGCCCTGa                                                                              >  1:5969881/1‑96 (MQ=255)

          gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGAt                                                                             >  2:2553920/1‑96 (MQ=255)

          gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGAt                                                                             >  2:10005167/1‑96 (MQ=255)

          gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGAt                                                                             <  2:6871313/96‑1 (MQ=255)

           cTTGCTGTTGGATTAATGCAAGGCATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATc                                                                            <  2:13485075/96‑1 (MQ=255)

           cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATc                                                                            >  1:4061436/1‑96 (MQ=255)

            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCa                                                                           <  2:114683/96‑1 (MQ=255)

            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCa                                                                           <  2:7061962/96‑1 (MQ=255)

            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCa                                                                           >  1:11175121/1‑96 (MQ=255)

            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCCTGCCCTGATCa                                                                           >  1:4886104/1‑96 (MQ=255)

             tGCTGTTGGATTAATACAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCAt                                                                          >  1:8162212/1‑96 (MQ=255)

              gCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCAtt                                                                         >  1:10186256/1‑95 (MQ=255)

               cTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCGCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATgg                                                                        >  2:6587944/1‑96 (MQ=255)

                tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATggg                                                                       >  2:4565357/1‑96 (MQ=255)

                  ttGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGtt                                                                     <  1:6121168/96‑1 (MQ=255)

                   tGGATTAATGCAAGGAATGCCGCTGGATAGAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GGC‑GC‑TCCGTAGCCTTGCCCTGCTCATGGGttt                                                                    >  2:5252159/1‑96 (MQ=255)

                    ggATTAATGCAAGGACTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGtttt                                                                   <  1:3879167/96‑1 (MQ=255)

                    ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGtttt                                                                   <  1:9557917/96‑1 (MQ=255)

                    ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGtttt                                                                   <  2:13186885/96‑1 (MQ=255)

                    ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGtttt                                                                   <  2:9843810/96‑1 (MQ=255)

                     gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTg                                                                  <  1:11711303/96‑1 (MQ=255)

                     gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTg                                                                  >  1:8703525/1‑96 (MQ=255)

                      aTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCATGATCATGGGTTTTgg                                                                 >  1:12794623/1‑96 (MQ=255)

                         agtGCAAGGAATGCCGCTGGGTAAAGTTATTGGCTCCATCAAAACCGGGGTCGGC‑G‑GGG‑GGCTCCGTAGCCGTGCCCTGTTCCTGGGGTTTGgggc                                                              >  1:7515184/3‑96 (MQ=255)

                          aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                                             >  2:9367048/1‑96 (MQ=255)

                          aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCa                                                             >  2:14361416/1‑96 (MQ=255)

                           tccAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                                            >  1:11064607/3‑96 (MQ=255)

                           tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                                            >  1:14243133/1‑96 (MQ=255)

                           tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                                            <  2:235181/96‑1 (MQ=255)

                            gCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                                           >  1:151491/1‑96 (MQ=255)

                              aaGGACTGCCGCTTGATAAAGTTATCGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                                         <  1:1349967/96‑1 (MQ=255)

                              aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                                         >  2:7474504/1‑96 (MQ=255)

                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCCATGCt                                                        >  1:8478752/1‑96 (MQ=255)

                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCt                                                        <  1:12247778/96‑1 (MQ=255)

                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                                      <  1:13792329/96‑1 (MQ=255)

                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                                      >  1:9153000/1‑96 (MQ=255)

                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                                      <  2:13771322/96‑1 (MQ=255)

                                   aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                                    <  2:13939700/96‑1 (MQ=255)

                                    tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCa                                                   <  1:1716749/96‑1 (MQ=255)

                                      ccGCTGGATAAAGTTATTGGCTCCATCGAAGCCGGTGTCGGCGG‑GACGG‑‑CCGGGAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaa                                                 >  2:12711520/1‑96 (MQ=255)

                                      ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaa                                                 >  1:433977/1‑96 (MQ=255)

                                       cGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGGCTTGCCCTGGTCATGGGTTTTGGCGCAATGCTGGGCaaaa                                                >  2:5482624/1‑96 (MQ=255)

                                       cGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaaaa                                                <  2:11888663/96‑1 (MQ=255)

                                        gCTGGATAGAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGTGCAATGCTGGGCAAAAt                                               >  2:12961415/1‑96 (MQ=255)

                                        gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                                               <  1:7201753/96‑1 (MQ=255)

                                         cTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATg                                              <  2:400897/96‑1 (MQ=255)

                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGTCCTGATCTTGGGTTTTTGCGCAATGCTGGGCACAATGc                                             >  1:2647804/1‑96 (MQ=255)

                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                                             <  2:9223522/96‑1 (MQ=255)

                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                                             <  2:12427413/96‑1 (MQ=255)

                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                                             >  2:10460078/1‑96 (MQ=255)

                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                                             >  1:4563850/1‑96 (MQ=255)

                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                                             <  1:14086394/96‑1 (MQ=255)

                                          tGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                                             >  2:582205/1‑96 (MQ=255)

                                           ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                                            <  1:1942500/96‑1 (MQ=255)

                                            gATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGATTTGGCGCAATGCTGGGCAAAATGCTg                                           >  1:2253307/1‑96 (MQ=255)

                                             aTAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                                          >  1:7922512/1‑96 (MQ=255)

                                             aTAAAGTTATTGGCTCCATCAAAGCCGGTGTCGGC‑GAGAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGGGCAATGCTGGGCAAAATGCTgg                                          >  1:12941497/1‑96 (MQ=255)

                                             aTAAAGTTATTGGCGCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                                          <  1:2422263/96‑1 (MQ=255)

                                               aaaGTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGAGCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCa                                        >  2:12246082/1‑96 (MQ=255)

                                                  gTTATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAA‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                                     >  1:9879887/1‑96 (MQ=255)

                                                   ttATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                                    <  2:5660297/96‑1 (MQ=255)

                                                    tATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg                                   <  1:10050557/96‑1 (MQ=255)

                                                    tATTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg                                   >  1:3752637/1‑96 (MQ=255)

                                                     aTTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc                                  <  1:5044688/96‑1 (MQ=255)

                                                     aTTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc                                  <  2:3186326/96‑1 (MQ=255)

                                                     aTTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc                                  <  1:11966714/96‑1 (MQ=255)

                                                     aTTGGCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc                                  <  1:304147/96‑1 (MQ=255)

                                                         gCTCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg                              <  1:6684923/96‑1 (MQ=255)

                                                           tCCATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc                            >  2:811131/1‑96 (MQ=255)

                                                            cgaTCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg                           >  1:10718722/3‑96 (MQ=255)

                                                            ccATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGGTCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg                           >  2:2071964/1‑96 (MQ=255)

                                                             cATCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcgc                          >  2:8802691/1‑96 (MQ=255)

                                                              aTCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGca                         >  1:5446774/1‑96 (MQ=255)

                                                               tCAAAGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac                        <  2:6254962/96‑1 (MQ=255)

                                                                  aaGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAAc                     <  2:8574210/96‑1 (MQ=255)

                                                                  aaGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAAc                     <  1:13081371/96‑1 (MQ=255)

                                                                  aaGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAAc                     >  1:5322197/1‑96 (MQ=255)

                                                                   aGCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACg                    >  2:4934978/1‑96 (MQ=255)

                                                                    gCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGGGGCGCACAACGt                   >  2:10325846/1‑96 (MQ=255)

                                                                    gCCGGTGTCGGCGG‑GAC‑GC‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGGGGCGCACAACGt                   >  2:4198212/1‑96 (MQ=255)

                                                                                      gg‑‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGAt  >  1:12977787/2‑96 (MQ=255)

TCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑GC‑G‑GAC‑G‑‑TCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAACGTATCGCCACCACGCTGAT  >  NC_000913/3544280‑3544457

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`