BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,158,827	G→T	G61V (GGC→GTC)	yijC →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,158,827	0	G	T	99.8%	1849.8 / NA	496	G61V (GGC→GTC)	yijC	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (1/0); new base T (235/259); total (236/260)
Fisher's exact test for biased strand distribution p-value = 4.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 509 total aligned reads displayed.

CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAAC  >  NC_000913/4158734‑4158919

 ccgccgTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTAGCGCGTGAAGCGGTCAt                                                                                           <  2:1704431/96‑1 (MQ=255)

  cgccgTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCAtt                                                                                          >  1:13301168/1‑96 (MQ=255)

   gccgTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTg                                                                                         >  2:10715748/1‑96 (MQ=255)

   gccgTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCCGAACGCAGCTTCGCAAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTg                                                                                         <  2:6526938/96‑1 (MQ=255)

     cgTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAGGCGGTCATTGCt                                                                                       <  1:7324636/96‑1 (MQ=255)

     cgTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCt                                                                                       <  1:4938713/96‑1 (MQ=255)

      gTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGccc                                                                                      <  1:7544405/96‑3 (MQ=255)

      gTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTc                                                                                      >  2:12166884/1‑96 (MQ=255)

      gTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTc                                                                                      >  1:2321893/1‑96 (MQ=255)

        tCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTccc                                                                                    >  2:2003730/1‑96 (MQ=255)

         cGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCa                                                                                   >  2:3171460/1‑96 (MQ=255)

         cGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCa                                                                                   >  1:1300986/1‑96 (MQ=255)

         cGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCa                                                                                   <  1:1857937/96‑1 (MQ=255)

          gCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCAc                                                                                  >  1:8924538/1‑96 (MQ=255)

           cTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCAcc                                                                                 <  2:5138495/96‑1 (MQ=255)

            tggtggAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACct                                                                                >  2:7483250/1‑96 (MQ=255)

            tggtggAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACct                                                                                >  1:9770168/1‑96 (MQ=255)

              gtggAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACctct                                                                              <  1:5143346/96‑1 (MQ=255)

               tggAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCTTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCtt                                                                             <  2:10299361/96‑1 (MQ=255)

                ggAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCttt                                                                            >  2:11874310/1‑96 (MQ=255)

                 gAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCtttt                                                                           <  2:5692029/96‑1 (MQ=255)

                    gCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTAt                                                                        <  2:1417794/96‑1 (MQ=255)

                      cGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCg                                                                      >  1:11565796/1‑96 (MQ=255)

                      cGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCg                                                                      <  2:12463088/96‑1 (MQ=255)

                      cGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCg                                                                      >  2:6850998/1‑96 (MQ=255)

                      cGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCg                                                                      <  1:1672950/96‑1 (MQ=255)

                       tgaTTTAGCCAATTAAGTGCTGAACGCAGGTGCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCgg                                                                     <  2:13829485/94‑1 (MQ=255)

                        cATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGc                                                                    <  1:6753537/96‑1 (MQ=255)

                        cATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGc                                                                    <  2:4847915/96‑1 (MQ=255)

                         aTTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCa                                                                   >  1:1129226/1‑96 (MQ=255)

                          tttAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGATGCGGTCATTGCTCCCACCTCTTTTTATCGGCAt                                                                  <  2:6661615/96‑1 (MQ=255)

                          tttAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCAt                                                                  <  2:13095260/96‑1 (MQ=255)

                          tttAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCAt                                                                  >  2:13384880/1‑96 (MQ=255)

                          tttAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCAt                                                                  <  1:10354174/96‑1 (MQ=255)

                            tAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCAttt                                                                <  2:10486638/96‑1 (MQ=255)

                            tAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCCCTTTTTATCGGCAttt                                                                <  2:10877/96‑1 (MQ=255)

                             aGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTc                                                               <  1:2520076/96‑1 (MQ=255)

                              gCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTcc                                                              <  1:8096550/96‑1 (MQ=255)

                               ccAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCcg                                                             >  2:14308921/1‑96 (MQ=255)

                               ccAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCcg                                                             <  2:11122401/96‑1 (MQ=255)

                                cAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCcgc                                                            <  2:3015138/96‑1 (MQ=255)

                                 aaTTACTTGCTGAACGCACCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCcgcg                                                           <  1:3871243/96‑1 (MQ=255)

                                 aaTTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCTGTCATTGCTCCCACCTCTTTTTATCGGCATTTCcgcg                                                           >  2:4513492/1‑96 (MQ=255)

                                 aaTTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTCGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCcgcg                                                           <  1:2958437/96‑1 (MQ=255)

                                  aTTAAGGGCCGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGa                                                          <  2:5906829/96‑1 (MQ=255)

                                    tAAGGGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACg                                                        >  1:2118891/1‑96 (MQ=255)

                                         gcggAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTAATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGAc                                                   <  2:1593838/93‑1 (MQ=255)

                                         gCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGAc                                                   <  2:7110684/96‑1 (MQ=255)

                                         gCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGAc                                                   >  2:1172267/1‑96 (MQ=255)

                                            gAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGaa                                                >  1:5425662/1‑96 (MQ=255)

                                            gAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGGAGACGaa                                                >  2:9516018/1‑96 (MQ=255)

                                             aaCGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAAc                                               >  2:10102148/1‑96 (MQ=255)

                                             aaCGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAAc                                               >  2:8165603/1‑96 (MQ=255)

                                             aaCGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCGCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGTCGAAc                                               >  1:4696913/1‑96 (MQ=255)

                                              aCGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCCCCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACt                                              <  2:3401206/96‑1 (MQ=255)

                                                gCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTgg                                            >  1:2708287/1‑96 (MQ=255)

                                                  aGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGt                                          >  1:6189599/1‑96 (MQ=255)

                                                   gCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTc                                         <  2:7357283/96‑1 (MQ=255)

                                                   gCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTc                                         <  1:6940858/96‑1 (MQ=255)

                                                   gCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTc                                         <  2:6327591/96‑1 (MQ=255)

                                                   gCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTc                                         >  1:14113645/1‑96 (MQ=255)

                                                    cTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGttt                                        <  1:10816443/96‑3 (MQ=255)

                                                       ctgcAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGAc                                     <  1:12690277/93‑1 (MQ=255)

                                                       cGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGAc                                     <  2:11464689/96‑1 (MQ=255)

                                                        gCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGAcc                                    >  1:4233713/1‑96 (MQ=255)

                                                        gCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTATGAcc                                    >  1:6511022/1‑96 (MQ=255)

                                                           atccTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATg                                 >  1:11047893/3‑96 (MQ=255)

                                                             ccTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGt                               <  1:3274450/96‑1 (MQ=255)

                                                               tGAGTCTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTtg                             <  2:12733419/96‑1 (MQ=255)

                                                                gAGTTTGCGTGAAGTGGCGCGTGAAGCGGTTATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTtga                            >  1:4573005/1‑96 (MQ=255)

                                                                gAGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTtga                            <  2:2240185/96‑1 (MQ=255)

                                                                 aGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTtgat                           <  2:974043/96‑1 (MQ=255)

                                                                 aGTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTtgat                           >  1:12155198/1‑96 (MQ=255)

                                                                 aGTTTAAGTGAAGTGGCGCGTGAAGCGGTCACTGCTCCCCCCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTtgat                           <  1:5695374/96‑1 (MQ=255)

                                                                  gTTTGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTtgatg                          <  2:8360976/96‑1 (MQ=255)

                                                                    ttGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATgag                        >  2:2597798/1‑96 (MQ=255)

                                                                    ttGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATgag                        >  1:8423095/1‑96 (MQ=255)

                                                                     tGCGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATgaga                       <  1:10007955/96‑1 (MQ=255)

                                                                       cGTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGc                     <  1:5994047/96‑1 (MQ=255)

                                                                        gTGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCg                    >  2:869197/1‑96 (MQ=255)

                                                                         tGAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCgg                   <  1:12487450/96‑1 (MQ=255)

                                                                          gAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGt                  >  1:7835818/1‑96 (MQ=255)

                                                                          gAAGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGCTGATGAGAGCGGt                  >  2:3573118/1‑96 (MQ=255)

                                                                           aaGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGtt                 >  2:8819722/1‑96 (MQ=255)

                                                                            aGTGGCGCGTGAAGCGGTCATTGCTTCCACCTCTTTTTATCGGCATTTCCGCGTCGTAGACGAACTGGGTCTGACCATGGTTTATGTGAGTGtttt                >  1:3757314/1‑96 (MQ=255)

                                                                            aGTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGCGTCTGACCATGGTTGATGAGAGCGGttt                >  1:13805432/1‑96 (MQ=255)

                                                                             gTGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTa               <  2:5348286/96‑1 (MQ=255)

                                                                              tGGCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTaa              >  1:5038844/1‑96 (MQ=255)

                                                                               ggCGCGTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAAt             >  1:949753/1‑96 (MQ=255)

                                                                                  gcgTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCt          <  1:6713484/96‑1 (MQ=255)

                                                                                   cgcgAAGCGGTCATTCCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTCATGCTa         <  2:3883177/93‑1 (MQ=255)

                                                                                   cgTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCtg         <  1:13768223/96‑2 (MQ=255)

                                                                                    gTGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTAc        >  2:93989/1‑96 (MQ=255)

                                                                                     tGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACg       >  1:1053829/1‑96 (MQ=255)

                                                                                     tGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACg       >  2:7903178/1‑96 (MQ=255)

                                                                                     tGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACg       >  2:13596664/1‑96 (MQ=255)

                                                                                     tGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACg       >  2:4249113/1‑96 (MQ=255)

                                                                                     tGAAGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACg       <  1:7200182/96‑1 (MQ=255)

                                                                                        aGCGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCa    >  2:6130012/1‑96 (MQ=255)

                                                                                          cGGTCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAAc  <  2:12917104/96‑1 (MQ=255)

CCCGCCGTTCGCTGGTGGAAGCCGCATTTAGCCAATTAAGTGCTGAACGCAGCTTCGCCAGCCTGAGTTTGCGTGAAGTGGCGCGTGAAGCGGGCATTGCTCCCACCTCTTTTTATCGGCATTTCCGCGACGTAGACGAACTGGGTCTGACCATGGTTGATGAGAGCGGTTTAATGCTACGCCAAC  >  NC_000913/4158734‑4158919

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`