BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,842,033	G→T	R67R (CGC→CGA)	hycG ←	hydrogenase activity

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,842,033	0	G	T	99.6%	2080.9 / NA	533	R67R (CGC→CGA)	hycG	hydrogenase activity
Reads supporting (aligned to +/- strand): ref base G (1/1); new base T (263/268); total (264/269)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 549 total aligned reads displayed.

ACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGA  >  NC_000913/2841940‑2842125

 cgcagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGt                                                                                           <  2:9556533/96‑1 (MQ=255)

 cgcagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGt                                                                                           >  1:11743613/1‑96 (MQ=255)

  gcagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGtt                                                                                          >  2:10763271/1‑96 (MQ=255)

  gcagcgcagGGGATCGCATTGCACGGGTGACCGCGCAGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGtt                                                                                          <  1:4657764/96‑1 (MQ=255)

   cagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTc                                                                                         >  1:13801930/1‑96 (MQ=255)

   cagcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGGTc                                                                                         >  1:3734752/1‑96 (MQ=255)

    agcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCt                                                                                        <  1:51060/96‑1 (MQ=255)

    agcgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCt                                                                                        >  1:6603800/1‑96 (MQ=255)

      cgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCtcc                                                                                      <  1:11495432/96‑3 (MQ=255)

      cgcagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGc                                                                                      >  1:13119261/1‑96 (MQ=255)

      cgcagGGGATCGCATTGCACGGGTGACCGCGCCGGGAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGc                                                                                      >  2:11101244/1‑96 (MQ=255)

        cagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAATGAACGACTTTAATGCCGAATCGTTCTGCAt                                                                                    >  2:11649598/1‑96 (MQ=255)

        cagGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCAt                                                                                    >  2:4440335/1‑96 (MQ=255)

         agGGGATCGCATTGCACGGGGGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATc                                                                                   >  2:3414237/1‑96 (MQ=255)

          ggggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCa                                                                                  >  1:11236003/1‑96 (MQ=255)

           gggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCaa                                                                                 >  1:5446577/1‑96 (MQ=255)

           gagATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCaa                                                                                 >  1:11116497/3‑96 (MQ=255)

            ggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCaaa                                                                                <  2:13729622/96‑1 (MQ=255)

            ggATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCaaa                                                                                >  2:10972099/1‑96 (MQ=255)

             gATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAAc                                                                               >  2:4174830/1‑96 (MQ=255)

             gATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAAc                                                                               <  2:6171915/96‑1 (MQ=255)

              aTCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACa                                                                              >  2:3471536/1‑96 (MQ=255)

               tCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAg                                                                             >  2:5912488/1‑96 (MQ=255)

                cGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgc                                                                            <  2:5005505/96‑1 (MQ=255)

                cGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgc                                                                            <  2:3106561/96‑1 (MQ=255)

                  cATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcgg                                                                          <  2:4880813/96‑1 (MQ=255)

                  cATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcgg                                                                          <  2:12607628/96‑1 (MQ=255)

                   aTTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAgcggc                                                                         >  1:9126538/1‑96 (MQ=255)

                     tGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGa                                                                       <  2:10263283/96‑1 (MQ=255)

                       cACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGaaa                                                                     >  1:4299630/1‑96 (MQ=255)

                         cGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGc                                                                   <  1:7467605/96‑1 (MQ=255)

                         cGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGc                                                                   <  1:4491098/96‑1 (MQ=255)

                         cGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGc                                                                   >  1:5290585/1‑96 (MQ=255)

                          gggTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCg                                                                  >  1:833854/1‑96 (MQ=255)

                           ggTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGt                                                                 <  1:12174457/96‑1 (MQ=255)

                            gTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTg                                                                <  1:2211986/96‑1 (MQ=255)

                            gTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTg                                                                <  2:904655/96‑1 (MQ=255)

                              gACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGc                                                              <  2:5779975/96‑1 (MQ=255)

                                 cgcgCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAACCAGCGGCGAAAGCGTGGCGaa                                                           <  1:1024962/96‑1 (MQ=255)

                                 cgcgCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaa                                                           <  1:12826017/96‑1 (MQ=255)

                                  gcgcCGGTAAACAGTAAAATATCCGCATGAGGCGGTGAAGGAACGACTTTAATGCCGAATCGATCTGCATCAAACAGCGGCGAAAGCGTGGCGaaa                                                          <  2:14089939/96‑1 (MQ=255)

                                  gcgcCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGaaa                                                          <  2:9667421/96‑1 (MQ=255)

                                    gcCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAACGCGTGGCGAAAat                                                        <  1:10972099/96‑1 (MQ=255)

                                      cGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAattt                                                      <  2:7440108/96‑1 (MQ=255)

                                        gTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcg                                                    <  2:5410333/96‑1 (MQ=255)

                                         tAAACAGTAAAATATGCGTATGACGCGGAGAAGGAACGACTTTCATGCCGAATCGTTCTGCATCCAACAGCGGCTAATGCGTGGGGTAAatttcgc                                                   >  1:9874313/1‑95 (MQ=255)

                                         tAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcga                                                   >  1:11902618/1‑96 (MQ=255)

                                          aaaCAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgat                                                  >  1:7620334/1‑96 (MQ=255)

                                           aaCAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgatt                                                 <  1:5628147/96‑1 (MQ=255)

                                            aCAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGACAatttcgattt                                                >  2:12817008/1‑96 (MQ=255)

                                            aCAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgattt                                                <  2:9312637/96‑1 (MQ=255)

                                             cAGTAGAAGATCCGCATGACGCGGTGAAGGAACGACTTTTATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgattcc                                               <  1:13609891/96‑3 (MQ=255)

                                             cAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgatttc                                               <  1:2530276/96‑1 (MQ=255)

                                              aGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAatttcgatttcg                                              >  1:3873515/1‑96 (MQ=255)

                                                tAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCa                                            >  2:13905371/1‑96 (MQ=255)

                                                 aaaaTATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCaa                                           <  2:6508860/96‑1 (MQ=255)

                                                  aaaTATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAc                                          <  2:12280183/96‑1 (MQ=255)

                                                   actatCCGCATGACGCGGTGAAGGAACGACTATAATGCCGAGTCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAcc                                         <  1:9995277/94‑1 (MQ=255)

                                                   aaTATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAAcc                                         <  2:11379937/96‑1 (MQ=255)

                                                      atCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCACACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGtt                                      <  1:14199422/96‑1 (MQ=255)

                                                       tCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTg                                     <  1:4006183/96‑1 (MQ=255)

                                                       tCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTg                                     <  1:8856275/96‑1 (MQ=255)

                                                        ccGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGc                                    >  1:12707657/1‑96 (MQ=255)

                                                         cGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTgga                                   >  1:10417049/1‑94 (MQ=255)

                                                         cGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCa                                   <  2:9750762/96‑1 (MQ=255)

                                                         cGCATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCa                                   <  1:9639650/96‑1 (MQ=255)

                                                           cATGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgc                                 <  2:8763899/96‑1 (MQ=255)

                                                            aTGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgcc                                <  1:3029254/96‑1 (MQ=255)

                                                             tGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccg                               >  2:692422/1‑96 (MQ=255)

                                                             tGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAgccg                               >  1:13234263/1‑96 (MQ=255)

                                                             tGACGCGGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGGGGCGAAAATTTCGATTTCGCAACCGTTGCAgccg                               >  2:7943375/1‑96 (MQ=255)

                                                                 gcgGTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCcgccgc                           <  2:12978093/96‑1 (MQ=255)

                                                                   ggTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCGGCAg                         >  1:6067045/1‑96 (MQ=255)

                                                                   ggTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAg                         >  1:7895136/1‑96 (MQ=255)

                                                                   ggTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAg                         >  2:8488570/1‑96 (MQ=255)

                                                                    gTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGt                        >  2:5253477/1‑96 (MQ=255)

                                                                    gTGAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGt                        <  1:7232914/96‑1 (MQ=255)

                                                                    gTGAAGGAACGACTTTAATACCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGt                        >  2:12100068/1‑96 (MQ=255)

                                                                      gAAGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTcc                      >  2:3780125/1‑96 (MQ=255)

                                                                       aaGGAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCa                     >  2:14363481/1‑96 (MQ=255)

                                                                         ggAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcg                   <  2:4548573/96‑1 (MQ=255)

                                                                         ggAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcg                   <  1:10040564/96‑1 (MQ=255)

                                                                         ggAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcg                   <  2:259798/96‑1 (MQ=255)

                                                                          gAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcgc                  <  1:8627598/96‑1 (MQ=255)

                                                                          gAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcgc                  >  1:3377844/1‑96 (MQ=255)

                                                                          gAACGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCAcgc                  <  2:1781369/96‑1 (MQ=255)

                                                                             cGACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGt               >  2:8260125/1‑96 (MQ=255)

                                                                              gACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTa              >  2:8611148/1‑96 (MQ=255)

                                                                              gACTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTa              <  1:3445894/96‑1 (MQ=255)

                                                                               aCTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTaa             >  1:706468/1‑96 (MQ=255)

                                                                                cTTTAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTaaa            <  2:7741675/96‑1 (MQ=255)

                                                                                cTTTAATGCCGAATCGTTCTGCATCAAACAGCGACGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTTCAGCCGCCGCAGTCCACGCGGTaaa            >  2:6643713/1‑96 (MQ=255)

                                                                                 tttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCACCCGTTGCAGCCGCCGCAGTCCACGCGGTAAAc           <  1:1913738/96‑1 (MQ=255)

                                                                                  ttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACa          >  2:13377229/1‑96 (MQ=255)

                                                                                  ttAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACa          <  1:1629539/96‑1 (MQ=255)

                                                                                   tAATGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACAt         <  2:3230211/96‑1 (MQ=255)

                                                                                    attGCCGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATa        <  1:14423569/94‑1 (MQ=255)

                                                                                         cGAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAg   <  1:6442466/96‑1 (MQ=255)

                                                                                          gAATCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTCCGCAACCGTTGCAGCCGCCGCCACCACCCCGGTAAACATAGGCAGa  >  1:12569858/1‑96 (MQ=255)

                                                                                          gAATCGTTCTGCATCAAACAGCGGCGAAAGCGGGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGa  >  1:5952902/1‑96 (MQ=255)

ACGCAGCGCAGGGGATCGCATTGCACGGGTGACCGCGCCGGTAAACAGTAAAATATCCGCATGACGCGGTGAAGGAACGACTTTAATGCCGAAGCGTTCTGCATCAAACAGCGGCGAAAGCGTGGCGAAAATTTCGATTTCGCAACCGTTGCAGCCGCCGCAGTCCACGCGGTAAACATAGGCAGA  >  NC_000913/2841940‑2842125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 17 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`