BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,077,905	A→G	S584P (TCG→CCG)	tktA ←	transketolase 1 isozyme

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,077,905	0	A	G	99.8%	2198.6 / NA	570	S584P (TCG→CCG)	tktA	transketolase 1 isozyme
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (280/289); minor base T (0/1); total (280/290)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 610 total aligned reads displayed.

TACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGACATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAA  >  NC_000913/3077812‑3077997

 aCCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCAt                                                                                           <  1:1514177/96‑1 (MQ=255)

  cccGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATg                                                                                          <  2:8602206/96‑1 (MQ=255)

    cGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGa                                                                                        >  2:10196523/1‑96 (MQ=255)

     gCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACTGCATGGac                                                                                       >  2:9374040/1‑96 (MQ=255)

     gCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGac                                                                                       >  1:1727422/1‑96 (MQ=255)

     gCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGac                                                                                       >  1:7631108/1‑96 (MQ=255)

      cTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTCAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGaca                                                                                      >  2:12387422/1‑96 (MQ=255)

      cTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGaca                                                                                      <  1:4024911/96‑1 (MQ=255)

      cTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGaca                                                                                      >  2:3647224/1‑96 (MQ=255)

       ttCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGacac                                                                                     >  1:13752102/1‑96 (MQ=255)

       ttCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGacac                                                                                     >  1:11171205/1‑96 (MQ=255)

        tCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcacc                                                                                    <  2:13464249/96‑1 (MQ=255)

         cTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcacca                                                                                   >  2:11469847/1‑96 (MQ=255)

         cTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcacca                                                                                   >  1:8692117/1‑96 (MQ=255)

         cTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcacca                                                                                   >  2:13301847/1‑96 (MQ=255)

         cTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcacca                                                                                   <  2:1855865/96‑1 (MQ=255)

          tACAGCACCGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcaccac                                                                                  <  1:14104098/96‑1 (MQ=255)

          tACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcaccac                                                                                  <  2:3397896/96‑1 (MQ=255)

          tACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcaccac                                                                                  <  1:9279209/96‑1 (MQ=255)

          tACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcaccac                                                                                  <  2:4308467/96‑1 (MQ=255)

           aCAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGAcaccatg                                                                                 <  1:10621296/96‑3 (MQ=255)

           aCAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcg                                                                                 <  1:7403416/96‑1 (MQ=255)

           aCAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcg                                                                                 <  1:5284649/96‑1 (MQ=255)

              gCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCAcgcgc                                                                              <  1:1931619/96‑1 (MQ=255)

                aaCGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACgcgcgc                                                                            >  2:9655531/1‑96 (MQ=255)

                 aCGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCt                                                                           >  1:6081318/1‑96 (MQ=255)

                  cgcgTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCtt                                                                          <  1:1095093/96‑1 (MQ=255)

                   gcgTGCAGTCACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCttt                                                                         <  1:8273501/96‑1 (MQ=255)

                    cgTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTc                                                                        >  1:9485258/1‑96 (MQ=255)

                          gTAACCGCTTTCGGCACTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCt                                                                  <  1:10352969/96‑1 (MQ=255)

                            aaCCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTc                                                                <  1:5510818/96‑1 (MQ=255)

                             aCCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCg                                                               <  1:9714714/96‑1 (MQ=255)

                             aCCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCg                                                               >  1:8925195/1‑96 (MQ=255)

                              ccGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCgg                                                              <  2:8981564/96‑1 (MQ=255)

                               cGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGc                                                             <  2:2053331/96‑1 (MQ=255)

                               cGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGc                                                             <  1:3697901/96‑1 (MQ=255)

                                gCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGca                                                            >  2:1643255/1‑96 (MQ=255)

                                gCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGca                                                            >  2:2453060/1‑96 (MQ=255)

                                 cTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCCTGGACACCACGCGCGCTTTCACGCCTTCGGcag                                                           <  1:11933654/96‑1 (MQ=255)

                                  tttCGGCAGTACGGATTCACGGTAAGCAGCGTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagt                                                          <  1:12749892/96‑1 (MQ=255)

                                     cGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcag                                                       <  2:14294881/96‑1 (MQ=255)

                                     cGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcag                                                       <  1:4399148/96‑1 (MQ=255)

                                     cGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcag                                                       >  2:13107312/1‑96 (MQ=255)

                                      ggCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcagt                                                      <  2:6081318/96‑1 (MQ=255)

                                      ggCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcagt                                                      >  2:7279016/1‑96 (MQ=255)

                                      ggCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcaat                                                      <  1:14275217/96‑3 (MQ=255)

                                      ggCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCATCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGcagtcagt                                                      >  1:13034352/1‑96 (MQ=255)

                                       gCAGTCCGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGtt                                                     <  1:2549197/96‑1 (MQ=255)

                                        ccgTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCCCGCCTTCGGCAGTCAGttt                                                    <  1:5443014/94‑1 (MQ=255)

                                        cAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGttt                                                    >  1:4905011/1‑96 (MQ=255)

                                         aGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGtttt                                                   >  2:8237945/1‑96 (MQ=255)

                                         aGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGtttt                                                   >  1:6818035/1‑96 (MQ=255)

                                          ttACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGttttt                                                  >  1:11348790/2‑96 (MQ=255)

                                          gTACGGATTCACGGTAAGCGGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGttttt                                                  >  2:12105330/1‑96 (MQ=255)

                                          gTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGttttt                                                  >  1:9900721/1‑96 (MQ=255)

                                           tACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTc                                                 >  1:7946524/1‑96 (MQ=255)

                                            aCGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCg                                                >  1:9044603/1‑96 (MQ=255)

                                             cGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGt                                               >  2:483419/1‑96 (MQ=255)

                                             cGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGt                                               >  1:6512332/1‑96 (MQ=255)

                                               gATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAg                                             >  1:3423676/1‑96 (MQ=255)

                                               gATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAg                                             <  1:10421738/96‑1 (MQ=255)

                                                 ttCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgc                                           <  2:6240158/96‑1 (MQ=255)

                                                 ttCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgc                                           <  2:6818035/96‑1 (MQ=255)

                                                 ttCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgc                                           >  1:13391143/1‑96 (MQ=255)

                                                  tCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgca                                          <  2:3797024/96‑1 (MQ=255)

                                                  tCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgca                                          <  2:5437551/96‑1 (MQ=255)

                                                  tCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCCTCGGCAGTCAGTTTTTCGTAGgca                                          >  2:10877553/1‑96 (MQ=255)

                                                   cACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgcag                                         <  2:11196256/96‑1 (MQ=255)

                                                   cACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgcag                                         >  2:13743688/1‑96 (MQ=255)

                                                    aCGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACATCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgcagc                                        <  2:12886711/96‑1 (MQ=255)

                                                    aCGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgcagc                                        >  1:7040675/1‑96 (MQ=255)

                                                     cGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgcagca                                       >  2:12638937/1‑96 (MQ=255)

                                                     cGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGgcaaca                                       >  1:13621952/1‑96 (MQ=255)

                                                      ggTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCaa                                      <  2:5022040/96‑1 (MQ=255)

                                                       gTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAAc                                     >  2:9183340/1‑96 (MQ=255)

                                                       gTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAAc                                     >  2:5258073/1‑96 (MQ=255)

                                                        tAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACa                                    <  1:7540973/96‑1 (MQ=255)

                                                         aaGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAg                                   >  1:3019514/1‑96 (MQ=255)

                                                           gcagcaTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGcc                                 <  2:777152/96‑1 (MQ=255)

                                                           gcagcaTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGcc                                 <  2:6565061/96‑1 (MQ=255)

                                                            cagcaTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCa                                <  1:5088378/96‑1 (MQ=255)

                                                              gcaTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGt                              <  2:41192/96‑1 (MQ=255)

                                                               caTCCTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTGCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGtt                             <  2:2807501/96‑1 (MQ=255)

                                                                  ccTGCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCaa                          >  2:10619770/1‑96 (MQ=255)

                                                                     gCTTGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACtt                       <  2:3072153/96‑1 (MQ=255)

                                                                      cTTGTCAAATGCGGCGGTAGACGGCATGGACACCACGCGCGCTTTCACTCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTc                      <  1:12917782/96‑1 (MQ=255)

                                                                       ttGTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGcc                                 <  2:11803479/84‑1 (MQ=255)

                                                                       ttGTCAAATGCGCCGGTAGCCGGAATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCt                     <  1:7341679/96‑1 (MQ=255)

                                                                         ggcAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGa                   >  1:11525916/3‑96 (MQ=255)

                                                                         gTCAAATGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGa                   <  2:5879771/96‑1 (MQ=255)

                                                                               tGCGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGt             >  2:14020945/1‑96 (MQ=255)

                                                                               tGCGTCGGTAGACGGCATGGACAACACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGt             <  1:12425245/96‑1 (MQ=255)

                                                                                gCGTCGGTAGACGGCATGGACACAACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTa            <  1:10186039/96‑1 (MQ=255)

                                                                                 cGTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAg           <  1:1164490/96‑1 (MQ=255)

                                                                                  gTCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGc          <  2:8775614/96‑1 (MQ=255)

                                                                                   tCGGTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCg         <  1:1327685/96‑1 (MQ=255)

                                                                                   tCGGTAGACGGCATGGACACCACGCGCGCTTCCACTCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCg         <  2:7597759/96‑1 (MQ=255)

                                                                                     ggTAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCGACTTCTGAACCGGTAGCGAt       >  1:8383148/1‑96 (MQ=255)

                                                                                       tAGACGGCATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGa     >  1:5779237/1‑96 (MQ=255)

                                                                                         gacggcATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGaaa   <  1:12193574/96‑1 (MQ=255)

TACCCGCTTCTACAGCAACGCGTGCAGTAACCGCTTTCGGCAGTACGGATTCACGGTAAGCAGCATCCTGCTTGTCAAATGCGTCGGTAGACGACATGGACACCACGCGCGCTTTCACGCCTTCGGCAGTCAGTTTTTCGTAGGCAGCAACAGCCAGTTCAACTTCTGAACCGGTAGCGATGAAAA  >  NC_000913/3077812‑3077997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`