Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 509,606 (T)6→5 coding (998/2505 nt) ybaR ← putative ATPase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913509,6010T.99.3% 1345.0 / ‑0.1 295coding (1003/2505 nt)ybaRputative ATPase
Reads supporting (aligned to +/- strand):  ref base T (0/1);  major base . (133/158);  minor base A (2/0);  total (135/160)
Fisher's exact test for biased strand distribution p-value = 2.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 319 total aligned reads displayed.

TTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCG  >  NC_000913/509509‑509689
                                                                                            |                                                                                        
ttCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCt                                                                                          <  1:6994577/93‑1 (MQ=255)
ttCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCt                                                                                          <  1:3900974/93‑1 (MQ=255)
ttCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCt                                                                                          <  1:1479444/93‑1 (MQ=255)
  cGCCATGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATGCCTGGCTGCACTTCTGCCAGAGGCACGCttt                                                                                        >  2:2968666/1‑93 (MQ=255)
   gCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCtttt                                                                                       <  1:6153564/93‑1 (MQ=255)
        gggTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCtttttcacc                                                                                  >  1:5267717/1‑89 (MQ=255)
         ggTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATCCCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCt                                                                                <  1:8273175/93‑1 (MQ=255)
         ggTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCt                                                                                <  2:7011935/93‑1 (MQ=255)
             aTCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGt                                                                            <  2:3433979/93‑1 (MQ=255)
              actcGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTc                                                                           <  1:2289049/92‑1 (MQ=255)
                 cGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGt                                                                        <  1:2186688/93‑1 (MQ=255)
                  gCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTa                                                                       <  2:7399411/93‑1 (MQ=255)
                  gCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTa                                                                       <  2:7756092/93‑1 (MQ=255)
                  gCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTa                                                                       >  2:6842181/1‑93 (MQ=255)
                    cATCTACCGGCACGCGACCGCCGGTCGTCAGGCGCAGCAACATCCCTGGCTGCACTTCTGCCAGAGGCACTC‑TTTTTCACCTTCGTCAGTAAc                                                                     <  1:3605102/93‑1 (MQ=255)
                     aTCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAAcc                                                                    <  2:3759581/93‑1 (MQ=255)
                     aTCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAAcc                                                                    >  1:5492253/1‑93 (MQ=255)
                      tCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCa                                                                   >  1:3160255/1‑93 (MQ=255)
                      tCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCa                                                                   <  2:6070318/93‑1 (MQ=255)
                      tCTACCGGCACCCGATCGCCGGTCGTCAGTCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCa                                                                   <  2:1405077/93‑1 (MQ=255)
                       ctcccGACACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAg                                                                  <  2:3184877/90‑1 (MQ=255)
                         aCCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGc                                                                >  2:932521/1‑93 (MQ=255)
                          ccGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCg                                                               <  2:8160274/93‑1 (MQ=255)
                           cGGCACGCGATCTCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGt                                                              <  2:4293012/93‑1 (MQ=255)
                             gCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGc                                                            <  1:97459/93‑1 (MQ=255)
                                 gcgATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTc                                                        >  2:6769233/1‑93 (MQ=255)
                                  cgATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTcg                                                       >  1:4343521/1‑93 (MQ=255)
                                  cgATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTcg                                                       <  2:3647629/93‑1 (MQ=255)
                                   gATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTcgg                                                      <  1:4739889/93‑1 (MQ=255)
                                    aTCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTcggc                                                     <  1:6406348/93‑1 (MQ=255)
                                    aTCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTcggc                                                     >  1:6755579/1‑93 (MQ=255)
                                     tCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTcggcg                                                    <  2:7815085/93‑1 (MQ=255)
                                      cGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTcggcgg                                                   <  2:3376810/93‑1 (MQ=255)
                                       gCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCggg                                                  >  2:5704213/1‑93 (MQ=255)
                                        ccGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCgggg                                                 <  2:5956008/93‑1 (MQ=255)
                                         cGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGt                                                <  1:3014650/93‑1 (MQ=255)
                                         cGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGt                                                <  1:8045604/93‑1 (MQ=255)
                                         cGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGt                                                >  2:5194880/1‑93 (MQ=255)
                                          ggTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCgggggt                                               >  2:5387665/1‑91 (MQ=255)
                                          ggTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGtt                                               <  2:7205206/93‑1 (MQ=255)
                                           gtcgtcAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTa                                              >  2:1252128/1‑93 (MQ=255)
                                           gtcgtcAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTCCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTa                                              >  2:2756893/1‑93 (MQ=255)
                                             cgtcAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTaaa                                            <  1:2759821/93‑1 (MQ=255)
                                              gtcAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAAt                                           >  1:7073896/1‑93 (MQ=255)
                                              gtcAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAAt                                           >  1:1380155/1‑93 (MQ=255)
                                               tcAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATc                                          <  2:651319/93‑1 (MQ=255)
                                               tcAGGCGCAGCAACATACCAGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGGTAAATc                                          >  1:8536979/1‑93 (MQ=255)
                                                 aGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGa                                        <  2:8308003/93‑1 (MQ=255)
                                                 aGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGa                                        <  2:8452967/93‑1 (MQ=255)
                                                  ggCGCAGCAACATACCTGGCTGCCCTTCTGCAAGAGGCACGC‑TTTTTCCCCTTCGTTAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAg                                       <  2:1901823/93‑1 (MQ=255)
                                                   gcgcAGCAACATTCCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGt                                      <  2:159881/93‑1 (MQ=255)
                                                    cgcAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTa                                     <  2:5544681/93‑1 (MQ=255)
                                                     gcagcaACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGGGTac                                    >  2:264653/1‑92 (MQ=255)
                                                     gcagcaACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTaa                                    >  1:6858928/1‑93 (MQ=255)
                                                     gcagcaACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTaa                                    >  1:255885/1‑93 (MQ=255)
                                                     gcagcaACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGGTAAATCGAGTaa                                    >  2:4755226/1‑93 (MQ=255)
                                                      cagcaACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAAc                                   <  1:793011/93‑1 (MQ=255)
                                                      cagcaACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAAc                                   <  2:2276357/93‑1 (MQ=255)
                                                        gcaACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACtt                                 <  2:7329675/93‑1 (MQ=255)
                                                        gcaACATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACtt                                 >  1:1863615/1‑93 (MQ=255)
                                                           aCATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTc                              <  2:6945799/93‑1 (MQ=255)
                                                           aCATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGGTAAATCGAGTAACTTTTc                              >  2:6273720/1‑93 (MQ=255)
                                                           aCATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGGTAAATCGAGTAACTTTTc                              >  1:4067332/1‑93 (MQ=255)
                                                            cATCCCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTcc                             <  1:285078/93‑1 (MQ=255)
                                                            cATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTcc                             <  1:5704889/93‑1 (MQ=255)
                                                            cATACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTcc                             <  2:7533565/93‑1 (MQ=255)
                                                             aTACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCa                            <  1:3459657/93‑1 (MQ=255)
                                                              tACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAg                           >  2:2080639/1‑93 (MQ=255)
                                                              tACCTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAg                           >  1:5909767/1‑93 (MQ=255)
                                                               aCCTGGCTTCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTGAGAGCGAGCAACAGTTCCAgc                          >  2:5798314/1‑93 (MQ=255)
                                                                ccTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAgcg                         >  1:4537083/1‑93 (MQ=255)
                                                                 cTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAgcgc                        >  2:4378087/1‑93 (MQ=255)
                                                                 cTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAgcgc                        >  1:4503782/1‑93 (MQ=255)
                                                                 cTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAgcgc                        <  1:8801643/93‑1 (MQ=255)
                                                                 cTGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGGTAAATCGAGTAACTTTTCCAgcgc                        >  1:1672913/1‑93 (MQ=255)
                                                                  tGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCCGCGcc                       >  1:6499940/1‑93 (MQ=255)
                                                                  tGGCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGcc                       <  2:3952567/93‑1 (MQ=255)
                                                                   ggCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCt                      <  2:3102808/93‑1 (MQ=255)
                                                                    gCTGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCtt                     <  2:1668359/93‑1 (MQ=255)
                                                                      tGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCCGCGCCTTag                   >  2:2609224/1‑93 (MQ=255)
                                                                      tGCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGGTAAATCGAGTAACTTTTCCAGCGCCtttg                   >  2:3283926/1‑91 (MQ=255)
                                                                       gCACTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTaga                  >  2:1171473/1‑93 (MQ=255)
                                                                        taCTTCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTagaa                 <  2:7645300/92‑1 (MQ=255)
                                                                            tCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAAc             >  1:1254418/1‑93 (MQ=255)
                                                                            tCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAAc             <  2:1108857/93‑1 (MQ=255)
                                                                            tCTGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAAc             <  1:1032257/93‑1 (MQ=255)
                                                                              tGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGc           >  1:20104/1‑93 (MQ=255)
                                                                              tGCCAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGc           <  2:2426008/93‑1 (MQ=255)
                                                                                ccAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTg         >  2:4457768/1‑93 (MQ=255)
                                                                                 cAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTgg        <  2:4648126/93‑1 (MQ=255)
                                                                                 cAGAGGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTgg        >  1:1035500/1‑93 (MQ=255)
                                                                                  agagGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGc       >  1:215278/1‑93 (MQ=255)
                                                                                   gagGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACCCTGGCg      <  2:8668677/93‑1 (MQ=255)
                                                                                    agGCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGt     <  1:3798893/93‑1 (MQ=255)
                                                                                     ggCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTg    >  2:2545470/1‑93 (MQ=255)
                                                                                      gCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTgc   <  1:7696511/93‑1 (MQ=255)
                                                                                      gCACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTgc   <  2:3878051/93‑1 (MQ=255)
                                                                                       cACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGTGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTgcg  <  1:3565204/93‑1 (MQ=255)
                                                                                       cACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCTTCGCCTTAGAAGAACGCTGTCGTgcg  >  2:6902548/1‑93 (MQ=255)
                                                                                       cACGC‑TTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTgcg  <  1:3309378/93‑1 (MQ=255)
                                                                                            |                                                                                        
TTCGCCCTGGGTAATCTCGCCATCTACCGGCACGCGATCGCCGGTCGTCAGGCGCAGCAACATACCTGGCTGCACTTCTGCCAGAGGCACGCTTTTTTCACCTTCGTCAGTAACCAGGCGTGCCGTCGGCGGGGTTAAATCGAGTAACTTTTCCAGCGCCTTAGAAGAACGCTGGCGTGCG  >  NC_000913/509509‑509689

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑