BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,392,651	(G)_5→4	coding (1008/1548 nt)	yjeF →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,392,647	0	G	.	92.4%	1126.2 / 76.3	290	coding (1004/1548 nt)	yjeF	hypothetical protein
Reads supporting (aligned to +/- strand): ref base G (13/9); new base . (119/147); total (134/156)
Fisher's exact test for biased strand distribution p-value = 2.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.50e-01

Only 100 of 335 total aligned reads displayed.

GATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCC  >  NC_000913/4392555‑4392735

gATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGGGTg                                                                                          >  1:6223996/1‑93 (MQ=255)

gATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTg                                                                                          <  1:438498/93‑1 (MQ=255)

gATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTg                                                                                          >  1:2125988/1‑93 (MQ=255)

 aTGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgg                                                                                         <  1:1785149/93‑1 (MQ=255)

 aTGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgg                                                                                         <  1:328533/93‑1 (MQ=255)

  tGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTggg                                                                                        <  2:329473/93‑1 (MQ=255)

  tGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTggg                                                                                        <  2:777552/93‑1 (MQ=255)

   ggTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgggg                                                                                       >  2:7605671/1‑93 (MQ=255)

   ggTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgggg                                                                                       >  2:1463790/1‑93 (MQ=255)

    gTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgggga                                                                                      >  1:5684239/1‑92 (MQ=255)

    gTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgggga                                                                                      >  2:6574878/1‑92 (MQ=255)

    gTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTgggga                                                                                      <  1:2943599/93‑2 (MQ=255)

      gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaa                                                                                    <  1:5029512/93‑1 (MQ=255)

      gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaa                                                                                    >  2:3652403/1‑93 (MQ=255)

      gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaa                                                                                    >  2:8753019/1‑93 (MQ=255)

      gCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaa                                                                                    >  2:6800636/1‑93 (MQ=255)

          gAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaaaaag                                                                                >  2:308980/1‑92 (MQ=255)

          gAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAg                                                                               <  1:1338612/93‑1 (MQ=255)

          gAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGCTCCCGGTCTGGGCCAGCAAGAGTGGGGAaagaag                                                                                >  2:1592723/1‑92 (MQ=255)

          gAACTGACGATGGACGCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGaaaaaag                                                                                <  1:7572162/93‑2 (MQ=255)

           aaCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGc                                                                              >  2:5906993/1‑93 (MQ=255)

           aaCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGc                                                                              <  1:6180193/93‑1 (MQ=255)

            aCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCa                                                                             <  1:8187645/93‑1 (MQ=255)

            aCTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCa                                                                             <  2:4055263/93‑1 (MQ=255)

             cagACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCAc                                                                            <  1:6835824/91‑1 (MQ=255)

              tGACGATGGACTCTCTTACCGAAAGCCTGGAAGGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACt                                                                           <  2:2928679/93‑1 (MQ=255)

                aCGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGc                                                                         >  1:3560583/1‑93 (MQ=255)

                  gATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTTCCGGTCTGGGCCAGCAAGAGT‑GGGGCAAAAAGCACTGCaa                                                                       >  2:2382527/1‑93 (MQ=255)

                    tGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaaa                                                                     >  1:8350889/1‑93 (MQ=255)

                    tGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaaa                                                                     <  2:346495/93‑1 (MQ=255)

                     ggACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaaaa                                                                    <  2:2659380/93‑1 (MQ=255)

                     ggACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCaaaaa                                                                    <  2:6717358/93‑1 (MQ=255)

                      gACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAg                                                                   <  1:424038/93‑1 (MQ=255)

                       aCTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGt                                                                  >  1:7937417/1‑93 (MQ=255)

                         tctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTg                                                                <  2:5676653/93‑1 (MQ=255)

                         tctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTg                                                                >  1:5189014/1‑93 (MQ=255)

                         tctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTg                                                                >  2:6178506/1‑93 (MQ=255)

                          ctctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTga                                                               >  1:6585758/1‑93 (MQ=255)

                           tctTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTgag                                                              >  2:3890880/1‑93 (MQ=255)

                             ttACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGaa                                                            >  1:7779937/1‑93 (MQ=255)

                              tACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAt                                                           >  2:4724814/1‑93 (MQ=255)

                              tACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAt                                                           >  2:3061236/1‑93 (MQ=255)

                                 cGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAAtttt                                                        >  1:7068945/1‑93 (MQ=255)

                                  gAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTc                                                       >  2:1927101/1‑93 (MQ=255)

                                  gAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTc                                                       >  2:8625856/1‑93 (MQ=255)

                                  gAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAACAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTc                                                       <  1:2334270/93‑1 (MQ=255)

                                   aaaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCg                                                      <  1:5131263/93‑1 (MQ=255)

                                    aaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGc                                                     >  1:3046474/1‑93 (MQ=255)

                                    aaGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGc                                                     >  1:2641557/1‑93 (MQ=255)

                                     aGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCa                                                    <  1:5837279/93‑1 (MQ=255)

                                      gCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCaa                                                   >  2:8025958/1‑93 (MQ=255)

                                        cTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAc                                                 <  1:8160874/93‑1 (MQ=255)

                                        cTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAAc                                                 >  2:2833216/1‑93 (MQ=255)

                                          ggAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCg                                               >  1:1486715/1‑93 (MQ=255)

                                             aTGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAACAGTTGAGAATTTTCGCAAACCGAtg                                            >  1:3689614/1‑93 (MQ=255)

                                              tGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgt                                           <  2:8221126/93‑1 (MQ=255)

                                              tGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgt                                           <  2:1865759/93‑1 (MQ=255)

                                               gggCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgtt                                          <  2:7238232/93‑1 (MQ=255)

                                                ggCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgttg                                         >  1:6361317/1‑93 (MQ=255)

                                                ggCCGATGTGGTGGTGAGTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGGtgttg                                         >  2:431528/1‑93 (MQ=255)

                                                 gtctaTGTGGTGGTGATTGGTCTCGGGCTGGGCCAGCAAGAGT‑GGTGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgttgt                                        <  2:4571702/89‑1 (MQ=255)

                                                 gCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgttgt                                        <  1:908590/93‑1 (MQ=255)

                                                 gCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGAtgttgg                                        >  1:2751691/1‑92 (MQ=255)

                                                  ccGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTtgtg                                       >  1:3481917/1‑93 (MQ=255)

                                                   cGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGCAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTgg                                      <  2:1022259/93‑1 (MQ=255)

                                                     aTGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                    >  2:8414791/1‑93 (MQ=255)

                                                     aTGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                    <  2:8145630/93‑1 (MQ=255)

                                                     aTGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGa                                    <  1:537660/93‑1 (MQ=255)

                                                      tgtgGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGAt                                   <  1:1664682/93‑1 (MQ=255)

                                                      tgtgGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGAt                                   <  1:7630291/93‑1 (MQ=255)

                                                       gtggtggtgATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATg                                  <  2:141009/93‑1 (MQ=255)

                                                           tggtgATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGa                              <  2:297275/93‑1 (MQ=255)

                                                            ggtgATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCGCTGCAAAAAGTTGAGAATTTGCGCAAACCGATGTTGTGGGATGCCGAt                             <  1:6878432/93‑1 (MQ=255)

                                                            ggtgATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGAt                             <  1:7749768/93‑1 (MQ=255)

                                                              tgATTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGc                           <  2:925746/93‑1 (MQ=255)

                                                                aTTGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGAAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCAt                         <  1:1835234/93‑1 (MQ=255)

                                                                aTTGATCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTGGAGAATTTTCGCAATCCGATGTTGTGGGATGCCGATGCAt                         <  2:5347473/93‑1 (MQ=255)

                                                                 ttGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCAtt                        >  2:7049736/1‑93 (MQ=255)

                                                                 ttGGTCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCAtt                        >  1:5617241/1‑93 (MQ=255)

                                                                     tCCCGGTCTGTGCCAGCAAGAGT‑GGGGAAAAAATCACTGCAAAAAGTTGAGAAATTTCCCAAACCGATGTTTTGGGATGCCGATGCATTGAAc                    <  2:7100723/93‑1 (MQ=255)

                                                                     tCCCGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAAc                    <  1:8496141/93‑1 (MQ=255)

                                                                       ccGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACct                  <  1:5281936/93‑1 (MQ=255)

                                                                       ccGGTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACct                  >  2:2049061/1‑93 (MQ=255)

                                                                         ggTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgc                <  1:1943948/93‑1 (MQ=255)

                                                                          gTCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgct               >  1:3039384/1‑93 (MQ=255)

                                                                           tCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgctg              >  2:5525661/1‑93 (MQ=255)

                                                                           tCTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACctgctg              >  1:4891702/1‑93 (MQ=255)

                                                                            cTGGGCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTgg             <  2:7873977/93‑1 (MQ=255)

                                                                              gggCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGACCCTGCTGGca           >  1:2209102/1‑93 (MQ=255)

                                                                                gCCAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaat         >  1:3954259/1‑93 (MQ=255)

                                                                                  cAGCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatca       >  1:8726307/1‑93 (MQ=255)

                                                                                    gCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcaat     >  2:5152459/1‑93 (MQ=255)

                                                                                    gCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcaat     >  2:3851512/1‑93 (MQ=255)

                                                                                    gCAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGcaatcaat     >  2:1206412/1‑93 (MQ=255)

                                                                                     cAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCaatcaatc    <  1:4501250/93‑1 (MQ=255)

                                                                                     cAAGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCaatcaatc    >  1:7264833/1‑93 (MQ=255)

                                                                                      aaGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATcc   >  2:2587304/1‑93 (MQ=255)

                                                                                      aaGAGT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTGCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATcc   <  1:107351/93‑1 (MQ=255)

                                                                                       agagT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATccc  >  2:7372778/1‑93 (MQ=255)

                                                                                       agagT‑GGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATccc  >  2:2811194/1‑93 (MQ=255)

GATGGTGCATGAACTGACGATGGACTCTCTTACCGAAAGCCTGGAATGGGCCGATGTGGTGGTGATTGGTCCCGGTCTGGGCCAGCAAGAGTGGGGGAAAAAAGCACTGCAAAAAGTTGAGAATTTTCGCAAACCGATGTTGTGGGATGCCGATGCATTGAACCTGCTGGCAATCAATCCC  >  NC_000913/4392555‑4392735

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`