BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,477,321	Δ1 bp	coding (12/1182 nt)	yjgN →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,477,321	0	A	.	99.7%	1354.6 / ‑3.2	293	coding (12/1182 nt)	yjgN	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (0/0); major base . (175/116); minor base C (0/1); total (175/118)
Fisher's exact test for biased strand distribution p-value = 4.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 293 total aligned reads displayed.

AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG  >  NC_000913/4477232‑4477408

aaCATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTcc                                                                                    <  1:6488954/93‑1 (MQ=255)

aaCATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTcc                                                                                    >  1:1635029/1‑93 (MQ=255)

  cATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGt                                                                                  >  1:7992343/1‑93 (MQ=255)

  cATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGt                                                                                  >  1:2533241/1‑93 (MQ=255)

   aTTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTc                                                                                 <  2:8021287/93‑1 (MQ=255)

    ttttACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTcc                                                                                >  2:7100111/1‑93 (MQ=255)

     tttAATGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTccc                                                                               <  2:2420732/93‑1 (MQ=255)

      ttACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCa                                                                              <  1:3090817/93‑1 (MQ=255)

         ctgtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTc                                                                           >  1:7290990/1‑93 (MQ=255)

          tgtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCAttag                                                                          >  1:3408685/1‑91 (MQ=255)

          tgtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCg                                                                          <  2:5694038/93‑1 (MQ=255)

           gtctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgt                                                                         >  2:925831/1‑93 (MQ=255)

             ctgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgttt                                                                       >  1:4520505/1‑93 (MQ=255)

              tgtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttg                                                                      >  2:3474850/1‑93 (MQ=255)

               gtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgt                                                                     >  2:5098093/1‑93 (MQ=255)

               gtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgt                                                                     >  2:7400038/1‑93 (MQ=255)

               gtATATATAAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCgtttgt                                                                     >  1:1069976/1‑93 (MQ=255)

                tatatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGACATGGAT‑GTTCCGTCCCATTCgtttgtt                                                                    >  1:3860135/1‑93 (MQ=255)

                  tatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGtttt                                                                  <  2:5551592/93‑1 (MQ=255)

                  tatataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGtttt                                                                  >  2:8514062/1‑93 (MQ=255)

                     ataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCa                                                               <  1:747800/93‑1 (MQ=255)

                     ataAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCa                                                               >  1:3665653/1‑93 (MQ=255)

                      taAGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCAt                                                              <  1:5000332/93‑1 (MQ=255)

                        aGTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATgg                                                            <  2:6622258/93‑1 (MQ=255)

                         gTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGt                                                           >  2:4601044/1‑93 (MQ=255)

                         gTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGt                                                           >  2:1137081/1‑93 (MQ=255)

                         gTTATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGt                                                           >  2:5961180/1‑93 (MQ=255)

                          ttATATCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTa                                                          <  2:890693/93‑1 (MQ=255)

                            atatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACa                                                        <  2:5055648/93‑1 (MQ=255)

                             tatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAg                                                       <  1:614467/93‑1 (MQ=255)

                             tatCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAg                                                       >  1:8097426/1‑93 (MQ=255)

                               tCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGt                                                     <  2:1454529/93‑1 (MQ=255)

                               tCAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGt                                                     >  2:2103443/1‑93 (MQ=255)

                                cAATGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTg                                                    >  2:324431/1‑93 (MQ=255)

                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGGAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   >  1:183370/1‑93 (MQ=255)

                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTGCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   <  2:7852674/93‑1 (MQ=255)

                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   <  2:3925034/93‑1 (MQ=255)

                                 aaTGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTga                                                   <  2:5370017/93‑1 (MQ=255)

                                   tGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgaga                                                 <  2:5731203/93‑1 (MQ=255)

                                   tGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgaga                                                 <  1:5648422/93‑1 (MQ=255)

                                   tGGATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgaga                                                 >  1:481227/1‑93 (MQ=255)

                                     gATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgagaga                                               >  2:4194885/1‑93 (MQ=255)

                                     gATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgagaga                                               <  1:3671976/93‑1 (MQ=255)

                                     gATTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTgagaga                                               >  2:1245603/1‑93 (MQ=255)

                                      aTTTTTTAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGat                                              <  2:8444991/93‑1 (MQ=255)

                                         ttttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtt                                           >  2:2489328/1‑93 (MQ=255)

                                          tttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAttt                                          >  2:2840983/1‑93 (MQ=255)

                                          tttAA‑TTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAttt                                          <  2:3685576/93‑1 (MQ=255)

                                            ttaattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtttt                                         <  2:4442407/89‑1 (MQ=255)

                                            ttaattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtttt                                         >  2:7430165/5‑93 (MQ=255)

                                            ttaattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGA‑CGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATAtttt                                         <  2:7529369/89‑1 (MQ=255)

                                             taattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTc                                        >  1:5819136/4‑93 (MQ=255)

                                              aattCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCt                                       <  1:5531492/91‑1 (MQ=255)

                                               attCAGGGAATTTTTATGGCTCACGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCtt                                      <  2:4786318/92‑1 (MQ=255)

                                               attCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCtt                                      <  1:2180234/92‑1 (MQ=255)

                                               attCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCtt                                      >  1:1716151/2‑93 (MQ=255)

                                                ttCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCttt                                     >  1:5899261/1‑92 (MQ=255)

                                                ttCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTa                                     >  2:3004919/1‑93 (MQ=255)

                                                 tCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAt                                    >  1:8239454/1‑93 (MQ=255)

                                                   aGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAttt                                  >  1:5277882/1‑93 (MQ=255)

                                                   aGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTAttt                                  <  2:7358998/93‑1 (MQ=255)

                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 <  2:6400899/93‑1 (MQ=255)

                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 <  2:6486494/93‑1 (MQ=255)

                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 >  1:1153153/1‑93 (MQ=255)

                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtg                                 >  1:6060318/1‑93 (MQ=255)

                                                    gggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGGGAGAGATATTTTCTTATTtg                                 >  2:2207517/1‑93 (MQ=255)

                                                     ggAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgt                                >  1:8602861/1‑93 (MQ=255)

                                                      gAATTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGATTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtg                               >  2:8309431/1‑93 (MQ=255)

                                                        aTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgtg                             <  1:6820152/93‑1 (MQ=255)

                                                        aTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgtg                             <  1:5958509/93‑1 (MQ=255)

                                                        aTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTtgtgtg                             >  1:7575718/1‑93 (MQ=255)

                                                        aTTTTTATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGACTTTTTCTTATTtgtgtg                             >  1:7829594/1‑93 (MQ=255)

                                                         tttttATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTgtgg                            >  1:2724581/1‑93 (MQ=255)

                                                         tttttATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTgtgg                            >  1:8451687/1‑93 (MQ=255)

                                                             tATGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGaa                        >  2:7284881/1‑93 (MQ=255)

                                                              aTGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAt                       <  1:4126218/93‑1 (MQ=255)

                                                              aTGGCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAt                       >  2:6304911/1‑93 (MQ=255)

                                                                tgCTTAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgt                     <  1:2867005/92‑1 (MQ=255)

                                                                ggCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgt                     >  1:8746712/1‑93 (MQ=255)

                                                                ggCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgt                     <  1:5045353/93‑1 (MQ=255)

                                                                 gCTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAAtgtg                    <  2:4951510/93‑1 (MQ=255)

                                                                  cTCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATgtgt                   >  1:6596271/1‑93 (MQ=255)

                                                                   tCAAGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGtgtt                  >  2:7776461/1‑93 (MQ=255)

                                                                   tCAAGTTATTAATAAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGtgtt                  >  2:2631794/1‑93 (MQ=255)

                                                                     aaGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGtgttgt                <  1:3477764/93‑1 (MQ=255)

                                                                      aGTTATTAATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTgttgtt               <  1:1393078/93‑1 (MQ=255)

                                                                         tattaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAAc            <  2:8228224/93‑1 (MQ=255)

                                                                           ttaATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGa          >  2:1566694/1‑93 (MQ=255)

                                                                            taATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAt         >  1:4205822/1‑93 (MQ=255)

                                                                            taATGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAt         >  2:1824305/1‑93 (MQ=255)

                                                                             aaTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAtt        >  1:5184513/1‑93 (MQ=255)

                                                                             aaTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGAtt        <  2:6179776/93‑1 (MQ=255)

                                                                              aTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATta       >  2:4415859/1‑93 (MQ=255)

                                                                              aTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATta       >  2:2965283/1‑93 (MQ=255)

                                                                              aTGAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATta       >  2:3834829/1‑93 (MQ=255)

                                                                                gAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATtata     <  2:7726461/93‑1 (MQ=255)

                                                                                gAAATGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATtata     <  2:1635029/93‑1 (MQ=255)

                                                                                  aaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAc   <  1:7090130/93‑1 (MQ=255)

                                                                                  aaTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAAc   <  2:4451478/93‑1 (MQ=255)

                                                                                   aTGGAT‑GTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACg  >  1:6391553/1‑93 (MQ=255)

AACATTTTACTGTCTGTATATATAAGTTATATCAATGGATTTTTTAATTTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATAGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACG  >  NC_000913/4477232‑4477408

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`