BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,265,000	(G)_5→6	coding (736/1467 nt)	yeiQ →	putative oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,264,995	1	.	G	99.7%	1018.1 / ‑4.1	294	coding (731/1467 nt)	yeiQ	putative oxidoreductase
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (129/164); total (129/165)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.77e-01

Only 100 of 337 total aligned reads displayed.

GATAAAAGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑GGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTG  >  NC_000913/2264903‑2265082

gATAAAAGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCt                                                                                          <  1:1568618/93‑1 (MQ=255)

gATAAAAGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCt                                                                                          >  2:5860768/1‑93 (MQ=255)

gATAAAAGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCt                                                                                          >  2:3203257/1‑93 (MQ=255)

gATAAAAGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCGGCAACCGACTGATCACTGGTGTGAATCAGCCACGATCt                                                                                          >  1:7691917/1‑93 (MQ=255)

 aTAAAAGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑g                                                                                        <  2:2106211/93‑1 (MQ=255)

      agagCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑gggggg                                                                                   >  1:6309723/1‑92 (MQ=255)

      agagCACGTCAGTTTTCCGGGAACAATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑gggggg                                                                                   <  2:6196814/93‑2 (MQ=255)

       gagCACGTCAGTTTTCCGGGAACCATGGTCGACAGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑ggggggg                                                                                  >  1:8647846/1‑91 (MQ=255)

           ccGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAAt                                                                               <  2:1063868/92‑1 (MQ=255)

           aCGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAAt                                                                               <  2:4627589/93‑1 (MQ=255)

           aCGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATATGGGGGGTGAAt                                                                               >  1:2469302/1‑93 (MQ=255)

            cGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGCCGAATCACTGGTGGAAACCAGCCAGCATCTGGGGGGTGAATg                                                                              >  1:6953626/1‑93 (MQ=255)

             gTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGa                                                                             <  1:6685418/93‑1 (MQ=255)

              tCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGAt                                                                            <  2:272495/93‑1 (MQ=255)

              tCAGTTTTCCGGGAACCATGGTCGACCGCATTGCTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGAt                                                                            >  2:4146098/1‑93 (MQ=255)

               cAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATc                                                                           <  1:4611890/93‑1 (MQ=255)

                aGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATcc                                                                          <  1:7133234/93‑1 (MQ=255)

                aGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATcc                                                                          >  2:7499134/1‑93 (MQ=255)

                 gTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCATCCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATccc                                                                         <  1:1216421/93‑1 (MQ=255)

                 gTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATccc                                                                         <  1:621090/93‑1 (MQ=255)

                 gTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATccc                                                                         <  1:4516823/93‑1 (MQ=255)

                 gTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATccc                                                                         <  1:3631786/93‑1 (MQ=255)

                    ttCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTgc                                                                      >  1:440581/1‑93 (MQ=255)

                    ttCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTgc                                                                      <  2:8388153/93‑1 (MQ=255)

                     tCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTgcg                                                                     >  1:8424150/1‑93 (MQ=255)

                      ccGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTgcgc                                                                    <  2:5104242/93‑1 (MQ=255)

                       cGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGcgcg                                                                   <  1:4308459/93‑1 (MQ=255)

                        gggAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGa                                                                  >  1:100341/1‑93 (MQ=255)

                        gggAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGa                                                                  <  1:6487926/93‑1 (MQ=255)

                        gggAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGa                                                                  <  2:1657899/93‑1 (MQ=255)

                          gAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGAtt                                                                >  1:8795623/1‑93 (MQ=255)

                          gAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGGGAATGATCCCTGCGCGAtt                                                                >  1:8005922/1‑93 (MQ=255)

                           aaCCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGAttg                                                               <  1:2630281/93‑2 (MQ=255)

                           aaCCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTa                                                               >  2:6624473/1‑93 (MQ=255)

                           aaCCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTa                                                               >  1:720234/1‑93 (MQ=255)

                           aaCCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATACCTGCGCGATTa                                                               >  1:1467964/1‑93 (MQ=255)

                            aCCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAg                                                              >  2:3377016/1‑93 (MQ=255)

                             ccATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGc                                                             <  1:827754/93‑1 (MQ=255)

                             ccATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGc                                                             <  2:2763439/93‑1 (MQ=255)

                             ccATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGc                                                             <  1:6093065/93‑1 (MQ=255)

                              cATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCt                                                            <  1:3377016/93‑1 (MQ=255)

                              cATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCt                                                            <  2:1804720/93‑1 (MQ=255)

                                tGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGc                                                          >  2:557146/1‑93 (MQ=255)

                                   tCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGaa                                                       >  2:8191379/1‑93 (MQ=255)

                                    cgtccGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAAc                                                      <  2:6519066/90‑1 (MQ=255)

                                     gACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATTCCTGCGCGATTAGCTGCGAAcc                                                     >  2:5033415/1‑93 (MQ=255)

                                     gACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAAcc                                                     <  2:8110469/93‑1 (MQ=255)

                                      aCCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTGGCTGCGAACCg                                                    >  1:2032652/1‑93 (MQ=255)

                                        cGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGtt                                                  <  2:2058809/93‑1 (MQ=255)

                                         gCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGttt                                                 >  2:7581636/1‑93 (MQ=255)

                                         gCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGttt                                                 >  2:6489049/1‑93 (MQ=255)

                                            ttGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATc                                              <  2:5507715/93‑1 (MQ=255)

                                             tGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATcc                                             <  2:1401947/93‑1 (MQ=255)

                                             tGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATcc                                             <  2:247672/93‑1 (MQ=255)

                                              gTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCa                                            <  1:5585947/93‑1 (MQ=255)

                                                tCCGGCTGCACCCGGCGACTCACTGGTGGCCGTCGGCCAGCCTCTGGGGGGTGAGTGATCCCTGCGCGATTCGCTGCGACCCGTTTATCCAgt                                          <  1:7842498/93‑1 (MQ=255)

                                                 ccGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTGGCTGCGAACCGTTTATCCAgtg                                         <  1:1346010/93‑1 (MQ=255)

                                                 ccGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtg                                         >  2:8756459/1‑93 (MQ=255)

                                                   ggCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtggg                                       <  2:4553610/93‑1 (MQ=255)

                                                   ggCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtggg                                       <  2:773876/93‑1 (MQ=255)

                                                    gCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgggg                                      >  2:6950834/1‑92 (MQ=255)

                                                     cTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAgtgggtg                                     <  2:4342049/93‑1 (MQ=255)

                                                       gCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGGGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGggtggt                                   >  1:545905/1‑93 (MQ=255)

                                                       gCAACCGACGAATAACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGggtggt                                   <  1:388250/93‑1 (MQ=255)

                                                        cAACCGACGACTCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGgtggtg                                  <  2:2963694/93‑1 (MQ=255)

                                                        cAACCGACCAACCGCTGGTGCAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTGGCTGCGAACCGTTTATCCAGTGGgtggtg                                  <  2:3592984/93‑1 (MQ=255)

                                                         aaCCGACGAATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGtggtgg                                 >  1:5060390/1‑93 (MQ=255)

                                                            cgacgaATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAg                              >  2:3960764/1‑93 (MQ=255)

                                                               cgaATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGTTGGAAGATa                           <  2:2796312/93‑1 (MQ=255)

                                                               cgaATCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATa                           >  2:5253180/1‑93 (MQ=255)

                                                                  aTCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACt                        >  2:536099/1‑93 (MQ=255)

                                                                  aTCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACt                        >  2:7779214/1‑93 (MQ=255)

                                                                  aTCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACt                        <  1:1271436/93‑1 (MQ=255)

                                                                   tCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACtt                       <  2:5762476/93‑1 (MQ=255)

                                                                   tCACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACtt                       <  2:2082900/93‑1 (MQ=255)

                                                                    cACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtc                      >  2:7185706/1‑93 (MQ=255)

                                                                    cACTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtc                      <  2:3828738/93‑1 (MQ=255)

                                                                     aCTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATACTTtcg                     >  1:2230813/1‑93 (MQ=255)

                                                                     aCTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcg                     >  1:5950477/1‑93 (MQ=255)

                                                                      cTGGTGGAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgt                    <  1:540468/93‑1 (MQ=255)

                                                                       tggtggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtc                   <  2:7357778/93‑1 (MQ=255)

                                                                        ggtggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTtcgtcg                  <  1:2856611/93‑1 (MQ=255)

                                                                         gtggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGc                 <  1:7038719/93‑1 (MQ=255)

                                                                         gtggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGc                 <  1:4172272/93‑1 (MQ=255)

                                                                           ggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTg               <  1:3205310/93‑1 (MQ=255)

                                                                           ggAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTg               <  2:4866734/93‑1 (MQ=255)

                                                                            gAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTgg              <  1:4018321/93‑1 (MQ=255)

                                                                            gAAATCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTgg              <  2:8594652/93‑1 (MQ=255)

                                                                             aaaTCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTggg             <  1:4962405/93‑1 (MQ=255)

                                                                              aaTCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGc            <  2:3734090/93‑1 (MQ=255)

                                                                               aTCAGCCAGCATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTTGAAGATAACTTCGTCGCTGGGCg           >  2:4835826/1‑93 (MQ=255)

                                                                                tCAGCCAGCATCTGGGGGGGGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGt          >  1:7318620/1‑93 (MQ=255)

                                                                                 cagccagcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTc         <  2:7906665/93‑1 (MQ=255)

                                                                                   gccagcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcct       >  1:5518622/1‑93 (MQ=255)

                                                                                   gccagcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcct       >  2:4493831/1‑93 (MQ=255)

                                                                                    ccagcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctg      <  1:3756597/93‑1 (MQ=255)

                                                                                      agcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcc    <  2:6138894/93‑1 (MQ=255)

                                                                                       gcATCTGGGGGGTGAATGATCCCTGCGCGATTGGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcct   <  1:8077304/93‑1 (MQ=255)

                                                                                       gcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcct   >  2:2415001/1‑93 (MQ=255)

                                                                                       gcATCTGGGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTcctgcct   <  1:1793844/93‑1 (MQ=255)

GATAAAAGAGCACGTCAGTTTTCCGGGAACCATGGTCGACCGCATTGTTCCGGCTGCAACCGACGAATCACTGGTGGAAATCAGCCAGCATCT‑GGGGGTGAATGATCCCTGCGCGATTAGCTGCGAACCGTTTATCCAGTGGGTGGTGGAAGATAACTTCGTCGCTGGGCGTCCTGCCTG  >  NC_000913/2264903‑2265082

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`