BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,781,020	+G	intergenic (‑144/+65)	ypjA ← / ← b2648	putative ATP‑binding component of a transport system/hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,781,019	1	.	G	100.0%	982.1 / NA	305	intergenic (‑143/+66)	ypjA/b2648	putative ATP‑binding component of a transport system/hypothetical protein
Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (148/157); total (148/157)

Only 100 of 327 total aligned reads displayed.

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG  >  NC_000913/2780927‑2781106

ggCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGa                                                                                          >  1:7148136/1‑93 (MQ=255)

ggCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGa                                                                                          <  1:6251616/93‑1 (MQ=255)

ggCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGa                                                                                          >  1:5635590/1‑93 (MQ=255)

 gCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑g                                                                                        >  2:477604/1‑93 (MQ=255)

 gCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑g                                                                                        >  2:6054156/1‑93 (MQ=255)

   aTTTTTTAAACGCAGTGCGCTTAAGTGTGGTTGGAGAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑ggc                                                                                      >  1:1530332/1‑91 (MQ=255)

   aTTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑ggc                                                                                      >  1:4007251/1‑91 (MQ=255)

   aTTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑ggc                                                                                      <  1:7829529/93‑3 (MQ=255)

         tAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTgg                                                                                 >  1:4658490/1‑92 (MQ=255)

         tAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTgg                                                                                 >  1:5986248/1‑92 (MQ=255)

          aaaCGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTggc                                                                                <  1:5671343/93‑3 (MQ=255)

          aaaCGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTggc                                                                                <  2:1581312/93‑3 (MQ=255)

           aaCGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCa                                                                               >  1:2072605/1‑93 (MQ=255)

            aCGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaa                                                                              >  1:5540265/1‑93 (MQ=255)

            aCGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaa                                                                              >  2:6752806/1‑93 (MQ=255)

            aCGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaa                                                                              <  2:1982543/93‑1 (MQ=255)

            aCGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaa                                                                              >  2:2245687/1‑93 (MQ=255)

              gCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                                            >  1:4832978/1‑93 (MQ=255)

              gCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                                            >  1:2898694/1‑93 (MQ=255)

              gCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaa                                                                            >  2:4669261/1‑93 (MQ=255)

               cAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGTAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaaa                                                                           >  2:7809237/1‑92 (MQ=21)

                aGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaata                                                                          >  1:5804985/1‑93 (MQ=255)

                 gTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataa                                                                         >  2:2689126/1‑93 (MQ=255)

                 gTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataa                                                                         <  1:4536393/93‑1 (MQ=25)

                  tGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaa                                                                        >  1:6714070/1‑93 (MQ=255)

                   gcgcAGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCaaaataaaa                                                                       <  2:1742982/93‑1 (MQ=25)

                      cTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGa                                                                    <  2:3306567/93‑1 (MQ=255)

                      cTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGa                                                                    <  2:5369882/93‑1 (MQ=25)

                       tGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAt                                                                   >  2:6456525/1‑93 (MQ=255)

                       tGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAt                                                                   >  2:1314703/1‑93 (MQ=255)

                        gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAtt                                                                  >  1:4073398/1‑93 (MQ=255)

                        gAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAtt                                                                  >  2:7685120/1‑93 (MQ=255)

                        gAAGTGTGGTTGGATAAAAAACTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAtt                                                                  <  1:2449195/93‑1 (MQ=25)

                         aaGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAttt                                                                 <  2:4266801/93‑1 (MQ=37)

                         aaGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGAttt                                                                 >  2:2383394/1‑93 (MQ=255)

                           gtgtGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTAc                                                               >  1:2510243/1‑93 (MQ=255)

                            tgtgGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCATTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACa                                                              >  2:5499763/1‑93 (MQ=37)

                               ggTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATaa                                                           >  1:6468653/1‑93 (MQ=255)

                                 ttGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                                         <  2:3829160/93‑1 (MQ=37)

                                 gtGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATc                                                         <  1:8105788/92‑1 (MQ=37)

                                      tAAAAAAGTCAATCCATTCAGGAAATGCGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTc                                                    <  1:303677/93‑1 (MQ=37)

                                      tAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTc                                                    >  2:745463/1‑93 (MQ=255)

                                       aaaaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCt                                                   <  2:8594382/93‑1 (MQ=37)

                                        aaaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtg                                                  <  1:5248505/93‑1 (MQ=37)

                                         aaaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtga                                                 >  1:8634020/1‑93 (MQ=255)

                                         aaaaGTCAATCCATTCAGGAAATACGGGCGTATGCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtga                                                 <  1:8489922/93‑1 (MQ=25)

                                          aaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCtgat                                                <  1:357527/93‑1 (MQ=38)

                                          aaaGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTAAATAATCGTTTCtgat                                                <  1:2115857/93‑1 (MQ=37)

                                              tCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAat                                            <  1:1025827/93‑1 (MQ=37)

                                               cAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAata                                           >  1:5855001/1‑93 (MQ=255)

                                                 aTCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtc                                         >  1:117575/1‑93 (MQ=255)

                                                  tCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtct                                        <  1:6604241/93‑1 (MQ=25)

                                                  tCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtct                                        <  1:6651462/93‑1 (MQ=25)

                                                  tCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGCTGGCAAAATAAACTGATTTACATAATCGTTTCTGCTGAATAtct                                        >  1:4327107/1‑93 (MQ=25)

                                                   ccATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtctt                                       <  2:3384387/93‑1 (MQ=25)

                                                   ccATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtctt                                       >  2:4496624/1‑93 (MQ=255)

                                                    cATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttc                                      >  1:7042394/1‑93 (MQ=255)

                                                     aTTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttct                                     >  1:891246/1‑93 (MQ=255)

                                                     aTTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttct                                     <  2:5923901/93‑1 (MQ=255)

                                                     aTTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATAtcttct                                     >  1:6165464/1‑93 (MQ=255)

                                                          ggAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTca                                >  1:1896612/1‑93 (MQ=255)

                                                          ggAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTca                                >  2:7119454/1‑93 (MQ=255)

                                                           gAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTcac                               >  1:2234260/1‑93 (MQ=255)

                                                           gAAATACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTcac                               >  1:5504928/1‑93 (MQ=255)

                                                            aaaTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTcaca                              <  1:4404802/93‑1 (MQ=21)

                                                              aTACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAAACGTTTCTGATGAATATCTTCTGCTCACATa                            <  2:4362376/93‑1 (MQ=21)

                                                               tACGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaa                           >  1:3338817/1‑93 (MQ=18)

                                                                 cGGGCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATaaaa                         <  2:4858526/93‑1 (MQ=255)

                                                                   ggCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAAt                       >  2:2838191/1‑93 (MQ=255)

                                                                    gCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATc                      >  1:6979020/1‑93 (MQ=18)

                                                                    gCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATc                      >  2:5704906/1‑93 (MQ=18)

                                                                    gCGTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATc                      <  2:3133700/93‑1 (MQ=17)

                                                                      gTATTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcac                    <  1:507249/93‑1 (MQ=255)

                                                                        aTTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacac                  >  2:7277118/1‑93 (MQ=18)

                                                                        aTTCTTTTCTTTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacac                  >  2:7143924/1‑93 (MQ=255)

                                                                         ttcttttcttTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacaca                 >  1:366531/1‑93 (MQ=17)

                                                                         ttcttttcttTCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATcacaca                 >  2:6664703/1‑93 (MQ=17)

                                                                          tcttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACaa                <  2:3511438/93‑1 (MQ=255)

                                                                          tcttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACaa                >  2:4102231/1‑93 (MQ=14)

                                                                          tcttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACaa                >  2:3718267/1‑93 (MQ=17)

                                                                          tcttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGACTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAATAATCACACaa                >  2:1093054/1‑93 (MQ=14)

                                                                           cttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAAt               <  2:6638686/93‑1 (MQ=255)

                                                                            ttttctttCGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATa              <  1:1438074/93‑1 (MQ=255)

                                                                             tttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATaa             <  1:3237594/93‑1 (MQ=255)

                                                                             tttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATaa             <  1:6054156/93‑1 (MQ=255)

                                                                              ttctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAAc            <  2:6230890/93‑1 (MQ=17)

                                                                               tctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACt           <  1:7405191/93‑1 (MQ=255)

                                                                               tctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACt           <  1:7662746/93‑1 (MQ=255)

                                                                                ctttcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACtt          <  1:2637745/93‑1 (MQ=255)

                                                                                 tttcGACAATGAGGCCGTTGGCAAAATATAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACttt         >  1:3485830/1‑93 (MQ=14)

                                                                                   tcGACAATGAGGCCGTTTGCAAAATAAAATGATTTACATAATCCTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTga       <  1:2752186/93‑1 (MQ=17)

                                                                                   tcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTga       >  1:1945595/1‑93 (MQ=17)

                                                                                   tcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTga       <  2:2874639/93‑1 (MQ=255)

                                                                                   tcGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTga       <  2:12447/93‑1 (MQ=255)

                                                                                    cGACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgag      <  2:1375813/93‑1 (MQ=255)

                                                                                     gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgaga     <  1:8270998/93‑1 (MQ=255)

                                                                                     gACAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTgaga     >  1:4948530/1‑93 (MQ=18)

                                                                                      aCAATGAGGCCGTTGGCAAGATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGAt    <  1:8571769/93‑1 (MQ=17)

                                                                                      aCAATGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGAt    >  2:5112833/1‑93 (MQ=18)

                                                                                        aaTGAGGCCGTTGGCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATAACACAATAACTTTGAGATCg  >  2:1644536/1‑93 (MQ=18)

GGCATTTTTTAAACGCAGTGCGCTGAAGTGTGGTTGGATAAAAAAGTCAATCCATTCAGGAAATACGGGCGTATTCTTTTCTTTCGACAATGA‑GCCGTTGCCAAAATAAAATGATTTACATAATCGTTTCTGATGAATATCTTCTGCTCACATAAAAATCACACAATAACTTTGAGATCG  >  NC_000913/2780927‑2781106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 35 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`