BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,544,357	+G	coding (163/1314 nt)	gntT →	gluconate permease

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,544,356	1	.	G	99.6%	869.3 / ‑3.9	252	coding (162/1314 nt)	gntT	gluconate permease
Reads supporting (aligned to +/- strand): ref base . (0/1); new base G (142/109); total (142/110)
Fisher's exact test for biased strand distribution p-value = 4.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

Only 100 of 293 total aligned reads displayed.

GGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑GGACG‑T‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544264‑3544437

ggCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGTCTCCATCAAAGCCGGTGTc                                                                                         <  1:2712147/93‑1 (MQ=255)

ggCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc                                                                                         <  1:6225656/93‑1 (MQ=255)

ggCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc                                                                                         <  2:8199635/93‑1 (MQ=255)

ggCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc                                                                                         <  2:6864626/93‑1 (MQ=255)

ggCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc                                                                                         >  1:6980922/1‑93 (MQ=255)

ggCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc                                                                                         <  2:2323288/93‑1 (MQ=255)

 gCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑g                                                                                       <  1:1597963/93‑1 (MQ=255)

 gCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑g                                                                                       >  2:7666566/1‑93 (MQ=255)

 gCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑g                                                                                       >  2:4688664/1‑93 (MQ=255)

  cTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑gg                                                                                      <  1:5565116/93‑2 (MQ=255)

   ttCATCGCTTTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggc                                                                                     <  2:897943/93‑3 (MQ=255)

   ttCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggc                                                                                     >  2:5105008/1‑91 (MQ=255)

   ttCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggc                                                                                     <  1:6526500/93‑3 (MQ=255)

     cATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTc‑ggcgg                                                                                   >  2:1425912/1‑89 (MQ=255)

                   tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGcc                                                                   >  1:3024103/1‑93 (MQ=255)

                    cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCt                                                                  <  2:5669478/93‑1 (MQ=255)

                    cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCt                                                                  <  1:1122701/93‑1 (MQ=255)

                      tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCttc                                                                >  1:7484291/1‑92 (MQ=255)

                         cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGccc                                                             >  2:3652483/1‑93 (MQ=255)

                         cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGccc                                                             <  1:3692823/93‑1 (MQ=255)

                         cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGccc                                                             <  1:767834/93‑1 (MQ=255)

                          gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCt                                                            >  2:2577405/1‑93 (MQ=255)

                          gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCt                                                            <  2:1726529/93‑1 (MQ=255)

                          gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCt                                                            <  1:6935881/93‑1 (MQ=255)

                           cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTg                                                           >  2:6605860/1‑93 (MQ=255)

                            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGa                                                          >  1:4551418/1‑93 (MQ=255)

                            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGa                                                          >  1:8138858/1‑93 (MQ=255)

                            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGa                                                          <  1:7234334/93‑1 (MQ=255)

                            ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGa                                                          >  2:6360071/1‑93 (MQ=255)

                             tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGAt                                                         >  1:4130753/1‑93 (MQ=255)

                             tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATCGGCTCGATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGAt                                                         <  2:4515445/93‑1 (MQ=255)

                               gtGTTTGATTAATGCAAGTAATCCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCa                                                       <  2:3455428/92‑1 (MQ=255)

                                tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCAt                                                      <  2:3832721/93‑1 (MQ=255)

                                tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCAt                                                      >  2:5285763/1‑93 (MQ=255)

                                 gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATg                                                     >  2:1537791/1‑93 (MQ=255)

                                 gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATg                                                     <  1:7893702/93‑1 (MQ=255)

                                 gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATg                                                     >  2:7822130/1‑93 (MQ=255)

                                  ttGGATTAAAGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATgg                                                    >  2:4833635/1‑93 (MQ=255)

                                   tGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATggg                                                   >  1:1823379/1‑93 (MQ=255)

                                    ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGAAGCT‑CGGTAGCCTTGCCCTGATCATGGGt                                                  >  2:3078647/1‑93 (MQ=255)

                                     gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGtt                                                 <  2:6148814/93‑1 (MQ=255)

                                     gATTAATGCAAGGAATGACGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGtt                                                 <  2:4285376/93‑1 (MQ=255)

                                      aTTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGttt                                                >  1:487744/1‑93 (MQ=255)

                                       ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGtttt                                               >  1:5955140/1‑93 (MQ=255)

                                          aTGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGgc                                            <  2:7166390/93‑1 (MQ=255)

                                              aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        <  1:5412833/93‑1 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       >  1:2036273/1‑93 (MQ=255)

                                               aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       >  1:2484309/1‑93 (MQ=255)

                                                ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATg                                      >  2:7877852/1‑93 (MQ=255)

                                                ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATg                                      >  2:203204/1‑93 (MQ=255)

                                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     >  1:2199304/1‑93 (MQ=255)

                                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  2:855434/93‑1 (MQ=255)

                                                 gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  1:4034772/93‑1 (MQ=255)

                                                   aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   <  2:2902597/93‑1 (MQ=255)

                                                   aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   >  1:143147/1‑93 (MQ=255)

                                                    tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  >  1:7107533/1‑93 (MQ=255)

                                                    tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  >  2:4570979/1‑93 (MQ=255)

                                                     gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTggg                                 >  2:1821266/1‑93 (MQ=255)

                                                      ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑GGAG‑GGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                <  2:4374142/93‑1 (MQ=255)

                                                       cGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCa                               >  1:2802873/1‑93 (MQ=255)

                                                       cGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCa                               >  1:984194/1‑93 (MQ=255)

                                                        gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:6388695/1‑93 (MQ=255)

                                                        gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              <  1:3271868/93‑1 (MQ=255)

                                                           ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           >  1:3360730/1‑93 (MQ=255)

                                                             aTAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGc                         >  1:73862/1‑93 (MQ=255)

                                                              tAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        <  2:3361492/93‑1 (MQ=255)

                                                              tAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        >  2:8597563/1‑93 (MQ=255)

                                                               aaaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       <  1:1101122/93‑1 (MQ=255)

                                                                   ttATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAg                   >  1:3403690/1‑93 (MQ=255)

                                                                    tATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGa                  >  2:2626553/1‑93 (MQ=255)

                                                                    tATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGa                  <  2:6487048/93‑1 (MQ=255)

                                                                    tATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGa                  >  1:2894050/1‑93 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  2:3482245/93‑1 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  2:1633669/93‑1 (MQ=255)

                                                                     aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGATTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 >  1:4272585/1‑93 (MQ=255)

                                                                      ttGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                <  2:3209329/93‑1 (MQ=255)

                                                                      ttGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                >  1:2374083/1‑93 (MQ=255)

                                                                       tGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg               <  2:7928089/93‑1 (MQ=255)

                                                                        ggCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc              >  1:8575686/1‑93 (MQ=255)

                                                                        ggCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc              <  1:5272446/93‑1 (MQ=255)

                                                                         gCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             <  1:7546440/93‑1 (MQ=255)

                                                                         gCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             >  1:5748752/1‑93 (MQ=255)

                                                                          cTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCgg            <  1:6811598/93‑1 (MQ=255)

                                                                          cTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGG‑TTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  2:7562028/93‑1 (MQ=255)

                                                                           tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGTCAGACTGCGGt           >  1:1710568/1‑93 (MQ=255)

                                                                           tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCggg           >  1:5834091/1‑92 (MQ=255)

                                                                           tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCggg           >  1:8074932/1‑92 (MQ=255)

                                                                           tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  1:6313081/93‑1 (MQ=255)

                                                                           tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  2:741942/93‑1 (MQ=255)

                                                                           tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           >  2:3933853/1‑93 (MQ=255)

                                                                            ccATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg          <  1:3220803/93‑1 (MQ=255)

                                                                             cATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         >  2:1112254/1‑93 (MQ=255)

                                                                             cATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGATTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         >  1:3546777/1‑93 (MQ=255)

                                                                              aTCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc        <  2:4269981/93‑1 (MQ=255)

                                                                              aTCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc        >  2:1318304/1‑93 (MQ=255)

                                                                               tCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  1:842842/1‑93 (MQ=255)

                                                                                  aaGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    >  2:2068801/1‑93 (MQ=255)

                                                                                  aaGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    >  1:3616888/1‑93 (MQ=255)

                                                                                   aGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCACTGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca   >  1:4673075/1‑93 (MQ=255)

                                                                                   aGCCGGTGTCGG‑‑CGGGACG‑CCCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGGGcaca   >  2:6973365/1‑93 (MQ=255)

GGCTTCATCGCTCTCGTCCTCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑GGACG‑T‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544264‑3544437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`