Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,544,360 +G coding (166/1314 nt) gntT → gluconate permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,544,3581.G99.2% 855.4 / ‑1.5 252coding (164/1314 nt)gntTgluconate permease
Reads supporting (aligned to +/- strand):  ref base . (0/2);  new base G (141/109);  total (141/111)
Fisher's exact test for biased strand distribution p-value = 1.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 258 total aligned reads displayed.

TCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑GGACG‑T‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544283‑3544437
                                                                               |                                                                                 
tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATGAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGcc                                                                   >  1:6898828/1‑93 (MQ=255)
tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGcc                                                                   <  2:6367591/93‑1 (MQ=255)
tCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGcc                                                                   >  2:7874799/1‑93 (MQ=255)
 cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCt                                                                  <  1:1122701/93‑1 (MQ=255)
 cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCt                                                                  <  1:7033471/93‑1 (MQ=255)
 cGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCt                                                                  <  1:3530686/93‑1 (MQ=255)
   tGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCttc                                                                >  1:7484291/1‑92 (MQ=255)
     gcgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGcc                                                              <  1:339666/93‑1 (MQ=255)
      cgcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGccc                                                             >  2:3652483/1‑93 (MQ=255)
       gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCt                                                            <  2:2040710/93‑1 (MQ=255)
       gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCt                                                            <  2:1726529/93‑1 (MQ=255)
       gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCt                                                            >  1:4823519/1‑93 (MQ=255)
       gcTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCt                                                            >  2:2577405/1‑93 (MQ=255)
        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGTCGCT‑CGGTAGCCTTGCCCTg                                                           >  1:7948388/1‑93 (MQ=255)
        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTTGCCTTGCCCTg                                                           >  2:1008849/1‑93 (MQ=255)
        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTg                                                           <  1:3745561/93‑1 (MQ=255)
        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTg                                                           >  2:6605860/1‑93 (MQ=255)
        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTg                                                           >  2:7640849/1‑93 (MQ=255)
        cTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTg                                                           >  2:1547143/1‑93 (MQ=255)
         ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGa                                                          >  1:8138858/1‑93 (MQ=255)
         ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGa                                                          >  2:6360071/1‑93 (MQ=255)
         ttGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGa                                                          >  2:116275/1‑93 (MQ=255)
          tGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGAt                                                         >  1:4130753/1‑93 (MQ=255)
            gtGTTTGATTAATGCAAGTAATCCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCa                                                       <  2:3455428/92‑1 (MQ=255)
             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCAt                                                      <  2:1442732/93‑1 (MQ=255)
             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCAt                                                      <  1:627221/93‑1 (MQ=255)
             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCAt                                                      >  2:5285763/1‑93 (MQ=255)
             tGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCAt                                                      <  2:3832721/93‑1 (MQ=255)
              gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATg                                                     >  1:5901012/1‑93 (MQ=255)
              gTTGGATTAATGCAAGGAATGCCGCTGGATAAAGGTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGGCTTGCCCTGATCATg                                                     >  1:7202386/1‑93 (MQ=255)
               ttGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGGCTTGCCCTGATCATgg                                                    >  2:3340416/1‑93 (MQ=255)
               ttGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATgg                                                    <  2:3028291/93‑1 (MQ=255)
                tGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATggg                                                   <  1:424422/93‑1 (MQ=255)
                 ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGt                                                  <  2:4517626/93‑1 (MQ=255)
                 ggATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGt                                                  >  1:178205/1‑93 (MQ=255)
                  gATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGtt                                                 <  2:6148814/93‑1 (MQ=255)
                    ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGtttt                                               >  1:5955140/1‑93 (MQ=255)
                    ttAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCAATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGtttt                                               >  1:1479646/1‑93 (MQ=255)
                     tAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCTGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTg                                              <  2:7335540/93‑1 (MQ=255)
                        tGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGgcg                                           <  1:2889959/93‑1 (MQ=255)
                         gCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGgcgc                                          >  1:504824/1‑93 (MQ=255)
                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        <  1:5412833/93‑1 (MQ=255)
                           aaGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCaa                                        <  2:3138006/93‑1 (MQ=255)
                            aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       >  1:5637416/1‑93 (MQ=255)
                            aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       >  1:2484309/1‑93 (MQ=255)
                            aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       <  1:7792207/93‑1 (MQ=255)
                            aGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAAt                                       <  1:2004114/93‑1 (MQ=255)
                             ggAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATg                                      >  2:203204/1‑93 (MQ=255)
                              gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  2:855434/93‑1 (MQ=255)
                              gAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGc                                     <  1:4034772/93‑1 (MQ=255)
                                aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   >  1:143147/1‑93 (MQ=255)
                                aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   <  2:2902597/93‑1 (MQ=255)
                                aTGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTg                                   >  1:4488540/1‑93 (MQ=255)
                                 tGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTgg                                  >  2:4570979/1‑93 (MQ=255)
                                  gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTggg                                 >  2:2600241/1‑93 (MQ=255)
                                  gCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTggg                                 >  2:1821266/1‑93 (MQ=255)
                                   gcGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                <  1:6956016/92‑1 (MQ=255)
                                   ccGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑GGAG‑GGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGc                                <  2:4374142/93‑1 (MQ=255)
                                    cGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCa                               >  1:2802873/1‑93 (MQ=255)
                                     gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:6388695/1‑93 (MQ=255)
                                     gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:3270703/1‑93 (MQ=255)
                                     gCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCaa                              >  1:8722355/1‑93 (MQ=255)
                                        ggATAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAAt                           >  1:3360730/1‑93 (MQ=255)
                                           tAAAGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCt                        <  2:4276772/93‑1 (MQ=255)
                                            aaaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTg                       <  1:1101122/93‑1 (MQ=255)
                                             aaGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTgg                      >  1:2278749/1‑93 (MQ=255)
                                              aGTTATTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGc                     <  1:4574106/93‑1 (MQ=255)
                                                  aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  2:1633669/93‑1 (MQ=255)
                                                  aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  1:2161189/93‑1 (MQ=255)
                                                  aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  2:433762/93‑1 (MQ=255)
                                                  aTTGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGAc                 <  2:3482245/93‑1 (MQ=255)
                                                   ttGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACt                >  2:8806225/1‑93 (MQ=255)
                                                    tGGCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTg               >  2:599260/1‑93 (MQ=255)
                                                     ggCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGCCTGc              >  1:3607799/1‑93 (MQ=255)
                                                     ggCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGc              <  1:5272446/93‑1 (MQ=255)
                                                      gCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             >  1:5794696/1‑93 (MQ=255)
                                                      gCTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCg             <  1:7546440/93‑1 (MQ=255)
                                                       cTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCgg            <  2:4789485/93‑1 (MQ=255)
                                                       cTCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGG‑TTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  2:7562028/93‑1 (MQ=255)
                                                        tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGTCAGACTGCGGt           >  1:1710568/1‑93 (MQ=255)
                                                        tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCggg           >  1:8074932/1‑92 (MQ=255)
                                                        tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  1:7356873/93‑1 (MQ=255)
                                                        tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  1:6313081/93‑1 (MQ=255)
                                                        tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           <  2:741942/93‑1 (MQ=255)
                                                        tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           >  2:3933853/1‑93 (MQ=255)
                                                        tCCATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCCGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGt           >  2:7902803/1‑93 (MQ=255)
                                                         ccATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg          <  2:4843853/93‑1 (MQ=255)
                                                         ccATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTg          >  1:3197031/1‑93 (MQ=255)
                                                          cATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         <  1:675766/93‑1 (MQ=255)
                                                          cATCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTgg         <  2:2131455/93‑1 (MQ=255)
                                                           aTCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgc        >  2:1318304/1‑93 (MQ=255)
                                                            tCAAAGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGgcg       >  1:842842/1‑93 (MQ=255)
                                                               aaGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    >  2:6874302/1‑93 (MQ=255)
                                                               aaGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    <  1:8529503/93‑1 (MQ=255)
                                                               aaGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcac    >  1:2941073/1‑93 (MQ=255)
                                                                aGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCTCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca   >  1:2470940/1‑93 (MQ=255)
                                                                aGCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGcaca   <  1:3645364/93‑1 (MQ=255)
                                                                aGCCGGTGTCGG‑‑CGGGACG‑CCCGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGGGcaca   >  2:6973365/1‑93 (MQ=255)
                                                                 gCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  >  2:8099272/1‑93 (MQ=255)
                                                                 gCCGGTGTCGG‑‑CGGGACGCT‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACaa  >  2:8597018/1‑93 (MQ=255)
                                                                               |                                                                                 
TCGTGGCGCTTGCTGTTGGATTAATGCAAGGAATGCCGCTGGATAAAGTTATTGGCTCCATCAAAGCCGGTGTC‑G‑‑C‑GGACG‑T‑CGGTAGCCTTGCCCTGATCATGGGTTTTGGCGCAATGCTGGGCAAAATGCTGGCAGACTGCGGTGGCGCACAA  >  NC_000913/3544283‑3544437

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑