Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,872,450 +G coding (338/387 nt) yidW ← regulator protein for dgo operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,872,4501.G99.2% 1222.2 / 1.2 356coding (338/387 nt)yidWregulator protein for dgo operon
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base G (196/157);  total (197/159)
Fisher's exact test for biased strand distribution p-value = 5.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

Only 100 of 394 total aligned reads displayed.

TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑CCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC  >  NC_000913/3872358‑3872536
                                                                                              |                                                                                       
 cGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑tg                                                                                         >  2:3793811/1‑92 (MQ=255)
  gTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑tgc                                                                                        >  2:7982217/1‑91 (MQ=255)
  gTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑tgc                                                                                        <  2:8372968/93‑3 (MQ=255)
    tAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑gccc                                                                                     <  2:3391244/93‑2 (MQ=255)
     aaaCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑gccca                                                                                    >  1:6161923/1‑91 (MQ=255)
      aaCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑GCCC‑Ac                                                                                  >  2:2490733/1‑93 (MQ=255)
       aCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑gcccgac                                                                                  <  1:6870690/93‑5 (MQ=255)
        cGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACg                                                                                 <  1:7060968/93‑1 (MQ=255)
         gCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑AAgg                                                                                >  1:6499437/1‑93 (MQ=255)
           ttCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGc                                                                              >  2:2848888/1‑93 (MQ=255)
           ttCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGc                                                                              >  1:8286268/1‑93 (MQ=255)
            tCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCa                                                                             >  2:2903307/1‑93 (MQ=255)
            tCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGAGCTCTGC‑TGCCCA‑ACGGGCa                                                                             >  2:8056614/1‑93 (MQ=255)
                 gTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGc                                                                        >  1:6374156/1‑93 (MQ=255)
                 gTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGc                                                                        >  2:8430691/1‑93 (MQ=255)
                 gTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGc                                                                        <  1:2499356/93‑1 (MQ=255)
                 gTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGc                                                                        <  2:6150995/93‑1 (MQ=255)
                  tCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGcc                                                                       >  2:8489720/1‑93 (MQ=255)
                     tGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGt                                                                    <  2:5048605/93‑1 (MQ=255)
                        ttgttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTcc                                                                 >  1:1087739/1‑93 (MQ=255)
                         tgttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTcca                                                                <  1:5990898/93‑1 (MQ=255)
                              gCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGa                                                           >  1:7580231/1‑93 (MQ=255)
                               cAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAt                                                          <  1:4378862/93‑1 (MQ=255)
                               cAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAt                                                          <  2:7119166/93‑1 (MQ=255)
                                aaTCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAtt                                                         >  1:6569463/1‑93 (MQ=255)
                                 atcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAttt                                                        >  1:8639804/1‑93 (MQ=255)
                                 atcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAttt                                                        <  1:4546610/93‑1 (MQ=255)
                                 atcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAttt                                                        >  2:2016493/1‑93 (MQ=255)
                                 accatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAttt                                                        >  1:8424834/3‑93 (MQ=255)
                                      ctcGTTCAGCGCCGATTCACTCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑CCGGGCAATCGCCGGTTCCACCCGATTTCGCAc                                                   <  1:7837754/93‑1 (MQ=255)
                                      ctcGTTCAGCGCCGATTAAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCAc                                                   <  2:8803472/93‑1 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  1:3091302/1‑93 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  <  1:8810372/93‑1 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  2:1050293/1‑93 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  <  1:7750922/93‑1 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  2:1487362/1‑93 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  1:3687740/1‑93 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  2:4349448/1‑93 (MQ=255)
                                        cGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACtt                                                 <  1:6965913/93‑1 (MQ=255)
                                        cGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACtt                                                 <  1:5159303/93‑1 (MQ=255)
                                         gtacAGCGCCTAGTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTc                                                <  2:4602088/90‑1 (MQ=255)
                                         gTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTc                                                >  2:3637274/1‑93 (MQ=255)
                                         gTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTc                                                >  2:6174673/1‑93 (MQ=255)
                                          ttCAGCGCCGATTCAATCTGCGCCAGATCGCTGTAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGCTCCACCAGATTTCGCACTTCg                                               >  1:1788407/1‑93 (MQ=255)
                                          ttCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCg                                               >  1:5062773/1‑93 (MQ=255)
                                          ttCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCg                                               >  2:8116443/1‑93 (MQ=255)
                                           tCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGc                                              <  2:6691719/93‑1 (MQ=255)
                                           tCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGc                                              >  1:1296175/1‑93 (MQ=255)
                                             aGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTc                                            >  1:2677545/1‑93 (MQ=255)
                                               cgcCGATTCAATCTGCGCCAGATCGCTAGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCAt                                          <  1:3954110/93‑1 (MQ=255)
                                                gcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATg                                         <  2:4678368/93‑1 (MQ=255)
                                                  cGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGc                                       <  1:752838/93‑1 (MQ=255)
                                                   gATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCa                                      >  1:7862486/1‑93 (MQ=255)
                                                   gATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCa                                      >  1:5268295/1‑93 (MQ=255)
                                                   gATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCa                                      >  2:3069818/1‑93 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     >  2:5682012/1‑93 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     >  1:8706758/1‑93 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     <  2:3240993/93‑1 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     <  2:1271115/93‑1 (MQ=255)
                                                     ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACt                                    <  2:2405801/93‑1 (MQ=255)
                                                     ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACt                                    >  1:4856640/1‑93 (MQ=255)
                                                     ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACt                                    >  2:3868805/1‑93 (MQ=255)
                                                      tCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTg                                   <  1:6779664/93‑1 (MQ=255)
                                                      tCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCACTGCAG‑CCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCACTCATGGCACTg                                   <  2:7834932/93‑1 (MQ=255)
                                                       cAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGa                                  <  2:6530669/93‑1 (MQ=255)
                                                       cAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGa                                  <  1:4810601/93‑1 (MQ=255)
                                                         agcTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATa                                <  2:7679884/91‑1 (MQ=255)
                                                            tGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGc                             <  2:6929155/93‑1 (MQ=255)
                                                            tGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGc                             <  2:1621574/93‑1 (MQ=255)
                                                            cgcgcCAGCTCGCTGGAAGTCGCGCACTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGc                             <  1:192717/92‑1 (MQ=255)
                                                             gcgcCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGcc                            <  1:53212/93‑1 (MQ=255)
                                                                ccAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTg                         <  2:2739179/93‑1 (MQ=255)
                                                                ccAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTg                         >  2:592464/1‑93 (MQ=255)
                                                                  aGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTggg                       <  2:6322399/93‑1 (MQ=255)
                                                                  aGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTggg                       <  2:2166437/93‑1 (MQ=255)
                                                                  aGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTggg                       >  2:4981641/1‑93 (MQ=255)
                                                                    aTCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTc                     >  2:3984281/1‑93 (MQ=255)
                                                                    aTCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTc                     >  1:7304883/1‑93 (MQ=255)
                                                                      cGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGt                   >  1:1180485/1‑93 (MQ=255)
                                                                       gCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTa                  >  1:2931941/1‑93 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                <  2:1719201/93‑1 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                <  2:8730377/93‑1 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                >  1:3896733/1‑93 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                >  1:4085380/1‑93 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGC‑TGCACA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                <  2:5271049/93‑1 (MQ=255)
                                                                             attCGCACTCTCTGC‑T‑ACGATACGGGCAATCGCCGGTTCCACCAGATTTCGCTCTTCGCTCATGGCACTGATAAGCCGTGGGTAGTAGTCAtt            <  2:4906222/91‑1 (MQ=255)
                                                                             aGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtt            >  2:5763623/1‑93 (MQ=255)
                                                                             aGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtt            <  1:4629903/93‑1 (MQ=255)
                                                                               tCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtttt          <  2:1156392/93‑1 (MQ=255)
                                                                                 gcgcgcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTcc        >  1:1659352/1‑93 (MQ=255)
                                                                                   gcgcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAg      >  2:5562537/1‑93 (MQ=255)
                                                                                   gcgcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAg      >  2:7293208/1‑93 (MQ=255)
                                                                                    cgcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGc     >  1:5475503/1‑93 (MQ=255)
                                                                                    cgcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGc     <  2:19089/93‑1 (MQ=255)
                                                                                     gcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCa    >  2:7533176/1‑93 (MQ=255)
                                                                                     gcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCa    >  1:3315371/1‑93 (MQ=255)
                                                                                     cctctGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCa    <  1:8095393/92‑1 (MQ=255)
                                                                                      ctctGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAc   >  1:3498073/1‑93 (MQ=255)
                                                                                       tctGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAcc  >  1:4235184/1‑93 (MQ=255)
                                                                                       tctGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAcc  <  1:740093/93‑1 (MQ=255)
                                                                                              |                                                                                       
TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑CCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC  >  NC_000913/3872358‑3872536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑