BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,603,663	(G)_8→9	noncoding (72/88 nt)	leuV ←	tRNA‑Leu

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,603,655	1	.	G	91.4%	655.9 / 55.3	222	noncoding (80/88 nt)	leuV	tRNA‑Leu
Reads supporting (aligned to +/- strand): ref base . (11/8); new base G (134/69); total (145/77)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 272 total aligned reads displayed.

TTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC  >  NC_000913/4603563‑4603747

   ttGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑gg                                                                                            >  1:6246943/1‑93 (MQ=255)

    tGCTTGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggg                                                                                           <  2:4639086/93‑1 (MQ=255)

     gCTGGCATCATGCTGATTATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑gggg                                                                                          <  1:5530004/93‑1 (MQ=255)

      cttgCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggg                                                                                         <  2:1972041/90‑1 (MQ=255)

      cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggg                                                                                         <  1:2963404/93‑1 (MQ=255)

      cTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggg                                                                                         <  1:8391873/93‑1 (MQ=255)

      cTGGCATCATGCTGATGATAATGAATATGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggg                                                                                         <  2:3540544/93‑1 (MQ=255)

       tGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑gggggg                                                                                        <  2:1326184/93‑1 (MQ=255)

        gggatcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggggg                                                                                       <  2:2937547/90‑1 (MQ=255)

        ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggggg                                                                                       >  1:5931288/1‑93 (MQ=255)

        ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggggg                                                                                       >  2:5247595/1‑93 (MQ=255)

        ggCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggggg                                                                                       >  2:5126588/1‑93 (MQ=255)

           atcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggggggac                                                                                    >  2:8167804/1‑90 (MQ=255)

            tcatGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑g‑ggggggggact                                                                                   >  1:485373/1‑89 (MQ=255)

                gCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGaa                                                                               >  1:374799/1‑93 (MQ=255)

                 cTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAAc                                                                              >  1:4269116/1‑93 (MQ=255)

                  tgatgaTAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGTTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAAcc                                                                             >  2:4804535/1‑93 (MQ=255)

                   gatgatAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAAccc                                                                            >  2:3798365/1‑93 (MQ=255)

                        tAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACACCCACg                                                                       >  1:2140156/1‑93 (MQ=255)

                         aaTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACgc                                                                      >  2:6492089/1‑92 (MQ=255)

                          aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTc                                                                     <  1:7059903/93‑1 (MQ=255)

                          aTGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTc                                                                     >  1:5548005/1‑93 (MQ=255)

                             aaTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATCATTGGCGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCACACGTCCgc                                                                  >  1:4388981/1‑92 (MQ=38)

                             aaTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCga                                                                  >  1:5481648/1‑92 (MQ=255)

                              aTTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTa                                                                 >  2:5863620/1‑93 (MQ=255)

                               tttGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAg                                                               >  2:4211055/1‑93 (MQ=255)

                                ttGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAGTTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAg                                                               <  2:6169763/93‑1 (MQ=255)

                                ttGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATGGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAg                                                               <  2:8480613/93‑1 (MQ=255)

                                    acgaGGGATGAAAAAGAAAGAAAGAAACAAGAAAGGAAGTAAAGATAATTGGGGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGaca                                                           <  2:5491287/90‑1 (MQ=25)

                                     tCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGacac                                                          >  2:4600711/1‑93 (MQ=255)

                                     acAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACt                                                         <  1:8685340/92‑1 (MQ=255)

                                        gggATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTaa                                                       <  1:8615925/93‑1 (MQ=255)

                                          gATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAaca                                                     >  2:4327681/1‑93 (MQ=255)

                                           aTGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGG‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAaca                                                     >  1:3869346/1‑93 (MQ=25)

                                           aTGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCATGTCCGTAAGGACACTAacac                                                    >  1:1296333/1‑93 (MQ=255)

                                            tGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGACACTAACAcc                                                   <  1:7326806/93‑1 (MQ=255)

                                               aaaaTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGa                                                >  1:1478666/1‑93 (MQ=255)

                                                  ataaaGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGGC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGc                                             <  2:6051704/90‑1 (MQ=21)

                                                  aTCAAGGAAGAAACAAGAAAGGAAGTAAAGATAGTTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGc                                             >  1:2476045/1‑93 (MQ=255)

                                                   tCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                            >  2:2169204/1‑93 (MQ=255)

                                                   acAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                            <  1:5492529/92‑1 (MQ=255)

                                                   acAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCt                                            <  1:5656249/92‑1 (MQ=255)

                                                    cAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTa                                           >  1:2837141/1‑93 (MQ=255)

                                                     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                          >  2:2447795/1‑93 (MQ=255)

                                                     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                          >  2:7033165/1‑93 (MQ=255)

                                                     aaGGAAGAAACAAGAAAGGAAGTAAAGATAATGGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAg                                          <  1:8105410/93‑1 (MQ=21)

                                                      aGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                         >  1:2242807/1‑93 (MQ=255)

                                                      aGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgc                                         >  1:6385064/1‑93 (MQ=255)

                                                       ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcg                                        >  1:6723610/1‑93 (MQ=255)

                                                       ggAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCAACGCCGTAAGGAGACTAACACCTGAAGCTAccg                                        >  2:4863252/1‑93 (MQ=21)

                                                        gaagaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAgcgc                                       >  1:3314335/1‑93 (MQ=255)

                                                          agaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGGCATTGAACCCCCAGTTCCGTCAGGACCCTAAAACCTGAACCTATCGCGt                                     >  2:6130822/1‑93 (MQ=18)

                                                           gaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAGG‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGt                                     >  1:881437/1‑93 (MQ=18)

                                                           gaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                    <  2:2841577/93‑1 (MQ=255)

                                                           gaaACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGGC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCt                                   >  1:4711536/1‑93 (MQ=255)

                                                           gaaACAAAAAAGGAATGAAAAATAATTGGTGCTGG‑‑‑G‑GGGGGGGAC‑TTGGACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                    <  1:5180623/93‑1 (MQ=14)

                                                           aaaaaaaaaaaGAAAGAAAAGATAATGGGTGCGGG‑‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTc                                    <  2:2330749/92‑1 (MQ=14)

                                                            aaaCAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGGC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                  <  1:6849400/93‑1 (MQ=255)

                                                             aaCAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                  >  2:6385833/1‑93 (MQ=255)

                                                             aaCAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                  >  2:8085050/1‑93 (MQ=255)

                                                             aaCAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑GTGGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTa                                  >  2:4456123/1‑93 (MQ=255)

                                                              aCAAGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTAc                                 >  1:2707470/1‑93 (MQ=255)

                                                                aaGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                               >  1:2785448/1‑93 (MQ=255)

                                                                aaGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCa                               >  1:4963302/1‑93 (MQ=255)

                                                                 aGAAAGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCaa                              >  2:5109150/1‑93 (MQ=255)

                                                                  gAAAGGAAGTAAAGATAATTGGTGC‑GAGG‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAAAACCTGAAGCTAGCGCGTCTACCaa                              >  2:6825171/1‑93 (MQ=17)

                                                                  aaaagaaagTAAAAATATTGGGGGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAt                             <  1:1981126/92‑1 (MQ=11)

                                                                   aaaGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAAtt                            >  1:430889/1‑93 (MQ=255)

                                                                    aaGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGGC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                           >  1:5001341/1‑93 (MQ=255)

                                                                    aaGGAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                           >  1:7175548/1‑93 (MQ=255)

                                                                    aaGGAAGAAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTc                           <  1:120206/93‑1 (MQ=14)

                                                                     aggaggTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                          <  2:1499388/93‑1 (MQ=255)

                                                                     aGGAAGTAAAGATAAGTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTcc                          <  1:8227117/93‑1 (MQ=255)

                                                                      ggAAGTAAAGATAATTGGTGC‑GAGGGG‑GGGGGGGAC‑TTGAACCCCCACGTCCATAAGTACACGAACACCGGAAGCTGGCCGCTCCACCAATTc                           >  2:8623599/1‑93 (MQ=9)

                                                                       gAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                        >  1:4123921/1‑93 (MQ=255)

                                                                       gAAGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGc                        >  1:1703461/1‑93 (MQ=255)

                                                                        aaGTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGcc                       <  1:2456166/93‑1 (MQ=255)

                                                                          gTAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGATAGCTCGTCTACCAATTCCGCCAc                     >  2:3879919/1‑93 (MQ=14)

                                                                           tAAAGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCAcc                    >  1:2341688/1‑93 (MQ=255)

                                                                            aaaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                   <  2:7612258/93‑1 (MQ=255)

                                                                            aaaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                   >  1:1558775/1‑93 (MQ=255)

                                                                            aaaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                   >  1:7020760/1‑93 (MQ=255)

                                                                            aaaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                   <  1:6125072/93‑1 (MQ=255)

                                                                            aaaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                   >  1:5572839/1‑93 (MQ=255)

                                                                            aaaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCt                   >  2:4551158/1‑93 (MQ=255)

                                                                             aaGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCtt                  >  2:5628643/1‑93 (MQ=255)

                                                                              tgATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                 <  1:443373/92‑1 (MQ=255)

                                                                              aGATAATTGGTGC‑GAG‑‑G‑GGGGGGGGC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                 >  1:7608601/1‑93 (MQ=255)

                                                                              aGATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGACCCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTc                 <  2:8757187/93‑1 (MQ=255)

                                                                               gATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                >  2:1087468/1‑93 (MQ=255)

                                                                               gATAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCg                >  2:1365964/1‑93 (MQ=255)

                                                                                aTAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGc               >  1:7642291/1‑93 (MQ=255)

                                                                                 tAATTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGca              >  2:7880439/1‑93 (MQ=16)

                                                                                  aaTTGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCTCCACCTTCGcac             >  1:7808299/1‑93 (MQ=255)

                                                                                     tGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGt          >  1:2759667/1‑93 (MQ=255)

                                                                                     tGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGt          <  1:4604953/93‑1 (MQ=255)

                                                                                     tGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGt          <  2:5691059/93‑1 (MQ=255)

                                                                                     tGGTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGt          <  1:1082627/93‑1 (MQ=255)

                                                                                      ggTGC‑GAG‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTc         <  2:7402778/93‑1 (MQ=255)

                                                                                             a‑‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCATAc  >  2:4528960/1‑93 (MQ=255)

TTATTGCTGGCATCATGCTGATGATAATGAATTTGAATCAGGGATGAAAAATCAAGGAAGAAACAAGAAAGGAAGTAAAGATAATTGGTGC‑GA‑‑‑G‑GGGGGGGAC‑TTGAACCCCCACGTCCGTAAGGACACTAACACCTGAAGCTAGCGCGTCTACCAATTCCGCCACCTTCGCACAGTCATCTTAC  >  NC_000913/4603563‑4603747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 16 ≤` `ATCG/ATCG` `< 26 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`