Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,329,903 A→G S278G (AGT→GGT)  topA → DNA topoisomerase type I omega protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,329,9030AG99.6% 971.2 / ‑5.7 281S278G (AGT→GGT) topADNA topoisomerase type I omega protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (138/142);  total (139/142)
Fisher's exact test for biased strand distribution p-value = 4.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 294 total aligned reads displayed.

CCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCATGATGATG  >  NC_000913/1329813‑1329992
                                                                                          |                                                                                         
ccgGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGt                                                                                         >  2:4654717/1‑93 (MQ=255)
  ggTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTaa                                                                                       <  2:6442868/93‑1 (MQ=255)
  ggTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTaa                                                                                       >  1:4847731/1‑93 (MQ=255)
    tCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAAc                                                                                     >  2:2317604/1‑93 (MQ=255)
     caacaaAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAAcc                                                                                    >  1:8220757/1‑93 (MQ=255)
       acaaAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTg                                                                                  <  1:7019246/93‑1 (MQ=255)
       acaaAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTg                                                                                  <  1:3615957/93‑1 (MQ=255)
       acaaAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTg                                                                                  <  2:3333420/93‑1 (MQ=255)
         aaaGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGgc                                                                                >  2:3842223/1‑93 (MQ=255)
             aaCAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTc                                                                            >  1:3424543/1‑93 (MQ=255)
             aaCAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTc                                                                            >  1:3072277/1‑93 (MQ=255)
              aCAAACTCAGGCTGCGGTAAGTCTGCTTGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTcc                                                                           <  2:4981468/93‑1 (MQ=255)
              aCAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTAGCGCTcc                                                                           >  2:4162128/1‑93 (MQ=255)
               cAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCt                                                                          <  1:7346007/93‑1 (MQ=255)
               cAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCt                                                                          <  1:2943753/93‑1 (MQ=255)
               cAAACTCAGGCTGCGGTAAGTATGCTGGTAAAAGTGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTATACCTGGCGCTCCt                                                                          >  2:6129719/1‑93 (MQ=255)
                aaaCTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCtt                                                                         >  2:3933037/1‑93 (MQ=255)
                aaaCTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCtt                                                                         <  2:3747662/93‑1 (MQ=255)
                 aaCTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCttt                                                                        >  2:7586737/1‑93 (MQ=255)
                 aaCTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCGACAGCGTGCTGGAAAGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCttt                                                                        >  1:7307520/1‑93 (MQ=255)
                   cTCAGGCTGGGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTtta                                                                      <  1:1596586/93‑1 (MQ=255)
                    tCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTttat                                                                     >  1:2590797/1‑93 (MQ=255)
                      aGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTttatta                                                                   >  2:2856117/1‑93 (MQ=255)
                        gCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTAcc                                                                 <  2:1185423/93‑1 (MQ=255)
                         cTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACct                                                                >  1:8182529/1‑93 (MQ=255)
                          tGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACctc                                                               <  2:1971997/93‑1 (MQ=255)
                          tGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACctc                                                               <  1:8541977/93‑1 (MQ=255)
                          tGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACctc                                                               <  2:586540/93‑1 (MQ=255)
                           gCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACctct                                                              >  2:5737643/1‑93 (MQ=255)
                           gCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACctct                                                              <  2:7380402/93‑1 (MQ=255)
                             ggTAAGTCTGCTGGAAAAAGTGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTAc                                                            >  1:5966388/1‑93 (MQ=255)
                             ggTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTAc                                                            <  1:184506/93‑1 (MQ=255)
                              gTAAGTCTGCTGGAAAAAGTGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTatg                                                           >  2:3599040/1‑91 (MQ=255)
                              gTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACg                                                           >  2:5667339/1‑93 (MQ=255)
                              gTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACg                                                           <  1:1167580/93‑1 (MQ=255)
                                 aGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTg                                                        <  1:3690169/93‑1 (MQ=255)
                                   tCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGca                                                      <  1:6757383/93‑1 (MQ=255)
                                       ctgGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGcaacaa                                                  <  1:3285808/93‑1 (MQ=255)
                                       ctgGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTACTTTTATTACCTCTACGCTGcaacaa                                                  >  1:4093111/1‑93 (MQ=255)
                                         tgAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGc                                                <  1:7993796/92‑1 (MQ=255)
                                         ggAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGc                                                >  1:4220509/1‑93 (MQ=255)
                                         ggAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGc                                                <  2:8612794/93‑1 (MQ=255)
                                           aaaaaGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTg                                              <  1:910448/93‑1 (MQ=255)
                                           aaaaaGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTg                                              >  1:8139069/1‑93 (MQ=255)
                                            aaaaGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCACCAAGCTGc                                             >  1:7458533/1‑93 (MQ=255)
                                            aaaaGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGATCCTTTTATTACCTCTACGCTGCAACAAGCTGc                                             >  2:7180437/1‑93 (MQ=255)
                                             aaaGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGcc                                            <  2:2154308/93‑1 (MQ=255)
                                              aaGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCa                                           >  2:7675172/1‑93 (MQ=255)
                                                gcgcgcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGTTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGc                                         <  2:5343729/93‑1 (MQ=255)
                                                gcgcgcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGc                                         <  2:6236397/93‑1 (MQ=255)
                                                gcgcgcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGc                                         >  1:3799631/1‑93 (MQ=255)
                                                gcgcgcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGc                                         >  2:796956/1‑93 (MQ=255)
                                                  gcgcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCAc                                       >  1:5369454/1‑93 (MQ=255)
                                                  gcgcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCAc                                       >  1:8327285/1‑93 (MQ=255)
                                                    gcTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCAccc                                     >  1:5912667/1‑93 (MQ=255)
                                                      tACAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGt                                   >  2:41346/1‑93 (MQ=255)
                                                       tcAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTc                                  <  1:5766681/92‑1 (MQ=255)
                                                       aCAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTc                                  <  1:1443083/93‑1 (MQ=255)
                                                        cAGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCt                                 >  1:2653160/1‑93 (MQ=255)
                                                         aGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCtt                                <  1:6356326/93‑1 (MQ=255)
                                                         aGCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCtt                                <  1:2904738/93‑1 (MQ=255)
                                                          gCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTg                               >  2:8508788/1‑93 (MQ=255)
                                                          gCGTGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTg                               <  2:3273642/93‑1 (MQ=255)
                                                             tGCTGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGAt                            >  2:5604246/1‑93 (MQ=255)
                                                                tGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTg                         <  2:4819220/93‑1 (MQ=255)
                                                                tGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTg                         <  1:3982062/93‑1 (MQ=255)
                                                                tGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTg                         >  2:6924980/1‑93 (MQ=255)
                                                                tGGAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTg                         >  1:5524599/1‑93 (MQ=255)
                                                                tGGAACATGAAGACAAACCGACAACCGGTAAACCTGGCGCTACTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTg                         <  2:5942345/93‑1 (MQ=255)
                                                                 ggAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTgg                        >  1:6177994/1‑93 (MQ=255)
                                                                  gAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGc                       >  1:3767339/1‑93 (MQ=255)
                                                                  gAACGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGc                       >  1:1342407/1‑93 (MQ=255)
                                                                    aCGTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGt                     >  1:4704832/1‑93 (MQ=255)
                                                                      gTGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGa                   <  1:2342721/93‑1 (MQ=255)
                                                                      gTGAAGACAAACCGACAACCGGTAAACCTGGAGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGa                   >  1:2115458/1‑93 (MQ=255)
                                                                       tGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCACCAGGCTGCCAGCACCCGTCTTGGATTTGGCGTGaa                  >  1:4556592/1‑93 (MQ=255)
                                                                       tGAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGaa                  >  1:6644183/1‑93 (MQ=255)
                                                                        gAAGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGaaa                 >  2:1812624/1‑93 (MQ=255)
                                                                         aaGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGaaaa                <  2:2985418/93‑1 (MQ=255)
                                                                         aaGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGaaaa                <  2:2081085/93‑1 (MQ=255)
                                                                          aGACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGaaaaa               >  2:8369906/1‑93 (MQ=255)
                                                                           gACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGaaaaaa              >  1:2177503/1‑93 (MQ=255)
                                                                           gACAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGaaaaaa              <  1:246958/93‑1 (MQ=255)
                                                                            aCAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGaaaaaaa             <  2:1464094/93‑1 (MQ=255)
                                                                            aCAAACCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGaaaaaaa             <  1:4997797/93‑1 (MQ=255)
                                                                               aaCCGAGAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTGGGATTTGGCGTGAAAAAACCCa          <  1:6834282/93‑1 (MQ=255)
                                                                               aaCCGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCa          >  1:1704017/1‑93 (MQ=255)
                                                                                  cGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatga       >  1:8649006/1‑93 (MQ=255)
                                                                                  cGACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatga       <  1:1133287/93‑1 (MQ=255)
                                                                                   gACAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgat      <  1:780766/93‑1 (MQ=255)
                                                                                    aCAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatg     >  1:4425960/1‑93 (MQ=255)
                                                                                    aCAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatg     >  2:7008897/1‑93 (MQ=255)
                                                                                    aCAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatg     >  2:7904340/1‑93 (MQ=255)
                                                                                     cAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatga    <  2:4463825/93‑1 (MQ=255)
                                                                                     cAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatga    <  2:8151341/93‑1 (MQ=255)
                                                                                     cAACCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGCCGTGAAAAAGACCataatga    >  1:6318233/1‑93 (MQ=255)
                                                                                      aaCCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatgat   >  1:2719609/1‑93 (MQ=255)
                                                                                       aCCGGTAATCCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatgatg  <  2:3145027/93‑1 (MQ=255)
                                                                                       aCCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatgatg  >  2:2598327/1‑93 (MQ=255)
                                                                                       aCCGGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCatgatgatg  >  1:1486649/1‑93 (MQ=255)
                                                                                          |                                                                                         
CCGGTCAACAAAGAACAAACTCAGGCTGCGGTAAGTCTGCTGGAAAAAGCGCGCTACAGCGTGCTGGAACGTGAAGACAAACCGACAACCAGTAAACCTGGCGCTCCTTTTATTACCTCTACGCTGCAACAAGCTGCCAGCACCCGTCTTGGATTTGGCGTGAAAAAAACCATGATGATG  >  NC_000913/1329813‑1329992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑