BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,397,365	A→G	G62G (GGT→GGC)	fnr ←	Fnr

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,397,365	0	A	G	99.4%	1172.2 / ‑6.0	343	G62G (GGT→GGC)	fnr	Fnr
Reads supporting (aligned to +/- strand): ref base A (0/2); new base G (151/190); total (151/192)
Fisher's exact test for biased strand distribution p-value = 5.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 358 total aligned reads displayed.

GAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGT  >  NC_000913/1397275‑1397454

 aaaCCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCa                                                                                        >  2:6045684/1‑93 (MQ=255)

     cAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCt                                                                                    <  1:6380029/93‑1 (MQ=255)

      aGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCtt                                                                                   >  2:967799/1‑93 (MQ=255)

         gATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTaaa                                                                                <  1:8624285/93‑1 (MQ=255)

         gATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTaaa                                                                                <  2:5895134/93‑1 (MQ=255)

           tttGCTCGTCGCCTTGCTCAGTGCTGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACa                                                                              <  2:4758658/93‑1 (MQ=255)

             tGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGc                                                                            <  1:1958054/93‑1 (MQ=255)

              gCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCg                                                                           <  2:4481226/93‑1 (MQ=255)

               cTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCGTAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGt                                                                          >  2:4127982/1‑93 (MQ=255)

               cTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGCACCGGAGCGGATGGCCTCACGCGATTTCAGTTCATCGCCAGACTTACACAGCgc                                                                          >  2:4342981/1‑92 (MQ=255)

                tcgtcgCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTc                                                                         <  2:318559/93‑1 (MQ=255)

                tcgtcgCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTc                                                                         >  1:4982522/1‑93 (MQ=255)

                tcgtcgCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTc                                                                         <  1:2472490/93‑1 (MQ=255)

                 cgtcgCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCt                                                                        <  2:3929154/93‑1 (MQ=255)

                  gtcgCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTg                                                                       >  2:7265617/1‑93 (MQ=255)

                  gtcgCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTg                                                                       >  2:3853032/1‑93 (MQ=255)

                     gCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGcc                                                                    >  1:1015747/1‑93 (MQ=255)

                     gCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGcc                                                                    <  1:1240344/93‑1 (MQ=255)

                     gCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGcc                                                                    >  1:5822537/1‑93 (MQ=255)

                      ccTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGAGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCt                                                                   >  1:4695462/1‑93 (MQ=255)

                       cTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCtt                                                                  <  1:6922615/93‑1 (MQ=255)

                        ttGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCttt                                                                 >  1:1522791/1‑93 (MQ=255)

                        ttGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCttt                                                                 >  2:833829/1‑93 (MQ=255)

                         tGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTc                                                                <  1:5393435/93‑1 (MQ=255)

                         tGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTc                                                                <  2:5951309/93‑1 (MQ=255)

                          tcTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCt                                                               <  1:3506263/92‑1 (MQ=255)

                          gCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCt                                                               <  2:5571571/93‑1 (MQ=255)

                           cTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTg                                                              <  1:3039944/93‑1 (MQ=255)

                            tCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGa                                                             >  1:4033709/1‑93 (MQ=255)

                             cAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGaa                                                            <  1:4645027/93‑1 (MQ=255)

                             cAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGaa                                                            <  2:172825/93‑1 (MQ=255)

                             cAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGaa                                                            <  2:5230429/93‑1 (MQ=255)

                             cAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGaa                                                            >  1:6215475/1‑93 (MQ=255)

                               gTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATa                                                          <  2:668795/93‑1 (MQ=255)

                               gTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATa                                                          >  2:5156445/1‑93 (MQ=255)

                               gTGATGGCATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATa                                                          <  2:2814657/93‑1 (MQ=255)

                                   tGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGct                                                      >  2:1494782/1‑93 (MQ=255)

                                     gTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGcttc                                                    <  2:485260/93‑1 (MQ=255)

                                      tataACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGcttct                                                   >  2:6239984/1‑93 (MQ=255)

                                      tataACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGcttct                                                   <  1:4818495/93‑1 (MQ=255)

                                       ataACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGcttctt                                                  >  1:6999694/1‑93 (MQ=255)

                                        taACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCttcttc                                                 >  1:1047657/1‑93 (MQ=255)

                                        taACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCttcttc                                                 >  1:4164249/1‑93 (MQ=255)

                                        taACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCttcttc                                                 <  1:4313865/93‑1 (MQ=255)

                                        taACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCttcttc                                                 >  1:72931/1‑93 (MQ=255)

                                         aaCTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTcc                                                >  1:7649201/1‑93 (MQ=255)

                                           cTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGc                                              >  2:6583623/1‑93 (MQ=255)

                                            ttttAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCt                                             >  1:2848066/1‑93 (MQ=255)

                                            ttttAATCGTACCGGAGCGGATGGCATAAAGCGACTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCt                                             >  1:774672/1‑93 (MQ=255)

                                               tAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCATCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCaa                                          <  1:8784276/93‑1 (MQ=255)

                                                aaTCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAAt                                         <  1:5973603/93‑1 (MQ=255)

                                                 aTCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATg                                        >  2:2890768/1‑93 (MQ=255)

                                                  tCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGa                                       <  2:5790276/93‑1 (MQ=255)

                                                  tCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGa                                       <  2:5667781/93‑1 (MQ=255)

                                                  tCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGa                                       >  1:4433327/1‑93 (MQ=255)

                                                   cGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGat                                      >  1:6723439/1‑93 (MQ=255)

                                                   cGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGat                                      <  1:8529511/93‑1 (MQ=255)

                                                    gTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGata                                     >  2:2368650/1‑93 (MQ=255)

                                                     tACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGatat                                    >  2:1607842/1‑93 (MQ=255)

                                                     tACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGatat                                    <  2:7725155/93‑1 (MQ=255)

                                                      aCCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGAtatt                                   <  2:1351308/93‑1 (MQ=255)

                                                      aCCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGAtatt                                   >  1:8245668/1‑93 (MQ=255)

                                                      aCCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGAtatt                                   <  1:5289981/93‑1 (MQ=255)

                                                       ccGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGAtatta                                  >  2:7438983/1‑93 (MQ=255)

                                                       ccGGAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGAtatta                                  <  1:2316611/93‑1 (MQ=255)

                                                         ggAGCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATc                                <  2:5631415/93‑1 (MQ=255)

                                                            gCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAg                             <  2:3653207/93‑1 (MQ=255)

                                                            gCGGATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAg                             >  1:4370867/1‑93 (MQ=255)

                                                              ggATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCt                           >  2:7241379/1‑93 (MQ=255)

                                                              ggATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCt                           <  2:538384/93‑1 (MQ=255)

                                                               gagtgCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTg                          <  2:7101743/89‑1 (MQ=255)

                                                               gATGGCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTg                          >  2:252474/1‑93 (MQ=255)

                                                                  ggCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATc                       <  1:3589634/93‑1 (MQ=255)

                                                                   gCATAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCa                      >  2:2700819/1‑93 (MQ=255)

                                                                     aTAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCGAg                    >  1:7127536/1‑93 (MQ=255)

                                                                     aTAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAg                    <  2:1836332/93‑1 (MQ=255)

                                                                      tAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGc                   <  1:2621119/93‑1 (MQ=255)

                                                                      tAAAGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGc                   >  1:1619931/1‑93 (MQ=255)

                                                                       aaaGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCt                  <  2:4028974/93‑1 (MQ=255)

                                                                        aaGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTc                 >  1:7965250/1‑93 (MQ=255)

                                                                        aaGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTc                 >  2:8171073/1‑93 (MQ=255)

                                                                        aaGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTc                 >  1:5142402/1‑93 (MQ=255)

                                                                         aGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCa                <  2:3046471/93‑1 (MQ=255)

                                                                         aGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCa                <  1:4586642/93‑1 (MQ=255)

                                                                         aGCGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCa                <  1:3291378/93‑1 (MQ=255)

                                                                           cGATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATg              <  2:1043078/93‑1 (MQ=255)

                                                                            gATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGt             <  1:1257512/93‑1 (MQ=255)

                                                                            gATTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGt             >  1:520413/1‑93 (MQ=255)

                                                                             aTTTAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGtt            >  1:527283/1‑93 (MQ=255)

                                                                                tAAGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCGAGCTCATGTTCGt         >  1:5579108/1‑93 (MQ=255)

                                                                                  aGTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTg       <  2:2279115/93‑1 (MQ=255)

                                                                                   gTTCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGa      <  2:6436696/93‑1 (MQ=255)

                                                                                    ttCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAg     <  2:5097326/93‑1 (MQ=255)

                                                                                    ttCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAg     <  2:3465718/93‑1 (MQ=255)

                                                                                    ttCATCGCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAg     <  1:742990/93‑1 (MQ=255)

                                                                                     tcatcgCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAgt    <  2:2114039/93‑1 (MQ=255)

                                                                                      catcgCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAgtg   >  1:3198312/1‑93 (MQ=255)

                                                                                       atcgCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAgtgt  <  2:6902384/93‑1 (MQ=255)

                                                                                       atcgCCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAgtgt  >  1:6615569/1‑93 (MQ=255)

                                                                                       atcgCCAGCCTTAAACAGCGGCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGAGCAAGCTCATGTTCGTTGAgtgt  <  2:7662098/93‑1 (MQ=255)

GAAACCAGTGATTTGCTCGTCGCCTTGCTCAGTGATGGTATAACTTTTAATCGTACCGGAGCGGATGGCATAAAGCGATTTAAGTTCATCACCAGCCTTAAACAGCGTCTGGCCTTTCTGAATAGGCTTCTTCCGCTCAATGATATTATCAAGCTGATCAAGCTCATGTTCGTTGAGTGT  >  NC_000913/1397275‑1397454

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 21 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`