BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,457,617	T→C	P180P (CCT→CCC)	ydbU →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,457,617	0	T	C	99.6%	812.4 / ‑5.6	241	P180P (CCT→CCC)	ydbU	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (95/144); total (97/144)
Fisher's exact test for biased strand distribution p-value = 4.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

Only 100 of 259 total aligned reads displayed.

ATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCTGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCAGGT  >  NC_000913/1457525‑1457706

   aaGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCcgtg                                                                                        <  1:3555294/93‑1 (MQ=255)

       tggtggAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCt                                                                                    <  2:3344412/93‑1 (MQ=255)

       tggtggAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCt                                                                                    <  1:2534627/93‑1 (MQ=255)

         gtggAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGt                                                                                  >  1:289080/1‑93 (MQ=255)

          tggAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGGGTTGGGGTACGCAGCCCGTGCGCTGTc                                                                                 >  1:5102320/1‑93 (MQ=255)

           ggAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCa                                                                                >  2:3702784/1‑93 (MQ=255)

            gAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCAt                                                                               <  2:7890518/93‑1 (MQ=255)

            gAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCAt                                                                               >  2:7377681/1‑93 (MQ=255)

               gtggtCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCg                                                                            >  2:1749412/1‑93 (MQ=255)

                tggtCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGa                                                                           <  2:1103078/93‑1 (MQ=255)

                 ggtCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGAc                                                                          <  1:115031/93‑1 (MQ=255)

                 ggtCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGAc                                                                          <  2:500932/93‑1 (MQ=255)

                   tCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTc                                                                        >  2:3128310/1‑93 (MQ=255)

                   tCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTc                                                                        >  1:4891028/1‑93 (MQ=255)

                    cAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTcc                                                                       <  2:1992567/93‑1 (MQ=255)

                     aGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCt                                                                      >  2:3403232/1‑93 (MQ=255)

                     aGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCt                                                                      >  1:2142869/1‑93 (MQ=255)

                     aGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCt                                                                      <  2:2707542/93‑1 (MQ=255)

                      gTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCGCGTGCGCTGTCATTCGACTCCTg                                                                     >  1:4257272/1‑93 (MQ=255)

                      gTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTg                                                                     <  1:2207367/93‑1 (MQ=255)

                      gTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTg                                                                     <  1:8184042/93‑1 (MQ=255)

                       tGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTgg                                                                    <  1:5260793/93‑1 (MQ=255)

                         ggCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGAt                                                                  >  1:3088900/1‑93 (MQ=255)

                            tGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTa                                                               >  1:3475395/1‑93 (MQ=255)

                              gCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATc                                                             >  1:7085121/1‑93 (MQ=255)

                                gaCGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGt                                                           <  2:1783938/92‑1 (MQ=255)

                                aaCGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGt                                                           >  2:1212631/1‑93 (MQ=255)

                                 aCGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGtt                                                          >  2:4553223/1‑93 (MQ=255)

                                 aCGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGtt                                                          <  1:6860480/93‑1 (MQ=255)

                                    gcggcggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAAc                                                       <  2:809557/93‑1 (MQ=255)

                                      ggcggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCg                                                     <  2:7085058/93‑1 (MQ=255)

                                      ggcggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCg                                                     <  1:5707818/93‑1 (MQ=255)

                                       gcggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCgt                                                    <  2:258435/93‑1 (MQ=255)

                                        cggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCggg                                                   >  1:3879671/1‑91 (MQ=255)

                                         ggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCgtgt                                                  >  1:7807512/1‑93 (MQ=255)

                                         ggAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCgtgt                                                  >  1:8517906/1‑93 (MQ=255)

                                           aaGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTg                                                <  1:6263594/93‑1 (MQ=255)

                                           aaGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTg                                                <  1:7595292/93‑1 (MQ=255)

                                            aGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTCTCGTTAACCGTGTTgc                                               <  2:5792026/93‑1 (MQ=255)

                                            aGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTgc                                               >  2:3592718/1‑93 (MQ=255)

                                            aGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTgc                                               <  2:4592045/93‑1 (MQ=255)

                                                gttgAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTAACATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGt                                           <  2:7787680/93‑1 (MQ=255)

                                                 ttgAGTAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTCCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGAGTTGCGCGTc                                          <  2:1958888/93‑1 (MQ=255)

                                                 ttgAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTc                                          >  1:2863109/1‑93 (MQ=255)

                                                 ttgAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTc                                          <  2:6159847/93‑1 (MQ=255)

                                                 ttgAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTc                                          <  2:1078369/93‑1 (MQ=255)

                                                  tgAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTcc                                         >  1:956606/1‑93 (MQ=255)

                                                   gAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCt                                        >  2:1017431/1‑93 (MQ=255)

                                                    aGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCtt                                       <  2:34647/93‑1 (MQ=255)

                                                     gCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCtta                                      >  1:3863470/1‑93 (MQ=255)

                                                     gCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCtta                                      >  2:6701940/1‑93 (MQ=255)

                                                         ttGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTtattat                                  <  2:7059048/93‑1 (MQ=255)

                                                           gtgtgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTc                                <  1:3205883/93‑1 (MQ=255)

                                                           gtgtgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTc                                <  2:2272112/93‑1 (MQ=255)

                                                           gtgtgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTc                                <  1:3729396/93‑1 (MQ=255)

                                                            tgtgAGCTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTcc                               <  2:2228899/93‑1 (MQ=255)

                                                            tgtgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTcc                               <  2:8176691/93‑1 (MQ=255)

                                                            tgtgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTcc                               <  2:5901942/93‑1 (MQ=255)

                                                             gtgAACTAACGTTGTGTTGGGGGTAGCAGGCTGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCg                              >  1:1531906/1‑93 (MQ=255)

                                                              tgAACTAACGTTGAGTTGGGGTAAGCTGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGa                             <  1:1220163/93‑1 (MQ=255)

                                                              tgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGa                             <  1:6093976/93‑1 (MQ=255)

                                                              tgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGa                             <  1:5874846/93‑1 (MQ=255)

                                                              tgAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGa                             <  1:7225678/93‑1 (MQ=255)

                                                               gAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAg                            >  2:4973443/1‑93 (MQ=255)

                                                               gAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAg                            <  1:6587407/93‑1 (MQ=255)

                                                               gAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAg                            <  1:4456712/93‑1 (MQ=255)

                                                               gAACTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAg                            <  2:6631787/93‑1 (MQ=255)

                                                                 aCTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGc                          >  2:2351305/1‑93 (MQ=255)

                                                                 aCTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCCGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGc                          >  1:7868374/1‑93 (MQ=255)

                                                                  cTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGcc                         >  1:361399/1‑93 (MQ=255)

                                                                  cTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGcc                         >  1:1788299/1‑93 (MQ=255)

                                                                  cTAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGcc                         <  2:854495/93‑1 (MQ=255)

                                                                   tAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCt                        <  1:5541673/93‑1 (MQ=255)

                                                                   tAACGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCt                        >  1:1724221/1‑93 (MQ=255)

                                                                    aaCGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTg                       >  1:7695527/1‑93 (MQ=255)

                                                                    aaCGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTg                       >  2:1332433/1‑93 (MQ=255)

                                                                      cGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGc                     <  1:6745496/93‑1 (MQ=255)

                                                                      cGTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGc                     <  1:642334/93‑1 (MQ=255)

                                                                       gTTGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCg                    <  2:7432799/93‑1 (MQ=255)

                                                                        ttGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCgg                   <  2:864926/93‑1 (MQ=255)

                                                                         tGAGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCggg                  <  1:2748220/93‑1 (MQ=255)

                                                                           aGTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCa                <  2:1806809/93‑1 (MQ=255)

                                                                            gTTGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCAc               <  1:1591359/93‑1 (MQ=255)

                                                                              tGGGGTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTg             >  1:1926008/1‑93 (MQ=255)

                                                                                gggTGAGCAACCCGTGCGCTGACATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGa           <  1:7143886/93‑1 (MQ=255)

                                                                                 ggTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGaa          <  2:5358066/93‑1 (MQ=255)

                                                                                 ggTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGaa          <  2:7596672/93‑1 (MQ=255)

                                                                                  gTAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAag         <  2:8471402/93‑1 (MQ=255)

                                                                                   tAAGCAGCCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAaga        <  1:902655/93‑1 (MQ=255)

                                                                                     agcagcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGc      <  2:3800269/93‑1 (MQ=255)

                                                                                     agcagcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGc      >  2:4738265/1‑93 (MQ=255)

                                                                                     agcagcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGc      <  2:5134054/93‑1 (MQ=255)

                                                                                      gcagcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCa     <  1:7021799/93‑1 (MQ=255)

                                                                                      gcagcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCa     <  1:2424639/93‑1 (MQ=255)

                                                                                      gcagcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCa     >  2:6453282/1‑93 (MQ=255)

                                                                                         gcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGATAAGCAGGt  >  2:4446668/1‑93 (MQ=255)

                                                                                         gcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCAGGt  <  1:3574980/93‑1 (MQ=255)

                                                                                         gcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCAGGt  <  2:4219107/93‑1 (MQ=255)

                                                                                         gcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCAGGt  <  2:3166470/93‑1 (MQ=255)

                                                                                         gcCCGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCAGGt  <  1:8346034/93‑1 (MQ=255)

ATGAAGTTGGTGGAGGTGGTCAGTGGGCTGGCAACGGCGGCGGAAGTTGTTGAGCAGTTGTGTGAACTAACGTTGAGTTGGGGTAAGCAGCCTGTGCGCTGTCATTCGACTCCTGGATTTATCGTTAACCGTGTTGCGCGTCCTTATTATTCCGAGGCCTGGCGGGCACTGGAAGAGCAGGT  >  NC_000913/1457525‑1457706

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`