BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,755,127	T→C	V469A (GTC→GCC)	pykF →	pyruvate kinase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,755,127	0	T	C	100.0%	752.3 / NA	216	V469A (GTC→GCC)	pykF	pyruvate kinase I
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (87/129); total (87/129)

Only 100 of 223 total aligned reads displayed.

AGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAA  >  NC_000913/1755037‑1755216

agagCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGccc                                                                                         <  1:6633913/93‑1 (MQ=255)

agagCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGccc                                                                                         <  1:8738136/93‑1 (MQ=255)

 gagCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCt                                                                                        >  1:7851904/1‑93 (MQ=255)

 gagCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCt                                                                                        >  1:5046296/1‑93 (MQ=255)

    cGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTa                                                                                     <  2:6621346/93‑1 (MQ=255)

     ggTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTaa                                                                                    <  2:147155/93‑1 (MQ=255)

      gTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAat                                                                                   >  2:265828/1‑93 (MQ=255)

       tCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAata                                                                                  <  2:8030657/93‑1 (MQ=255)

       tCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAata                                                                                  >  2:1396078/1‑93 (MQ=255)

       tCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAata                                                                                  >  1:3966694/1‑93 (MQ=255)

       tCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAata                                                                                  <  2:3000549/93‑1 (MQ=255)

        cTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAatat                                                                                 <  1:2639606/93‑1 (MQ=255)

         tGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATAtt                                                                                >  1:459375/1‑93 (MQ=255)

          ggCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTg                                                                               >  2:5157939/1‑93 (MQ=255)

            gacacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCt                                                                             <  1:6867441/92‑1 (MQ=255)

            cacacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCt                                                                             <  1:7536833/93‑1 (MQ=255)

            cacacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCt                                                                             >  1:3850054/1‑93 (MQ=255)

            cacacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCt                                                                             <  2:549093/93‑1 (MQ=255)

             acacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCtt                                                                            >  1:1014542/1‑93 (MQ=255)

             acacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCtt                                                                            >  1:2270523/1‑93 (MQ=255)

              cacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCttt                                                                           >  2:7780758/1‑93 (MQ=255)

              cacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCttt                                                                           >  1:5383095/1‑93 (MQ=255)

              cacaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCttt                                                                           >  1:1303531/1‑93 (MQ=255)

               acaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCtttt                                                                          <  2:1438120/93‑1 (MQ=255)

               acaAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCtttt                                                                          >  1:7200634/1‑93 (MQ=255)

                 aaaGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTtgt                                                                        <  1:7951918/93‑1 (MQ=255)

                 aaaGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTtgt                                                                        >  1:5833253/1‑93 (MQ=255)

                   aGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGa                                                                      >  1:5254711/1‑93 (MQ=255)

                    ggTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACTGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaa                                                                     >  2:7352713/1‑93 (MQ=255)

                    ggTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaa                                                                     <  1:4714132/93‑1 (MQ=255)

                     gTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaat                                                                    <  2:8615207/93‑1 (MQ=255)

                      tGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaatt                                                                   >  1:4820088/1‑93 (MQ=255)

                        aCGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaattaa                                                                 >  1:6407223/1‑93 (MQ=255)

                         cGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaattaat                                                                >  1:2860288/1‑93 (MQ=255)

                          gTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaattaatt                                                               <  1:8049238/93‑1 (MQ=255)

                          gTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGaattaatt                                                               <  2:1938015/93‑1 (MQ=255)

                           ttGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAAttt                                                              <  1:1211795/93‑1 (MQ=255)

                            tGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTg                                                             <  2:715569/93‑1 (MQ=255)

                             gTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTGCTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGt                                                            <  2:2013119/93‑1 (MQ=255)

                              tAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGta                                                           <  1:2060094/93‑1 (MQ=255)

                              tAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGta                                                           >  1:353385/1‑93 (MQ=255)

                              tAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGta                                                           <  2:5457938/93‑1 (MQ=255)

                               aGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGtat                                                          <  2:7686933/93‑1 (MQ=255)

                                gTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGttta                                                         <  2:7814544/93‑1 (MQ=255)

                                gTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGtata                                                         <  1:8391293/93‑1 (MQ=255)

                                 ttATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTatat                                                        <  1:6347782/93‑1 (MQ=255)

                                 ttATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTatat                                                        <  2:3628280/93‑1 (MQ=255)

                                  tATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATc                                                       <  1:4294238/93‑1 (MQ=255)

                                  tATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATc                                                       <  2:1609967/93‑1 (MQ=255)

                                   aTGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCg                                                      <  2:5608525/93‑1 (MQ=255)

                                     ggTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGaa                                                    <  2:1211958/93‑1 (MQ=255)

                                     ggTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGaa                                                    <  2:2237872/93‑1 (MQ=255)

                                     ggTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGaa                                                    >  1:1678399/1‑93 (MQ=255)

                                      gTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAg                                                   <  2:2033585/93‑1 (MQ=255)

                                       tttCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAgc                                                  <  2:1096852/93‑1 (MQ=255)

                                       tttCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAgc                                                  >  2:6436082/1‑93 (MQ=255)

                                          cTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGcc                                               <  2:7952789/93‑1 (MQ=255)

                                           tGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGtcc                                              >  2:2070043/1‑93 (MQ=255)

                                           tGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGgcc                                              >  2:3091501/1‑93 (MQ=255)

                                            ggTGCACTGGTACCGAGCGGCACTACTAACGCCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCt                                             <  1:3138202/93‑1 (MQ=255)

                                            ggTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTTTGAATTAATTTGTATATCGAAGCGCCCt                                             <  2:7408807/93‑1 (MQ=255)

                                               gCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGAt                                          >  1:95824/1‑93 (MQ=255)

                                                cACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATg                                         <  2:1803961/93‑1 (MQ=255)

                                                cACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATg                                         <  2:2219076/93‑1 (MQ=255)

                                                 aCTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATgg                                        >  1:8652356/1‑93 (MQ=255)

                                                  cTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTGATTTGTATATCGAAGCGCCCTGATggg                                       <  1:1500610/93‑1 (MQ=255)

                                                     gTACCGAGCGGCACTACTAACACCGCATCTGTTCACTCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGACCTGGTGGgcgc                                    >  2:1830651/1‑93 (MQ=255)

                                                     gTACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGgcgc                                    <  2:2376380/93‑1 (MQ=255)

                                                      tACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCt                                   <  1:3503907/93‑1 (MQ=255)

                                                      tACCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCt                                   <  1:8474451/93‑1 (MQ=255)

                                                       aCCGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCtt                                  <  1:300619/93‑1 (MQ=255)

                                                        ccGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCttt                                 <  1:5792135/93‑1 (MQ=255)

                                                        ccGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCttt                                 <  1:7781156/93‑1 (MQ=255)

                                                         cGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCtttt                                <  1:7652005/93‑1 (MQ=255)

                                                         cGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCtttt                                >  2:4250703/1‑93 (MQ=255)

                                                         cGAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCtttt                                <  1:4413527/93‑1 (MQ=255)

                                                          gAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCttttt                               <  1:2481631/93‑1 (MQ=255)

                                                          gAGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCttttt                               <  2:6333485/93‑1 (MQ=255)

                                                           aGCGGCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCtttttt                              <  1:2667607/93‑1 (MQ=255)

                                                               gCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTtttatt                          >  1:5010584/1‑93 (MQ=255)

                                                               gCACTACTAACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTtttatt                          <  1:4145964/93‑1 (MQ=255)

                                                                   tactaACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAAt                      >  2:8386349/1‑93 (MQ=255)

                                                                    actaACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATc                     <  1:2189545/93‑1 (MQ=255)

                                                                      taACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGa                   >  2:6084574/1‑93 (MQ=255)

                                                                      taACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGa                   <  1:7268116/93‑1 (MQ=255)

                                                                      taACACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGa                   <  2:3334895/93‑1 (MQ=255)

                                                                       aaCACCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGAt                  <  2:7613636/93‑1 (MQ=255)

                                                                        acacCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATa                 >  1:7113537/1‑93 (MQ=255)

                                                                        acacCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGCGCGCTTTTTTTATTTAATCGATa                 >  2:4754348/1‑93 (MQ=255)

                                                                          acCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAAc               <  1:7332576/93‑1 (MQ=255)

                                                                          acCGCATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAAc               <  1:6964952/93‑1 (MQ=255)

                                                                            cGCATCTGTTCACTCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCa             >  2:7518177/1‑93 (MQ=255)

                                                                              cATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGGCCTGATGGGCGCTTTTTTTATTTAATCGATAAACAGa           >  2:4855142/1‑93 (MQ=255)

                                                                              cATCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGa           <  1:65618/93‑1 (MQ=255)

                                                                               aTCTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGaa          <  2:8211334/93‑1 (MQ=255)

                                                                                 cTGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGc        <  1:7903145/93‑1 (MQ=255)

                                                                                  tGTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCa       <  1:6573996/93‑1 (MQ=255)

                                                                                   gTTCACGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCaa      >  2:4005750/1‑93 (MQ=255)

                                                                                       aCGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATaaa  >  1:1936972/1‑93 (MQ=255)

                                                                                       aCGCCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATaaa  >  1:8351185/1‑93 (MQ=255)

AGAGCGGTCTGGCACACAAAGGTGACGTTGTAGTTATGGTTTCTGGTGCACTGGTACCGAGCGGCACTACTAACACCGCATCTGTTCACGTCCTGTAATATTGCTTTTGTGAATTAATTTGTATATCGAAGCGCCCTGATGGGCGCTTTTTTTATTTAATCGATAACCAGAAGCAATAAA  >  NC_000913/1755037‑1755216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 31 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`