BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,848,059	T→C	V400A (GTT→GCT)	sppA →	protease IV

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,848,059	0	T	C	99.2%	894.6 / ‑3.0	262	V400A (GTT→GCT)	sppA	protease IV
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (126/134); total (127/135)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.78e-01

Only 100 of 272 total aligned reads displayed.

TGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACC  >  NC_000913/1847969‑1848147

   gtgtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTAt                                                                                     <  2:3849069/93‑1 (MQ=255)

   gtgtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTAt                                                                                     <  1:7891685/93‑1 (MQ=255)

    tgtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATc                                                                                    >  1:1587597/1‑93 (MQ=255)

    tgtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATc                                                                                    <  1:7732358/93‑1 (MQ=255)

    tgtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATc                                                                                    >  1:3980768/1‑93 (MQ=255)

     gtTAATAGCCCAGGCGGCAGCGTTCCCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCg                                                                                   <  2:1854799/93‑1 (MQ=255)

     gtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCg                                                                                   <  2:2164423/93‑1 (MQ=255)

     gtTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCg                                                                                   >  1:814406/1‑93 (MQ=255)

      ttAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGa                                                                                  <  2:5682872/93‑1 (MQ=255)

       tAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGAt                                                                                 <  1:8362778/93‑1 (MQ=255)

        aaTAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATg                                                                                <  2:4252122/93‑1 (MQ=255)

        aaTAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATg                                                                                <  1:4933593/93‑1 (MQ=255)

         aTAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATgg                                                                               <  2:1486694/93‑1 (MQ=255)

         aTAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATgg                                                                               >  2:4523393/1‑93 (MQ=255)

         aTAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATgg                                                                               <  2:5949479/93‑1 (MQ=255)

           aGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggc                                                                             >  2:6111716/1‑93 (MQ=255)

           aGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggc                                                                             <  1:5627509/93‑1 (MQ=255)

            gCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcg                                                                            >  2:125736/1‑93 (MQ=255)

            gCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcg                                                                            >  2:2710094/1‑93 (MQ=255)

             cccAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcgg                                                                           <  2:6677358/93‑1 (MQ=255)

              ccAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATCggcggc                                                                          >  2:2806865/1‑93 (MQ=255)

               cAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcggca                                                                         <  2:3903783/93‑1 (MQ=255)

               cAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcggca                                                                         >  1:4220682/1‑93 (MQ=255)

               cAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcggca                                                                         <  2:3617466/93‑1 (MQ=255)

                aGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcggcat                                                                        <  1:5107765/93‑1 (MQ=255)

                aGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcggcat                                                                        >  1:4368878/1‑93 (MQ=255)

                aGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcggcat                                                                        >  1:694936/1‑93 (MQ=255)

                 ggcggcAGTGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcggcatg                                                                       <  1:268986/93‑1 (MQ=255)

                 ggcggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcggcatg                                                                       <  2:4196720/93‑1 (MQ=255)

                   cggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcggcatggc                                                                     <  1:7529524/93‑1 (MQ=255)

                   cggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcggcatggc                                                                     >  1:3662992/1‑93 (MQ=255)

                   cggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcggcatggc                                                                     <  1:7313827/93‑1 (MQ=255)

                    ggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcggcatggcg                                                                    >  2:1703871/1‑93 (MQ=255)

                    ggcAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGggcggcatggcg                                                                    >  2:640286/1‑93 (MQ=255)

                         cGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCCTc                                                               >  1:2097772/1‑93 (MQ=255)

                          gTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCt                                                              <  1:7592662/93‑1 (MQ=255)

                          gTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCt                                                              >  2:8120178/1‑93 (MQ=255)

                          gTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCt                                                              >  2:5406163/1‑93 (MQ=255)

                           ttACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCtg                                                             <  2:4933791/93‑1 (MQ=255)

                           ttACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCtg                                                             >  1:4447570/1‑93 (MQ=255)

                             aCCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCtggt                                                           >  1:5016550/1‑93 (MQ=255)

                             aCCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGAGGCATGGCGGCATCtggt                                                           <  1:7326593/93‑1 (MQ=255)

                                gcgTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTggtggt                                                        >  1:4194698/1‑93 (MQ=255)

                                gcgTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTggtggt                                                        <  1:8627053/93‑1 (MQ=255)

                                gcgTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTggtggt                                                        <  1:6869935/93‑1 (MQ=255)

                                 cgTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGtt                                                       <  1:945416/93‑1 (MQ=255)

                                  gTCTGTAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTa                                                      <  2:3027180/93‑1 (MQ=255)

                                   tCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTAc                                                     <  2:6892621/93‑1 (MQ=255)

                                     tGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTg                                                   <  2:8259104/93‑1 (MQ=255)

                                     tGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTg                                                   <  2:2492128/93‑1 (MQ=255)

                                     tGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTg                                                   >  2:5897898/1‑93 (MQ=255)

                                      gAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTgg                                                  >  2:7249688/1‑93 (MQ=255)

                                      gAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTgg                                                  >  1:8536423/1‑93 (MQ=255)

                                       aaGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGa                                                 <  1:5966370/93‑1 (MQ=255)

                                        aGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGAt                                                >  2:1929348/1‑93 (MQ=255)

                                           gATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTc                                             >  2:735534/1‑93 (MQ=255)

                                           gATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTc                                             >  2:741984/1‑93 (MQ=255)

                                           gATTCGCGCTGAACTCGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTc                                             <  2:2641344/93‑1 (MQ=255)

                                             ttCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCa                                           >  1:5681599/1‑93 (MQ=255)

                                              tCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCAc                                          <  2:8021706/93‑1 (MQ=255)

                                                 cgcTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGcc                                       <  2:6515941/93‑1 (MQ=255)

                                                  gcTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCa                                      >  2:8487874/1‑93 (MQ=255)

                                                  gcTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCa                                      >  2:2947500/1‑93 (MQ=255)

                                                   cTGAACTGGCAGCAGCCCGGGCAGCTGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAg                                     <  1:908785/93‑1 (MQ=255)

                                                   cTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAg                                     >  2:3349410/1‑93 (MQ=255)

                                                     gAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTCATGGATTTCAACGCCAGca                                   >  1:3942622/1‑92 (MQ=255)

                                                     gAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCt                                   >  1:1724810/1‑93 (MQ=255)

                                                        cTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAAt                                >  1:1159899/1‑93 (MQ=255)

                                                          ggCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCGCGCCAGCTAATTa                              <  2:32312/93‑1 (MQ=255)

                                                            cagcagCCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACa                            >  2:7942941/1‑93 (MQ=255)

                                                             agcagcCCGGGCAGCGGGTAAGCCTGTGGCTGTCTCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCCGATAATTCCAt                           <  2:7791817/93‑1 (MQ=255)

                                                              gcagcCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACAtt                          <  2:8702262/93‑1 (MQ=255)

                                                              gcagcCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACAtt                          <  2:2359934/93‑1 (MQ=255)

                                                              gcagcCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACAtt                          <  2:2321023/93‑1 (MQ=255)

                                                              gcagcCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACAtt                          <  1:1835522/93‑1 (MQ=255)

                                                              gcagcCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACAtt                          <  1:5772742/93‑1 (MQ=255)

                                                              gcagcCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACAtt                          >  1:7274879/1‑93 (MQ=255)

                                                                 gcCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATtgtg                       >  2:4441406/1‑93 (MQ=255)

                                                                 gcCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATtgtg                       >  2:2048731/1‑93 (MQ=255)

                                                                 gcCCGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATtgtg                       <  1:5249360/93‑1 (MQ=255)

                                                                   ccGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGc                     <  1:4581623/93‑1 (MQ=255)

                                                                   ccGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGGGGTTACTGGATTTCAACGCCAGCTAATTACATTGTGGc                     >  1:1129473/1‑93 (MQ=255)

                                                                    cGGGCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCt                    >  2:1140982/1‑93 (MQ=255)

                                                                     gggCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTa                   <  2:3569452/93‑1 (MQ=255)

                                                                      ggCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTaa                  >  2:8185526/1‑93 (MQ=255)

                                                                       gCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAAc                 >  1:7568165/1‑93 (MQ=255)

                                                                       gCAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAAc                 <  1:5352232/93‑1 (MQ=255)

                                                                        cAGCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAAcc                <  2:4677658/93‑1 (MQ=255)

                                                                          gCGGGTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGGTACTGGATTTCCACGCCAGCTCATTACATTGTGGCTAAcccc              >  1:6443641/1‑93 (MQ=255)

                                                                          gCGGGTAAGCCTGTGGCTGTATCGATGGGCAGCATGGCGGCATCTGTGGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAAcccc              <  1:2789302/93‑1 (MQ=255)

                                                                          gCGGGTAAGCATGGGGCTGTATCGATGGGCGGGATGGCGGGATCTGGTGGTTACTGGATTGCGACGCCAGATACTTACATTGTGGCTAAccag              >  2:3805306/1‑91 (MQ=255)

                                                                            gggTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAg            <  1:635200/93‑1 (MQ=255)

                                                                              gTAAGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCa          >  2:5146803/1‑93 (MQ=255)

                                                                               gaaGCCTGTGGGTGTATCGATGGGCGGCATGGCCGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCAc         <  1:7198719/92‑1 (MQ=255)

                                                                                 aGCCTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCAccc       >  1:7078059/1‑93 (MQ=255)

                                                                                   ccTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTg     <  2:1904917/93‑1 (MQ=255)

                                                                                    cTGTGGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGa    >  2:1328099/1‑93 (MQ=255)

                                                                                     tgtgGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGAc   <  1:3072958/93‑1 (MQ=255)

                                                                                      gtgGCTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGAcc  <  1:6118107/93‑1 (MQ=255)

                                                                                      gtgGATGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCATCTAATTACATTGTGGCTAACCCCAGCACCCTGAcc  <  1:3288838/93‑1 (MQ=255)

TGCGTGTTAATAGCCCAGGCGGCAGCGTTACCGCGTCTGAAGTGATTCGCGCTGAACTGGCAGCAGCCCGGGCAGCGGGTAAGCCTGTGGTTGTATCGATGGGCGGCATGGCGGCATCTGGTGGTTACTGGATTTCCACGCCAGCTAATTACATTGTGGCTAACCCCAGCACCCTGACC  >  NC_000913/1847969‑1848147

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`