BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,862,301	T→C	V143A (GTG→GCG)	yeaD →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,862,301	0	T	C	99.7%	1059.6 / ‑4.9	307	V143A (GTG→GCG)	yeaD	hypothetical protein
Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (143/163); total (144/163)
Fisher's exact test for biased strand distribution p-value = 4.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01

Only 100 of 320 total aligned reads displayed.

ATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCG  >  NC_000913/1862211‑1862390

aTGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCgg                                                                                         <  1:7943139/93‑1 (MQ=255)

aTGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCgg                                                                                         >  1:2048205/1‑93 (MQ=255)

  ggCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGt                                                                                       <  1:8205988/93‑1 (MQ=255)

   gCGTAGCGCTTACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTa                                                                                      <  1:2255180/93‑1 (MQ=255)

   gCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTa                                                                                      <  1:4960244/93‑1 (MQ=255)

     gTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTaaa                                                                                    <  1:4649082/93‑1 (MQ=255)

       aGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAAc                                                                                  >  1:6278317/1‑93 (MQ=255)

        gcgcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACt                                                                                 <  1:862562/93‑1 (MQ=255)

        gcgcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACt                                                                                 <  1:8000365/93‑1 (MQ=255)

        gcgcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACt                                                                                 <  1:3697224/93‑1 (MQ=255)

        gcgcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACt                                                                                 <  2:1673024/93‑1 (MQ=255)

          gcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTtg                                                                               <  1:8283637/93‑1 (MQ=255)

          gcTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTtg                                                                               <  2:3077647/93‑1 (MQ=255)

             gACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGa                                                                            >  1:7509358/1‑93 (MQ=255)

               cTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGaaa                                                                          <  2:730934/93‑1 (MQ=255)

                ttttGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAAt                                                                         >  1:698441/1‑93 (MQ=255)

                ttttGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAAt                                                                         <  2:7460252/93‑1 (MQ=255)

                 tttGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATc                                                                        <  2:4940721/93‑1 (MQ=255)

                  ttGAATTGACGCAAAGCGAAGAGACGAAAAAATGCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCg                                                                       <  1:8467628/93‑1 (MQ=255)

                   tgaatgaaCGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGa                                                                      <  1:6346520/93‑1 (MQ=255)

                    gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGAt                                                                     >  1:3025243/1‑93 (MQ=255)

                    gAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGAt                                                                     <  2:8284391/93‑1 (MQ=255)

                     aaTTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATc                                                                    <  1:6871433/93‑1 (MQ=255)

                     aaTTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATc                                                                    >  1:3307113/1‑93 (MQ=255)

                     aaTTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATc                                                                    <  1:7231497/93‑1 (MQ=255)

                     aaTTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATc                                                                    <  2:6108643/93‑1 (MQ=255)

                          aCGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGaa                                                               <  1:3512235/93‑1 (MQ=255)

                            gCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATc                                                             <  1:7702520/93‑1 (MQ=255)

                              aaaGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATcac                                                           <  1:3946539/93‑1 (MQ=255)

                               aaGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATcaca                                                          <  2:2130278/93‑1 (MQ=255)

                                aGCGAAGAGACGAAAAAATTCTGGCCTCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACAt                                                         <  2:889681/93‑1 (MQ=255)

                                aGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACAt                                                         <  1:907310/93‑1 (MQ=255)

                                 gCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATg                                                        <  2:5941237/93‑1 (MQ=255)

                                 gCGAAGAGACGAAAAAATGCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATg                                                        <  1:5548801/93‑1 (MQ=255)

                                  cGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATgg                                                       <  2:4628379/93‑1 (MQ=255)

                                  cGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATgg                                                       <  2:6471091/93‑1 (MQ=255)

                                   gAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGc                                                      <  1:6480561/93‑1 (MQ=255)

                                   gAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGc                                                      >  1:2208189/1‑93 (MQ=255)

                                   gAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGc                                                      >  1:15237/1‑93 (MQ=255)

                                   gAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGc                                                      <  1:3418979/93‑1 (MQ=255)

                                   gAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGc                                                      <  1:139023/93‑1 (MQ=255)

                                    aaGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCg                                                     >  2:571782/1‑93 (MQ=255)

                                    aaGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCg                                                     >  1:4081311/1‑93 (MQ=255)

                                     tgagaCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGa                                                    >  2:1360905/2‑93 (MQ=255)

                                     agagACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGa                                                    >  2:6636758/1‑93 (MQ=255)

                                      gagaCGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGaa                                                   >  2:6176426/1‑93 (MQ=255)

                                           gAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGa                                              >  2:7364732/1‑93 (MQ=255)

                                            aaaaaaTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGaa                                             <  1:1022713/93‑1 (MQ=255)

                                            aaaaaaTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGaa                                             >  1:3993529/1‑93 (MQ=255)

                                             aaaaaTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGaaa                                            <  2:8044744/93‑1 (MQ=255)

                                                 aTTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAaccac                                        <  1:5153773/93‑1 (MQ=255)

                                                   tCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACct                                      <  2:3435705/93‑1 (MQ=255)

                                                   tCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACct                                      <  1:7550077/93‑1 (MQ=255)

                                                    cTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCCCctc                                     <  2:2376371/93‑1 (MQ=255)

                                                    cTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACctc                                     <  2:3244533/93‑1 (MQ=255)

                                                    cTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACctc                                     <  1:5684160/93‑1 (MQ=255)

                                                    cTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACctc                                     <  1:3182174/93‑1 (MQ=255)

                                                     tGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACctct                                    >  2:1427169/1‑93 (MQ=255)

                                                     tGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACAACctct                                    >  2:4870893/1‑93 (MQ=255)

                                                      ggCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTg                                   <  2:4010097/93‑1 (MQ=255)

                                                      ggCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTg                                   >  2:5186925/1‑93 (MQ=255)

                                                       gCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGc                                  >  1:2824238/1‑93 (MQ=255)

                                                        ccGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGcc                                 <  1:4829606/93‑1 (MQ=255)

                                                         cGCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGccc                                <  1:266551/93‑1 (MQ=255)

                                                          gCACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCt                               >  2:8683223/1‑93 (MQ=255)

                                                           cACGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTg                              >  2:6951356/1‑93 (MQ=255)

                                                            aCGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGc                             <  2:5103566/93‑1 (MQ=255)

                                                            aCGACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGc                             >  2:7583474/1‑93 (MQ=255)

                                                              gACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGTCGAATTTGAAACCACCTCTGCCCTGCAt                           >  1:1236490/1‑93 (MQ=255)

                                                              gACTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCAt                           >  2:7090601/1‑93 (MQ=255)

                                                               aCTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCTATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATa                          <  2:2284372/93‑1 (MQ=255)

                                                               aCTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAACCCCCCTCTGCCCTGCATa                          <  2:3189264/93‑1 (MQ=255)

                                                               aCTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATa                          <  1:5901174/93‑1 (MQ=255)

                                                               aCTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATa                          <  2:284883/93‑1 (MQ=255)

                                                               aCTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATa                          >  2:2198792/1‑93 (MQ=255)

                                                               aCTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATa                          <  2:2628408/93‑1 (MQ=255)

                                                                cTTTACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAc                         <  2:5299776/93‑1 (MQ=255)

                                                                cTTTACGCTGTTAGCGCAGTTCCGCGCGGGGAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCCTCTCTGCCCTACATAc                         >  2:274337/1‑93 (MQ=255)

                                                                 tttACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAcc                        >  1:6197239/1‑93 (MQ=255)

                                                                 tttACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAcc                        <  1:6656915/93‑1 (MQ=255)

                                                                 tttACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAcc                        >  2:3773721/1‑93 (MQ=255)

                                                                 tttACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAcc                        >  2:632039/1‑93 (MQ=255)

                                                                 tttACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATAcc                        >  1:1846143/1‑93 (MQ=255)

                                                                  ttACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCt                       <  1:1137630/93‑1 (MQ=255)

                                                                  ttACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCt                       >  1:4421359/1‑93 (MQ=255)

                                                                   tACGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTa                      >  2:4520601/1‑93 (MQ=255)

                                                                    aCGCTGTTAGCGCATTTCCGCGCGGGTAAAACTGGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTAc                     <  1:8486262/93‑1 (MQ=255)

                                                                     cGCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACt                    >  2:1921402/1‑93 (MQ=255)

                                                                      gCTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTCAAACCACCTCTGCCCTGCATACCTACtt                   >  1:2481966/1‑93 (MQ=255)

                                                                       cTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACttt                  <  1:1940206/93‑1 (MQ=255)

                                                                       cTGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACttt                  >  2:8508228/1‑93 (MQ=255)

                                                                        tGTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTa                 >  2:4311320/1‑93 (MQ=255)

                                                                         gTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTaa                <  2:1164824/93‑1 (MQ=255)

                                                                         gTTAGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTaa                <  1:4514304/93‑1 (MQ=255)

                                                                            aGCGCATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGt             <  2:1572638/93‑1 (MQ=255)

                                                                             gcgcATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTg            >  1:290161/1‑93 (MQ=255)

                                                                                cATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGt         <  2:1217420/93‑1 (MQ=255)

                                                                                cATTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGt         >  2:4233060/1‑93 (MQ=255)

                                                                                 aTTTCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTg        >  2:5437819/1‑93 (MQ=255)

                                                                                  tttCCGCGCGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTCGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGa       >  2:3339372/1‑93 (MQ=255)

ATGGCGTAGCGCTGACTTTTGAATTGACGCAAAGCGAAGAGACGAAAAAATTCTGGCCGCACGACTTTACGCTGTTAGCGCATTTCCGCGTGGGTAAAACTTGTGAAATCGATCTTGAATCACATGGCGAATTTGAAACCACCTCTGCCCTGCATACCTACTTTAACGTGGGTGATATCG  >  NC_000913/1862211‑1862390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`